Objectives. This study aimed to determine the effect of risk factors associated with non-specific neck pain on neck disability among computer users. Methods. A cross-sectional observational study was conducted with 1028 office workers with neck pain. Data were collected using questionnaires, which included the Neck Disability Index, Numerical Pain Rating Scale, Four-Item Patient Health Questionnaire, demographic information, frequency of neck and head pain. Multinomial logistic regression analysis was performed. Results. Age (odds ratio[OR] 1.08, 95% confidence interval[CI] [1.05, 1.10]), female gender (OR 1.85, 95% CI [1.34, 2.56]), body mass index (OR 1.04, 95% CI [1.01, 1.07]), work experience 5–10 years (OR 3.29, 95% CI [2.19, 4.95]) or >10 years (OR 2.70, 95% CI [1.90, 3.83]), daily computer usage time >8 h (OR 2.40, 95% CI [1.41, 4.07]), monitor located outside the midline (OR 2.11, 95% CI [1.48, 3.01]) and stress level mild (OR 3.10, 95% CI [1.88, 5.11]), moderate (OR 7.81, 95% CI [4.50, 13.55]) or severe (OR 9.11, 95% CI [5.20, 15.94]) were identified as significant risk factors for neck disability. Conclusion. This information may enable designing appropriate workstations for preventing neck disability and prevent labour losses. Further research is needed to help us understand more about neck disability. Trial registration ClinicalTrials.gov identifier: NCT04821024.