Freezing Behavior Research Articles

Effect of pore precipitation freezing in different saturated states on mechanical performance for drainage asphalt pavement

To explore the effect of pore precipitation freezing in different saturated states on mechanical performance for drainage asphalt pavement, the study revealed the freezing behavior characteristics of pore precipitation in different saturated states by combining CT scanning, FEM simulation and mechanical tests. Firstly, the real spatial structure of drainage asphalt mixture was obtained through the CT scanning, and the corresponding FEM simulation model was established. Secondly, the process of pore precipitation freezing in different saturated states was simulated and analyzed, and its influence on the spatial structure of drainage asphalt mixture was obtained. Finally, the effect of pore precipitation freezing in different saturated states on drainage asphalt mixture was proved through mechanical tests. It is found that the effect of pore precipitation freezing on mechanical performance of drainage asphalt mixture is different under different saturated states. With the increase of saturation state, the influence of pore precipitation freezing becomes more obvious, and the difference of stress-strain distribution in spatial structure for drainage asphalt mixture is more prominent. The study explains how pore precipitation freezing in different saturated states affects the mechanical performance for drainage asphalt pavement, providing a theoretical basis for enhancing its durability.

Chemogenetic activation of CRF neurons as a model of chronic stress produces sex-specific physiological and behavioral effects

Trauma and chronic stress exposure are the strongest predictors of lifetime neuropsychiatric disease presentation. These disorders often have significant sex biases, with females having higher incidences of affective disorders such as major depression, anxiety, and PTSD. Understanding the mechanisms by which stress exposure heightens disease vulnerability is essential for developing novel interventions. Current rodent stress models consist of a battery of sensory, homeostatic, and psychological stressors that are ultimately integrated by corticotropin-releasing factor (CRF) neurons to trigger corticosteroid release. These stress paradigms, however, often differ between research groups in the type, timing, and duration of stressors utilized. These inconsistencies, along with the variability of individual animals’ perception and response to each stressor, present challenges for reproducibility and translational relevance. Here, we hypothesized that a more direct approach using chemogenetic activation of CRF neurons would recapitulate the effects of traditional stress paradigms and provide a high-throughput method for examining stress-relevant phenotypes. Using a transgenic approach to express the Gq-coupled Designer Receptor Exclusively Activated by Designer Drugs (DREADD) receptor hM3Dq in CRF-neurons, we found that the DREADD ligand clozapine-N-oxide (CNO) produced an acute and robust activation of the hypothalamic-pituitary-adrenal (HPA) axis, as predicted. Interestingly, chronic treatment with this method of direct CRF activation uncovered a novel sex-specific dissociation of glucocorticoid levels with stress-related outcomes. Despite hM3Dq-expressing females producing greater corticosterone levels in response to CNO than males, hM3Dq-expressing males showed significant typical physiological stress sensitivity with reductions in body and thymus weights. hM3Dq-expressing females while resistant to the physiological effects of chronic CRF activation, showed significant increases in baseline and fear-conditioned freezing behaviors. These data establish a novel mouse model for interrogating stress-relevant phenotypes and highlight sex-specific stress circuitry distinct for physiological and limbic control that may underlie disease risk.

Numerical Analysis of the Freezing Behavior of Saturated Cementitious Materials with Different Amounts of Chloride.

The freezing behavior of cement paste saturated with different chloride concentrations is investigated numerically with a coupled 3D hygro-thermo-mechanical FE analysis. The mathematical formulation of the freezing processes in the context of poromechanics takes into account the water (hydraulic) and ice pore pressures, as well as the distribution of heat (temperature) and strains. These quantities are calculated numerically based on three coupled differential equations, namely the static equilibrium equation and the equations for the transport of water and heat. The coupling between the mechanical (loading) and the non-mechanical processes (freezing) is performed using a staggered solution scheme. The proposed numerical approach is first validated using numerical and experimental studies from the literature dealing with two different cement pastes saturated with different amounts of chloride. The validated model is then used to investigate the effects of liquid water permeability, total porosity and pore size distribution on the freezing behavior of hardened cement paste. The results show that liquid water permeability has a strong effect on the pore pressure and deformation of the hardened cement paste. It is also shown that by decreasing the total porosity, the material becomes denser and contracts more as the temperature decreases, leading to a decrease in freezing strain. The results of this paper will provide important findings for the development of a simplified engineering model to investigate the mechanism that leads to freeze-thaw salt-induced damage to concrete structures in the framework of the DFG-funded research project.

