Structure Of Fragment Research Articles

Application of Machine Learning Methods in Predicting Lysine-specific Histone Demethylase 1 (LSD1) Inhibitors

Background: Lysine-specific histone demethylase 1 (LSD1) is a well-known anti-cancer target for drug discovery. Novel reversible inhibitors of LSD1 are desirable to be developed. Objective: This study aimed to build reliable predictive models to evaluate the antineoplastic efficacy of compounds and reveal the structural foundation underlying the inhibitory activity of LSD1. Methods: Multiple artificial intelligence algorithms were utilized in the development of quantitative structure-activity relationship (QSAR) models. A dataset comprising 915 compounds with welldefined IC50 values against LSD1 was assembled for analysis. The structures of these compounds were described by different descriptor packages. Principal component analysis (PCA) was performed to explore the chemical space distribution of each dataset. Y-randomization test and applicability domain (AD) analysis were deployed to validate the reliability of models. Results: For regression models, a consensus model was constructed by integrating the predictions of four top-performing individual models (SVM_ECFP4, RFR_PyDescriptor, RFR_RDKIT, and TRANSNNI), resulting in enhanced predictive performance as compared to the individual models. The consensus model achieved a determination coefficient (r2, for the test set) value of 0.82. For classification models, the consensus model was derived from the amalgamation of three individual models (ASNN_RDKIT, ASNN_ECFP4, and RFR_ECFP4), and the overall prediction accuracy of the model was 0.96 for external validation. Conclusion: The reliable models could be used to identify highly active LSD1 inhibitors for the purpose of efficient virtual screening. In addition, the important molecular properties and structural fragments derived from this work can provide guidance for the structural optimization of novel LSD1 inhibitors.

Coexistence of territorial competitor ants in fragmented boreal forest landscape

The distribution of species in a patchy habitat may be influenced by competitive interactions. The dominant and highly competitive boreal ant species belong to the Formica rufa group. A pair of species, Formica aquilonia and Formica polyctena, require extensive territories due to their multi-nest breeding habits. The coexistence and habitat patterns of these two wood ant species in the boreal forest landscape were investigated. Forest characteristics in the vicinity of nests in forest patches were similar for both species, but they did not coexist in the same sampling plots of 0.79 ha in forest patches, indicating competitive exclusion. The sampling plots in large forest patches were more occupied by F. aquilonia, while no such association was found for F. polyctena. At a larger spatial scale (78.5 ha), we found that F. polyctena was more tolerant of smaller forest patches than F. aquilonia suggesting that these two ant species can coexist in moderately fragmented forest landscapes. However, forest habitat loss, fragmentation and climate-induced changes in forest tree structure may shift the species balance in favour of F. polyctena over F. aquilonia in the future.

HUMIC ACIDS: PROPERTIES, STRUCTURE, AND APPLICATION

Humic substances (HSs) are a diverse class of natural compounds with no fixed chemical composition, formed from plant and microbial residues through the action of environmental factors and living organisms over many years. Despite extensive research spanning two centuries, the complex and variable nature of HSs' structure remains a subject of scientific inquiry. These substances, notably humic acids, fulvic acids, and humin, play crucial roles in ecological and environmental processes due to their abundant functional groups and resilience to biodegradation. This review explores the intricate structure and properties of HSs, their classification, and their occurrence in nature. It highlights the different models proposed to describe the structural fragments of humic acids, emphasizing their aromatic cores and diverse functional groups. The variability in the molecular weight distribution of HSs, attributed to their polydisperse nature, is also discussed, along with methods used for their determination, such as exclusion chromatography. Furthermore, the elemental and functional compositions of humic acids are examined, detailing their acid-base properties and capacity for heavy metal complexation. The synthesis of HSs from natural sources, such as soil, peat, coal, and artificial processes, is covered, showcasing methods like alkaline extraction and hydrothermal treatment. Recent advancements in artificial humification, including oxidative ammonolysis and Fenton reagent-based oxidation, are reviewed for their potential in producing environmentally friendly humic materials from lignin and waste biomass. The study concludes by underscoring the environmental significance and practical applications of HSs, particularly in agriculture, soil conditioning, and environmental remediation. The diverse properties and synthesis methods of HSs make them promising candidates for sustainable material production and environmental management. Humic acids are versatile compounds beneficial for human health due to their potent antioxidant properties, immune-modulating effects, and support for gastrointestinal health and detoxification. Structurally diverse, they feature groups like carboxyl, phenolic hydroxyl, quinones, ketonic carbonyls, amino, and sulfhydryl, contributing to their stability and amphiphilic nature. In pharmaceutical applications, they show promise for drug delivery, antioxidant therapies, wound healing, antimicrobial actions, and biofilm disruption, underlining their biocompatibility and safety. Key words:

