The use of Pavlov's conditioned reflex method combined with rapid fixation of intravital changes occurring in the free ammonia level of the large cerebral hemispheres of rats enabled us to discover variations in the brain metabolism already within the first 5 seconds of the absolute differentiation with various degrees of stability, the differentiation reactions being visually absolutely uniform in character. The absence of specific rise in the ammonia level in the brain during the first seconds of a particularly stable differentiation is evidently caused by a considerable shortening of the initial phase of excitation and the intensification of the inhibitory processes concentration.
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