Sex difference in the facilitation of fear learning by prior fear conditioning

There is now ample evidence that the strength and underlying mechanisms of memory formation can be drastically altered by prior experience. However, the prior work using rodent models on this topic has used only males as subjects, and as a result, we do know whether or not the effects of prior experience on subsequent learning are similar in both sexes. As a first step towards addressing this shortcoming, rats of both sexes were given auditory fear conditioning, or fear conditioning with unsignaled shocks, followed an hour or a day later by a single pairing of light and shock. Fear memory for each experience was assessed by measuring freezing behavior to the auditory cue and fear-potentiated startle to the light. Results showed that males trained with auditory fear conditioning showed facilitated learning to the subsequent visual fear conditioning session when the two training sessions were separated by one hour or one day. Females showed evidence of facilitation in rats given auditory conditioning when they were spaced by an hour but not when they were spaced by one day. Contextual fear conditioning did not support the facilitation of subsequent learning under any conditions. These results indicate that the mechanism by which prior fear conditioning facilitates subsequent learning differs between sexes, and they set the stage for mechanistic studies to understand the neurobiological basis of this sex difference.

Thermo-hydro-mechanical coupled model of unsaturated frozen soil considering frost heave and thaw settlement

Frost heave and thaw settlement of subgrade is the most typical engineering disease in permafrost regions, which seriously threatens the safe construction and operation of transportation infrastructure. On the basis of the Modified Cam Clay model, a thermoelastoplastic constitutive model of unsaturated frozen soil is proposed. This model can effectively describe two key issues of the mechanical behavior of frozen soils, that is, soil hardening caused by temperature reduction and volume compression during thawing. The proposed constitutive model is realized in the established thermo-hydro-mechanical (THM) coupled mathematical model, and the reliability of the coupled model is verified by comparing with the experimental results. In addition, the proposed THM framework is applied to describe the long-term freezing and thawing behavior of typical railway subgrade sections in the permafrost region of western China. The results show that water-heat transfer and deformation frequently occur on the subgrade surface as the seasons change. Moreover, the variation of thaw settlement is greater than that of frost heave, which seriously threatens the operation safety of railways in permafrost regions.

Associations between immune competence phenotype and stress response in sheep

Immune competence in domestic sheep is gaining more attention in genetic selection programs that seek to enhance flock immunity and animal welfare. A goal of many programs is to identify behavioral phenotypes that indicate stress-coping strength, as well as disease resilience. The current study set out to explore the relationship between immune responsiveness, physiological stress, and behavioral response among sheep that had been selected for ‘low’ (LR) or ‘high’ (HR) response to cell-mediated and antibody-mediate reactivity to a clostridial vaccine. Multiparous ewes were placed in four experiments which exposed the animals to various threats including, dog presence, human proximity, visual isolation from flock, and physical restraint. To evaluate the consistency of behavioral phenotypes, all ewes were placed in the test circuit one year later. Basal body temperature (via iButton) and serum cortisol concentrations were collected prior to and after the circuit each year. Immune group (HR vs. LR) was not found to be related to behavioral performance during the dog, human, isolation, or restraint challenge. Immune group categorization was also unrelated to pre- and post-cortisol concentrations, and the change in cortisol concentrations during testing. There was a negative relationship identified between response to visual isolation and change in cortisol response during testing, indicating that sheep which were more active or agitated during visual isolation from the flock experienced less of an increase in serum cortisol levels and were perhaps experiencing a negative, high arousal state compared to less reactive sheep (e.g., freeze behavior) (χ2(4, N=99) = 42.72, P <0.0001). There was also a post hoc, positive relationship identified between weight measurements and immune group, such that individuals with greater body weight were more likely to be in the high immune responsiveness group (P= 0.01). Specifically, for every unit increase in weight, there was a ~49% chance of being categorized in the HR group. This is relevant for selection programs because producers that seek to enhance immune responsiveness and performance may be able to select sheep that carry a greater body mass, and while not found in the present study, could lead to greater flock immunity.