Hydrochar from co-hydrothermal carbonization of sewage sludge and sunflower stover: Synergistic effects and combustion characteristics

This study carried out co-hydrothermal carbonization (Co-HTC) on sewage sludge (SS) and sunflower straw (SFS) at various ratios (1:0, 3:1, 1:1, 1:3, 0:1) to prepare hydrochar. The raw samples and produced hydrochar were characterized using ultimate analysis, proximate analysis, Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The potential synergistic impact during the reaction process were analyzed. As the SFS ratio increased, the fuel ratio of hydrothermal carbons rose from 0.29 to 0.37, while the higher heating value (HHV) improved from 7.44 (MJ/kg) to 20.86 (MJ/kg). The co-hydrothermal synergistic effect resulted in enhanced the hydrochar yield, energy yield, carbon sequestration rate and organic retention rate. Compared to raw sample, the surface of hydrochar exhibits a rougher and more fragmented structure, with an increased number of microspheres and micropores, resulted in an increased specific surface area. Thermogravimetric analysis (TGA) demonstrated that, in comparison to HTC of sludge alone, the Co-HTC of SS with SFS enhances the ignition temperature and delays the completion of combustion. Therefore, Co-HTC with SFS is an effective method for converting SS into clean solid fuel for energy applications.

Effect of H2O2/ascorbic acid degradation and gradient ethanol precipitation on the physicochemical properties and biological activities of pectin polysaccharides from Satsuma Mandarin

In this work, three degraded polysaccharides (DMPP-40, DMPP-60, DMPP-80) were successfully obtained by H2O2/ascorbic acid degradation and gradient ethanol precipitation from Satsuma mandarin peel pectin (MPP), and their physicochemical properties, antioxidant and prebiotic activities were investigated. The molecular weight of MPP, DMPP-40, DMPP-60, DMPP-80 were determined to be 336.83 ± 10.57, 18.93 ± 0.54, 26.07 ± 0.83 and 8.71 ± 0.27 kDa, respectively. The ethanol concentration significantly affected the physicochemical properties of DMPPs. DMPP-60 showed the highest yield (69.07 %) and uronic acid content (64.85 %), DMPP-80 showed the lowest molecular weight (8.71 kDa), and the composition and proportion of monosaccharides of DMPPs were significantly different. Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (1H NMR) confirmed that DMPPs exhibited similar functional groups, while X-ray diffraction (XRD) indicated that DMPP-40 possessed some crystallographic sequences. Scanning electron microscopy (SEM) images directly verified the fragmented structure and reduced surface area of DMPPs. Besides, the H2O2/ascorbic acid treatment could obviously reduce the apparent viscosity and thermal stability of MPP. Meanwhile, the results of bioactivity assay showed that DMPPs possessed better antioxidant activity and probiotics pro-proliferative effects compared with MPP. DMPP-80 could significantly inhibit lipopolysaccharides (LPS)-stimulated production of inflammatory factors (including nitric oxide (NO), interleukin (IL)-6, tumor necrosis factor (TNF)-α and interleukin (IL)-1β) in RAW264.7 cells. Results suggest that the H2O2/ascorbic acid combined with gradient ethanol precipitation has potential applications in degradation and separation of MPP to improve its biological activities.

Three-Dimensional Nanoscale Imaging of SiO2 Nanofiller in Styrene-Butadiene Rubber with High-Resolution and High-Sensitivity Ptychographic X-ray Computed Tomography.

