Abstract
AbstractA high-performance liquid chromatographic method was applied to the analysis of nucleic acid bases in photographic gelatins by a cation exchange resin. The sample photographic gelatin was hydrolyzed with hydrochloric acid. The contents of nucleic acid bases were calculated quantitatively from their calibration curves and the chromatograms of photographic gelatins. Highly restraining gelatins contained a relatively large amount of these bases, while lowly restraining or inert gelatins contained little of them. In highly restraining gelatins, the ratio of adenine to guanine was approximately 1: 1. It shows that highly restraining gelatins contained not only nucleic acid but also free nucleic acid bases and so on.
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