The relatively poor accessibility, high costs, long turnaround time for analysis, and lack of information about soil and plant testing in the Andean highland region (Altiplano) of Bolivia has hampered informed and timely nutrient management decisions for potato (Solanum tuberosum L.) crops. The objective of this study was to determine if the Cardy nitrate meter (Horiba Ltd., Kyoto, Japan), a low-cost, portable, and rapid field test method, could be used to improve nitrogen (N) fertility management among isolated rural indigenous communities at different elevations in this rugged semi-arid environment. Fully matured leaf petioles were sampled at blooming time from potato trials established in three communities during the 2006–2007 and 2007–2008 growing seasons. Those trials were composed of 12 fertility treatments (i.e., an unfertilized control and different combinations of sheep and cow manure, inorganic fertilizer, commercial household/urban compost, and a commercial microbial activator soil amendment) arranged in a randomized complete block design with four replications. In general, in both growing seasons and in all communities, nitrate measured in the sap of leaf petioles by the Cardy meter had relatively low but significant correlations with leaf petiole total N (ranging from r2 = 0.07 to r2 = 0.60 across seasons and communities) and with total fresh tuber yield (ranging from r2 = 0.11 to r2 = 0.50 across seasons and communities). However, some problems were encountered with being able to consistently calibrate the meter with supplied standards prior to each use of the meter. These results indicate that the Cardy ion meter might be an effective tool for potato growers or agricultural professionals working in the Altiplano of Bolivia if further research can be conducted to determine if the meter is consistent for diverse sites in the Altiplano and is practical for the cultural, economic, and environmental conditions of the region.