SiO2 aggregates in styrene-butadiene rubber (SBR) were observed using ptychographic X-ray computed tomography (PXCT). The rubber composites were illuminated with X-rays focused by total reflection focusing mirrors, and the ptychographic diffraction patterns were collected using a CITIUS detector in the range of -75° to +75° angle of incidence. The projection images of the rubber composites were reconstructed with a two-dimensional resolution of 76 nm, and no significant structural changes were observed during the PXCT measurements. A three-dimensional image of the rubber composite was reconstructed with an isotropic resolution of 98 nm. Segmentation of SiO2 from the SBR, based on a histogram analysis of the phase shift, revealed a fragmented network structure of interconnected SiO2 aggregates.

New Trends in Arabic Novels in the Current Era

This study explores the new trends in contemporary Arabic novels, analyzing the thematic and stylistic shifts that characterize modern Arabic literature. The research highlights several emerging trends, including the diversification of themes, stylistic innovation, cultural hybridity, and the integration of technology into narrative forms. The study discusses how modern Arabic novels have expanded beyond traditional topics like love and politics to include more complex issues such as identity, migration, gender, and the impact of globalization. Stylistically, there has been a move towards more experimental approaches, with authors adopting fragmented structures, multi-layered narratives, and blending of genres. The incorporation of cultural influences from the West and the East has also enriched the content of Arabic novels, reflecting the multicultural experiences of modern Arab writers. Additionally, the use of digital platforms and technologies in storytelling is reshaping the narrative landscape, offering new methods for engaging readers. Through an analytical lens, this study offers a comprehensive look at how Arabic novels are evolving to reflect contemporary societal changes and global cultural interactions.

Construction frontier molecular orbital prediction model with transfer learning for organic materials

The frontier molecular orbitals of organic semiconductor materials play a crucial role in the performance of photoelectric devices, including organic photovoltaics (OPVs), organic light-emitting diodes (OLEDs), and organic photodetectors (OPDs). In this work, a model for predicting frontier molecular orbital of organic materials, including HOMO and LUMO levels, is established with the extreme gradient boosting algorithm and Klekota-Roth fingerprints. The correlation coefficients of HOMO or LUMO energy levels in the testing set are 0.75 and 0.84 in the transfer model from 11,626 DFT data in Harvard Energy database to 1198 experimental data in literature. The difference between the ML predicted value and the experimental value is smaller than the difference between ML prediction and DFT calculation, always less than 10%. Moreover, based on correlation and SHAP interpretability analysis, 13 key structural fragments influencing energy levels are selected to further verify the effective regulation of the frontier molecular orbital by the key structural fragments in practical applications. Considering the completely opposite regulatory functions of key structural fragments on HOMO and LUMO energy levels, four new Y6 derivatives, Y-PCP, Y-P6F, Y-PCF, and Y-P4FC, are designed to flexibly modify the HOMO and LUMO energy levels. The prediction trends of ML align closely with the computational trends from DFT. It is worth noting that the accuracy of LUMO energy level prediction by the prediction model makes up for the instability of DFT calculation on LUMO energy level. This work offers a cost-effective method to accelerate the acquisition of electronic properties of organic materials.

Trans(inter?)textual Migrations (Relations?) in the Traditional (Postmodernist?) Narrative of Salman Rushdie

Salman Rushdie's narrative style reflects a blend of traditional oral storytelling and postmodern literary techniques. This duality makes it challenging to categorize him strictly as either a traditional or postmodernist writer. His works, such as Midnight’s Children, exhibit fragmented storytelling akin to oral narratives, where the narrator frequently digresses, influenced by their environment and memories. These digressions create intertextual relationships, not just between texts but also between the text and the non-textual world, a technique which is called the "grasshopper narrative” in this paper. Rushdie's Midnight’s Children is heavily influenced by both Eastern oral traditions and Western literary techniques. The narrative is interspersed with references and borrowings from other texts and traditions, creating a tapestry of intertextual and trans-textual relations. For instance, characters and motifs from Forster’s A Passage to India and Kipling’s Kim find their way into Rushdie’s narrative, creating a dialogue between these works and Rushdie’s own. Moreover, the novel’s fragmented structure and the presence of a listener, Padma, enhance its oral storytelling feel, reinforcing its connection to traditional Eastern narratives. The influence of Western authors like Günter Grass and Gabriel García Márquez is also evident. These influences blend with Rushdie’s use of magic realism and his unique narrative style. Furthermore, Rushdie’s cinematic influences and his use of film vocabulary add another layer to his complex narrative structure. Rushdie’s narrative technique is a hybrid of traditional oral storytelling and postmodernist intertextuality, creating rich, multi-layered texts that traverse cultural and textual boundaries.

