Sexual adjustment and long-term results following vaginal reconstruction with free vascular jejunal flap are not well known.The study aims to investigate sexual adjustment and long-term results among patients who underwent vaginal reconstruction with free vascular jejunal flap.A total of 34 women, aged 16–31 years (mean 23.5), who underwent vaginal reconstruction with a vascular jejunal flap between 2005 and 2011 were evaluated. Indications for reconstruction included the following: Mayer Rokitansky Kuster Hauser syndrome (29 patients), isolated vaginal agenesis (2 patients), androgen insensitivity syndrome (2 patients), and (1) history of gynecologic-oncologic surgery.The study evaluates the perioperative results, complications, satisfaction with sexual function, length and width of the neovagina.All of the surgeries were completed without any intraoperative complications. Three patients required reoperation because of postoperative venous compromise in the grafts. The flap success rate was 100%, and no infection was observed for any case. The mean follow-up was 50 months (between 20 and 87 months). The mean vaginal depth and diameter were satisfactory for all cases. Postoperatively, six patients complained of jejunal hypersecretion, one patient complained of mucosal prolapse, and one patient complained of vaginal constriction. The neovaginal prolapse was repaired via minor surgery. Twenty-seven were married and sexually active. Twenty patients completed the questionnaire on sexual function. Sexual function was assessed using the Female Sexual Function Index (FSFI). Eleven patients were satisfied with their sexual lives after surgery (FSFI scores ≥ 25).Based on our results, satisfactory sexual function was achieved using the free jejunal vascular flap. Erman Akar M, Özkan Ö, Özkan Ö, Colak T, and Gecici O. Sexual function and long-term results following vaginal reconstruction with free vascular jejunal flap. J Sex Med 2013;10:2849–2854.