Background: There has been some growing evidence that Vitamin D deficiency is also a causative factor in the etiology of depression, but there hasn’t been much work done on this topic and the evidence is inconsistent. The objective of this research is to find the association between blood 25-hydroxyvitamin D [25(OH)D] concentrations and depression among patients with depression in our local population. Objective: To determine frequency of vitamin D deficiency in patient with depression. Study design: Cross-sectional study Place and Duration of Study: Department of Psychiatry, Institute of Psychiatry, Benazir Bhutto Hospital, Rawalpindi from 3rd August 2017 to 2nd February 2018. Methodology: One hundred and forty-two clinically diagnosed cases of depression gender between age 18 to 60 years were enrolled. Serum Vitamin D levels were estimated in each patient and frequency of vitamin D deficiency was determined. Results: 51 (35.9%) patients reported to have vitamin-D deficiency. Conclusions: We recommend effective screening tests for early detection vitamin D deficiency in clinically diagnosed patients of depression. Keywords: Depression, Vitamin D, Vitamin D deficiency