Free Fat Acid Research Articles

Abnormal lipid metabolism down-regulates adenosine triphosphate synthase β-subunit protein expression in corpus cavernosum smooth musclein vitroandin vivo

Metabolic syndrome is closely related to erectile dysfunction (ED), and hyperlipidaemia is considered a major risk factor for ED. Adenosine triphosphate (ATP) synthase is believed to play an important role in metabolic syndrome; it has been hypothesised that ATP synthase contributes to ED development. We have verified this hypothesis using primary cultured human corpus cavernosum smooth muscle (HCCSM) cells treated with excessive free fat acid (FFA) and a high-fat diet (HFD) mouse model. Our results showed that high fatty factors could cause lipid accumulation in HCCSM cells, which could result in abnormal lipid metabolism, such as high levels of triglycerides, cholesterol and glucose in the HFD mice. There was a remarkable down-regulation of ATP synthase and p-Akt after in vivo and in vitro excessive FFA treatments. These results indicated that abnormal lipid metabolism could induce ATP synthase down-regulation via the Akt phosphorylation pathway and that ATP synthase may be a target of lipotoxicity in corpus cavernosum smooth muscle cells.

ASSA13-16-4 The Influence of Combination of Amlodipine and Valsartan on Plasma Free Fat Acid and Adiponectin in Patients with Essential Hypertension

<h3>Objective</h3> To investigate the influence of combination therapy of amlodipine and valsartanon on the plasma adiponectin (ADPN) and free fatty acids (FFAs) levels in patients with essential hypertension. <h3>Methods</h3> Fifty hypertensive patients were included. After 4 week amlodipine (5mg/d) treatment, 34 patients with diastolic blood pressure (DBP) equal to or more than 90 mmHg were included in the double blind period and randomly divided into amlodipine (amlodipine, 5mg/d) and the combination (amlodipine 5mg + valsartan 80 mg/d) groups for other 8 weeks. BP, blood lipids, blood glucose, ADPN and FFA were measured at baseline, 4 and 12 week after treatment. Eighteen normotensive adults were selected as the control. <h3>Results</h3> At baseline, FFA, total cholesterol (TC) and triglycerides (TG) were significantly higher, but ADPN lower in the hypertensive group than in the control group. Four weeks amlodipine treatment increased significantly ADPN levels (4.75 ± 1.88 vs 5.09 ± 1.97 mg/L, P. <h3>Conclusions</h3> Four weeks amlodipine monotherapy can significantly increase the plasma ADPN level. On this base, the combination therapy of amlodipine and valsartan can better improve plasma ADPN and FFA than amlodipine monotherapy.

Dietary n‐3 long‐chain polyunsaturated fatty acids modify phosphoenolpyruvate carboxykinase activity and lipid synthesis from glucose in adipose tissue of rats fed a high‐sucrose diet

Long-chain polyunsaturated n-3 fatty acids (n-3 LCPUFAs) have hypolipidemic effects and modulate intermediary metabolism to prevent or reverse insulin resistance in a way that is not completely elucidated. Here, effects of these fatty acids on the lipid profile, phosphoenolpyruvate carboxykinase (PEPCK) activity, lipid synthesis from glucose in epididymal adipose tissue (Ep-AT) and liver were investigated. Male rats were fed a high-sucrose diet (SU diet), containing either sunflower oil or a mixture of sunflower and fish oil (SU-FO diet), and the control group was fed a standard diet. After 13 weeks, liver, adipose tissue and blood were harvested and analysed. The dietary n-3 LCPUFAs prevented sucrose-induced increase in adiposity and serum free fat acids, serum and hepatic triacylglycerol and insulin levels. Furthermore, these n-3 LCPUFAs decreased lipid synthesis from glucose and increased PEPCK activity in the Ep-AT of rats fed the SU-FO diet compared to those fed the SU diet, besides reducing lipid synthesis from glucose in hepatic tissue. Thus, the inclusion of n-3 LCPUFAs in the diet may be beneficial for the prevention or attenuation of dyslipidemia and insulin resistance, and for reducing the risk of related chronic diseases.