Freezing behaviors of an impacting droplet on subcooled hydrophobic surfaces

The solidification of impacting water droplets on cold substrates wildly exists in nature and engineering devices, which would significantly affect their performances. The freezing mechanisms of impacting water droplets on hydrophobic surfaces are not well understood. In this work, we explore the freezing behaviors of impacting water droplets on a cold hydrophobic surface in the regime where the interaction between the droplet impact dynamics and solidification is strong. We observe six different freezing morphologies on hydrophobic surfaces via tuning the substrate temperature and impact velocity. The diverse freezing morphologies are resulted from the competition of timescales associated with the solidification and droplet impact dynamics. The final freezing area on surfaces shows a non-monotonic variation with impact height when Ts = -38° C. We thus developed a model to predict the final freezing area, which agrees well with the experimental results.

Percolation characteristics of pore fluid during hydrate depressurization dissociation from multi-phase multi-field coupling analysis

The recovery of gas from hydrate-bearing reservoirs is essentially controlled by pore fluid flow. Four numerical models with different multi-phase multi-field were established in this work based on the thermo-hydro-chemical model, considering ice phase, fluidized sand phase, depositing sand phase, and ice, fluidized sand and depositing sand phases, respectively. These above models were used to simulate the dissociation of hydrate-bearing cores by depressurization and to investigate the effects of freezing and melting behavior, sand production behavior, and their combined behavior on pore fluid migration. By comparing the simulations with and without the ice phase, it can be found that the permeability decreases sharply due to ice generation resulting in a decrease of 89.16% in pore-water velocity and of 99.95% in pore-gas velocity. This indicates an inhibition of pore fluid migration by freezing behavior. Meanwhile, it can be found that the ice melting behavior can temporarily promote pore-water migration but has no obvious promotion for pore-gas migration. It is difficult for the pore fluid to percolate in the regions prone to ice generation during hydrate dissociation. In addition, the permeability at 300 min with sand detachment is 16% higher than that without sand detachment. But at the same time, the pore water velocity is 28.44% smaller since sand detachment behavior does not directly power pore fluid migration. By comparing the simulations with and without the combined behavior, it can be concluded that the freezing behavior and the sand production behavior can jointly weaken the pore fluid mobility. Significantly, the former has a stronger negative effect on the pore fluid mobility than the latter during the depressurization dissociation of the hydrate-bearing sediment.

Physical origins of freezing and melting temperature depressions of water in millimeter-sized pores

As a long-standing problem, the physics of freezing and melting temperature depressions of water in millimeter-sized pores remains elusive. We devised a series of experiments on the freezing and melting of water in glass tubes with varying tube diameters, water volumes, water-solid interfacial areas, and cooling rates. The results of the experiments indicate that the freezing temperature depression is highly correlated with the water-solid interfacial areas. It can be inferred that heterogeneous nucleation is the dominant physical origin for such freezing temperature depression. In contrast, melting temperature depression is orders of magnitude lower than that of freezing, and is independent of heating rate, water volume, water-solid interfacial area, and correlated with pore diameter, suggesting the governing role of capillarity. The recognized distinct physical origins of freezing and melting temperatures lead to the disparity between them, i.e., freezing and melting hysteresis. The findings resolve the controversy on the freezing mechanism of water in millimeter-sized pores, and can be used to better manipulate freezing and melting behaviors of pore water in various engineering applications, e.g., promoting or inhibiting freezing in biomaterials, and developing anti-freezing materials.

A novel automated software and video analysis system for quantifying physiological responses of conditioned fear in mice

