Fragmented Landscapes Research Articles

Flower-bee versus pollen-bee metanetworks in fragmented landscapes.

Understanding the organization of mutualistic networks at multiple spatial scales is key to ensure biological conservation and functionality in human-modified ecosystems. Yet, how changing habitat and landscape features affect pollen-bee interaction networks is still poorly understood. Here, we analysed how bee-flower visitation and bee-pollen-transport interactions respond to habitat fragmentation at the local network and regional metanetwork scales, combining data from 29 fragments of calcareous grasslands, an endangered biodiversity hotspot in central Europe. We found that only 37% of the total unique pairwise species interactions occurred in both pollen-transport and flower visitation networks, whereas 28% and 35% were exclusive to pollen-transport and flower visitation networks, respectively. At local level, network specialization was higher in pollen-transport networks, and was negatively related to the diversity of land cover types in both network types. At metanetwork level, pollen transport data revealed that the proportion of single-fragment interactions increased with landscape diversity. Our results show that the specialization of calcareous grasslands' plant-pollinator networks decreases with landscape diversity, but network specialization is underestimated when only based on flower visitation information. Pollen transport data, more than flower visitation, and multi-scale analyses of metanetworks are fundamental for understanding plant-pollinator interactions in human-dominated landscapes.

Effects of landscape, resource use, and body size on genetic structure in bee populations.

Quantifying genetic structure and levels of genetic variation are fundamentally important to predicting the ability of populations to persist in human-altered landscapes and adapt to future environmental changes. Genetic structure reflects the dispersal of individuals over generations, which can be mediated by species-level traits or environmental factors. Dispersal distances are commonly positively associated with body size and negatively associated with the amount of degraded habitat between sites, motivating the investigation of these potential drivers of dispersal concomitantly. We quantified genetic structure and genetic variability within populations of seven bee species from the genus Euglossa across fragmented landscapes. We genotyped bees at SNP loci and tested the following predictions: (1) deforested areas restrict gene flow; (2) larger species have lower genetic structure; (3) species with greater resource specialization have higher genetic structure; and (4) sites surrounded by more intact habitat have higher genetic diversity. Contrasting with previous work on bees, we found no associations between body size and genetic structure. Genetic structure was higher for species with greater resource specialization, and the amount of intact habitat between or surrounding sites was positively associated with parameters reflecting gene flow and genetic diversity. These results challenge the dominant paradigm that individuals of larger species disperse farther, and they suggest that landscape and resource requirements are important factors mediating dispersal.

Assessing the influence of simulated environmental gradients on the spatial heterogeneity of landscape patterns in the Tibetan Plateau

Landscape patterns are pivotal in the realms of land use planning and ecological development, yet there remains a dearth of comprehensive research pertaining to the prediction of changes in landscape pattern characteristics. Within this study, we adopt the PLUS-CA-Markov and Fragstats models to forecast landscape patterns on the Tibetan Plateau spanning the period from 2030 to 2050. Through qualitative and quantitative analyses, we explore the spatiotemporal characteristics of landscape pattern changes between 2000 and 2050, concurrently identifying correlations among landscape pattern indices. Moreover, acknowledging the distinctive environmental gradients encompassing the plateau, notably elevation, slope, temperature, and precipitation, we investigate their implications on landscape pattern changes. Our findings indicate that: (1) Grassland degradation exhibited the utmost severity between 2000 and 2020, primarily attributed to overgrazing and climate-induced glacial melt. In contrast, cropland, forest, and water showcased divergent trends from 2020 to 2050 when compared to the preceding two decades, indicative of the efficacy of climate change control measures. (2) The distribution of landscape patterns on the Tibetan Plateau exhibited a considerable level of instability, marked by a decline in aggregation, reduced diversity and complexity, and amplified ecological connectivity between 2000 and 2020, signifying a partial amelioration in ecological quality. Between 2020 and 2050, landscape aggregation decreased alongside landscape fragmentation and the number of connectivity paths, signifying a discernible degradation of the plateau's ecosystem. (3) The most significant trade-off relationship was observed between landscape division index and largest patch index, while the synergistic relationship between landscape shape index and mean shape index was more pronounced. (4) Landscape aggregation, division, and largest patch index demonstrated non-linear quadratic trends in relation to elevation and temperature. Landscape shape index and patch density exhibited irregular non-linear effects. Largest patch index was predominantly influenced by slope, whereas division index was most affected by precipitation.

