Freeze-thaw Research Articles

Mesenchymal stem cell-derived protein extract induces periodontal regeneration: MSC-protein induces periodontal regeneration

BackgroundPeriodontal disease is characterized by chronic inflammation and destruction of supporting periodontal tissues, ultimately leading to tooth loss. In recent years, “cell-free treatment” without stem cell transplantation has attracted considerable attention for tissue regeneration. This study investigated the effects of extracts of mesenchymal stem cells (MSC-extract) and their protein components (MSC-protein) on the proliferation and migration of periodontal ligament (PDL) cells and whether MSC-protein can induce periodontal regeneration. MethodsMSC-extract and MSC-protein were obtained by subjecting mesenchymal stem cells (MSCs) to freeze–thaw cycles and acetone precipitation. Cell proliferation was examined using a WST-8 assay and Ki67 immunostaining, and cell migration was examined using Boyden chambers. The MSC-protein content was analyzed using liquid chromatography-mass spectrometry, protein arrays, and enzyme-linked immunosorbent assays (ELISAs). Gene expression in MSC-protein-treated PDL cells was examined using RNA-sequencing and Gene Ontology analyses. The regenerative potential of MSC-protein was examined using micro-computer tomography (CT) and histological analyses after transplantation into a rat periodontal defect model. ResultsMSC-extract and MSC-protein promoted the proliferation and migration of PDL cells. Protein array and ELISA revealed that MSC-protein contained high concentrations of basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Exogenous bFGF promoted the proliferation and migration of PDL cells. Furthermore, the transplantation of MSC-protein enhanced periodontal tissue regeneration with the formation of new alveolar bone and PDLs. ConclusionsThese results indicate that the MSC-protein promotes the proliferation and migration of PDL cells and induces significant periodontal tissue regeneration, suggesting that the MSC-protein could be used as a new cell-free treatment for periodontal disease.

Correction: Metal(loid)s Removal Response to the Seasonal Freeze–Thaw Cycle in Semi-passive Pilot Scale Bioreactors

Correction: Metal(loid)s Removal Response to the Seasonal Freeze–Thaw Cycle in Semi-passive Pilot Scale Bioreactors

Study on Freeze–Thaw Resistance of Basalt Fiber-Reinforced and Inorganic Curing Agent-Stabilized Shield Tunnel Muck

Study on Freeze–Thaw Resistance of Basalt Fiber-Reinforced and Inorganic Curing Agent-Stabilized Shield Tunnel Muck

Ultra-stretchable, robust, self-healable conductive hydrogels enabled by the synergistic effects of hydrogen bonds and ionic coordination bonds toward high-performance e-skins

Ionic conductive hydrogels as electronic skins (e-skins) have showcased pivotal potentials in the realm of human health monitoring and human–machine interfaces. However, developing intelligent hydrogel with remarkable stretchability and mechanical elasticity is yet challenging. Herein, high-performance ion-conducting hydrogels with unprecedented mechanical properties and good transparent, conductive, self-healing properties are constructed via free-radical polymerization of acrylic acid (AA) within the polyvinyl alcohol (PVA) network, and the subsequent freeze–thaw (F-T) treatment and Zr4+ ion immersion crosslinking technique. Profiting from the synergy of multiple intermolecular/intramolecular hydrogen bonds between different polymers and coordination bonds of carboxyl-Zr4+, the resultant PVA/PAA/Zr4+ hydrogel exhibits high transparency (∼97 %), superior conductivity (0.6089 S/m), a long elongation at break (2053 %), robust self-healing efficiency (96.3 %), high deformation-tolerate property, and notable mechanical repeatability. These multifaceted attributes render the hydrogel to serve as an ionic conductor for capacitive/resistive −type strain sensor. The hydrogel-based resistive-type strain sensor features a wide detection range (0.5 %-800 %), rapid response time (150 ms), long-term stability, and consistent output stability, making it promising in monitoring diverse human motions and seamless human–machine interactions. Additionally, the hydrogel sensor demonstrates excellent linear response in ultra-wide range (0–900 % strain), high sensitivity, and excellent cycling stability in a capacitive mode, enabling to be used for accurately detecting the weights of objects. As such, this work advances the development of ionic conductive hydrogel as flexible and wearable electronic devices.