A stochastic and analytical model of hierarchical fragmentation

Context. Molecular clouds are the most important incubators of young stars clustered in various stellar structures whose spatial extension can vary from a few AU to several thousand AU. Although the reality of these stellar systems has been established, the physical origin of their multiplicity remains an open question. Aims. Our aim was to characterise these stellar groups at the onset of their formation by quantifying both the number of stars they contain and their mass using a hierarchical fragmentation model of the natal molecular cloud. Methods. We developed a stochastic and predictive model that reconciles the continuous multi-scale structure of a fragmenting molecular cloud with the discrete nature of the stars that are the products of this fragmentation. In this model a gas structure is defined as a multi-scale object associated with a subregion of a cloud. Such a structure undergoes quasi-static subfragmentation until star formation. This model was implemented within a gravo-turbulent fragmentation framework to analytically follow the fragmentation properties along spatial scales using an isothermal and adiabatic equations of state (EOSs). Results. We highlighted three fragmentation modes depending on the amount of fragments produced by a collapsing gas structure, namely a hierarchical mode, a monolithic mode, and a mass dispersal mode. Using an adiabatic EOS we determined a characteristic spatial scale where further fragmentation is prevented, around a few tens of AU. We show that fragmentation is a self-regulated process as fragments tend to become marginally unstable following a M ∝ R Bonnor–Ebert-like mass-size profile. Supersonic turbulent fragmentation structures the cloud down to R ≈ 0.1 pc, and gradually turns into a less productive Jeans-type fragmentation under subsonic conditions so hierarchical fragmentation is a scale dependant process. Conclusions. Our work suggests that pre-stellar objects resulting from gas fragmentation, have to progressively increase their accretion rate in order to form stars. A hierarchical fragmentation scenario is compatible with both the multiplicity of stellar systems identified in Taurus and the multi-scale structure extracted within NGC 2264 molecular cloud. This work suggests that hierarchical fragmentation is one of the main mechanisms explaining the presence of primordial structures of stellar clusters in molecular clouds.

Design, Synthesis, and Anticancer and Antibacterial Activities of Quinoline-5-Sulfonamides.

A series of new unique acetylene derivatives of 8-hydroxy- and 8-methoxyquinoline- 5-sulfonamide 3a-f and 6a-f were prepared by reactions of 8-hydroxy- and 8-methoxyquinoline- 5-sulfonyl chlorides with acetylene derivatives of amine. A series of new hybrid systems containing quinoline and 1,2,3-triazole systems 7a-h were obtained by reactions of acetylene derivatives of quinoline-5-sulfonamide 6a-d with organic azides. The structures of the obtained compounds were confirmed by 1H and 13C NMR spectroscopy and HR-MS spectrometry. The obtained quinoline derivatives 3a-f and 6a-f and 1,2,3-triazole derivatives 7a-h were tested for their anticancer and antimicrobial activity. Human amelanotic melanoma cells (C-32), human breast adenocarcinoma cells (MDA-MB-231), and human lung adenocarcinoma cells (A549) were selected as tested cancer lines, while cytotoxicity was investigated on normal human dermal fibroblasts (HFF-1). All the compounds were also tested against reference strains Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212 and representatives of multidrug-resistant clinical isolates of methicillin-resistant S. aureus (MRSA) and vancomycin-resistant E. faecalis. Only the acetylene derivatives of 8-hydroxyquinoline-5-sulfonamide 3a-f were shown to be biologically active, and 8-hydroxy-N-methyl-N-(prop-2-yn-1-yl)quinoline-5-sulfonamide (3c) showed the highest activity against all three cancer lines and MRSA isolates. Its efficacies were comparable to those of cisplatin/doxorubicin and oxacillin/ciprofloxacin. In the non-cancer HFF-1 line, the compound showed no toxicity up to an IC50 of 100 µM. In additional tests, compound 3c decreased the expression of H3, increased the transcriptional activity of cell cycle regulators (P53 and P21 proteins), and altered the expression of BCL-2 and BAX genes in all cancer lines. The unsubstituted phenolic group at position 8 of the quinoline is the key structural fragment necessary for biological activity.

