Free State Province Research Articles

Household transmission of SARS-CoV-2 in a rural area in South Africa

Background. Patterns of SARS‐CoV‐2 spread have varied by geolocation, with differences in seroprevalence between urban and rural areas, and between waves. Household spread of SARS‐CoV‐2 is a known source of new COVID‐19 infections, with rural areas in sub‐Saharan Africa being more prone than urban areas to COVID‐19 transmission because of limited access to water in some areas, delayed health‐ seeking behaviour and poor access to care. Objectives. To explore SARS‐CoV‐2 infection incidence and transmission in rural households in South Africa (SA). Methods. We conducted a prospective household cluster investigation between 13 April and 21 July 2021 in the Matjhabeng subdistrict, a rural area in Free State Province, SA. Adults with SARS‐CoV‐2 confirmed by polymerase chain reaction (PCR) tests (index cases, ICs) and their household contacts (HCs) were enrolled. Household visits conducted at enrolment and on days 7, 14 and 28 included interviewer‐ administered questionnaires and respiratory and blood sample collection for SARS‐CoV‐2 PCR and SARS‐CoV‐2 immunoglobulin G serological testing, respectively. Co‐primary cases were HCs with a positive SARS‐CoV‐2 PCR test at enrolment. The incidence rate (IR), using the Poisson distribution, was HCs with a new positive PCR and/or serological test per 1 000 person‐days. Associations between outcomes and HC characteristics were adjusted for intra‐cluster correlation using robust standard errors. The secondary infection rate (SIR) was the proportion of new COVID‐19 infections among susceptible HCs. Results. Among 23 ICs and 83 HCs enrolled, 10 SARS‐CoV‐2 incident cases were identified, giving an IR of 5.8 per 1 000 person‐days (95% confidence interval (CI) 3.14 ‐ 11.95). Households with a co‐primary case had higher IRs than households without a co‐primary case (crude IR 14.16 v. 1.75, respectively; p=0.054). HIV infection, obesity and the presence of chronic conditions did not materially alter the crude IR. The SIR was 15.9% (95% CI 7.90 ‐ 29.32). Households with a lower household density (fewer household members per bedroom) had a higher IR (IR 9.58; 95% CI 4.67 ‐ 21.71) than households with a higher density (IR 3.06; 95% CI 1.00 ‐ 12.35). Conclusion. We found a high SARS‐CoV‐2 infection rate among HCs in a rural setting, with 48% of households having a co‐primary case at the time of enrolment. Households with co‐primary cases were associated with a higher seroprevalence and incidence of SARS‐CoV‐2. Sociodemographic and health characteristics were not associated with SARS‐CoV‐2 transmission in this study, and we did not identify any transmission risks inherent to a rural setting.

Challenges Encountered by Grade 10 Teachers in Implementing Selective Learning for Enhancing Learning Interest in Rural Schools in South Africa

Given the existing teaching and learning challenges in South Africa’s Basic Education system, selective learning will likely encounter implementation challenges as a method of enhancing effective learning interest in schools. Teachers have identified ineffectiveness and negative attitudes as some of the curriculum’s implementation challenges in the past and present. Against this background, this study explored the challenges that Grade 10 teachers encounter when implementing selective learning in schools. A qualitative research methodology was adopted for the study through the grounded theory approach. It utilized grounded theory as a research design and interpretive paradigm to guide the researcher. Data was collected using semistructured interviews as a data collection instrument. The sample consisted of eight teachers as participants from rural and urban high schools in the Mangaung District in Free State Province in South Africa. The study adopted purposive sampling techniques to select participants. Participants were qualified and experienced teachers with teaching qualifications and more than two years of teaching experience. The data was analysed using thematic analysis as a method of analysis. The thematic results revealed that teachers experience various challenges in implementing selective learning to enhance effective learning interest among Grade 10 learners. These include lack of adaptation, confusion, lack of teachers’ training, and shortage of resources. This study concludes that selective learning could be a helpful tool for enhancing compelling learning interest in South African schools regardless of the implementation challenges. Keywords: Selective learning, Learning Interest, Grade 10 Teachers, Shortage of Resources, Grade 10 Learners

Detection of Staphylococcus Isolates and Their Antimicrobial Resistance Profiles and Virulence Genes from Subclinical Mastitis Cattle Milk Using MALDI-TOF MS, PCR and Sequencing in Free State Province, South Africa.