Prediction of permanent deformation of subgrade soils under F-T cycles using SABO-optimized CNN-BiLSTM network

Accurately predicting the permanent deformation of subgrade soils under combined loading and seasonal freeze-thaw (F-T) effects is crucial for optimizing pavement design and maintenance planning in cold regions. However, existing empirical models have limitations in capturing the non-linear deformation behavior influenced by interacting factors. This study developed a deep learning framework, specifically a convolutional neural network-bidirectional long short-term memory (CNN-BiLSTM), for time-series prediction of subgrade strains. Laboratory tests established a comprehensive database to examine soil response across various conditions. Regression analysis revealed the constraints of traditional models. The optimized hybrid architecture directly learned patterns from experimental data, outperforming regression by 29−71 % based on error metrics. Sensitivity analysis confirmed that the model identified primary governing inputs consistent with theory. Hyperparameter tuning with the Subtraction-Average-Based Optimizer further enhanced performance. This data-driven methodology demonstrated the potential of advanced machine learning in simulating complex subsurface deformation behavior under compounding factors in seasonal frozen environments.

Effects of fly ash, activator ratio and steel fiber on freeze–thaw cycle, sulfuric acid, thermal conductivity and impact resistance of silica fume based AAHSMs

This study is focused on the effects of Class C fly ash (FA), NaOH/Na2SiO3 (SH/SS) ratio and steel fiber (STF) on the freeze–thaw (F-T) cycle, sulfuric acid, thermal conductivity and impact resistance of silica fume (SF) based alkali activated high strength mortars (AAHSMs). In the AAHSM mixtures, FA in different proportions (10 %, 15 %, 20 % and 25 %) is used instead of SF by weight. All the mixtures are prepared with water-to-binder rate of 0.32. F-T cycle results exhibit that the flexural strength (ffs) and compressive strength (fc) values of SF based AAHSMs without and with STF are less affected than those of ordinary Portland cement (OPC) based high strength mortars (OPCHSMs) without and with STF. After 300 F-T cycles, the ffs and fc values of SF based AAHSMs without and with STF are still over 14 MPa and 95 MPa, respectively. After exposure to 20 % sulfuric acid solution for 90 and 180 days, the SF based AAHSMs without and with STF exhibit super performance (i.e., fc values between 78.95 MPa and 96.81 MPa), while the surfaces of OPCHSMs without and with STF are disintegrated. Additionally, the effects of F-T cycle and sulfuric acid solution on SF based AAHSM and OPCHSM are examined with the microstructure analyses. Thermal conductivity results show that the SF based AAHSMs without and with STF perform better than the OPCHSMs without and with STF. The thermal conductivity results of OPCHSMs without and with STF vary between 0.630 W/m.K and 0.697 W/m.K, while the thermal conductivity results of SF based AAHSMs without and with STF range from 0.507 W/m.K and 0.623 W/m.K. Thermal conductivity results are adversely influenced by increasing STF content in the SF based AAHSMs and OPCHSMs. Impact resistance results show that the SF based AAHSMs without and with STF perform better than the OPCHSMs without and with STF. Moreover, the impact resistance values increase, as the amount of STF used in the SF based AAHSMs and OPCHSMs increases.

Material Design and Performance Study of a Porous Sound-Absorbing Sound Barrier

A new type of porous sound-absorbing sound barrier was developed with quartz sand and self-developed polysiloxane resin. The forming process of the material was studied. The test specimens of the porous sound-absorbing sound barrier were prepared with different mesh numbers of quartz sand and different proportions of resin, and the void properties, compressive strength, durability, and acoustic performance were investigated. Based on the mix design results, it is suggested that 20-mesh quartz sand and a 10:1 mass ratio of quartz sand are used to prepare the porous sound-absorbing sound barrier. The durability study showed that the porous sound-absorbing sound-barrier material had good salt and alkali resistance, poor acid resistance, good water stability, and freeze–thaw stability. The laboratory acoustic test and practical engineering application results showed that the porous sound-absorbing sound barrier had excellent acoustic performance and good noise-reduction effects.

Mechanical Properties, Durability Performance, and Microstructure of CaO-Fly Ash Solidified Sludge from Northeast, China.