The influence of exogenous lipases on quality of dry-cured duck leg

To explore the function of the exogenous lipase on dry-cured duck leg during the whole processing, test group was treated by the exogenous lipase to compare the quality with control group.The dry-cured duck legs were taken after marinating, roasting, smoking, and on drying of the 7th, 14th, 21st, 28th day respectively, to make sensory evaluation and assay physical and chemical indexes which included moisture, water activity, free fat acid (FFA) , pH, peroxide value, TBA value and so on.Sensory evaluation showed that the optimal results appeared on 14th day of the test group and on 21st day of the control group.Physical and chemical indexes implicated that after 14 days' drying, moisture and water activity decreased to 45.9% and 0.806, respectively.Saturated fatty acids (SFA) and polyunsaturated fatty acids (PFA) in the FFA increased to 3.63g /100g fat and 2.033g /100g fat respectively.Peroxide value and TBA value increased to 0.0326g /100g fat and 0.7892mg /100g fat.The parameters of the test group on 14th day were at the same level with the ones of the control group on 21st day.Results demonstrated that comparing to control group, test group could shorten mature period about a week, to 14 days' period.

Purification, Characterization and Application of a Cold- Adapted Phospholipase A1 from Bacillus Cereus Sp. AF-1

ABSTRACTThis paper reports the purification, characterization and application of an extracellular phospholipase A1 that functions at low temperature, from Bacillus cereus sp. AF-1 isolated from soil near the oil mill in Tianjin, China. The enzyme was purified 56-fold from fermentation supernatant, with 30.19 % recovery (specific activity 1195.24 U/mg). The purified enzyme has a molecular weight of 20 kDa. Using phospholipid as substrate, the enzyme showed maximum specific activity (Vmax) of 1.302 mmol·L−1mg−1 proteins with its corresponding Km value of 11.11 mg·mL−1. The enzyme was identified as phospholipase A1 by analyzing the free fat acid of enzymic hydrolysates which was extracted with a two-phase heptane-isopropyl alcohol—water system. The optimal temperature and pH for phospholipase A1 were 35°C and 7.5, respectively. The enzyme biocatalyst retained 85% of the initial activity after 10 weeks of storage at 4°C. The phosphorus content of the crude oil could be reduced to 7 mg·kg−1 when the optimal set of variables was: enzyme dosage of 0.4 mg kg−1 and reaction time of 3 h.

The impact of sleep apnea treatment on carbohydrate metabolism in patients with acromegaly

Sleep Apnea is highly prevalent and may contribute to insulin resistance in patients with acromegaly. The primary aim of this study was to assess the impact of sleep apnea treatment with a continuous positive air pressure (CPAP) device on insulin resistance evaluated by hyperinsulinemic euglycemic clamp (HEC). A prospective, randomized, open label, placebo-controlled, crossover study was performed at a tertiary outpatient pituitary center. Twelve acromegalic subjects on somatostatin analogs (SA) with a recent diagnosis of moderate to severe sleep apnea were randomized to CPAP therapy or to nasal dilator adhesive (NDA) with placebo effect for 3 months and then crossed over for another 3 months period without washout. Assessment of HEC, mathematical insulin resistance indexes (HOMA, HOMA2 and QUICKI), GH, IGF-1, HbA1c and free fat acids were performed. A significant reduction on insulin resistance was demonstrated by HEC at the end of the study in patients on CPAP (HEC, pre- and post-CPAP: 4.27 vs. 6.10 mg/Kg/min, P = 0.032). This reduction was not observed in NDA group (HEC, pre- and post-adhesive: 5.53 vs. 5.19 mg/Kg/min, P = 0.455). There was no significant difference on HbA1c or on peripheral insulin resistance indexes in both treatments. CPAP promoted a significant increase on peripheral insulin sensitivity in acromegalic patients with moderate to severe sleep apnea on SA use. Our results support the concept that sleep apnea plays an important role on glucose metabolism. Insulin resistance indexes were unable to detect this finding.

PENGARUH BAHAN PENSUSPENSI TERHADAP AKTIVITAS PENGAWET METIL PARABEN

AnalyzeQuality Of Cooking Oil With Double Fractination In Tidiness With The Recuring Usage. Have been research concerning quality of cooking oil double fractination in tidiness with the recuring usage by aims to determine quality of cooking oil with double fractination in tidiness with the recuring usage. The parameter of quality test of oil and fat is free fat acid number, saponification number, peroxide number, iodium number and water content with using titrimetri method. Kind of cooking oil which used were cooking oil double fractination. Research result show that cooking oil which used repeatedly with 3 times intensity has reduce of quality and no safe to consumed based on what standardized by National Standarization Agency. Key W ords: methyl paraben, suspending agent,preservatif efectivity

E0191 Free fatty acids inhibit the expression of anticoagulant thrombomodulin protein C system an implication for the development of the prothrombotic state in metabolic syndrome

BackgroundMetabolic syndrome displays a significant prothrombotic state which renders patients highly susceptible to myocardial infarction, ischaemic stroke and peripheral vascular diseases. Although many clinical studies showed that the prothrombotic state...