Staphylococcus species are amongst the bacteria that cause bovine mastitis worldwide, whereby they produce a wide range of protein toxins, virulence factors, and antimicrobial-resistant properties which are enhancing the pathogenicity of these organisms. This study aimed to detect Staphylococcus spp. from the milk of cattle with subclinical mastitis using MALDI-TOF MS and 16S rRNA PCR as well as screening for antimicrobial resistance (AMR) and virulence genes. Our results uncovered that from 166 sampled cows, only 33.13% had subclinical mastitis after initial screening, while the quarter-level prevalence was 54%. Of the 50 cultured bacterial isolates, MALDI-TOF MS and 16S rRNA PCR assay and sequencing identified S. aureus as the dominant bacteria by 76%. Furthermore, an AMR susceptibility test showed that 86% of the isolates were resistant to penicillin, followed by ciprofloxacin (80%) and cefoxitin (52%). Antimicrobial resistance and virulence genes showed that 16% of the isolates carried the mecA gene, while 52% of the isolates carried the Lg G-binding region gene, followed by coa (42%), spa (40%), hla (38%), and hlb (38%), whereas sea and bap genes were detected in 10% and 2% of the isolates, respectively. The occurrence of virulence factors and antimicrobial resistance profiles highlights the need for appropriate strategies to control the spread of these pathogens.

Possible depression in new tuberculosis patients in the Free State province, South Africa.

Despite compelling evidence of comorbidity between tuberculosis (TB) and depression, little is known about the prevalence and determinants of depression among TB patients in the Free State province in South Africa. This study assessed the prevalence and factors associated with possible depression among new drug susceptible TB patients attending primary health care facilities. The study followed a cross-sectional design. Trained fieldworkers conducted face-to-face interviews with conveniently selected patients. Depression was assessed using the Patient Health Questionnaire-9. Data were subjected to descriptive and binomial logistic regression analyses. Out of 208 patients, 46.2% screened positive for possible depression - 22.6%, 18.8%, and 4.8% presenting with mild, moderate, and severe symptoms, respectively. Possible depression odds were three times higher among females than males (adjusted odds ratio [AOR]: 3.0; 95% confidence interval [CI]: 1.25-7.32) and 2.7 times higher among extrapulmonary TB (EPTB) than pulmonary TB patients (95% CI: 1.03-7.21). Longer TB treatment duration was protective (AOR: 0.8; 95% CI: 0.70-0.95) against depression. Among human immunodeficiency virus-positive patients, those on antiretroviral therapy (ART) had 2.5 times higher odds of depression (95% CI: 1.13-5.46) than those who were not. The results highlight a significant burden of possible depression among new TB patients, particularly among females, EPTB patients, and ART recipients. Longer TB treatment duration may offer some protection against depression symptoms, suggesting a need for enhanced adherence support. The results suggest that strengthening TB and mental health service integration is critical to improving treatment outcomes, overall well-being of TB patients, and the performance of the Free State TB programme.

A Retrospective Review of Neglected Tropical Diseases Diagnosed on Histopathological Specimens in the Free State Province, South Africa, 2015-2020.

Neglected tropical diseases (NTDs) are a heterogeneous group of medical conditions that commonly occur in underprivileged populations. NTDs are primarily diagnosed in tropical areas. Although South Africa is not situated in a tropical region, the high poverty rate makes the country susceptible to some NTDs. Limited data are available on the burden of NTDs in the Free State province of South Africa. This study aimed to determine the number of NTDs diagnosed on histopathological specimens in the public sector of the Free State province over a six-year period and to evaluate the patient demographics. A retrospective, descriptive study was performed. All NTDs diagnosed in histopathological specimens from public sector hospitals in the province submitted to the Department of Anatomical Pathology, National Health Laboratory Service, and University of the Free State between 1 January 2015 and 31 December 2020 were included in the study. The demographic information, biopsy site, and referring hospital were noted for each case identified. A total of 72 NTDs were diagnosed. The five most common diagnoses were echinococcosis (n = 33; 45.8%), bilharzia (n = 13; 18.1%), leprosy (n = 9; 12.5%), mycetoma (n = 8; 11.1%), and intestinal worms (n = 5; 6.9%). Ten (30.3%) patients diagnosed with echinococcosis came from the Free State's neighbouring country, Lesotho. Echinococcosis was the most prevalent NTD diagnosed in central South Africa. We recommend that the South African Department of Health add echinococcosis to the principal NTDs of significance in South Africa, alongside soil-transmitted helminths, schistosomiasis, leprosy, and rabies.