Spatial patterns of urban expansion and cropland loss during 2017–2022 in Guangdong, China

Urban expansion often occurs at the expense of cropland loss, posing challenges to sustainable urban growth and food security. However, detailed investigations into urban expansion and cropland loss remain limited, particularly in regions with varying levels of urbanization. Here, we take Guangdong Province, China, as a case study to exemplify how urban expansion affects cropland using remotely sensed land use products. We adopted geospatial analysis, correlation indicators, and landscape metrics to uncover their spatial relationships at 10-m spatial resolutions. Results showed that urban areas increased by 6335 km2 while cropland decreased by 3780 km2 from 2017 to 2022. Notably, 41 % of newly expanded urban areas were from croplands, and 45 % of lost croplands were converted to urban areas. Western Guangdong experienced the largest extent of urban expansion and cropland loss, emerging as a hotspot region in recent years. Additionally, our analysis observed the increasing compactness of urban areas and the growing fragmentation of cropland landscapes over time. These findings shed light on the intricate dynamics between urban expansion and cropland loss in rapidly urbanizing regions, which provide valuable insights for sustainable urban development, agricultural practice, and land management in the future.

Monitoring the Landscape Pattern Dynamics and Driving Forces in Dongting Lake Wetland in China Based on Landsat Images

Dongting Lake wetland is a typical lake wetland in the Middle and Lower Yangtze River Plain in China. Due to the influence of natural and human activities, the landscape pattern has changed significantly. This study used 12 Landsat images from 1991 to 2022 and applied three common classification methods (support vector machine, maximum likelihood, and CART decision tree) to extract and classify the landscape information, with the latter having a superior annual accuracy of over 90%. Based on the CART decision tree classification results, the dynamic characteristics of wetland spatial patterns were analyzed through the landscape pattern index, dynamic degree model, and transition matrix model. Redundancy and grey correlation analysis were employed to investigate the driving factors. The results showed increased landscape fragmentation, reduced heterogeneity, and increased complexity from 1991 to 2022. The water and mudflat areas exhibited three distinct stages: gradual decline until 2001 (−3.06 km2/a); sharp decrease until 2014 (−19.44 km2/a); and steady increase (22.93 km2/a). Vegetation conversion, particularly between sedge and reed, dominated the change in landscape pattern. Reed area initially increased (18.88 km2/a), then decreased (−35.89 km2/a), while sedge showed the opposite trend. Woodland area fluctuated, peaking in 2016 and declined by 2022. The construction of the Three Gorges Dam significantly altered landscape dynamics through water level changes, reflected by a 4.03% comprehensive dynamic degree during 2001–2004. Potential evaporation also emerged as a significant natural factor, exhibiting a negative correlation with the landscape index. During 1991–2001 and 2004–2022, the comprehensive explanatory rates of temperature, precipitation, potential evaporation, and water level on landscape pattern dynamics were 88.56% and 52.44%, respectively. Other factors like policies and socio-economic factors played a crucial role in wetland change. These findings offer valuable insights into the dynamic evolution and driving mechanisms of Dongting Lake wetland.

Dissimilar forests along the Rio Doce watershed call for multiple restoration references to avoid biotic homogenization

An environmental disaster caused by the rupture of a mining tailings dam has impacted a large area of the Rio Doce watershed in the Brazilian Atlantic Forest, resulting in unprecedented damage at spatial and temporal scales. The Atlantic Forest is one of the world's most important biodiversity hotspots. A long history of land use conversion has resulted in a highly fragmented landscape. Despite numerous restoration initiatives, these efforts have often biased criteria and use limited species assemblages. We conducted a comprehensive synthesis of the plant community in riparian forests along the Rio Doce watershed. Our work detailed vegetation composition (tree and sapling strata) and examined its relationship with edaphic and landscape factors, aiming to inform restoration projects with scientifically robust knowledge. A total of 4906 individuals from the tree strata and 4565 individuals from the sapling strata were recorded, representing a total of 1192 species from 75 families. Only 0.8% of the tree species and 0.5% of the sapling species occurred in all sampled sectors, with over 84% of the species occurring in a single watershed sector for both strata. We observed a high species heterogeneity modulated by turnover (92.3% in the tree, and 92.7% in the sapling strata) among sites. Overall, our research revealed a gradient of soil fertility influencing species composition across different strata. Additionally, we discovered that preserved landscapes had a positive impact on species diversity within both strata. The species exclusivity in the sampled sites and the high turnover rate imply the need to consider multiple reference ecosystems when restoring the watershed to reduce the risk of biotic homogenization. Finally, the reference ecosystems defined here serve as a basis for the selection of locally particular species in the implementation of restoration projects that aim to improve biodiversity, ecosystem services, and water security.

