Abstract
AbstractA method of liquid chromatography was applied to the analysis offree nucleic acid bases in photographic gelatins. It was possible to analyse free nucleic acid bases in photographic gelatins without any pre-treatment of the sample gelatin solution.Highly restraining gelatins contained free nucleic acid bases, while low restraining or inert gelatins contained little of them. These free nucleic acid bases in gelatins were eliminated by an ion-exchange treatment. The pH dependence of physical retardance was changed by the treatment. The effect of free nucleic acid bases on the physical retardance was proved.
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