Free Lipids Research Articles

Oxygen Exposure and Tolerance Shapes the Cell Wall-Associated Lipids of the Skin Commensal Cutibacterium acnes.

Cutibacterium acnes is one of the most abundant bacteria on the skin. Being exposed to oxygen and oxic stress, the secretion of the bacterial antioxidant protein RoxP ensures an endogenous antioxidant system for the preservation of skin health. To investigate the impact of the antioxidant RoxP on oxidation of the bacteria, wildtype and an isogenic roxp mutant were cultured in anaerobic and oxic conditions. The carbonylated status of proteins were recorded, as were the most significant modifications in a relative intensity of free fatty acids (FFA) and lipids containing fatty acids (FA), such as di- (DG) and triglycerides (TG), di- (DGDG) and sulfoquinozyldiacylglycerol (SQDG) and ceramides. Concerning the fatty acid types, it was observed that the free fatty acids contained mainly C12:0-C26:0 in hydroxy and acylated forms, the DG contained mainly C29:0-C37:0, the TG contained mainly C19:0-C33:0, and the DGDG/SQDGs contained very long fatty acids (C29:0-C37:0) demonstrating the interdependence of de novo synthesis of lipids and RoxP. The area of DGDG peaks (924.52, 929.56 and 930.58) were affected by bacterial growth conditions, with the exception of m/z 910.61. Moreover, the FFA unsaturation is wider in the SQDG species (C30:0 to C36:6) than in DG, TG or free FFA species. It could be concluded that both environmental oxidative statuses, as well as the prevalence of bacterial antioxidant systems, significantly shape the lipidome of C. acnes.

Dihydromyricetin supplementation improves ethanol-induced lipid accumulation and inflammation.

Excessive alcohol consumption leads to a myriad of detrimental health effects, including alcohol-associated liver disease (ALD). Unfortunately, no available treatments exist to combat the progression of ALD beyond corticosteroid administration and/or liver transplants. Dihydromyricetin (DHM) is a bioactive polyphenol and flavonoid that has traditionally been used in Chinese herbal medicine for its robust antioxidant and anti-inflammatory properties. It is derived from many plants, including Hovenia dulcis and is found as the active ingredient in a variety of popular hangover remedies. Investigations utilizing DHM have demonstrated its ability to alleviate ethanol-induced disruptions in mitochondrial and lipid metabolism, while demonstrating hepatoprotective activity. Female c57BL/6J mice (n = 12/group) were treated using the Lieber DeCarli forced-drinking and ethanol (EtOH) containing liquid diet, for 5 weeks. Mice were randomly divided into three groups: (1) No-EtOH, (2) EtOH [5% (v/v)], and (3) EtOH [5% (v/v)] + DHM (6 mg/mL). Mice were exposed to ethanol for 2 weeks to ensure the development of ALD pathology prior to receiving dihydromyricetin supplementation. Statistical analysis included one-way ANOVA along with Bonferroni multiple comparison tests, where p ≤ 0.05 was considered statistically significant. Dihydromyricetin administration significantly improved aminotransferase levels (AST/ALT) and reduced levels of circulating lipids including LDL/VLDL, total cholesterol (free cholesterol), and triglycerides. DHM demonstrated enhanced lipid clearance by way of increased lipophagy activity, shown as the increased interaction and colocalization of p62/SQSTM-1, LC3B, and PLIN-1 proteins. DHM-fed mice had increased hepatocyte-to-hepatocyte lipid droplet (LD) heterogeneity, suggesting increased neutralization and sequestration of free lipids into LDs. DHM administration significantly reduced prominent pro-inflammatory cytokines commonly associated with ALD pathology such as TNF-α, IL-6, and IL-17. Dihydromyricetin is commercially available as a dietary supplement. The results of this proof-of-concept study demonstrate its potential utility and functionality as a cost-effective and safe candidate to combat inflammation and the progression of ALD pathology.