Wheat Stem Rust Surveillance Reveals Two New Races of Puccinia graminis f. sp. tritici in South Africa During 2016 to 2020.

Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is an important disease of wheat in South Africa (SA) and is primarily controlled using resistant cultivars. Understanding virulence diversity of Pgt is essential for successful breeding of resistant cultivars. Samples of infected wheat stems were collected across the major wheat-growing regions of SA from 2016 to 2020 to determine the pathogenic variability of Pgt isolates. Seven races were identified from 517 isolates pathotyped. The most frequently found races were 2SA104 (BPGSC + Sr9h,27,Kw) (35% frequency) and 2SA88 (TTKSF + Sr8b) (33%). Race 2SA42 (PTKSK + Sr8b), which was found in 2017, and 2SA5 (BFGSF + Sr9h), identified in 2017, are new races. The Ug99 variant race 2SA42 is similar in its virulence to 2SA107 (PTKST + Sr8b) except for avirulence to Sr24 and virulence to Sr8155B1. Race 2SA5 is closely related in its virulence to existing races that commonly infect triticale. Certain races showed limited geographical distribution. Races 2SA5, 2SA105, and 2SA108 were found only in the Western Cape, whereas 2SA107 and 2SA42 were detected only in the Free State province. The new and existing races were compared using microsatellite (SSR) marker analysis and their virulence on commercial cultivars was also determined. Seedling response of 113 wheat entries against the new races, using 2SA88, 2SA88+9h, 2SA106, and 2SA107 as controls, revealed 2SA107 as the most virulent (67 entries susceptible), followed by 2SA42 (64), 2SA106 (60), 2SA88+9h (59), 2SA88 (25), and 2SA5 (17). Thus, 2SA5 may not pose a significant threat to local wheat production. SSR genotyping revealed that 2SA5 is genetically distinct from all other SA Pgt races.

Capturing growth indices on the road to health booklets in clinics in Free State, South Africa.

Growth monitoring plays an essential role in the development of young children. Anthropometric indices are of utmost importance for healthcare professionals to identify children at risk of inadequate growth and malnutrition. This study aimed to assess the capturing of the growth indices in the Road to Health Booklets (RTHB) in clinics. The study was carried out in Mangaung Metropolitan municipal clinics in the Free State province, South Africa. A descriptive quantitative study was conducted using a checklist to audit 264 RTHBs. Descriptive statistics were used to analyse data. The findings showed that birth weight was recorded in most 99% (n = 262) of the RTHBs. The mid-upper arm circumference (MUAC) was not recorded in 58% (n = 153) of the cases during the last visit. Weight-for-Age (WfA) was routinely plotted in 91% (n = 241) of the RTHB. The length or Height-for-Age (LHfA) was plotted in 38% (n = 99) of the RTHB and Weight-for-Length or height (WfLH) was plotted in 31% (n = 81) of the RTHB. The results demonstrated that certain anthropometric measures including MUAC, length, or height were absent from the records of the RTHB. Consequently, RTHB may not be effectively used as a means of evaluating nutritional status, affecting early detection of malnutrition in children. The research makes a valuable addition to the existing body of knowledge for monitoring growth and measurement of anthropometric indices in the RTHB, as well as the appropriate execution of these practices.

First report of Phytophthora infestans lineage EU23 causing potato and tomato late blight in South Africa.