Unraveling the unknown: Adaptive spatial planning to enhance climate resilience for the endangered Swamp Grass-babbler (Laticilla cinerascens) with habitat connectivity and complexity approach

The endangered and poorly known Swamp Grass-babbler, Laticilla cinerascens (Passeriformes: Pellorneidae), confronts critical threats and vulnerability due to its specific habitat requirements and restricted populations in the northeastern region of the Indian Subcontinent. This study investigates the distribution of the species, habitat quality, geometry and shape complexity of connectivity among the protected areas (PAs), and responses to climate change in Northeast India under different climate change pathways by utilizing ensemble distribution models, and ecological metrics. From the total distribution extent (1,42,000 km2), approximately 9366 km2 (6.59 %) is identified as the suitable habitat for this threatened species. Historically centered around Dibru Saikhowa National Park (DSNP), the species faced a drastic decline due to anthropogenic activities and alteration in land use and lover cover. The study also reveals a significant decline in suitable habitat for L. cinerascens in future climate scenarios, with alarming reductions under SSP126 (>10 % in the timeframe 2041–2060 and > 30 % from 2061 to 2080), SSP245 (>90 % in both time periods), and SSP585 (>90 % in both timeframes) from the present scenario. At present, DSNP has the most suitable habitat within the distribution range but is projected to decline (>90 %) under more severe climate change scenarios, as observed in other PAs. Landscape fragmentation analysis indicates a shift in habitat geometry, highlighting the intricate impact of climate change. It predicts a substantial 343 % increase (in the SSP126) in small habitat patches in the future. Connectivity analysis among PAs shows a significant shift, with a decline exceeding 20 %. The analysis of shape complexity and connectivity geometry reveals a significant increase of over 220 % in the fragmentation of connectivity among PAs between 2061 and 2080 under the SSP585 climate change scenario compared to the present conditions. The study underscores the urgent need for conservation actions, emphasizing the complex interplay of climate change, habitat suitability, and fragmentation. Prioritizing PAs with suitable habitats and assessing their connectivity is crucial. Adaptive management strategies are essential to address ongoing environmental changes and safeguard biodiversity. Future research in critical areas is needed to establish long-term monitoring programs to lead/extend effective conservation strategies.

Influence of environmental conditions and the fragmented landscape on the co-occurrence patterns of the ichthyofauna of a stream in southwestern Brazilian Amazon

Influence of environmental conditions and the fragmented landscape on the co-occurrence patterns of the ichthyofauna of a stream in southwestern Brazilian Amazon

Assessing landscape fragmentation and ecological connectivity to support regional spatial planning: A case study of Jiangsu province, China

Urbanization in China has led to land-use changes resulting in landscape fragmentation, natural habitats loss and ecological connectivity decline. Aiming to support sustainable landscape planning, we proposed an integrated method for ecological structure assessment and optimization including morphological spatial pattern analysis (MSPA), minimal cumulative resistance (MCR) model and circuit theory to assess landscape fragmentation and ecological connectivity change at regional scale. A modified connectivity index is also proposed by employing the index of relative quality of ecological patches and corridors. We applied the combined methods to Jiangsu province in eastern China for multi-temporal landscape analysis and optimization. The results showed that the combined analysis revealed both fragmentation trend of ecological patches and ecological connectivity reduction with regard to all the species as a whole in the study area from 2000 to 2018. While, the trend has been slowed down to a certain extent lately, which is related to a series of ecological protection policies implemented in the study area, including ecological redlines policy, national park construction and returning marginal farmland into forest or grassland. Furthermore, suggestions for improving the ecological network in the study area were proposed. We proposed to improve the ecological network by fostering twenty-five potential ecological sources, maintaining twenty-five pinch points, restoring eleven dredging corridors and planning fifty-three stepping stones. Overall, we argue that the combined method can reveal both structural connectivity and functional connectivity, and the time series analysis can provide dynamics situation for better understanding and planning landscape process. It can also provide planners with a valuable strategic tool that advances the practical utility of landscape ecology techniques for spatial planning, and the combined application of the method helps to reduce some of the limitations of each individual method.

Warming effects on lizard gut microbiome depend on habitat connectivity.