Hair Lipid Structure: Effect of Surfactants

Human hair fibres are mainly comprised of proteins (>90%) and lipids (1–9%), which are characterised as exogenous or endogenous, depending on whether they originate from sebaceous glands or hair matrix cells, respectively. Exogenous lipids consist of free fatty acids (FFAs), triglycerides, cholesterol (CH), wax esters, and squalene. Endogenous hair lipids comprise FFAs, CH, ceramides, glycosylceramides, cholesterol sulfate, and 18-methyleicosanoic acid. Lipids were demonstrated to be fundamental against damage and maintenance of healthy hair. Several studies have evaluated the effects of hair lipid content and have shown how hair properties were altered when lipids were removed by solvent extraction. The effect of surfactants on hair lipids is difficult to determine, as the complex structure of the cell membrane complex makes it difficult to determine where surfactants act. Shampoos and conditioners contain surfactants that remove lipids during routine cleansing of hair. However, shampooing does not completely remove all free lipids from the surface layers. The effect of surfactants on the alteration and removal of structural lipids is poorly developed, and there is no consensus on the results. Further research on the lipid composition of the hair could provide information on the penetration pathways of surfactants to improve effectiveness and limit possible damage.

Orchestral role of lipid metabolic reprogramming in T-cell malignancy.

The immune function of normal T cells partially depends on the maneuvering of lipid metabolism through various stages and subsets. Interestingly, T-cell malignancies also reprogram their lipid metabolism to fulfill bioenergetic demand for rapid division. The rewiring of lipid metabolism in T-cell malignancies not only provides survival benefits but also contributes to their stemness, invasion, metastasis, and angiogenesis. Owing to distinctive lipid metabolic programming in T-cell cancer, quantitative, qualitative, and spatial enrichment of specific lipid molecules occur. The formation of lipid rafts rich in cholesterol confers physical strength and sustains survival signals. The accumulation of lipids through de novo synthesis and uptake of free lipids contribute to the bioenergetic reserve required for robust demand during migration and metastasis. Lipid storage in cells leads to the formation of specialized structures known as lipid droplets. The inimitable changes in fatty acid synthesis (FAS) and fatty acid oxidation (FAO) are in dynamic balance in T-cell malignancies. FAO fuels the molecular pumps causing chemoresistance, while FAS offers structural and signaling lipids for rapid division. Lipid metabolism in T-cell cancer provides molecules having immunosuppressive abilities. Moreover, the distinctive composition of membrane lipids has implications for immune evasion by malignant cells of T-cell origin. Lipid droplets and lipid rafts are contributors to maintaining hallmarks of cancer in malignancies of T cells. In preclinical settings, molecular targeting of lipid metabolism in T-cell cancer potentiates the antitumor immunity and chemotherapeutic response. Thus, the direct and adjunct benefit of lipid metabolic targeting is expected to improve the clinical management of T-cell malignancies.

Effects of Low-Temperature Acclimation on Nutrients of Bumble Bee Bombus terrestris Queens during Prediapause and Diapause

A queen's diapause is a key period of the bumble bee life cycle that enables them to survive under unfavorable conditions. During diapause, queens fast, and nutritional reserves depend on the accumulation of nutrients during the prediapause period. Temperature is one of the most important factors affecting queens' nutrient accumulation during prediapause and nutrient consumption during diapause. Here, we used a 6-day-old mated queen of the bumble bee Bombus terrestris to evaluate the effect of temperature (10, 15, and 25 °C) and time (3, 6, and 9 days) on free water, protein, lipids, and total sugars during prediapause and at the end of 3 months of diapause. Stepwise regression analysis revealed that total sugars, free water, and lipids were much more affected by temperature than protein (p < 0.05). Lower temperature acclimation significantly increased (p < 0.05) free water and lipid accumulation by queens during prediapause. In contrast, higher temperature acclimation significantly increased (p < 0.05) protein and total sugar accumulation by queens during prediapause. The effect of temperature acclimation on the queen survival rate was not significantly different (p > 0.05) after 3 months of diapause. Moreover, lower temperature acclimation reduced protein, lipid, and total sugar consumption by queens during diapause. In conclusion, low-temperature acclimation increases queens' lipid accumulation during prediapause and reduces the nutritional consumption of queens during diapause. Low-temperature acclimation during prediapause could benefit queens by improving cold resistance and increasing reserves of major nutrient lipids during diapause.