Molecular Hydrogen Formation via Vibrational Excitation of Partially Superhydrogenated Pyrenes

While polycyclic aromatic hydrocarbons (PAHs) are now accepted to be abundant in interstellar space, the abundance and influence of superhydrogenated PAHs (HPAHs) in the interstellar medium (ISM) are still under investigation. HPAHs may act as catalysts for or reactants in small-molecule formation via hydrogen abstraction reactions, H2 evaporation, and carbon skeleton fragmentation. Here, we present a gas-phase infrared (IR) action spectroscopy study of the HPAH 4, 5, 9, 10-tetrahydropyrene (THP; C16H14), performed at the Free Electron Lasers for Infrared eXperiments facility. IR action spectroscopy was performed on the THP cation, protonated THP, and their fragments produced by collision-induced dissociation in the range from 600 to 1800 cm−1. Calculated IR spectra, at the density functional theory level, agree with experimental IR spectra to a high degree and were utilized to determine molecular structures of the HPAH fragments. Molecular dynamics simulations compared with experimental mass spectra reveal favorable HPAH fragmentation pathways. Molecular hydrogen (H2) is observed to be a primary fragment of [THP+H]+ with superhydrogenated duo groups. This contrasts the notion that HPAHs typically undergo carbon skeleton fragmentation leading to C x H y formation. These observations show that lowered symmetry and duo or trio aliphatic groups on HPAHs uniquely change their IR spectra, stability, and fragmentation patterns. As a result, these species may contribute to H2 formation in the ISM.

Evaluation of ultrasound-assisted tomato sour soup marination on beef: Insights into physicochemical, sensory, microstructural, and flavour characteristics

This study evaluated the quality attributes of tomato sour soup marinade and investigated the effects of ultrasound-assisted marination on the physicochemical properties, microstructure, texture, sensory quality, and flavour profile of beef. The results showed that tomato sour soup significantly increased the marinade absorption rate and improved beef tenderloin’s physicochemical properties, texture, and flavour attributes compared to static brine (P < 0.05), with organic acids playing an essential role in the marinade tenderisation process. Compared to static sour soup marination, ultrasound treatment significantly accelerated the marination process, reducing beef’s shear force, hardness, and chewiness while increasing its tenderness. Microstructural observations revealed that sour soup marination induced a fragmented and irregular muscle fibre structure. Furthermore, sour soup marination significantly increased the relative concentrations of volatile flavour compounds, including alkanes, organic sulphides, alcohols, aldehydes, and aromatic compounds. Appropriate ultrasound treatment positively affects the texture and flavour characteristics of beef marinated with tomato sour soup, and the optimal approach was 320 W ultrasound treatment for 60 min. Overall, tomato sour soup improved beef’s textural and flavour attributes, while ultrasound-assisted marination is an effective processing method to improve the quality of meat products.

Peculiarities of achieving the sustainable development goals in interaction wit private international law

