The interaction of the free nucleic acid bases adenine, cytosine, and thymidine with meso-tri(4-sulfonatophenyl) meso 4-phenyl porphyrin cytosine amide (TPSC) trisodium salt at pH 5.9, 7.4, and 9.3 results is a decrease in the absorbance of the Soret (B-band) at 413 nm of TPSC. A decrease in Soret band absorbance is observed with guanine below pH 8; at higher pH values a new absorbance band at 424 nm is observed. The appearance of the band is consistent with hydrogen bonding between the cytosine-functionalized porphyrin and guanine and the resulting perturbation of the electron orbitals of the porphyrin.