The effects of in-medium nucleon-nucleon cross sections on the stopping observable and ratio of free protons in heavy-ion collisions at 400MeV/nucleon have been investigated within the framework of the IQMD+GEMINI model. Five kinds of in-medium corrections of nucleon-nucleon cross sections, which are considerably different in the referred energy and density, have been used in the model. It has been found that calculations of the stopping decrease when the in-medium cross sections decrease. Moreover, the ratio of free protons Rp depends not only on the value of the in-medium factors but also on its isospin dependence. In order to investigate the isospin effect of in-medium factors on the ratio of free protons Rp, the isospin dependence of in-medium factors has been adjusted and used in the model. The calculations have shown that the isospin dependence of in-medium factors does not impact the stopping, but impacts the ratio of free protons Rp. When the in-medium factors relation f nn med > f pp med is used in the model, the calculated values of Rp are larger than those in the f nn med < f pp med case.
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