Regrettably, the triple global catastrophe of pollution, natural loss, and climate change coexists with the global socioeconomic problems of poverty and inequality. Temperatures are increasing at an unprecedented rate; biodiversity is disappearing swiftly, with potentially dire consequences for all of us; and pollution is becoming a global issue, killing millions of people annually and continuing to worsen. There is no normative vacuum in which the 169 targets and 17 Sustainable Development Goals (SDGs) were born or placed. They are based on international law and designed to be compliant with the commitments made in a number of soft law instruments and international agreements. There is, of course, a connection between international law and the SDGs. What kind of relationship do they have? How much and how may the SDGs and international law complement one another to improve systems integration .In an effort to leverage the relationship for global sustainability, this paper investigates these issues from two angles. First, do the SDGs play a key role in bringing together different international organizations to work toward the ultimate goal of sustainable development? Most international institutions function in relative isolation and may pursue conflicting interests since they are engrossed in their respective mandates and goals. Goal-setting, as a governance technique to prioritize, motivate, and provide direction, has been proposed by some observers as a way to improve the overall performance of current institutions in fostering sustainable development. Insofar as providing consistency to an otherwise divergent and even inconsistent set of institutional arrangements, how effective are the SDGs likely to be? Second, is it conceivable that international law will aid in the integrated implementation of the SDGs? The SDGs themselves have come under fire for lacking consistency, even though they pride themselves on being “integrated and indivisible. This is a problem since the Millennium Development Goals (MDGs) experience has demonstrated that achieving these governance goals on their own could have unforeseen consequences. While certain MDG targets were achieved, the MDGs’ spirit was not. Then, despite its fragmented structure, how and to what degree may international law integrate the SDGs and aims and aid in the achievement of long-term sustainable development.

The Structural Basis for Recognition of Human Leukocyte Antigen Class I Molecules by the Pan-HLA Antibody W6/32.

The central immunological role of HLA class I (HLA-I) in presenting peptide Ags to cellular components of the immune system has been the focus of intense study for >60 y. A confounding factor in the study of HLA-I has been the extreme polymorphism of these molecules. The mAb W6/32 has been a fundamental reagent bypassing the issue of polymorphism by recognizing an epitope that is conserved across diverse HLA-I allotypes. However, despite the widespread use of W6/32, the epitope of this Ab has not been definitively mapped. In this study, we present the crystal structure of the Fab fragment of W6/32 in complex with peptide-HLA-B*27:05. W6/32 bound to HLA-B*27:05 beneath the Ag-binding groove, recognizing a discontinuous epitope comprised of the α1, α2, and α3 domains of HLA-I and β2-microglobulin. The epitope comprises a region of low polymorphism reflecting the pan-HLA-I nature of the binding. Notably, the W6/32 epitope neither overlaps the HLA-I binding sites of either T cell Ag receptors or killer cell Ig-like receptors. However, it does coincide with the binding sites for leukocyte Ig-like receptors and CD8 coreceptors. Consistent with this, the use of W6/32 to block the interaction of NK cells with HLA-I only weakly impaired inhibition mediated by KIR3DL1, but impacted HLA-LILR recognition.

Abstract Tu120: Sarm1 promotes diabetic cardiomyopathy by regulating HIF signaling, lipotoxicity and mitochondrial dysfunction

Heart failure occurs at twice the rate in diabetic patients as compared to normal patients, and reduced NAD levels are associated with diabetic cardiomyopathy. Sterile Alpha and Tir Motif Containing 1 (SARM1) is an intracellular NAD hydrolase. We hypothesized that SARM1 promotes NAD decline and diabetic cardiomyopathy. We subjected wild type (WT) and global SARM1 knockout mice to chronic diabetic stress induced by streptozotocin injections. We showed that 16-week diabetic stress promoted progressive decline in systolic and diastolic function, and in NAD levels, which were ameliorated by SARM1 deficiency despite similar plasma glucose and lipid levels. Gene Module Network Analysis of cardiac transcriptomics identified upregulation of genes in fatty acid and glucose metabolic processes, and suppression of genes in mitochondrial processes in diabetic WT hearts. SARM1 deficiency reversed upregulated fatty acid metabolic genes but had no impact on glucose metabolic and mitochondrial gene expressions in diabetic hearts. By transmission electron microscopy and Oil Red O staining, we observed increases in lipid droplet sizes and lipid accumulation in diabetic WT hearts, which were suppressed by SARM1 deficiency. SARM1 deficiency did not reverse mitochondrial gene expression, but it mitigated the reduction in ADP-driven respiration, mitochondrial fragmentation, and abnormal cristae structure in diabetic WT hearts. Diabetic WT hearts had increased phospho-PDH and PDK4 levels as well as activated HIF signaling, exhibiting an increase in expression of HIF-1a target genes (55 genes) , many of which were related to lipid storage. SARM1 deficiency suppressed expression of HIF1-a target genes (53 genes) , and reduced phospho-PDH and PDK4 levels. These data suggest that SARM1 regulates pseudohypoxic HIF-dependent signaling in diabetic hearts. In summary, our current data support the hypothesis that SARM1 regulates HIF-dependent transcription events, particularly those influencing lipid metabolism of diabetic hearts. However, SARM1 promotes mitochondrial dysfunction in diabetic hearts independent of transcription of mitochondrial genes. Further studies will continue to elucidate the role of SARM1 in diabetic cardiomyopathy, and its potential as a novel therapeutic target.

