CO2 -induced chloroplast movement was reported in the monograph by Gustav Senn in 1908: unilateral CO2 supply to the one cell-layered moss leaves induced the positively CO2 -tactic periclinal arrangement of chloroplasts. Here, using the model moss plant Physcomitrium patens, we examined basic features of chloroplast CO2 -tactic relocation with a modernized experimental system. The CO2 relocation was light-dependent and, especially, CO2 relocation in red light was substantially dependent on photosynthetic activity. In blue light, CO2 relocation was mainly dependent on microfilaments while microtubule-based movement was insensitive to CO2 , whereas in red light, both cytoskeletons contributed redundantly to CO2 relocation. The CO2 relocation was observed not only when the two leaf surfaces were exposed to CO2 -free air versus CO2 -containing air, but also by exposing them physiologically relevant differences in CO2 concentrations. In the leaves placed on the surface of a gel sheet, chloroplasts avoided the gel side and positioned in the air-facing surface, and this phenomenon was also shown to be photosynthesis dependent. Based on these observations, we propose a hypothesis that the threshold light intensity between the light-accumulation and -avoidance responses of the photorelocation would be increased by CO2 , resulting in the CO2 -tactic relocation of chloroplasts.