Effects Of Chocolate Diet Associated To Exercise On Metabolic Profile And Substrate Tissue Of Rats

PURPOSE: The present study aimed to verify the effects of a high lipid diet associated to aerobic exercise on metabolic profile and substrate tissue contents of rats. METHODS: Nineteen Wistar rats (30 days old at the beginning of the experiment) were fed a chocolate diet (25.0% roasted peanut, 25.0% chocolate, 12.5% corn biscuit, 18.0% protein [casein; powdered milk protein], 4.5% cellulose, 5.0% vitamins and minerals, 10.0% fiber, totaling 22.0% of lipids) for 2 months. Further, the animals were separated into two groups: Exercise Chocolate (EC) (n=9) and Sedentary Chocolate (SC) (n=10). Exercise training protocol consisted of swimming, 5days/week (1hour/day), supporting overload equivalent to 2% of body weight, during 8 weeks, starting at 90 days old. At the end of the experiment, the animals were killed in order to determine the serum glucose, free fat acids (FFA), triglycerides, total cholesterol, HDL and LDL cholesterol. Samples of liver and muscle tissues were collected for determination of triglycerides and glycogen concentrations. The results were analyzed by student t test (P<0.05). RESULTS: Serum glucose (EC: 90.4±13.2 vs. SC: 81.7±20.5mg.dL-1), total cholesterol (EC: 69.0±5.5 vs. SC: 71.5±13.2 mg.dL-1), HDL (EC: 21±1,87 vs. SC: 20.9±3.7mg.dL1), and LDL cholesterol (EC: 35.0±5.5 vs. SC: 32.2±11.3mg.dL-1) and FFA concentrations (EC: 0.55±0.14 vs. SC: 0.68±0.19mg.dL-1) did not present differences between groups, while serum triglycerides (EC: 64.8±12.1 vs. SC: 92.1±23.4mg.dL-1) were significantly reduced by physical training. There were no significant differences in liver (EC: 0.23±0.07 vs. SC: 0.218±0.06 mg.100mg-1) and muscle glycogen contents (EC: 0.07±0.06 vs. SC: 0.041±0.03 mg.100mg-1) between groups. Exercise increased muscle triglycerides (EC: 26.7±11.0 vs. SC: 16.0±8.6 mg.100mg-1), decreased liver triglycerides (EC: 0.86±0.43 vs. SC: 1.8±0.7 mg.100mg-1) contents and was able to reduce the effects of the high lipid diet on body weight gain (EC: 22.3±18.3 vs. SC: 54.9±19.7g). CONCLUSIONS: The data suggest that the exercise performed at moderate intensity was efficient to improve serum and liver triglycerides as well as to prevent excess of body weight gain in rats fed a high lipid diet.

Synthesis of a novel multi –SO3H functionalized ionic liquid and its catalytic activities for biodiesel synthesis

A novel multi –SO3H functionalized strong Bronsted acidic ionic liquid has been prepared for the synthesis of biodiesel from rapeseed oil and methanol. The results showed that the novel catalyst was very efficient for the reaction, with excellent water and acid resistant ability. The catalyst could smoothly transform both the rapeseed oil and free fat acid to the biodiesel. Also the novel ionic liquid was insoluble in the organic phase, which made the recycling of the catalyst very convenient. Operational simplicity, low cost of catalyst used, high yields, wide applicability and reusability are the key features of this methodology. The novel catalyst therefore has great potential for use in green processes.

SUPLEMENTAÇÃO DE GLICEROL OU DE LECITINA EM DIFERENTES NÍVEIS DE ÁCIDOS GRAXOS LIVRES EM DIETAS PARA FRANGOS DE CORTE

A metabolism trial was carried out with 95 male broilers, 24 day-old, of commercial line, subjected to 18+1 treatments with 5 replications. Three levels of free fat acid (FFA) inclusion by the usage of soybean oil (SO), acidulated soybean soapstock (ASS), and the mixture between the two oils (50:50%), in two levels of inclusion (4 and 8%) in the basal diet (BD) and the supplementation of glycerol or emulsifier (0.5%), in substitution to starch were tested. The trial was conducted for 8 days. Birds performance, metabolizability coefficient of dry matter (MCDM), fat (MCCF) and energy (MCCE) were evaluated and the comparison among oils and supplements. The performance and the metabolism were not affected (P>0.05) by FFA levels or supplements tested. It was observed that the use of lecithin improved added crude fat metabolism (P KEY WORDS: Acidulated soybean soapstock, emulsification, lecithin, metabolism, performance.