In order to investigate the influence of the CaO and fly ash (FA) dosage and proportion on the mechanical properties, durability, and microstructure of solidified sludge, freeze-thaw (F-T) cycles and dry-wet (D-W) cycles are conducted to study the change in appearance and the strength attenuation of CaO-FA solidified sludge. Low-field nuclear magnetic resonance (LF-NMR) is used to analyze the microstructure of the solidified sludge with various dosages and ratios of CaO-FA. The results demonstrate that the unconfined compressive strength (UCS) and direct shear strength of solidified sludge increase with the prolongation of the curing age. Furthermore, the mechanical properties of solidified sludge are improved as the ratio of CaO-FA increases. As the curing age increases, the distribution of transverse relaxation time (T2) becomes narrow, the spectral area decreases, and the amplitude of the LF-NMR signal shows a downward and leftward tendency. Additionally, with the increase in the number of F-T cycles and D-W cycles, the UCS of solidified sludge declines and the degree of pore deterioration increased gradually. This study offers a theoretical foundation and empirical data for the dredging and treatment of sludge in cold regions.

Evidence from 162173 Ryugu for the influence of freeze–thaw on the hydration of asteroids

Evidence from 162173 Ryugu for the influence of freeze–thaw on the hydration of asteroids

Mechanically robust and highly conductive bacterial cellulose hydrogels through synergy of directional freeze–thawing and salting-out for wearable sensors

It is urgent to develop high-performance conductive hydrogels that simultaneously exhibit high biocompatibility, mechanical robustness, exceptional conductivity and frost resistance to fulfil the requirements of wearable flexible sensors. Herein, we present a simple and integrated approach for designing iso/anisotropic bacterial cellulose (BC) hydrogels by immersing the as-prepared polyvinyl alcohol (PVA)–BC–Borax hydrogels in saturated NaCl solutions using a salting-out effect to assist the non–/directional freeze–thawing strategy. Benefitting from the synergistic strengthening of molecular interactions, including hydrogen, coordination, and boron ester bonds, as well as the enhanced PVA and BC crystallinity resulting from the salting-out effect, the tensile strength (0.8–3.2 MPa) and high ionic conductivity (61.5–136.2 mS/cm) of isotropic hydrogels (PVA–BC–Borax/NaCl) can be well tuned by adjusting the feeding mass fractions of PVA, borax and BC. The optimised hydrogel, PVA7.5%–BC0.45%–Borax0.375%/NaCl, demonstrated remarkable mechanical properties (3.2 MPa, 295.1 %), ionic conductivity (72.6 mS/cm) and good anti-freezing properties (–40.8 °C), maintaining excellent conductivity (61.8 mS/cm) even at –20 °C due to the introduction of NaCl. When subjected to directional freeze–thawing cycles, the D(Directional)-PVA7.5%–BC0.45%–Borax0.375%/NaCl hydrogel, with its organised layers and larger pore–sized structures, exhibited higher breaking elongation (609.2 %) and ionic conductivity (81.6 mS/cm) than its isotropic counterpart (295.1 %, 72.6 mS/cm). The D-PVA7.5%–BC0.45%–Borax0.375%/NaCl hydrogel was successfully used as a strain sensor for detecting a variety of human movements, showing excellent responsiveness to external stimuli, thereby demonstrating its potential applications in human–machine interaction, flexible sensing and bioelectronics.

Investigating Mechanical Characteristics of Rocks Under Freeze–Thaw Cycles Using Grain-Based Model

AbstractBased on the discrete element method (DEM), a water-contained grain-based model (GBM) is developed in this study to evaluate the effects of freeze–thaw cycles (FTCs) on the mechanical characteristics of the rock. A set of freeze–thaw and uniaxial compression tests is carried out to explore the impact of micro damage caused by FTCs on the mechanical prosperities of rock samples. By monitoring the development and distribution of micro-cracks during freeze–thaw test and uniaxial compression test, the damage mechanism of FTCs is revealed from a microscopic perspective, which shows that FTCs deteriorate the strength and brittleness parameters as exponential functions. The parametric analysis is carried out to explore the influence of porosity and mineral components on the mechanical behaviors of rock against freeze–thaw and uniaxial loadings. Based on the parametric analysis results, it is found that UCS, Young’s modulus, and total strain energy at peak stress decrease with the increase of porosity and clay content, which emphasizes the contributions of porosity and mineral components on the mechanical properties of rock samples. It is proved that the water-contained grain-based model developed in this study can capture the damage caused by FTCs on the mechanical performance of rock from a microscopic perspective, which provides novel perspectives on the phenomenon of rock degradation in response to fluctuations in temperature.