In South Africa, potato (Solanum tuberosum) late blight epidemics from 1996 to 2007 were caused by Phytophthora infestans clonal lineage US-1 (McLeod et al. 2001; Pule et al. 2013). Similarly, surveys on tomatoes in the mid-1990s only identified the US-1 clonal lineage in South Africa (McLeod et al., 2001). On potatoes, populations from the Southern Cape and Western Cape regions consisted of persistent mefenoxam-resistant populations (McLeod et al. 2001; Pule et al. 2013). Limited mefenoxam (R-enantiomer of metalaxyl) screening in 2021 in the Western Cape showed that potato isolates were sensitive, which prompted our study. Potato late blight samples were collected in 13 potato fields in the 2021 to 2023 seasons in the Western Cape (n = 4), Free State (n = 7), Limpopo (n = 1) and Kwazulu-Natal (n = 1) Provinces, and one tomato sample in 2022 in the Limpopo Province. Fourteen samples, one per field, were simple sequence repeat (SSR) genotyped for 12 loci (Li et al. 2013) using as DNA template, FTA cards, or genomic DNA extracted from cultures. P. infestans isolations from lesions and DNA culture extractions were conducted as previously described (Pule et al. 2013). SSR genotyping revealed that all 14 P. infestans samples belonged to clonal lineage EU_23_A1 (EU23), which has a phenotype (A1 and metalaxyl sensitive) and SSR genotype matching the US-23 lineage (Saville et al., 2021). As expected, minor polymorphisms were detected among the samples at loci Pi02, G11, D13 and SSR4. Mefenoxam sensitivity testing of seven potato isolates from the Free State (n = 3) and Western Cape (n = 4), and one tomato isolate was conducted as previously described (Mcleod et al. 2001). All isolates were sensitive to mefenoxam since no infection and sporulation occurred at 3 µg/ml. This was expected since EU23 has been reported as mefenoxam sensitive in other countries (Kawchuk et al., 2011; McGrath et al., 2015). Replacement of the US-1 clonal lineage by EU23 suggests that the latter lineage is more aggressive or fit than US-1, but this must be verified especially on potatoes. On tomatoes, on the other hand, EU23 is known as a highly aggressive lineage (Kawchuk et al., 2011; McGrath et al., 2015; Saville et al., 2021). Therefore, population displacements may have first occurred on tomatoes from where the lineage spread to potatoes. In the Cape coastal potato production regions, population displacement may have been supported by the withdrawal of mefenoxam/metalaxyl from the region since 1996 because the EU23 lineage is mefenoxam sensitive, as opposed to the previously prevailing US-1 mefenoxam-resistant lineage. More severe potato late blight epidemics has not been observed in recent years in South Africa. However, tomato late blight has increased and is more prevalent in the Limpopo province. The source of the introduction of EU23 into South Africa is unknown. Only test-tube plants and/or greenhouse tubers may be imported into South Africa since 1997. Therefore, the illegal importation of planting material may have introduced the new genotype. Whether this could have occurred from neighbouring African countries is unknown since P. infestans genotyping has not been conducted in these countries. In Africa, EU23 has been reported in northern African countries (Tunisia, Algeria and Egypt) (Saville et al., 2021; El-Ganainy et al., 2023). Mefenoxam and metalaxyl applications will likely be effective again in the Western Cape, but more samples will have to be tested to confirm this. This will provide growers with a more cost-effective fungicide (metalaxyl) since alternative actives with comparable systemic and curative activity are more expensive.

In Vitro Bioactivities of Plants Used against Skin Diseases in the Eastern Free State, South Africa

Skin diseases are a worldwide issue, accounting for approximately 34% of all occupational illnesses. The aim of this study was to investigate medicinal plants used to treat wounds and skin diseases in the eastern Free State Province of South Africa. An ethnobotanical survey was conducted to gather information from traditional healers on plants they use to treat human ailments. Plants were collected and then investigated for antimicrobial, antioxidant and anti-inflammatory properties using standard assays. Cotyledon orbiculata, Dioscorea sylvatica, and Lycopodium clavatum had the highest frequency of citation (RFC) values among the 22 plants reported. Saponins, flavonoids, terpenoids, alkaloids, anthraquinones, and tannins were found in the phytochemical examination. L. clavatum had the greatest activity against Staphylococcus aureus and Pseudomonas aeruginosa at 0.39 mg/mL and 0.098 mg/mL, respectively. C. orbiculata and D. sylvatica extracts showed significant antifungal activity between 0.39 and 1.56 mg/mL. Antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl radicals was found in all extracts. The extracts had significant anti-inflammatory action against the 5-lipoxygenase enzyme, with IC50 values ranging from 0.02 to 0.49 g/mL. The usage of C. orbiculata, D. sylvatica, and L. clavatum in the treatment of skin problems in the Eastern Free State of South Africa was verified in this research.