BACKGROUND: Conditioned fear is an aversive learning process that is highly sensitive to sensory conditions such as environmental context, threat intensity and circadian rhythms. To better understand these external variables that impact conditioned fear learning, we developed a novel software application that allows for programmable remote conditioned stimulus (CS, ie., auditory tone) delivery and recording of group-housed mice in a homecage, or familiar environment. The goal of our study was therefore to leverage this technology to examine conditioned fear in the homecage across light-dark (LD) circadian cycles. METHODS: Male C57bl/6J mice underwent Pavlovian auditory fear conditioning (20 CS/US pairings), with a CS-only group as a control (n=4/group). Mice were then returned to the homecage, equipped with speaker and camera. Using a customized open-source software to remotely evoke an enhanced physiological state of fear in the homecage, mice received a select number of conditioned auditory stimuli (30sec. intermittent tones) throughout the LD cycle over 14 days. The software was configured to deliver six tones (6kHz) over a 24hr period (3 light, 3 dark). The first CS during each LD cycle was recorded and percent freezing behavior was manually scored. On day 15, to evaluate whether the HC-specific evoked fear response transfers to a new environment, mice were placed into a novel context and CS-dependent freezing was measured. RESULTS: Over 14 days, compared to controls, fear-conditioned pair-housed mice exhibited strong CS-evoked freezing in the homecage ( total freezing: FC 70.46%, CS-only 3.69%, F(2.29, 11.14)=10.78, p<0.01). Interestingly, when we evaluated CS-specific circadian responses, fear-conditioned mice exhibited enhanced freezing in the light versus the dark phase ( total freezing: light 84.56%, dark 56.36%, F(2.15, 6.46)=11.04, p<0.01). In fear-conditioned mice, there was a 36% reduction in total CS-dependent homecage freezing over 14 days. However, this was a context-dependent effect, as the reduction in freezing on day 14 was not observed in the novel context on day 15 ( t(3)=3.16, p=0.05). CONCLUSIONS: Here we demonstrate for the first time, using an open-source software developed in our laboratory, environment and circadian-dependent conditioned freezing responses in the homecage of group-housed mice. This study provides a proof-of-principle innovative approach for examining physiological outcomes of conditioned fear across ethologically relevant environments, which may help further the understanding for heightened physiological responses in neuropsychiatric diseases such as posttraumatic stress disorder (PTSD). To further elucidate impaired physiological mechanisms of disordered fear, future studies will integrate multimodal behavioral (DeepLabCut TM ) and cardiovascular telemetry-based approaches. Congressionally Directed Medical Research Programs (CDMRP) PR210574 This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

The contribution of probiotics for the double-edge effect of cefazolin on postoperative neurocognitive disorders by rebalancing the gut microbiota.

Emerging data suggest that perioperative gut dysbiosis is prevalent and may be associated with postoperative neurocognitive disorders (PND). Antibiotics and probiotics are key factors influencing the microbiota. Many antibiotics have anti-microorganisms and direct anti-inflammatory properties, which may have cognitive repercussions. NLRP3 inflammasome activation has been reported to be involved with cognitive deficits. This study aimed to determine the effect and mechanism of probiotics on neurocognitive problems associated with perioperative gut dysbiosis by the NLRP3 pathway. In a randomized, controlled trial, adult male Kunming mice undergoing surgery were administered cefazolin, FOS + probiotics, CY-09, or a placebo in four distinct experimental cohorts. Fear conditioning (FC) tests evaluate learning and memory. Following FC tests to evaluate inflammatory response (IR) and the permeability of barrier systems, the hippocampus and colon were extracted, and feces were collected for 16 s rRNA. One week after surgery, surgery/anesthesia decreased the frozen behavior. Cefazolin attenuated this declination but aggravated postoperative freezing behavior 3 weeks after surgery. Probiotics ameliorated surgery/anesthesia-induced memory deficits and perioperative cefazolin-induced postoperative memory deficits 3 weeks after surgery. NLRP3, caspase-1, Interleukin-1β (IL-1β), and Interleukin-18 (IL-18) levels were increased 1 week after the hippocampus and colon surgery, which were attenuated by CY-09 and probiotics, respectively. Probiotics could correct dysbacteria and IR caused by surgery/anesthesia stress and cefazolin alone. These findings imply that probiotics are an efficient and effective way of maintaining the balance of gut microbiota, which may reduce NLRP3-related inflammation and alleviate PND.

Cerebellar contribution to the regulation of defensive states.

