In this paper, we investigate relations or differences among various conserved quantities which involve the matter Energy Momentum Tensor (EMT) in general relativity. These quantities include the energy with Einstein’s pseudo EMT, the generalized Komar integral, or the ADM energy, all of which can be derived from Noether’s second theorem, as well as an extra conserved charge recently proposed in general relativity. For detailed analyses, we apply definitions of these charges to a system of free massive particles. We employ the post-Newtonian (PN) expansion to make physical interpretations. We find that the generalized Komar integral is not conserved at the first non-trivial order in the PN expansion due to non-zero contributions at spatial boundaries, while the energy with Einstein’s pseudo EMT at this order agrees with a total energy of massive particles with gravitational interactions through the Newtonian potential, and thus is conserved. In addition, this total energy is shown to be identical to the ADM energy not only at this order but also all orders in the PN expansion. We next calculate an extra conserved charge for the system of massive particles, at all orders in the PN expansion, which turns out to be a total number of particles. We call it a gravitational charge, since it is clearly different from the total energy. We finally discuss an implication from a fact that there exist two conserved quantities, energy and gravitational charge, in general relativity.