Challenges in promoting accountability and feedback mechanisms through customer complaints in the South African public service

The study examines whether the customer complaints management policy in the Department of Employment and Labour in the Free State province is effectively and efficiently implemented. Skilful and committed policy implementers are required to ensure that policies are implemented successfully. A descriptive qualitative research design was employed in this study using semi-structured online interviews, to gather data from 20 participants who were purposively selected. The major findings of the study reveals that a lack of clarity and accessibility of complaint channels, inadequate training of newly appointed officials in customer complaint management processes, and user-unfriendliness of the complaint processes flow are prohibiting factors of the implementation of the policy. Additionally, there was a lack of effective communication and coordination among both the internal and external stakeholders, and a noticeable absence of consequence management. The study recommend that the Department of Employment and Labour should consider to design a user-friendly customer complaints management processes flow as well as to incorporate customer complaints module during the induction of newly appointed employees.

“I Mainly Rely on the Textbook:” A Call for Teacher Enhancement in Agricultural Sciences

Universities play a crucial role in teachers' professional development. This study aims to examine the following research question: How can universities enhance the teaching of agricultural sciences in schools? Previous research has focused on the university’s collaboration with stakeholders, such as parents, to enhance teaching, but without a specific focus on the teaching of agricultural sciences. In response to this question, a transformative theoretical framework anchors this study. This study employed a participatory action research design involving focus group discussions to determine the need for improving the teaching of agricultural sciences. Eleven participants were involved in this research, and pseudonyms were used to ensure privacy and protect their identities. The study findings from the three schools in the Thabo Mofutsanyana District of the Free State Province, included in the study, revealed that there is an inability to meet curriculum requirements, accompanied by a reluctance to implement continuous professional teacher development. The findings emphasize the need for extended university involvement in the implementation of teacher development policies to address the teachers’ needs.

Local language proficiency of fourth-year medical students at the University of the Free State.

Language proficiency is beneficial for doctor-patient communication and health outcomes. Poor communication can lead to misdiagnosis by the doctor and/or non-adherence from the patient. This study aimed to evaluate medical students' proficiency in the most commonly spoken local languages. This cross-sectional study was conducted in the class of 119 fourth-year medical students at the University of the Free State (UFS) in 2019. Students' proficiency was tested for Sesotho and Afrikaans, as these are the most widely spoken languages in the Free State province. The study consisted of two phases: completing a self-administered questionnaire where students self-rated their proficiency in the two languages, followed by telephonic interviews consisting of a series of proficiency-testing questions. Of the 119 fourth-year medical students at UFS, 96 (80.7%) completed the self-administered questionnaires. Forty-six students (47.9%) rated themselves as either advanced or proficient in Afrikaans, whereas only 23 students (23.9%) rated themselves as advanced or proficient in Sesotho. Only 28 students were subsequently interviewed. Their actual language proficiency matched their self-rating. The findings suggest a need for language skills training improvement in the curriculum for undergraduate medical students for languages most commonly encountered locally. We also found that students report their language capabilities accurately.Contribution:The research findings reinforce the need for language skills training in the curriculum of undergraduate medical students regarding languages commonly encountered in the local area.

Designing effective U = U communication strategies considering the needs of PLHIV, their partners, and healthcare worker constraints in South African clinics.

We sought to understand the Undetectable = Untransmittable (U = U) communication needs of persons living with HIV (PLHIV) and barriers to U = U communication among healthcare providers (HCPs) in South Africa. We conducted five focus group discussions (FGDs) with HCPs (N = 42) including nurses and counsellors from primary healthcare clinics (PHCs) in the Gauteng and Free State Provinces of South Africa, three FGDs (N = 27) with PLHIV recruited by snowball sampling from civil society organizations, and 27 in-depth interviews (IDIs) with recently diagnosed PLHIV in Johannesburg. IDIs and FGDs were audio recorded, transcribed, translated to English, and analysed thematically. PLHIV were largely unaware and sceptical of U = U as the message appeared to contradict the mainstream HIV prevention clinical guidance. The low viral load (VL) knowledge further reduced confidence in U = U. PLHIV need support and guidance on the best approaches for sharing U = U information and disclosing their VL status to their partners, highlighting the central role of community understanding of U = U and VL to mediate the desired stigma reduction, social acceptance and emotional benefits of U = U for PLHIV. HCPs were uneasy about sharing U = U due to concerns about risk compensation and ART non-adherence and worried about enabling any ensuing HIV transmission. HCPs also need a simple, unambiguous, and consistent narrative for U = U, integrated with other HIV prevention messages. PLHIV and HCPs alike recommended a patient-centred approach to communicating U = U, focusing primarily on attaining viral suppression and emphasizing that condomless sex is only safe during periods of ART adherence. These data highlight the need for simple U = U communication support targeting both HCP and PLHIV. Culturally appropriate communication materials, with training and ongoing mentorship of the clinic staff, are essential to improve patient-centred U = U communication in clinics.