A Perspective on the Link between Mitochondria-Associated Membranes (MAMs) and Lipid Droplets Metabolism in Neurodegenerative Diseases

Mitochondria interact with the endoplasmic reticulum (ER) through contacts called mitochondria-associated membranes (MAMs), which control several processes, such as the ER stress response, mitochondrial and ER dynamics, inflammation, apoptosis, and autophagy. MAMs represent an important platform for transport of non-vesicular phospholipids and cholesterol. Therefore, this region is highly enriched in proteins involved in lipid metabolism, including the enzymes that catalyze esterification of cholesterol into cholesteryl esters (CE) and synthesis of triacylglycerols (TAG) from fatty acids (FAs), which are then stored in lipid droplets (LDs). LDs, through contact with other organelles, prevent the toxic consequences of accumulation of unesterified (free) lipids, including lipotoxicity and oxidative stress, and serve as lipid reservoirs that can be used under multiple metabolic and physiological conditions. The LDs break down by autophagy releases of stored lipids for energy production and synthesis of membrane components and other macromolecules. Pathological lipid deposition and autophagy disruption have both been reported to occur in several neurodegenerative diseases, supporting that lipid metabolism alterations are major players in neurodegeneration. In this review, we discuss the current understanding of MAMs structure and function, focusing on their roles in lipid metabolism and the importance of autophagy in LDs metabolism, as well as the changes that occur in neurogenerative diseases.

The effect of seed priming with melatonin on improving the tolerance of Zea mays L. var saccharata to paraquat-induced oxidative stress through photosynthetic systems and enzymatic antioxidant activities

Melatonin is essential in improving plant's biotic as well as abiotic stress tolerance, especially detoxification of herbicides. The purpose of the present research study was studying the probable positive impacts of melatonin on the enhancement of the growth as well as oxidative stress tolerance in sweet corn (Zea mays L. var saccharata). For this purpose, the effects of seven treatments of priming (hydro priming with water (H), melatonin (M) priming with 25, 50, 100, 150 and 200 μM and non-priming (D)) and three doses of paraquat (PQ: 0, 5 and 7.5 L per hectare of recommended dose (5 L per hectare)) on physiological as well as biochemical responses belong to sweet corn were probed according to a completely randomized factorial design involving three replicates. The obtained results provided the indication that applying PQ, particularly at high levels (7.5 L ha−1), in the absence of priming, enhanced the rate of herbicide injury by 49.2%, as compared with 5 L ha−1, by increasing free radical production rate and membrane lipids' peroxidation, in addition to inhibiting the process of photosynthesis. Seed priming with melatonin positively impacted PQ exposed plants, even under the enhanced concentration of PQ (7.5 L ha−1). The maximum protection for the sweet corn plants could be observed at M150. Co-application of melatonin (M150) and PQ herbicide (7.5 L ha−1) reduced H2O2, MDA and herbicide injury by 3.0, 2.1 and 4.6 times, respectively, while it increased SOD, CAT, APX and POD by 2.9, 4.6, 2.4 and 2.0 times, respectively, as compared with D treatments. The obtained results provided the indication that melatonin priming could raise tolerance to oxidative stress which is triggered by PQ in sweet corn with the enhancement of antioxidant enzymes' activity and reduction of herbicide injury.