Climate warming and landscape fragmentation are both factors well known to threaten biodiversity and to generate species responses and adaptation. However, the impact of warming and fragmentation interplay on organismal responses remains largely under-explored, especially when it comes to gut symbionts, which may play a key role in essential host functions and traits by extending its functional and genetic repertoire. Here, we experimentally examined the combined effects of climate warming and habitat connectivity on the gut bacterial communities of the common lizard (Zootoca vivipara) over three years. While the strength of effects varied over the years, we found that a 2°C warmer climate decreases lizard gut microbiome diversity in isolated habitats. However, enabling connectivity among habitats with warmer and cooler climates offset or even reversed warming effects. The warming effects and the association between host dispersal behaviour and microbiome diversity appear to be a potential driver of this interplay. This study suggests that preserving habitat connectivity will play a key role in mitigating climate change impacts, including the diversity of the gut microbiome, and calls for more studies combining multiple anthropogenic stressors when predicting the persistence of species and communities through global changes.

Impacts of land use/land cover on water quality: a contemporary review for researchers and policymakers

Abstract Few factors are as important in determining water quality as land use/land cover (LULC). Many land use activities, including agriculture, urban development, mining, and commercial forestry, tend to be sources of diffuse pollution. By contrast, indigenous vegetation can act as a sink, thus providing some protection from diffuse anthropogenic contamination. Notwithstanding the large body of research demonstrating these facts, decision-makers require clear and accessible information to assist them in developing effective management plans that are fully cognisant of the manifold impacts of LULC on water resources. Reviewing the available literature, this article, therefore, offers a critical overview of the typical impacts of LULC on water quality. An important strategy for managing water quality highlighted in this article is the maintenance of a sufficient amount of unfragmented natural vegetation, not only within riparian zones but also across catchment areas. However, knowledge gaps identified in this review indicate that further context-specific research is required to determine not only the types and minimum amount of vegetative cover required to protect water resources from diffuse pollution but also the potential impact of landscape fragmentation on the ability of natural vegetation to protect water resources. A critical discussion of these factors is therefore provided.

Assessing the responses of ecosystem patterns, structures and functions to drought under climate change in the Yellow River Basin, China

Understanding how ecosystems respond and adapt to drought has become an urgent issue as drought stress intensifies under climate change, yet this topic is not fully understood. Currently, conclusions on the response of ecosystems in different regions to drought disturbance are inconsistent. Based on long MODIS data and observed data, this study systematically explored the relationships between ecosystem patterns, structures and functions and drought, taking a typical climate change-sensitive area and an ecologically fragile area—the Yellow River Basin—as a case study. Drought assessment results revealed that the Yellow River Basin has experienced meteorological and hydrological drought during most of the last two decades, predominantly characterized by medium and slight droughts. The ecosystem patterns and structures changed dramatically as the grassland decreased and the landscape fragmentation index (F) increased with increasing wetness. The annual gross primary productivity (GPP) increased, the water use efficiency (WUE) declined and ecosystem service value (ESV) exhibited a W-shaped increase at the watershed scale, but there were significant regional differences. There were positive correlations between F, GPP, ESV and drought indices, while there was a negative correlation between WUE and drought indices at the watershed scale. Under drought stress, the ecosystem structure in the basin was disrupted, the GPP and ESV decreased, but the WUE increased. Notably, approximately 106 %, 20 %, and 1 % of the maximum reductions in F, GPP, and ESV, respectively, were caused by drought, while the maximum 4 % of WUE increased. Responses of some functions in the wetland and grassland to drought vary from those in other ecosystems. The mechanisms underlying ecosystem responses to drought were further investigated. This study enhances the understanding of these responses and will help stakeholders formulate drought mitigation policies and protect ecosystem health.

Spatiotemporal dynamics of ecological security pattern based on an optimized assessment framework and circuit theory: a case of Fuzhou City, China

The construction of the ecological security pattern (ESP) and ensuring its integrity are the focal points of ecological actions. However, existing evaluation frameworks lack comprehensiveness, and studies addressing temporal dimensions are limited. This study constructed a comprehensive framework to evaluate ESP and introduced a method to portray the relationship between ecological security status and landscape structure. Initially, multiple indicators were optimized to construct an ecological security index (ESI). The relationship between ESI and landscape structure was quantified. Then, fifteen spatiotemporal factors were combined using the Analytical Hierarchy Process to construct an ecological resistance layer. Finally, Circuit theory was applied to visualize trends in ESP. The findings indicated that ecological security was generally better but with a downward trend. The relationship between ESI and landscape fragmentation was mainly a tradeoff. The area of stable ecological sources was 1251.06 km2 (1996–2021). The number of minimal-cost corridors decreased continuously. Ecological sources with the highest conservation priority were the largest (1336.2 km2) in 2021. The number of pinch points was 25, 31, and 30, while ecological barriers covered 51.5, 148.74, and 139.51 km2 in 1996, 2008, and 2021, respectively. This study will serve as a valuable methodological reference and support ecological civilization projects in Fuzhou.