Effects of sodium carboxymethyl cellulose-tea polyphenols ice coating on the quality degradation of frozen-thawed beef due to changes in protein structure and fat and protein oxidation

During freeze-thaw (FT) cycles, protein structural degradation, lipid and protein oxidation can lead to quality deterioration of beef samples. To address this issue, we developed a cost-effective and easy-to-operate carboxymethyl cellulose sodium-tea polyphenol (CMC-TP) ice coating to inhibit quality deterioration caused by these factors. The beef samples were characterized for various quality attributes, lipid and protein oxidation, and protein structure. The results demonstrated that the CMC-TP ice coating significantly inhibited the deterioration in water-holding capacity (WHC) and tenderness of the beef samples (P < 0.05). Analysis of peroxide value (POV), thiobarbituric acid (TBARS), total volatile basic nitrogen (TVB-N), and carbonyl content revealed that the CMC-TP ice coating significantly suppressed lipid and protein oxidation during FT cycles (P < 0.05). Additionally, assessments of total sulfhydryl content, fluorescence intensity, and surface hydrophobicity indicated that the CMC-TP ice coating effectively mitigated protein structural degradation through antioxidant and cryoprotective effects (P < 0.05). Therefore, the CMC-TP ice coating can enhance the FT stability of beef.

Triaxial creep test and damage model study of layered red sandstone under freeze-thaw cycles

To examine the creep behavior and degradation of stratified rock in cold areas, triaxial creep experiments and microscopic analyses were performed on layered red sandstone specimens under freeze-thaw (F-T) cycles. This study revealed the deterioration characteristics and damage mechanisms of red sandstone's creep behavior under the influence of F-T cycles and bedding inclination. The test results showed that: (1) The steady-state creep rates with bedding angles β=30°after 0, 40, 80, and 120F-T cycles were 0.0168 × 10−2·h−1, 0.0224 × 10−2·h−1, 0.0289 × 10−2·h−1, and 0.0368 × 10−2·h−1 respectively. The instantaneous deformation, creep deformation, and steady-state creep rates increased gradually with the increase of F-T cycles, while the long-term strength exhibited a decreasing trend. (2) After 40F-T cycles, the long-term strengths with bedding angles of 0°, 30°, 45°, 60° and 90° were 121.17 MPa, 65.47 MPa, 46.28 MPa, 77.64 MPa and 124.78 MPa, respectively. The bedding angle of 45° has the greatest influence on the triaxial creep properties of red sandstone, followed by the bedding angles of 30° and 60°, and the bedding angles of 0° and 90° have the least influence. (3) As the F-T cycles increases, the longitudinal wave velocity gradually decreases, and the pores and cracks on the sample surface continue to expand, leading to increased damage. The failure mode evolves from a single oblique shear plane to an “X”-shaped tensile splitting. Additionally, the bedding angle has a significant effect on the longitudinal wave velocity. As the bedding angle increases, the longitudinal wave velocity exhibits a “V”-shaped distribution, initially decreasing and then increasing. Based on the test results, a creep damage model of layered rock considering the influence of F-T cycles was established, and the rationality of the model was verified by test data. The research results can provide a scientific basis and technical reference for the long-term stability of layered rock engineering in cold regions.

Study on the weakening of mechanical properties and damage constitutive model of pre-cracked cyan sandstone after freeze–thaw cycles

Study on the weakening of mechanical properties and damage constitutive model of pre-cracked cyan sandstone after freeze–thaw cycles

Effects of freeze-thaw cycles on fatigue performance of asphalt mixture and a fatigue-freeze-thaw damage evolution model

