Event Abstract Back to Event Changes in the spontaneous EEG and locomotor activity of freely moving rats in mitragynine-treated animals. Zurina Hassan1*, Zarif M. Sofian1 and Visweswaran Navaratnam1 1 Universiti Sains Malaysia, Centre for Drug Research, Malaysia Mitragyna speciosa or locally known as “Ketum” is a psychoactive plant preparation used in Southeast Asia. Traditionally, it has been used due to its opium-like effect, to counter the addiction to heroin and act as stimulant to help labourers endure physical fatigue. The active alkaloid of the plant is mitragynine. The aim of the study is to determine the neurological and psychiatric disorder of the animal treated with different doses of mitragynine. Using wireless headstage system, the EEG activity was recorded from hippocampal region of the brain in freely moving rats. The activity was recorded, amplified, filtered (band pass filters to separate and display the frequency bands: Delta, 0.1-3.5 Hz; Theta, 3.6-7.5 Hz; Alpha, 8-13 Hz; Beta, 14-30 Hz) and digitized (200Hz) by PowerLab/4s system. The EEG activity and locomotor activity were recorded spontaneously. Single dose administration of methamphetamine (1mg/kg) has shown significant increases in large amplitude; low frequency rhythms in the EEG of rats (i.e. increase in delta wave). This dose also increased the locomotor activity and stereotypic behavioural such as licking, biting and head waving. Mitragynine (1 and 10mg/kg) was found to significantly suppress large amplitude, low frequency rhythms in the EEG of rats (i.e. decrease in delta wave). The same results were observed in the morphine (5mg/kg). Mitragynine also showed hypolocomotion and no significant difference in stereotypic behaviour compared to control. In conclusion, the possible mechanism to be involved in suppressing the large amplitude as in mitragynine may be cholinergic, monoaminergic or serotonergic system. Acknowledgements Financial support is received from the Higher Education Centre of Excellence (HICoE) Research and Innovation, Ministry of Higher Education (MOHE) Malaysia, with facilities from the Universiti Sains Malaysia (304/CDADAH/650527/K134). Keywords: mitragynine, EEG activity, locomotor activity, Methamphetamine, Morphine Conference: ACNS-2012 Australasian Cognitive Neuroscience Conference, Brisbane, Australia, 29 Nov - 2 Dec, 2012. Presentation Type: Poster Presentation Topic: Other Citation: Hassan Z, Sofian ZM and Navaratnam V (2012). Changes in the spontaneous EEG and locomotor activity of freely moving rats in mitragynine-treated animals.. Conference Abstract: ACNS-2012 Australasian Cognitive Neuroscience Conference. doi: 10.3389/conf.fnhum.2012.208.00042 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 25 Oct 2012; Published Online: 07 Nov 2012. * Correspondence: Dr. Zurina Hassan, Universiti Sains Malaysia, Centre for Drug Research, Gelugor, Penang, 11800, Malaysia, zurina_hassan@usm.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Zurina Hassan Zarif M Sofian Visweswaran Navaratnam Google Zurina Hassan Zarif M Sofian Visweswaran Navaratnam Google Scholar Zurina Hassan Zarif M Sofian Visweswaran Navaratnam PubMed Zurina Hassan Zarif M Sofian Visweswaran Navaratnam Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.