Масличность рапса : ботаническая природа , биохимические особенности и пищевой потенциал

The annual rapeseed (Brassica napus L.), a member of the Brassicaceae family, has long been used as a source of technical oil. The development of low-erucic varieties (canola, less than 5% erucic acid) by Canadian breeders in the 1970s made rapeseed a valuable and promising oilseed crop. In the Russian Federation, rapeseed oil, extracted from the seeds of the mature pods of the plant, currently ranks third after sunflower and soybean oil in terms of production volume, surpassing them in a number of biochemical parameters: optimal ratio of ω‑6: ω‑3 fatty acids (1:3–2:1), high content of oleic acid (up to 79.57%), tocopherols (45–75 mg%), carotenoids (0.30–0.57 mg%) and sterols (0.5–1.0%). The high nutritional potential of rapeseed is explained by the availabilityof winter and spring forms, domestic and foreign varieties and hybrids, successful breeding for increased oil content and resistance to diseases, good yields in many natural zones of Russia, profitability of 100–150%, sometimes up to 400% and more, despite the need for strict adherence to cultivation technology, etc. In the fatty oil industry, oil yield refers to the content of crude fat and accompanying fat-like substances that pass from the seeds into the ether extract along with the fat. The cells of oil rapeseeds contain structures, that accumulate free lipids stored by the plant for use by the growing seedling: oleosomes and possibly fat inclusions in the cytoplasm, and plastoglobules in the plastids. The formation of neutral fats is a universal mechanism for “switching off” excess primary synthesis products from plant metabolism. The qualitative fatty acid composition of seed oils of different plant species (in contrast to oils extracted from the nonseed parts of the fleshy oil fruits) is quite similar; the amount of fatty acids and the fat-soluble components extracted with them (antioxidants, vitamins, etc.) is variable. Knowledge of the botanical nature and biochemical characteristics of the oil content of rapeseed will make it possible to obtain maximum nutritional benefits through breeding, agronomic and technological methods.

A therapeutic approach of trigonella foenum graceum – a brief overview

Trigonella foenum graceum has a place in the fabaceae family fenugreek. It is composed of a variety of alkaloids, saponins, flavonoids, free fatty acids and lipids, vitamins, minerals mucilage and fibres. Trigonella foenum graceum have hepatoprotective activity, antioxidant activity, mast cell stabilizing activity, anticancer activity, antibacterial activity, cardioprotective effect and antigenotoxic activity. Traditional medicine in India and other parts of the globe has used Trigonella foenum graceum as carminative, lactation stimulant, laxative, antidiabetic effect, hypocholesterolemic influence, antioxidant potency, digestive stimulant action and hepatoprotective effect.

Potential of Single Cell Protein Production Using Waste as Growth Medium

Feed is an important factor affecting the growth of cultured fish. Around 86.5% of the production cost of aquaculture is allocated for the purchase of feed. Good quality and efficient feed is a determinant of the success of aquaculture business. Single Cell Protein (SCP) is a dry cell product of single cell microorganisms. SCP production is generally not only protein but also free amino acids, lipids, carbohydrates, vitamins and minerals. The purpose of this study was to analyze the growth media of Bacillus Cereus which will be used as a source of SCP by using some easy-to-use waste. The research method used is an experimental method using 2 isolates of Bacillus cereus bacteria, namely B. cereus strain SN7 and a consortium of bacterial isolates. Both isolates were grown in tofu liquid waste media, sago liquid waste media, and molasses waste media as a carbon source added with egg white as a nitrogen source with 3 replications in each treatment. The results showed that the most optimal growth medium for B. cereus strain SN7 and the consortium was molasses waste modified media which was incubated for 24 hours, compared to modified media for sago waste and tofu waste. For comparison, the isolates of B. cereus and consortium still had a higher growth potential than the isolates of strain SN7 which had been incubated for 24 hours.