To study the effect of freeze-thaw (FT) cycles on the fatigue performance of composite crumb rubber modified asphalt mixtures (CCRMA), a semicircular bending fatigue test based on the digital image correlation technique (DIC) was used to measure the fatigue life (Nf) of CCRMA and SBS modified asphalt mixtures (SBSMA) and the horizontal strain (Exx) of the DIC deformation field under repeated loading. The variation laws of Nf, Exx and the damage factor (D) defined based on Exx were analyzed under FT cycle conditions. In addition, considering the important influence of FT cycles on the Nf and damage evolution characteristics of asphalt mixtures, based on the classical Chaboche damage evolution model, the influence of FT cycle was considered, a fatigue-freeze-thaw damage evolution model that reflects the effects of FT cycling variables and stress levels on asphalt mixtures was constructed. The results indicate that the Exx peak of asphalt mixture shifts forward with increasing FT cycle number. After 20 FT cycles, the Nf of CCRMA decreased by 73.4 %, 62.1 %, and 75.1 %, while that of SBSMA decreased by 76.5 %, 58.6 %, and 74.6 % at stress ratios of 0.2, 0.3, and 0.4, respectively. The fatigue-freeze-thaw damage evolution model predictions were in agreement with experimental results, with the goodness of fit (R2) of all Nf prediction models being greater than 0.9. With the exception of a few outliers, the relative error between the measured and predicted Nf for the two asphalt mixtures was within 15 %. This indicates that the model can effectively predict the Nf and damage evolution laws of asphalt mixtures at different stress levels after an arbitrary number of FT cycles. The related research results provide a theoretical basis for predicting the Nf of new roads in cold regions under FT cycles.

Insights into the shallow landslide mechanism of expansive soil slope induced by freeze-thaw cycles and snowmelt infiltration

For rigorous understanding the shallow landslide mechanisms and deformation characteristics of expansive soil slopes, a comprehensive in-situ monitoring platform is established. Triaxial creep tests and microstructure analysis with scanning electron microscopy (SEM) are also conducted on expansive soil samples obtained from Binxi station. Field monitoring data indicates that freeze-thaw (F-T) cycle and snowmelt infiltration significantly increase the creep deformation of expansive soil slope during spring melting period. Due to the influence of F-T cycle and snowmelt infiltration, more soil grains are involved in the shear deformation contributing to a large, localized shearing. Additionally, the microstructural analysis shows that F-T cycle influence the relationship between expansive soil grains that gradually change from face-face contact to point-face contact or edge-edge contact form. The shallow landslide mechanisms of expansive soil slope are revealed from creep deformation and microstructure characteristics of soils after the F-T cycle and snowmelt infiltration, which can be summarized into two stages, namely, the snowfall accumulation state and snow melt-shallow infiltration stage. These results can serve as a good reference for the prevention of expansive soil slopes in seasonally frozen regions.

Performance of Asphalt Mixtures Modified with Desulfurized Rubber and Rock Asphalt Composites

This study explores the performance of asphalt mixtures modified with North American rock asphalt and desulfurized rubber particles at varying rubber-to-asphalt ratios ranging from 18% to 36% by weight. A comprehensive set of laboratory tests, including high-temperature rutting tests, low-temperature bending tests, indirect tensile tests, and freeze–thaw splitting tests, were conducted to evaluate the modified mixtures. The results indicate that both wet and dry blending methods produce mixtures that meet technical requirements, with the optimal asphalt-to-aggregate ratio determined to be 7.1%. At a rubber-to-asphalt ratio of 18%, the wet blending method slightly improves high-temperature rutting resistance compared to the dry method. However, an increase in rubber content generally enhances rutting resistance regardless of the blending technique. The wet blending method excels in low-temperature crack resistance, possibly due to better rubber dispersion, while an increase in rubber content diminishes crack resistance due to a thinning asphalt film. In terms of fatigue performance, the dry blending method results in significantly longer fatigue life, with a 27% rubber-to-asphalt ratio exhibiting optimal balance. The dry method consistently outperforms the wet method in water stability, and the resistance to water damage increases with rubber content. In conclusion, this study provides valuable insights into optimizing rubber-to-asphalt ratios and blending methods for various application needs, showcasing the benefits of rock asphalt and desulfurized rubber particles in asphalt modification.