Efficacy of Cr (III) Supplementation on Growth, Carcass Composition, Blood Metabolites, and Endocrine Parameters in Finishing Pigs

The study was conducted to evaluate the effects of trivalent chromium from different sources on growth, carcass composition, and serum parameters in finishing pigs. Ninety-six crossbred pigs with an initial average body weight of 65.57±1.05 kg were blocked by body weight and randomly assigned to four treatments with three replicates. Pigs were offered one of four diets including a control diet or the control diet supplemented with 200 μg/kg chromium from either chromium chloride (CrCl 3 ), chromium picolinate (CrPic) or chromium nanocomposite (CrNano) for 40 days. After completion of the feeding trial, eight pigs from each treatment were selected to collect blood samples, and slaughtered to measure carcass composition. The results showed that supplemental chromium had no significant effect on growth performance, while CrNano increased carcass lean proportion and loin Longissimus muscle area (p<0.05), and decreased carcass fat proportion and 10 th rib backfat depth (p<0.05). CrPic supplementation also resulted in lower fat proportion and larger Longissimus muscle area (p<0.05). The addition of Cr from CrNano or CrPic decreased serum glucose (p<0.05) and increased concentrations of total protein and free fat acid in serum (p<0.05). Serum urea nitrogen, triglyceride and cholesterol were decreased (p<0.05), and serum high density lipoprotein and lipase activity were increased (p<0.05) with the supplementation of CrNano. Serum insulin was decreased (p<0.05) by supplemental Cr from CrNano or CrPic, and serum insulin-like growth factor I was increased significantly in the CrNano treated group. These results suggest that chromium nanocomposite has higher efficacy on carcass composition in pigs compared to the traditional chromium sources.

Skeletal muscle lipotoxicity in insulin resistance and type 2 diabetes

Patients with insulin resistance and type 2 diabetes (T2DM) commonly exhibit elevated plasma free fat acid (FFA) and muscle fat (triglyceride) levels, which may either represent inborn errors of regulation of lipid metabolism, obesity, insulin resistance of adipose tissue or defective insulin secretion with an elevated rate of lipolysis. Alternatively, accumulation of different fat species in the circulation and tissues in T2DM may occur due to an impairment of oxidation of FFAs by the mitochondria (Petersen et al. 2005). Since the ground-breaking studies by Sir Philip Randle documenting the substrate competition between oxidation of fat and glucose, with phosphofructokinase and pyruvate dehydrogenase enzyme activities playing a key role (Randle et al. 1963), the ‘glucose fatty acid cycle’ and elevated FFA levels have been thought to play an important role in insulin resistance. Indeed, inhibition of lipolysis and FFA levels using a nicotinic acid derivative markedly improved in vivo peripheral insulin action including both oxidative and non-oxidative glucose metabolism in T2DM (Vaag et al. 1991). The extent to which increased availability of plasma FFA levels in itself impairs proximal insulin signalling transduction in healthy subjects is controversial (Storgaard et al. 2004). To this end, studies have suggested various additional, parallel or complementary mechanisms by which fatty acids adversely affect muscle insulin action and glucose uptake including degree of saturation and chain length of fatty acids as well as the conversion of fatty acids to the potentially deleterious lipid species ceramide and diaglycerol. Recent data have emerged that mitochondrial overload and limited capacity of the enzyme carnitine palmitoyl-transferase-1 (CPT-1) to transport cytosolic long-chain acyl CoA into the mitochondria may be involved in the development of fat-induced insulin resistance due to the accumulation of incompletely oxidized lipid intermediates (Koves et al. 2008).