Effects of ethanol treatment on the physicochemical properties, microstructure and protein structures of egg yolk gels

The aim of this study was to investigate the effect of ethanol treatment on the properties of egg yolk gels (EYG). The hardness of EYG showed an increasing trend, and the changes in free sulfhydryl groups manifested that ethanol treatment facilitated the formation of disulfide bonds. Environment scanning electron microscopy revealed that the degree of protein aggregation increased. Low-field nuclear magnetic resonance results indicated that ethanol treatment promoted the conversion of immobile water or lipids to free water or lipids. Moreover, the increase in the absolute of zeta potential was accompanied by the decrease in surface hydrophobicity, and the secondary structure of native egg yolk (EY) proteins underwent changes with ethanol treatment. Overall, these results demonstrated that ethanol treatment induced the structural unfolding and aggregation of EY proteins, and facilitated the better stability of the gel structures. The impact of the ethanol on the EYG increased with the concentration.

Physicochemical and biological factors determining the patchy distribution of soil water repellency among species of dominant vegetation in loess hilly region of China.

Soil water repellency (SWR) is a physical phenomenon whereby water cannot penetrate or has difficulty penetrating the soil surface. There are many factors involved in its occurrence, but the main factors controlling its emergence in loess remain unclear. In this work, we have studied numerous physicochemical and biological factors functioning in different dominant vegetations (Pinus tabulaeformis Carr., Robinia pseudoacacia L., and Hippophae rhamnoides L.) in a loess hilly region by gas chromatography–mass spectrometry (GC-MS) and high-throughput sequencing techniques. We observed that more than 75% of the soils under Robinia and Hippophae are categorized as slightly or strongly water repellent, while nearly 50% of the soils under Pinus are categorized as severely to extremely water repellent. The relative concentrations of total free lipids in the soil in the same water-repellency class were Pinus > Robinia > Hippophae, where fatty acids, alkanols, and sterols were positively correlated with SWR, whereas alkanes were not. For the abundance and diversity index of bacterial and fungal communities, the three species ranked in the following order: Robinia ≈ Hippophae > Pinus. Thus, solvent-extractable polar waxes were indicated to be better preserved in water-repellent soils under Pinus due to lower microbial diversity than Robinia and Hippophae. Here, we demonstrate polar waxes to be the principal factor controlling SWR. Moreover, the dominant phyla of fungi varied greatly than those of bacteria under three vegetation types. Correlation analysis showed that the abundance of Actinobacteria in dominant bacteria increased with SWR. Nonmetric multidimensional scaling suggested the fungal community in different water-repellent soils under Pinus to vary more than those under Robinia and Hippophae. The indicator species mainly belonged to Actinobacteria in bacteria and Basidiomycota in fungi at the phylum level; this finding was further supported by the linear discriminant analysis (LDA) effect size (LEfSe). Additionally, GC-MS identified a small amount of ergosterol, a specific biomarker of fungi under Pinus. These pieces of evidence collectively reveal that severe to extreme SWR occurs under Pinus and appears to be the most influenced by fungi and actinomycetes when the topsoil is close to air drying. However, there is a need for further testing on different plant species or land use.

QFC6, a major gene for crude fat content and quality in rice

qFC6, a major quantitative trait locus for rice crude fat content, was fine mapped to be identical with Wx. FC6 negatively regulates crude fat content and rice quality. Starch, protein and lipids are the three major components in rice endosperm. The lipids content in rice influences both storage and quality. In this study, we identified a quantitative trait locus (QTL), qFC6, for crude fat (free lipids) content through association analysis and linkage analysis. Gene-based association analysis revealed that LOC_Os06g04200, also known as Wx, was the candidate gene for qFC6. Complementation and knockout transgenic lines revealed that Wx negatively regulates crude fat content. Lipid composition and content analysis by gas chromatography and taste evaluation analysis showed that FC6 positively influenced bound lipids content and negatively affected both free lipids content and taste. Besides, higher free lipids content rice varieties exhibit more lustrous appearance after cooking and by adding extra oil during cooking could improve rice luster and taste score, indicating that higher free lipids content may make rice more lustrous and delicious. Together, we cloned a QTL coordinating rice crude fat content and eating quality and assisted in uncovering the genetic basis of rice lipid content and in the improvement of rice eating quality.

