One of the unique ornamental chickens that is in great demand by ornamental chicken enthusiasts is the long-tailed chicken or onagadori chicken originating from the city of Nankoku Kochi, Japan. This study was aimed to determine the effect of the frequency of semen collection on spermatozoa quality in onagadori chickens. In the study used a completely randomized design (CRD) with three treatment groups using eight onagadori chickens with an age range of 6-7 months. The first treatment (P1), was cement collection carried out every six days or once a week; the second treatment (P2) was cement collection carried out every three days or twice a week; the third treatment (P3) was cement collection carried out every two days or thrice a week. Each treatment was repeated three times. Observations were made on the quality of onagadori chicken spermatozoa. Semen collection once a week produces a volume of 0.087 mL; concentration 4.7 billion cells/mL; motility 84.66%; abnormality 8.46%; viability 81.44%. Semen collection twice a week produces a volume of 0.09 mL; concentration 4.9 billion cells/mL; motility 83.22%; abnormality 9.1%; viability 83.87. Semen collection three times a week produces a volume of 0.09 mL; concentration 4.7 billion cells/mL; motility 83.57%; abnormality 8.54%; viability 82.32%. The data obtained was analyzed by Analysis of Variant and then followed by the Least Significant Difference (LSD) test. The results showed that the frequency of semen collection at P1, P2, and P3 on volume and concentration, motility, and abnormalities yielded no significant differences (P>0.05), while viability showed significant differences (P<0.05).