Structural Fragmentation and Acquisition Performance: The Role of the Target’s Corporate Strategy

Structural Fragmentation and Acquisition Performance: The Role of the Target’s Corporate Strategy

Comparative analysis of electrophoresis and interferometric optical detection method for molecular weight determination of proteins

Analytical detection methods play a pivotal role in scientific research, enabling the identification and quantification of specific analytes in various disciplines. This scientific report aims to compare two very different methodologies for determining the Molecular Mass (MM, also known as Molecular Weight, MW) of proteins: electrophoresis gel and the Interferometric Optical Detection Method (IODM). For this purpose, several proteins with different MM were selected.The electrophoresis technique was employed to validate the structure and MM of different parts or fragments of the Matrix Metallopeptidase 9 antibody (anti-MMP9), antibody against S100 calcium binding protein A6 (anti-S100A6) and Cystatin S4 antibody (anti-CST4) by examining the presence of bands with expected sizes. The IODM was applied to study the above-mentioned proteins (part of the antibodies) together with the protein G, as a reference to correlate the MM and protein sizes with the measured signal.We report the evidence of IODM as a competitive analytical approach for the determination of the MM of proteins for the first time. This innovative method allows for accurate MM determination using minimal sample volumes and concentrations, employing a simple experimental procedure that eliminates the requirement for protein denaturation.

Food Safety Governance in South Africa

This paper reviews public policy with a focus on food safety governance. In this study, food safety governance refers to the policy, institutions, and actors involved in forming, interpreting, implementing, and enforcing food safety policies. South Africa is faced with various food safety challenges, resulting from a fragmented governance structure. Hence, better governance of food safety is needed to facilitate effective enforcement. In this study, food safety governance and the challenges of an effective food control system were reviewed. The aim of this review is to assess and analyse the existing state of food safety governance in South Africa and highlight the need for better governance to address the identified challenges and ultimately enhance the effectiveness of food safety enforcement. The challenges that were identified included inconsistencies in the interpretation and implementation of standards and regulations, poor industry participation, poor enforcement of legislation, inefficiencies, lack of a national food safety policy, lack of coordination and cooperation among government at the national level, and lack of communication between the different departments and levels of government involved in the regulation of food safety. The fragmented government structure makes it difficult for the regulators to communicate with the broader industry. Consequently, in South Africa, there is a need to create a robust regulatory framework for food safety that is effectively communicated, fairly implemented, and enforced by the appropriate entities with the necessary training.

Ultrasonication: An Efficient Alternative for the Physical Modification of Starches, Flours and Grains.

Ultrasonic (USC) treatments have been applied to starches, flours and grains to modify their physicochemical properties and improve their industrial applicability. The extent of the modification caused by USC treatment depends on the treatment conditions and the natural characteristics of the treated matter. Cavitation leads to structural damage and fragmentation and partial depolymerization of starch components. The amorphous regions are more susceptible to being disrupted by ultrasonication, while the crystalline regions require extended USC exposure to be affected. The increased surface area in USC-treated samples has a higher interaction with water, resulting in modification of the swelling power, solubility, apparent viscosity, pasting properties and gel rheological and textural properties. Starch digestibility has been reported to be modified by ultrasonication to different extents depending on the power applied. The most important treatment variables leading to more pronounced modifications in USC treatments are the botanical origin of the treated matter, USC power, time, concentration and temperature. The interaction between these factors also has a significant impact on the damage caused by the treatment. The molecular rearrangement and destruction of starch structures occur simultaneously during the USC treatment and the final properties of the modified matrix will depend on the array of treatment parameters. This review summarizes the known effects of ultrasonic treatments in modifying starches, flours and grains.