Enhancing the Support System by the Department of Education to Teachers in Rural Setting of South Africa

This study examines the support system offered by the Department of Education in South African schools in the Free State Province of South Africa Participants were a purposive sample of t eight (8) teachers in rural setting of South Africa (female = 80%, 26 to 55 years old). Data on the support system by the Department of Education to support teachers were collected by means of semi-structured individual interviews. The data were thematically analysed by means of open coding. The study indicated that it is thought that improving the overall effectiveness of the educational system requires increasing the quality of teacher performance through teacher development programs. The study further indicated that viewed support in terms of teacher development especially on issues of instructional leadership or leadership in a general sense, curriculum delivery, while others viewed support in terms of resource provision. When it comes to resource provision, they expected to be provided with materials that would help them to execute their duties successfully. The study indicated that the support that is to be provided by the District Support Team has to be informed by teacher’s needs, meaning there must be a collaboration between the two stakeholders whereby they sit and plan together on issues that are of great concern regarding the context of the school.

The role of drylines in thunderstorm initiation and record rainfall during an anomalously convective early summer season in South Africa

An average of 12 drylines are associated with thunderstorm development (convective drylines) during December in the western plateau of subtropical southern Africa. During December 2021, 20 convective drylines occurred. This was the highest number since the record began in 2010. December 2021 was also unusually wet over a large part of the summer rainfall region in South Africa. Monthly rainfall records were broken in the Northern Cape, Northwest and Free State provinces. Above-average rainfall had dire consequences for the maize industry over the central interior. The large-scale circulation during December 2021 was very conducive to dryline formation over the western plateau with a deeper than usual surface trough and strong low-level northeasterly moisture flux towards the climatologically dry western plateau region. Above normal wind speeds of mid-tropospheric northwesterlies and large-scale uplift contributed to a favorable environment for strong thunderstorms, as seen during a 6-day convective dryline event. Thunderstorms which were triggered along the dryline travelled east and contributed to the above normal rainfall observed during December 2021. Dryline and rainfall frequencies show large inter-annual variability. However, a positive Pearson correlation (r = 0.45; p < 0.05) between the number of dryline days and rainfall amounts were found between 1979 and 2021. This correlation increased (r = 0.53; p < 0.05) when rainfall amounts immediately east of the western plateau domain were also included, suggesting that dryline-related convection can have a significant influence on rainfall over the western plateau and further downstream.

Exploring Negative Teacher Behaviour towards Learners: The Long-Term Psycho-Emotional Effects

Abstract The importance of teachers in a child’s development is well known. However, relatively little research has been conducted on their influence on a child’s psycho-emotional development, and almost none on long-term negative effects. This study aimed to add to the much-needed data in this regard. It focused on 115 respondents in Free State province, South Africa. The respondents consisted of primary and high school teachers, ranging in age from 20 to older than 65 (sd = 10.84). More female (n = 81, 70%) than male (n = 34, 30%) respondents participated, and most were white (n = 92, 80%). A mixed-methods research design was employed, making use of a self-compiled questionnaire. More than 50% of the respondents indicated that they continue to experience psycho-emotional impacts such as depression and feelings of inferiority inflicted by teachers many years prior. Limitations of the study are discussed, and recommendations are made on how to address this important problem.

Enhancing visual-motor integration and visual perception of 6-year-old children

Background: Perceptual motor development is crucial during early childhood and not properly addressing it in physical education (PE) can be detrimental.Aim: To determine the effect of a South African curriculum-aligned PE intervention on the visual-motor integration (VMI), visual perception (VP) and motor coordination (MC) of 6-year-old children.Setting: Quintile 3 schools in Mangaung, Free State Province, South Africa.Methods: Grade 1 children from two quintile 3 schools in Mangaung were recruited. Complete data sets were obtained for 44 participants. A quantitative randomised control trial design was followed. The Beery-Buktenica Developmental Test of Visual-Motor Integration, Sixth Edition (Beery VMI-6), determined participants’ VMI, VP and MC during the pre- and post-tests. The KaziKidz toolkit was used as intervention during 10 sessions of 40 min each for the experimental group, while the control group continued with the South African PE curriculum. The Fisher’s exact and Kruskal–Wallis tests were used for pre- and post-test comparisons, with p 0.05 indicating statistical significance.Results: The median age of the control (n = 18) and intervention (n = 26) groups was 6.7 and 6.5 years, respectively. Post intervention, the intervention group displayed significantly improved VMI (p = 0.042) and VP (p 0.001), compared to the control group. No significant differences between the groups were observed for MC.Conclusion: Exposure to PE including perceptual motor aspects significantly improved the VMI and VP of 6-year-old children.Contribution: Results deemed the KaziKidz toolkit to be a successful mode of PE delivery to improve the perceptual skills of Grade 1 learners in South Africa.