Despite fine tuning voluntary movement as the most prominently studied function of the cerebellum, early human studies suggested cerebellar involvement emotion regulation. Since, the cerebellum has been associated with various mood and anxiety-related conditions. Research in animals provided evidence for cerebellar contributions to fear memory formation and extinction. Fear and anxiety can broadly be referred to as defensive states triggered by threat and characterized by multimodal adaptations such as behavioral and cardiac responses integrated into an intricately orchestrated defense reaction. This is mediated by an evolutionary conserved, highly interconnected network of defense-related structures with functional connections to the cerebellum. Projections from the deep cerebellar nucleus interpositus to the central amygdala interfere with retention of fear memory. Several studies uncovered tight functional connections between cerebellar deep nuclei and pyramis and the midbrain periaqueductal grey. Specifically, the fastigial nucleus sends direct projections to the ventrolateral PAG to mediate fear-evoked innate and learned freezing behavior. The cerebellum also regulates cardiovascular responses such as blood pressure and heart rate-effects dependent on connections with medullary cardiac regulatory structures. Because of the integrated, multimodal nature of defensive states, their adaptive regulation has to be highly dynamic to enable responding to a moving threatening stimulus. In this, predicting threat occurrence are crucial functions of calculating adequate responses. Based on its role in prediction error generation, its connectivity to limbic regions, and previous results on a role in fear learning, this review presents the cerebellum as a regulator of integrated cardio-behavioral defensive states.

Sex-specific effects of a parasite on stress-induced freezing behavior in a natural beetle-nematode system.

Some animals react to predation threats or other stressors by adopting a freezing posture in an attempt to avoid detection, and the duration of this behavior usually corresponds with individual personality, such that timid individuals freeze longer. Despite decades of research on this or related behaviors (thanatosis), never has the impact of parasitism been considered. Parasites could prolong the duration, if hosts are less motivated to move (i.e. lethargic), or they could reduce it, if hosts are motivated to forage more to compensate for energy drain. We examined this behavior within a natural beetle-nematode system, where hosts (horned passalus beetles, Odontotaenius disjunctus) are parasitized by a nematode, Chondronema passali. We exposed beetles (n = 238) to four stressors in our lab, including noise, vibration, light and inversion, and recorded how long they adopt a frozen stance. Afterward, we determined nematode burdens, which can range from dozens to hundreds of worms. Beetles tended to freeze for 20 seconds on average, with some variation between stressors. We detected no effect of beetle mass on the duration of freezing, and this behavior did not differ in beetles collected during the breeding or non-breeding season. There was a surprising sex-based difference in the impact of nematodes; unparasitized females remained frozen twice as long as unparasitized males, but for beetles with heavy nematode burdens, the opposite was true. From this we infer that heavily parasitized females are more bold, while males with heavy burdens would be more timid. The explanation for this finding remains elusive, though we can rule out many possibilities based on prior work on this host-parasite system.

Novel pathogenesis of post-traumatic stress disorder studied in transgenic mice

Posttraumatic stress disorder (PTSD) is very common after exposure to trauma, mental stress or violence. Because objective biological markers for PTSD are lacking, exactly diagnosing PTSD is a challenge for clinical psychologists. In-depth research on the pathogenesis of PTSD is a key for solving this problem. In this work, we used male Thy1-YFP transgenic mice, in which neurons are fluorescently labeled, to research the effects of PTSD on neurons in vivo. We initially discovered that pathological stress associated with PTSD increased the activation of glycogen synthesis kinase-beta (GSK-3β) in neurons and induced the translocation of the transcription factor forkhead box-class O3a (FoxO3a) from the cytoplasm to the nucleus, which decreased the expression of uncoupling protein 2 (UCP2) and increased mitochondrial production of reactive oxygen species (ROS) to trigger neuronal apoptosis in the prefrontal cortex (PFC). Furthermore, the PTSD model mice showed increased freezing and anxiety-like behaviors and more severe decrease of memory and exploratory behavior. Additionally, leptin attenuated neuronal apoptosis by increasing the phosphorylation of signal transducer and activator of transcription 3 (STAT3), which further elevated the expression of UCP2 and inhibited the mitochondrial production of ROS induced by PTSD, thus reducing neuronal apoptosis and ameliorating PTSD-related behaviors. Our study is expected to promote the exploration of PTSD-related pathogenesis in neural cells and the clinical effectiveness of leptin for PTSD.