Estudo da vida útil de queijo Minas

Foi avaliado o prazo de vida útil do queijo Minas frescal armazenado a 4 °C por 30 dias, através de análises microbiológicas e físico-químicas. Três lotes (A, B e C) de uma mesma marca foram analisados nos dias 1, 10, 20 e 30 após a fabricação. Foram feitas as contagens de microrganismos psicrotróficos, lipolíticos, proteolíticos, bactérias lácticas e mesófilas, que apresentaram um crescimento contínuo durante o período, atingindo contagens médias, respectivamente:, piscotróficos = 11,63; lipolíticos = 10,34; proteolíticos = 9,75 bactérias lácticas = 9,53; e mesófilos = 11,47 log UFC.g-1. A população de Escherichia coli desenvolveu-se em um dos lotes e Staphylococcus coagulase + e Salmonella não foram detectados. No mesmo período, observou-se a redução de 0,81 no valor do pH e o aumento do ácido láctico (0,026%). Não se observou redução na gordura (21,5%), embora a quantidade de ácidos graxos livres tenha aumentado em 0,22 mg KOH.g-1 de gordura. A proteína variou de 21,30 a 22,10% sem diferença significativa. O índice de extensão da proteólise aumentou 3% a cada 10 dias. Apenas em um dos lotes (C) no 30º dia o produto apresentou-se impróprio para o consumo com 1,7 x 10(4) NMP.g-1 de E. coli.

The progression of type 2 diabetes: Partly caused by deficiency of ASP-C5L2 pathway?

Type 2 diabetes is characterized by insulin resistance and β-cell dysfunction. The pathway of acylation-stimulating protein (ASP) and its specific receptor, C5a-like receptor 2 (C5L2), involves in the effective clearance of plasma glucose and free fat acid. Abnormal ASP-C5L2 pathway may induce insulin resistance, as well as cause hyperglycemia and elevated plasma free fat acid. High levels of plasma glucose and free fat acid induce β-cell apoptosis and dysfunction. We proposed that the abnormality of ASP-C5L2 pathway contributes to progression of type 2 diabetes.

Fluorescence Imaging with UV-Excitation for Evaluating Freshness of Rice

To measure the freshness of rice quickly and conveniently, we used a fluorescence imaging method with UV-excitation. The system consists of two UV-A fluorescent lamps and a blue LED with a band-pass filter, a cooled CCD camera with an IR cutoff filter to capture the fluorescence image on a PC, and image processing software for measuring the fluorescence intensity of the image. In a darkened box, sample materials are placed on a stage painted with non-fluorescent, flat black paint to minimize background scattering. Testing stored samples of unhulled rice and brown rice, we found a high correlation between the fluorescence intensity and conventional indices such as free fat acidity and guaiacol reaction. The fluorescence intensity increased as the storage temperature rose or the storage period lengthened. Eating quality decreased significantly as the fluorescence intensity rose. These results show that this system can estimate the loss of eating quality that occurs during storage of rice. Thus, fluorescence intensity could provide an effective and convenient way to evaluate rice quality.

Thiazolidinediones Anti-Inflammatory and Anti-Atherosclerotic Effects in Type 2 Diabetes Mellitus

Atherosclerosis is a major vascular complication of diabetes and the primary cause of mortality in patients with this disease. Inflammation has been implicated in the pathogenesis, progression and complications of both atherosclerosis and diabetes type 2, and these two complex disorders are often found intertwined in the patients. Peroxisome proliferatoractivated receptors-γ (PPARs-γ) are nuclear receptors that have been involved as transcriptional mediators in glucose homeostasis, lipid metabolism, and adipogenesis. PPAR-γ agonists, the thiazolidinediones (TZDs) are antidiabetic drugs that increase insulin sensitivity, lower blood glucose, decrease triglycerides and free fat acids and seem to have antiinflammatory effects as they reduce inflammatory markers and improve cardiovascular risk factors. All the major cell types in the vasculature express PPAR-γ including macrophages and vascular smooth muscle found cells in human atheroma. Activation of PPAR-γ by thiazolidinediones blocks vascular smooth muscle cells growth and migration thus reducing atherosclerosis. Several clinical studies have illustrated the beneficial role of thiazolidinediones in the atherosclerosis process, as they ameliorate endothelial dysfunction, reduce intima media thickness (IMT) in the carotid arteries and the restenosis rate after coronary stent implantation. However, recent trials have raised significant concerns about the deleterious action of thiazolidinediones, particularly rosiglitazone, on the cardiovascular system. Weighing the potential beneficial and harmful role of thiazolidinediones and exploring the possible mechanisms may provide a thorough view about the optimum clinical use of these compounds. Keywords: Endothelial function, PPAR-γ, macrophages, vascular smooth muscle cells, insulin resistance, dyslipidemia

