Event Abstract Back to Event High-sensitivity, high-specificity, and wash-free detections of bio-molecules via immunomagnetic reduction Herng-Er Horng1*, Shang-Hsun Yang1, Chin-Yih Hong2, Hong-Chang Yang3 and Chau-Chung Wu2 1 National Taiwan Normal University, Institute of Electro-optical Science and Technology, Taiwan 2 National Chung Hsing University, Graduate Institute of Biomedical Engineering, Taiwan 3 National Taiwan University, Department of Physics, Taiwan By conjugating antibodies onto magnetic nanoparticles, magnetic nanoparticles become bio-functional and are able to associate with specific bio-entities, such as proteins, virus, or nuclei acids. Through the association, the ac magnetic susceptibility of magnetic particles dispersed in water is reduced. The concentration of the detected bio-entities in a sample can be probed by measuring the reduction in the ac magnetic susceptibility of magnetic reagent. To achieve high-sensitivity assays, a SQUID-based magnetosusceptometry is developed to detect the reduction in the magnetic susceptibility of magnetic reagent as magnetic nanoparticles associate with target bio-entities. In addition to show the designed and specifications of the SQUID magnetosusceptometry, the characterizations of assaying proteins, viruses, and nuclei acids using the SQUID magnetosusceptometry are explored. It was found that the low-detection limit is 1 pg/ml or less, depending on the detected bio-molecules. Furthermore, the cross reaction between antibodies and non-specific bio-molecules can be depressed significantly by well adjusting the frequency of ac magnetic fields. Conference: Biomag 2010 - 17th International Conference on Biomagnetism , Dubrovnik, Croatia, 28 Mar - 1 Apr, 2010. Presentation Type: Poster Presentation Topic: Nanoparticles, Biosusceptometry and Effects of Magnetic Fields Citation: Horng H, Yang S, Hong C, Yang H and Wu C (2010). High-sensitivity, high-specificity, and wash-free detections of bio-molecules via immunomagnetic reduction. Front. Neurosci. Conference Abstract: Biomag 2010 - 17th International Conference on Biomagnetism . doi: 10.3389/conf.fnins.2010.06.00383 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Apr 2010; Published Online: 08 Apr 2010. * Correspondence: Herng-Er Horng, National Taiwan Normal University, Institute of Electro-optical Science and Technology, Taipei, Taiwan, phyfv001@scc.ntnu.edu.tw Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Herng-Er Horng Shang-Hsun Yang Chin-Yih Hong Hong-Chang Yang Chau-Chung Wu Google Herng-Er Horng Shang-Hsun Yang Chin-Yih Hong Hong-Chang Yang Chau-Chung Wu Google Scholar Herng-Er Horng Shang-Hsun Yang Chin-Yih Hong Hong-Chang Yang Chau-Chung Wu PubMed Herng-Er Horng Shang-Hsun Yang Chin-Yih Hong Hong-Chang Yang Chau-Chung Wu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.