Three novel sequencing types from seventeen Staphylococcus aureus genomes isolated from dairy cows milk in the Free State Province of South Africa.

Staphylococcus aureus is one of the major pathogens causing bovine mastitis, which results in huge economic losses in the dairy industry worldwide. Here, we report genome sequences of 17 S. aureus strains, with three novel sequencing types (ST8495, ST8500, and ST8501) isolated from the milk of dairy cows with subclinical mastitis.

Impact of COVID-19 on selected essential public health services – lessons learned from a retrospective record review in the Free State, South Africa

BackgroundIn an attempt to discern lessons to improve future pandemic responses, this study measured the effects of the COVID-19 pandemic on essential public health services (EPHSs) related to primary health care (PHC) and outpatient department (OPD) utilisation, antiretroviral treatment (ART) commencement, drug-susceptible tuberculosis (DS-TB) confirmation and treatment commencement, and Bacillus Calmette-Guérin (BCG) coverage, in the Free State province of South Africa during January 2019 to March 2021.MethodsA pre-post study design comparing EPHS performance between 2019 and 2020/21 was employed. Routinely collected data were analysed. An interrupted time series analysis was used to measure changes in service use and outcomes from January 2019 to March 2021. Median changes were compared using Wilcoxon rank-sum tests. A 5% statistical significance level was considered.ResultsOver the study period, the median values for the annual number of PHC visits was 1.80, 55.30% for non-referred OPD visits, 69.40% for ART commencement, 95.10% and 18.70% for DS-TB confirmation and treatment commencement respectively, and 93.70% for BCG coverage. While BCG coverage increased by 5.85% (p = 0.010), significant declines were observed in PHC utilisation (10.53%; p = 0.001), non-referred OPD visits (12.05%; p < 0.001), and ART commencement (9.53%; p = 0.017) rates. Given the importance of PHC in addressing a new pandemic, along with the existing HIV and TB epidemics – as well as the entire quadruple burden of disease – in South Africa, the finding that the PHC utilisation rate statistically significantly decreased in the Free State post-COVID-19 commencement is particularly concerning.ConclusionsThe lessons learned from this retrospective review attest to a measure of resilience in EPHS delivery in the Free State in as far as a significant hike in BCG vaccination over the study period, 2019–2020/21 was observed. As evidenced by a decline in PHC service utilisation and the decreased numbers of new patients commencing ART, we also learned that EPHS delivery in the province was fragile.

Spatiotemporal analysis of heavy summer rainfall events across the Free State Province, South Africa: 1981–2022

ABSTRACT Heavy rainfall events negatively impact agricultural productivity (e.g. crop damage) and human health (e.g. waterborne disease outbreaks). Under anthropogenically induced warming, South Africa may experience more frequent and heavier rainfall events during the summer wet-season. Evidence from point-based rainfall records suggests that such changes may already be occurring across many summer rainfall zone regions; however, the understanding thereof is spatially unresolved. Therefore, focusing on the summer rainfall zone Free State Province, we used the CHIRPS rainfall dataset for 1981/82–2021/22 to explore trends and interannual variability in the magnitude and frequency of heavy rainfall events for summer months (October–April). Interannually, the El Niño-Southern Oscillation had a predictable influence, with positive (negative) anomalies for the frequencies and magnitudes of heavy rainfall events during La Niña (El Niño) years. Despite few statistically significant trends, the results broadly depict increases (decreases) by up to 0.4 days/decade (−0.2 days/decade) in the frequency of smaller (larger) heavy rainfall events. More consistent trend results were evident for heavy rainfall event magnitude indices, with declining (increasing) trends, up to −2 mm/decade (5 mm/decade), evident over central (northeastern and southwestern) regions. Our results highlight regions vulnerable to changes and interannual variations in heavy rainfall characteristics, which can inform adaptation strategies.