Assessment of cottonseed deterioration during accelerated ageing

An experiment was carried out using an upland cotton (Gossypium hirsutum L.) cv. NIAB-78 to investigate the physiological and biochemical aspects of seed deterioration during accelerated ageing. The seeds were subjected to 40-44°C and 90-95% relative humidity for 1, 2, 3, 5, 7, 10, 15 and 20 days for artificial ageing. Controlled ageing linearly decreased the germination capacity. There was a complete loss of germination at 20 days of accelerated ageing. The decline in seed germination during accelerated ageing was accompanied with the increase in mean emergence time (MET), days to 50% germination (T 50 ), electrical conductivity (EC) of solute leakage, free fat acidity and lipid peroxidation. Accelerated ageing not only affected emergence percentage and time but also decreased the seedling vigour, as is indicated by reduction in root weight, shoot weight and hypocotyl length. The cottonseed deterioration by accelerated ageing might be due to membrane disintegration and inactivation of enzymatic systems due to lipid peroxidation and increase in free fat acidity.

Aspectos neuroendócrinos da síndrome metabólica

A síndrome metabólica (SM) é caracterizada por alterações no metabolismo glicídico, obesidade, hipertensão e dislipidemia. Estas alterações metabólicas interrelacionam-se com diversos eixos endócrinos controlados pelo hipotálamo e pela hipófise. A obesidade central parece relacionar-se a uma hiperativação do eixo hipotálamo-hipófise-adrenal, como também do sistema nervoso simpático, que poderia levar a um quadro de hipercortisolismo sub-clínico e hipertensão arterial. A SM é também um estado de hipo-somatotropismo relativo relacionado à gordura visceral. Além disso, níveis elevados de ácidos graxos livres e a hiperinsulinemia, secundários à resistência insulínica, estão relacionadas a um bloqueio do eixo somatotrófico. Em homens, a SM relaciona-se a um hipogonadismo tanto por diminuição de gonadotrofinas como por inibição direta da produção de testosterona. Já nas mulheres, existe um excesso de produção de androgênios, principalmente relacionado à hiperinsulinemia, aumento da atividade da aromatase e da liberação de LH. Desta forma, a SM é um estado relacionado a importantes modificações nos mecanismos de feedback responsáveis pelo correto funcionamento dos eixos neuroendócrinos.

Effects of an acute raw resistant potato starch supplement on postprandial glycemia, insulinemia, lipemia in healthy adults

The purpose of this study was to evaluate the lipid and glucose response after an acute intake of raw resistant potato starch. Ten healthy volunteers (6 males and 4 females, aged 19–27 years old, a body mass index of 22.4±0.8 kg·m −2) participate in the study after giving their written consent. They ingested on separate days a low fiber-low resistant starch meal (27 g proteins, 54 g carbohydrates, 24 g lipids and 390 mg cholesterol) supplemented (RSsup) or not (NoRS) with 30 g raw potato starch as a source of resistant starch. Fasting and postprandial blood data were analyzed for free fat acids, glucose, insulin, triglyceride, cholesterol, chylomicron triglyceride and cholesterol, and retinyl palmitate, during seven hours. The chylomicron was isolated and plasma retinyl palmitate (RP) was used as a tracer of chylomicron remnants clearance. The significant results, p<0.05, were: area under the curve of plasma glucose (AUC, mean normalized area against zero time) for NoRS=−32 and for RSsup=102 mmol·7h·L −1; AUC plasma retinyl palmitate NoRS=407 and RSsup=250 mg·7h·L −1; and AUC chylomicron-triglyceride NoRS=52 and RSsup=40 mmol·7h·L −1. The AUC for insulin, free fat acid, plasma triglyceride, cholesterol and chylomicron-cholesterol did not show statistical difference after both diets. The response over time for FFA was characterized by a delay insulin-driven fall with the RSsup that is an unexpected result. Sporadic intakes of potato raw resistant starch did not improve post prandial blood glucose. This unexpected conclusion must be considered with caution as the resistant starch used in this study is probably more digestible as suggested by in vitro study. However, the decrease of postprandial plasma triglycerides rich lipoprotein concentration due to a resistant starch supplement was related to an increase in the clearance of chylomicron. This finding is of interest in the coronary artery disease prevention. Sponsorship: EEC Flair program EURESTA, French Ministery of Agriculture, Danone and FAPESP-BR.