Soil warming and nitrogen addition facilitates lignin and microbial residues accrual in temperate agroecosystems

Both warming and nitrogen (N) addition affect the chemistry and characteristics of soil organic matter (SOM). However, their interactive impacts on molecular compositions and origins (plant- or microbial-derived) in agroecosystems are indeterminate. A nine-year field trial study in Northern China was undertaken to quantify the effects of warming (+2 °C), N addition (315 kg N ha−1 yr−1), and their interaction on SOM content and its composition, using biomarkers (i.e., free lipids, lignin phenols and amino sugars) and 13C NMR. Despite insignificant changes in bulk SOM content, the characteristics (i.e., molecular constituents, lability and source origin) were significantly influenced by warming and/or N addition in surface soil (0–10 cm), but not in the subsurface soil (10–20 cm). The SOM was composed of approximately 18–27% microbial residues with the bulk derived from fungi (up to 4–fold higher than bacteria). Warming alone reduced total free lipids (mainly short-chain lipids, <C20) by 21%, but increased lignin phenols (vanillyls and syringyls) by 37% and microbial residues (quantified as amino sugars) by 31%. Nitrogen addition alone increased lignin phenols by 28% and microbial residues by 30% in surface soils but had a limited effect on free lipids content. Combined warming and N addition additively increased total free lipids by 40% and lignin phenols by 42% and the ratio of fungal/bacterial microbial residues in the surface soils. Collectively, our study results suggest that warming and nitrogen addition have a synergistic positive effect on SOM persistence in temperate agroecosystem via accrual of lignin phenols and microbial residues.

Surface Properties and Architectures of Male Moth Trichoid Sensilla Investigated Using Atomic Force Microscopy

The surfaces of trichoid sensilla on male moth antennae have been sculpted over evolutionary time to capture pheromone odorant molecules emitted by the females of their species and transport the molecules in milliseconds into the binding protein milieu of the sensillum lumen. The capture of pheromone molecules likely has been optimized by the topographies and spacings of the numerous ridges and pores on these sensilla. A monolayer of free lipids in the outer epicuticle covers the sensillar surfaces and must also be involved in optimal pheromone odorant capture and transport. Using electro-conductive atomic force microscopy probes, we found that electrical surface potentials of the pores, ridges and flat planar areas between ridges varied in consistent ways, suggesting that there is a heterogeneity in the distribution of surface lipid mixtures amongst these structures that could help facilitate the capture and transport of pheromone molecules down through the pores. We also performed experiments using peak force atomic force microscopy in which we heated the sensilla to determine whether there is a temperature-related change of state of some of the surface lipid exudates such as the prominent domes covering many of the pores. We found that these exudates were unaffected by heating and did not melt or change shape significantly under high heat. Additionally, we measured and compared the topographies of the trichoid sensilla of five species of moths, including the distributions, spacings, heights and diameters of ridges, pores and pore exudates.

Clinical and Microbiological Performances and Effects on Lipid and Cytokine Production of a Ceruminolytic Ear Cleaner in Canine Erythemato-Ceruminous Otitis Externa.

Erythemato-ceruminous otitis externa (ECOE) is the most common type of otitis in dogs and is generally associated with bacterial and/or yeast infections. The performance of an ear cleaner was assessed over two weeks in canine ECOE, associated with a mild or moderate secondary infection, in a prospective open-label study. Forty ear canals with ECOE that did not receive any type of aural treatment and were not cleaned for 7 days were included. Pruritus (PS), 0–3 Otitis Index Score (OTIS-3) and 0–4 scale cytology (CYTO) scores were assessed on Day (D) 0, D7 and D14. Concentrations of a panel of 13 cytokines on the ear canal surface and the lipid profile of the exudate were measured on D0 and D14. From D0 to D12 or D13, the dogs’ ears were cleaned daily if the secretion score (SEC) was 3/3, every second day if the score was 2/3 and every third day if the score was 1/3. PS, OTIS-3, SEC and CYTO were significantly lower on D7 compared to baseline (−40%, −31%, −36%, −34%, respectively; p < 0.0001). The same parameters decreased further on D14 (−60%, −53%, −61%, −73%, respectively; p < 0.0001) and amounts of interleukin 8 and chemokine KC-like were also reduced compared to baseline (−45%, p < 0.01; −36%, p = 0.3, respectively). The lipid profile was also modified, with a decrease in free lipids and an increase in bound lipids.

