Alzheimer's disease (AD) is the leading cause of dementia. Several studies indicate a possible relationship between different genes and Alzheimer's disease. To further investigate, we have analyzed the association between the bleomycin hydrolase (BLMH) and apolipoprotein E (ApoE) polymorphisms in 93 AD patients and age- and sex-matched 113 controls from the Tunisian population. The frequency of ApoE epsilon 4 allele was found to differ significantly in AD patients compared to the control [29.5% vs. 8.8 (χ (2)=26, df=1, p<0.001)] leading to an increased risk of AD in subjects with this allele (OR=3.29, 95% CI=1.7-6.5; p=0.001]. This risk was found to decrease from OR=8.4, CI=3.3-23; p<0.001 in subjects less than 75years old to OR=1.2, CI=1.031-14; p=0.0297 in subjects 75years and older. No association was observed between carrying the BLMH-G genotype and AD in ε4 negative or positive subjects.