Oak bark, which is commonly used in the wood industry, has by-products often repurposed as fuel. Its extracts are rich in compounds with anticancer, antibacterial, antifungal, and anti-inflammatory properties. This study synthesized platinum nanoparticles (PtNPs) using aqueous extracts from Quercus dalechampii (QD), Q. frainetto (QF), and Q. petraea (QP). Key factors during nanoparticle formation included reaction time, metal ion concentration, pH, extract-to-metal ion ratio, and temperature. The PtNPs were characterized by dynamic light scattering, Fourier transform infrared spectroscopy, and transmission electron microscopy. The average diameters were 58.5 ± 7.6 nm for QD-PtNPs, 41.6 ± 5.4 nm for QF-PtNPs, and 41 ± 5.3 nm for QP-PtNPs. Antioxidant and antimicrobial activities were also analyzed. The QP-PtNPs had the highest DPPH (2,2-Diphenyl-1-picrylhydrazyl), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (Cupric Reducing Antioxidant Capacity) free radical scavenging activities, while QD-PtNPs excelled in ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) scavenging. All PtNPs showed strong antimicrobial properties, particularly against Enterococcus faecalis, Escherichia coli, Candida krusei, and Candida auris. These findings suggest that Quercus-mediated PtNPs have significant potential for developing treatments against bacterial and fungal infections, with promising applications in medicine.