Plant-derived lipids play a crucial role in forest soil carbon accumulation

Plant and microbial residues are two main sources of soil organic carbon (SOC). While recent studies have extensively examined the distribution of microbial necromass in different ecosystems, how plant residues (in particular, non-lignin components) contribute to SOC accumulation is less clear, especially in forests which make up 50% of the global soil carbon storage. Filling this knowledge gap will help us better understand SOC accumulation patterns and their response to land-use changes. Here, we analyze plant- and microbial-derived biomarkers (including lignin phenols, amino sugars, free and hydrolysable lipids) in the topsoil of major forest types in China and compare their distribution patterns together with the existing data (for lignin phenols and amino sugars) in forests and grasslands distributed globally. At the global scale, forests contain significantly less microbial necromass in SOC compared with grasslands, suggesting higher contribution of plant-derived components to forest SOC. However, plant-derived lignin phenols do not seem to play a major role in SOC accumulation, given their negative relationship with SOC contents. Instead, leaf- and root-derived hydrolysable lipids constitute a much higher proportion of SOC than lignin phenols in the investigated forests of China, even compared to grassland soils. Moreover, in contrast to lignin phenols, both SOC contents and the relative abundance of hydrolysable plant lipids in SOC increase with decreasing soil pH, increasing reactive iron and aluminum contents and with increasing lignin oxidation (indicated by acid-to-aldehyde ratios) in these forest soils. These results suggest that with increasing lignin decomposition, plant lipids and SOC accumulated via (oxyhydr)oxide protection. Collectively, our results demonstrate differential importance of plant-derived components in SOC accumulation in forests versus grasslands and highlight that plant lipids play a more important part than lignin in forest SOC accumulation. Quantitative investigations on the distribution of plant-derived lipids in addition to lignin in forest soils may help to elucidate pathways and hotspots of plant component-dominated SOC accrual.

Earlier or delayed seasonal broodstock spawning changes nutritional status and metabolic programming of growth for next-generation Atlantic salmon

Atlantic salmon (Salmo salar) breeding companies depend on changing light, temperature and feeding regimes to achieve new generations outside the natural spawning season. However, there have been few conducted trials reported that have studied whether this shift affects important traits. We test whether an induced shift of two months earlier or two months later than normal spawning season affects the nutritional status (folate, methionine, vitamin B12, vitamin B6, free amino acids, N-metabolites and lipids) in broodstock liver and muscle and whether this affects the levels of the same nutrients in the offspring. The results showed significant seasonal differences in the Cahill cycle (glucose-alanine cycle), 1C metabolism and for free amino acids catabolized in the citric acid cycle all which are important for embryonic growth The broodstock nutritional status was reflected in the eggs. Nutritional status of broodstock liver and muscle and newly fertilized eggs showed two general scenarios: Advanced spawning period did not obtain optimal deposition of nutrients in the eggs. Delayed spawning broodstock displayed a metabolic profile which indicated that it had enhanced catabolization of muscle protein which led to accumulation of aminogroups from muscle breakdown to such a degree that these amino groups were increased in the eggs. The total body weight at start-feeding stage revealed best growth for both the normal and late spawning compared to early spawning. We show here that environmental alterations in broodstock husbandry influence the nutrient status of the next generation via nutritional and metabolic programming. This is an important concept which needs more careful awareness as the metabolism compensate and regulate the energy between catabolism and anabolism through the early stages of cell divisions which give rise to changes in permanent traits for the next generation.