Investigations on Rho/ROCK signaling in post-traumatic stress disorder-like behavior in mice

Post-Traumatic Stress Disorder (PTSD) is a chronic condition that occurs in response to a traumatic event, and consequently, enhances the threat sensitivity. Rho/ROCK signaling has been implicated in the consolidation of fear memory, stress, depression, anxiety, and traumatic brain injury. However, its role in post-traumatic stress disorder remains elusive. Therefore, the present study was designed to explore the role of fasudil, a Rho/ROCK inhibitor, a mouse model of PTSD. Mice were subjected to underwater trauma stress followed by three situational reminders. Underwater trauma (UWT) significantly increased the freezing behavior, a marker of the formation of aversive memory, in response to situational reminders on the 3rd, 7th, and 14th days, suggesting the significant development of PTSD. Trauma and situational reminders were also associated with significant changes in behavioral parameters in open field, social interaction and actophotometer tests, along with a reduction in serum corticosterone levels. Fasudil (10 and 15 mg/kg) and sertraline (15 mg/kg), a standard drug for PTSD, significantly decreased the freezing behaviour in response to situational reminders, suggesting the inhibition of the formation of aversive fear memory. However, fasudil and sertraline did not modulate normal memory functions, as assessed on elevated plus maze test, before subjecting mice to traumatic stress. Treatment with fasudil and sertraline significantly restored the behavioral changes and normalized the corticosterone levels. Fasudil-mediated blockade of the Rho/ROCK pathway may be responsible for blocking the formation of aversive memory during the traumatic event, which may be manifested in form of decreased contextual fear response during situational reminders.

Frozen mountain pine needles: The endodermis discriminates between the ice-containing central tissue and the ice-free fully functional mesophyll.

Conifer (Pinaceae) needles are the most frost-hardy leaves. During needle freezing, the exceptional leaf anatomy, where an endodermis separates the mesophyll from the vascular tissue, could have consequences for ice management and photosynthesis. The eco-physiological importance of needle freezing behaviour was evaluated based on the measured natural freezing strain at the alpine treeline. Ice localization and cellular responses to ice were investigated in mountain pine needles by cryo-microscopic techniques. Their consequences for photosynthetic activity were assessed by gas exchange measurements. The freezing response was related to the microchemistry of cell walls investigated by Raman microscopy. In frozen needles, ice was confined to the central vascular cylinder bordered by the endodermis. The endodermal cell walls were lignified. In the ice-free mesophyll, cells showed no freeze-dehydration and were found photosynthetically active. Mesophyll cells had lignified tangential cell walls, which adds rigidity. Ice barriers in mountain pine needles seem to be realised by a specific lignification patterning of cell walls. This, additionally, impedes freeze-dehydration of mesophyll cells and enables gas exchange of frozen needles. At the treeline, where freezing is a dominant environmental factor, the elaborate needle freezing pattern appears of ecological importance. This article is protected by copyright. All rights reserved.

Blockade of dopamine D3 receptor in ventral tegmental area attenuating contextual fear memory

The abnormal fear memory will lead to the onset of stress disorders, such as post-traumatic stress disorder (PTSD) and so on. Therefore, the intervention in the formation of abnormal fear memory will provide a new strategy for the prevention and treatment of PTSD. In our previous studies, we found that blockade of dopamine D3 receptor (DRD3) with highly selective antagonist YQA14 or knockout of DRD3 was able to attenuate the expression or retrieval of fear memory in PTSD animal models. However, the neurobiological mechanism of regulation of DRD3 in fear is unclear. In the present research, we clarified that DRD3 was expressed in the dopaminergic (DAergic) neurons in the ventral tegmental area (VTA). Then, we identified that microinjection of YQA14 (1 μg/0.2 μl/side) in VTA before the aversive stimuli in the training session or during days subsequent to the shock significantly meliorated the freezing behaviors in the inescapable electric foot-shock model. At last, using fiber photometry system, we found that microinjection of YQA14 in VTA promoted the dopamine neurotransmitter release in the basolateral amygdala (BLA), and pre-training YQA14 infusion in VTA lowered the increase of dopamine (DA) in BLA induced by shock during the training session or by context during the retrieval session. All above the results demonstrated that YQA14 attenuated the fear learning through the blockade of DRD3 in VTA decreasing the excitability of the projection to BLA. This study may provide new mechanisms and potential intervention targets for stress disorders with abnormal fear memory.

