In this paper, 25-ton and 27-ton axle heavy trucks are used to carry out moving loading and dynamic real vehicle test on the cracked section, the intact section, and the repaired section of a railway tunnel foundation to test the dynamic performance of the tunnel basement structure with the change of axle loads and health conditions. By analyzing the influence law of dynamic response and fatigue life of heavy haul train under different basement conditions (intact, damaged, and repaired), the adaptability of railway tunnel equipment to freight trucks axle load is clarified. The results show that (1) the intact section of the tunnel can meet the normal operation of 25-ton and 27-ton axle load freight trains in good condition. (2) The normal operation of 25-ton and 27-ton axle load freight trucks is seriously affected by the cracked section of the tunnel. When the cracks in the tunnel basement are gradually hollowed out by groundwater, serious traffic accidents such as vehicle shaking and derailment are likely to occur. (3) The repaired section of the tunnel can meet the normal operation of 25-ton and 27-ton axle load freight trains after adopting the integrated comprehensive treatment of “Anchor-Injection-Drainage”. The research results will have reference significance for the condition assessment and disease treatment of the basement structure of the heavy haul railway tunnel.