Potential Anti-Inflammatory Effects of a New Lyophilized Formulation of the Conditioned Medium Derived from Periodontal Ligament Stem Cells

The mesenchymal stem cells’ (MSCs) secretome includes the bioactive molecules released in the conditioned medium (CM), such as soluble proteins, free nucleic acids, lipids and extracellular vesicles. The secretome is known to mediate some of the beneficial properties related to MSCs, such as anti-inflammatory, anti-apoptotic and regenerative capacities. In this work, we aim to evaluate the anti-inflammatory potential of a new lyophilized formulation of CM derived from human periodontal ligament stem cells (hPDLSCs). With this aim, we treat hPDLSCs with lipopolysaccharide (LPS) and test the anti-inflammatory potential of lyophilized CM (LYO) through the evaluation of wound closure, transcriptomic and immunofluorescence analysis. LPS treatment increased the expression of TLR4 and of genes involved in its signaling and in p38 and NF-κB activation, also increasing the expression of cytokines and chemokines. Interestingly, LYO downregulated the expression of genes involved in Toll-like receptor 4 (TLR-4), nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and p38 signaling. As a consequence, the genes encoding for cytokines and chemokines were also downregulated. Immunofluorescence acquisitions confirmed the downregulation of TLR-4 and NF-κB with the LYO treatment. Moreover, the LYO treatment also increased hPDLSCs’ migration. LYO was demonstrated to contain transforming growth factor (TGF)-β3 and vascular endothelial growth factor (VEGF). These results suggest that LYO represents an efficacious formulation with anti-inflammatory potential and highlights lyophilization as a valid method to produce stable formulations of MSCs’ secretome.

Camellia oil Enhances Plasma Antioxidant Metabolism and Improves Plasma Lipid Metabolism in High-fat Diet-fed Rats

Living on a high-fat, high-calorie, and high-protein diet for a long period may compromise human immunity due to the long-term accumulation of free radicals and plasma lipids. The antioxidant and lipid-lowering compounds (ie polyphenols and vitamin E) in Camellia oil help to decrease the risk of numerous ailments, including cardiovascular disease (CVD), and obesity. The aims of this study were to study the hypolipidemic and antioxidant effects of Camellia oil in high-fat-fed rats and to promote the high-value use of camellia resources. The high-fat-fed rats were administrated with 2.5, 7.5, and 15 mL/kg BW Camellia oil (Camellia oil group), and 10 mg/kg BW atorvastatin (atorvastatin group), respectively, and compared with a model group (only fed with high fat) and a control group (fed with basal diet). Several parameters were measured, including (1) body weight (BW), liver-to-BW ratio; (2) plasma total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C); and (3) alanine aminotransferase (ALT), alanine aminotransferase (AST), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, model driven architecture (MDA) content, lipid metabolism-related genes, and antioxidant-related genes in liver tissue. Compared with the model group, the high-fat-fed rats in the Camellia oil and atorvastatin group had significantly lower BW and liver-to-BW ratio ( P &lt; .01), plasma TC, TG, and LDL-C levels and ALT and AST activities, but higher HDL-C levels. The relative expressions of ACAT1, DGAT2, FAS, and SREBP genes were significantly reduced in the Camellia oil and atorvastatin groups, while the relative expressions of LCAT, UCP2, MCD, and CPT-1 genes were significantly increased. The rats in the Camellia oil group showed significantly higher SOD and GSH-Px activities, significantly lower MDA content, and significantly higher relative expression of antioxidant genes (eg SOD1, GPx1, CAT, and Gclm). Thus, atorvastatin and Camellia oil exhibited significant hypolipidemic and antioxidant effects, which were better at a dose of 7.5 mL/kg (BW) of Camellia oil. Therefore, Camellia oil becomes a potential new natural resource for future research and development of antioxidant and hypolipidemic drugs, nutraceuticals, and additives.