Competition between spin-glass and antiferromagnetic states in Tsai-type 1/1 and 2/1 quasicrystal approximants

Systematic research was performed to investigate magnetic properties of the Tsai-type $\mathrm{Ga}\text{\ensuremath{-}}\mathrm{Pd}\text{\ensuremath{-}}RE$ ($RE=\mathrm{Gd}$, Tb, Dy, and Ho) systems, where both 1/1 and 2/1 quasicrystal approximants (ACs) are attainable at the same compositions as thermodynamical stable phases. Most of the samples exhibited spin-glass (SG)-like freezing behavior at low temperatures except Ga-Pd-Tb 2/1 AC and Ga-Pd-Ho 1/1 AC. The former showcased antiferromagnetic order at 5.78 K while the latter did not show any anomaly down to 1.8 K. Furthermore, 2/1 ACs were noticed to be less frustrated than their corresponding 1/1 ACs (based on the empirical rule of $|{\ensuremath{\theta}}_{\mathrm{w}}/{T}_{\mathrm{f}}|$ ) presumably due to the disorder-free environment in the nearest neighbor of the rare-earth sites that form a network of distorted octahedron in the 2/1 ACs. The spin dynamic in SG samples was also characterized by means of ac magnetic susceptibility measurements. The results evidenced a weak response of the freezing temperatures to the measurement frequency in the Heisenberg systems, i.e., Gd-contained ACs, in contrast to the non-Heisenberg systems, i.e., Tb-, Dy-, and Ho-contained ACs, where significant dependency is noticed for the latter. The spin-glass samples were further examined by fitting their freezing temperatures to the Vogel-Fulcher law.

Control of Emotion and Wakefulness by Neurotensinergic Neurons in the Parabrachial Nucleus.

The parabrachial nucleus (PBN) integrates interoceptive and exteroceptive information to control various behavioral and physiological processes including breathing, emotion, and sleep/wake regulation through the neural circuits that connect to the forebrain and the brainstem. However, the precise identity and function of distinct PBN subpopulations are still largely unknown. Here, we leveraged molecular characterization, retrograde tracing, optogenetics, chemogenetics, and electrocortical recording approaches to identify a small subpopulation of neurotensin-expressing neurons in the PBN that largely project to the emotional control regions in the forebrain, rather than the medulla. Their activation induces freezing and anxiety-like behaviors, which in turn result in tachypnea. In addition, optogenetic and chemogenetic manipulations of these neurons revealed their function in promoting wakefulness and maintaining sleep architecture. We propose that these neurons comprise a PBN subpopulation with specific gene expression, connectivity, and function, which play essential roles in behavioral and physiological regulation.

Ferulic Acid Ameliorates Cell Injuries, Cognitive and Motor Impairments in Cuprizone-Induced Demyelination Model of Multiple Sclerosis.

Ferulic acid (FA) is a phenolic compound that exhibits neuroprotective effects in the central nervous system (CNS). This study was conducted to evaluate the potential effects of FA on the cognitive and motor impairments in the cuprizone-induced demyelination model of multiple sclerosis (MS). In this experimental study, demyelination was induced in mice by feeding them with chow containing cuprizone (CPZ) 0.2% for 6 weeks. Mice in the control group received normal chow. Mice in the CPZ+Veh, CPZ+FA10, and CPZ+FA100 groups received saline, and FA at a dose of 0, 10, or 100 mg/kg (intraperitoneal, I.P., daily) respectively. After cognitive and motor assessments, under anaesthesia, animal brains were removed for evaluating the histological, apoptosis, and molecular changes. The results showed that FA increased freezing behaviour in contextual (P<0.05) and cued freezing tests (P<0.05). FA also reduced the random arm entrance (P<0.01) and increased spontaneous alternations into the arms of Y-maze compared to the CPZ+Veh group (P<0.05). Time on the rotarod was improved in rats that received both doses of FA (P<0.01). Demyelination, apoptosis, and relative mRNA expression of p53 were lower in the FA-treated groups relative to the CPZ+Veh group (P<0.01). In addition, FA increased mRNA expression of brain-derived neurotrophic factor (Bdnf), Olig2, and Mbp (P<0.05) but decreased GFAP mRNA expression compared to the CPZ+Veh group (P<0.01). The results of this study showed that FA plays a significant neuroprotective role in CPZ models of demyelination by reducing neuronal apoptosis and improving oligodendrocytes (OLs) growth and differentiation.