Microwave self-healing characteristics of the internal voids in steel slag asphalt mixture subjected to salt-freeze-thaw cycles

Microwave heating technology can be used to repair freeze-thaw (F-T) cycle damage and cracks caused by the use of snow melting salts and de-icers in winter. This study utilized three different gradations of asphalt mixtures: AC-13, SMA-13, and OGFC-13, and enhanced their microwave absorption performance with steel slag (SS). The heating uniformity was evaluated through surface and internal temperature tests. Additionally, the mechanisms of damage and healing of the asphalt mixtures under salt freeze-thaw (S-F-T) damage conditions were explored using S-F-T cycles test and X-ray computed tomography scans (CT). The results showed that OGFC-13 exhibits a significantly higher rate of temperature increase compared to the other two types due to its stronger wave absorption capacity. The thermal hysteresis caused by steel slag leads to uneven spatial temperature distribution. The study recommends an optimal microwave heating time of 40 s for SS asphalt mixtures, which has minimal impact on the mixture structure, by defining an evaluation method for the uniformity index and local overheat ratio. After undergoing S-F-T cycles, the porosity of all three asphalt mixtures increased, particularly at the top and bottom of the specimens. Among them, OGFC-13 exhibited the poorest durability under S-F-T conditions, with the S-F-T cycles having a significant impact on medium-sized voids (0.15–0.6 mm). After microwave heating, AC-13 and SMA-13 showed good repair effects, with porosity levels returning to their initial states. For large voids (>2.36 mm) in OGFC, microwave heating may not effectively reduce the number of voids. Additionally, the healing rate of mixture specimens at certain mid-height ranges is higher due to elevated surface temperatures in these areas, making it especially effective in repairing F-T damage. This study shows that microwave heating of SS asphalt mixtures can improve the durability of pavement in cold regions. However, implementation challenges include the need for specialized equipment and potential uneven heating. Future research should explore optimizing microwave heating techniques to minimize local overheating and investigate the long-term performance of microwave-repaired asphalt pavements under various environmental conditions.

Effects of biochar and compost on the abundant and rare microbial communities assembly and multifunctionality in pesticide-contaminated soil under freeze‒thaw cycles

Biochar and compost are effective ways to improve soil quality and reduce pesticide pollution. However, the effects of them on the abundant and rare microbial communities in freeze‒thaw soil need to be further clarified. Therefore, this study took biochar, compost, and their combination as examples to explore their effects on the abundant and rare microbial communities and multifunctionality in glyphosate, imidacloprid and pyraclostrobin contaminated soil under freeze‒thaw cycles. We found that freeze‒thaw cycles enhanced the functional groups and surface aromaticity of biochar and compost, thereby improving the adsorption capacity. Biochar and compost reduced the concentration and half-life of three pesticides and enhanced the degradation function of rare taxa in soil. Biochar and compost improved the structure composition and co-occurrence relationship of abundant and rare taxa. Meanwhile, the assembly processes of abundant and rare sub-communities were mainly driven by stochastic processes and the Combined treatment promoted the transition from dispersal limitation to homogenizing dispersal and homogeneous selection. Moreover, the Combined treatment significantly improved the multifunctionality before and after freezing and thawing by increasing the diversity of rare taxa and assembly processes. The results provide new insights for farmland soil remediation in seasonal frozen areas, especially the soil functional cycle of abundant and rare microorganisms.

Study on Freeze–Thaw Resistance of Cement Concrete with Manufactured Sand Based on BP Neural Network

In this study, experiments were conducted on the freeze–thaw performance of manufactured sand cement concrete with different sand ratios and fly ash contents. The research found that during 200 freeze–thaw cycles, as the fly ash content increased, the concrete exhibited a higher mass loss rate and a decline in the relative dynamic modulus of elasticity. This was due to the lower activity of SiO2 and Al2O3 in the fly ash, which reduced the hydration products. Incorporating an optimal amount of manufactured sand can increase the density of concrete, thereby improving its resistance to freeze–thaw cycles. However, when the content of manufactured sand was high, its large surface area could interfere with the hydration process and reduce strength, thereby diminishing the freeze–thaw resistance of the concrete. Given that studying the freeze–thaw resistance of manufactured sand concrete is time-consuming and influenced by many factors, a prediction model based on a BP (back propagation) neural network was developed to estimate the mass loss rate and the relative dynamic modulus of elasticity following freeze–thaw cycles. After validation, the model was found to be highly reliable and could serve as a foundation for mix design decisions and freeze–thaw performance prediction of manufactured sand cement concrete.