We analyzed chromosome aberrations, micronucleus frequency, mitotic index (MI), and nuclear division index (NDI) in peripheral lymphocytes of sheep subchronically exposed to the fungicide Euparen Multi (containing 50% tolylfluanid). Euparen Multi was administered by rumen sonde to group of Merino sheep (seven sheep/group) at 93 mg/kg body weight (1/20 LD 50) daily for 28 days to assess its genotoxic effects. The frequencies of aberrant cells (ABC) in the experimental and control groups were 5.50±1.38% and 2.40±1.14%, respectively, and the increase in ABC in the treated group was significant ( P = 0.003 ). Significantly increased numbers of chromatid breaks (5.67±1.21% against 2.40±1.14%; P = 0.001 ), chromatid gaps (10.33±2.73% against 4.00±1.23%; P = 0.001 ), and chromosome gaps (1.83±0.75% against 0.80±0.45%; P = 0.025 ) and exchanges (3.17±1.94% against 0.20±0.45%; P = 0.009 ) were observed in exposed animals in comparison to control animals. The frequency of micronuclei (MN) was 29.40±5.86 per 1000 binucleated cells in peripheral lymphocytes of sheep in the control group and 49.57±19.12 per 1000 binucleated cells in the treated group. A significant increase in the frequency of MN in peripheral lymphocytes also was observed between the two groups ( P = 0.0477 ). No statistical differences in MI and NDI values were found in the groups ( P = 0.181 and 0.761, respectively). Thus, our results suggest that exposure to Euparen Multi may cause genome damage in somatic cells.