Deterioration of reinforced and prestressed concrete structures is one of the main challenges in structural engineering. As the majority of our infrastructure is built around 1960-1980, most structures have reached or will soon reach their design lifetime, giving rise to more structural problems and costs in the coming decades. It is therefore important to develop efficient assessment schemes allowing to assure the safety and reliability of these existing structures and to estimate their residual lifetime. Within the framework of the Bridge|50 research project, a 50-year-old prestressed concrete bridge showing signs of corrosion damage was decommissioned. The bridge girders were recovered and have been subjected to large-scale load tests with varying loading patterns. This paper presents the results acquired during acoustic emission (AE) monitoring of one of the I-shaped girders during a monotonic three-point bending test with an eccentric point load. The results show that the failure mode and location could be detected based on AE activity and zonal AE localization. The onset of concrete cracking was observed by a decrease in peak frequency of the AE signals. Average frequency (AF) and rise angle (RA) value analysis was used to characterize the crack mode, which shifted from tensile to shear mode during bending.