The Contribution of Cultivated and Semi-Natural Patches to the Beta Diversity of Nocturnal Lepidoptera within an Organic Century-Old Olive Grove in a Fragmented Landscape

The Contribution of Cultivated and Semi-Natural Patches to the Beta Diversity of Nocturnal Lepidoptera within an Organic Century-Old Olive Grove in a Fragmented Landscape

How Can Community Data Be Leveraged to Advance Primary Health Care? A Scoping Review of Community-Based Health Information Systems.

Community-based health information systems (CBISs) can provide critical insights into how community health systems function, and digitized CBISs may improve the quality of community-level data and facilitate integration and use of CBISs within the broader health system. This scoping review aims to understand how CBISs have been implemented, integrated, and used to support community health outcomes in low- and middle-income countries (LMICs). Both peer-reviewed and gray literature were included; relevant articles were identified using key terms and controlled vocabulary related to community/primary health care, health information systems, digital health, and LMICs. A total of 11,611 total records were identified from 5 databases and the gray literature. After deduplication, 6,985 peer-reviewed/gray literature were screened, and 95 articles/reports were included, reporting on 105 CBIS implementations across 38 countries. Findings show that 55% of CBISs included some level of digitization, with just 28% being fully digitized (for data collection and reporting). Data flow from the community level into the health system varied, with digitized CBISs more likely to reach national-level integration. National-level integration was primarily seen among vertical CBISs. Data quality challenges were present in both paper-based and digitized CBISs, exacerbated by fragmentation of the community health landscape with often parallel reporting systems. CBIS data use was constrained to mostly vertical and digitized (partially or fully) CBISs at national/subnational levels. Digitization can play a pivotal role in strengthening CBIS use, but findings demonstrate that CBISs are only as effective as the community health systems they are embedded within. Community-level data are often not being integrated into national/subnational health information systems, undermining the ability to understand what the community health needs are. Furthermore, stronger investments within community health systems need to be in place broadly to reduce fragmentation and provide stronger infrastructural and systemic support to the community health workforce.

Frugivores enhance potential carbon recovery in fragmented landscapes

Forest restoration is fundamental to overcoming biodiversity crises and climate change. In tropical forests, animals can improve forest recovery as they disperse >70% of tree species. However, representing animals in restoration and climate change policies remains challenging because a quantitative assessment of their contribution to forest and carbon recovery is lacking. Here we used individual-based models to assess frugivore-mediated seed rain in open areas along a fragmentation gradient. Movements of large birds were limited in landscapes with <40% forest cover, although small birds continued to disperse seeds. Large birds disperse seeds of late-successional species with higher carbon storage potential. Their restricted movement therefore reduced potential biomass in future forests by 38%. Maintaining forest cover >40% is essential to optimizing animals’ contribution to restoration success. Active restoration (for example, planting trees) is required in more fragmented landscapes to achieve carbon and biodiversity targets.

Digital capability gaps in traditional industries: influencing factors and strategic responses

ABSTRACT This paper introduces the concept of digital capability gaps (DCG); the relative difference of a firm’s ability to design and control products, services, and processes by utilising digital technologies in relation to customers and partners. These DCGs matter as they affect SME’s strategic responses and the value-creating relationships. Our findings reveal that strategic responses differ according to the magnitude of DCGs: Large DCGs may offer the opportunity for ‘digital diversification’ by using the capabilities developed to enter new domains. In cases of moderate DCGs, firms respond by adopting the role of a ‘digital parent’ and innovate on behalf of customers. Finally, in the cases of small DCGs, firms may respond by forming ‘digital collaborations.’ Our study also unpacks the influencing factors that affect DCGs: the consolidation or fragmentation of customer portfolio and competitive landscape and the extent to which players have a similar digital pressure for adopting digital capabilities.

Enhancing ecological network establishment with explicit species information and spatially coordinated optimization for supporting urban landscape planning and management

While insightful, ecological networks (ENs) incorporated in landscape planning and management may not provide a sufficient reference for maintaining biodiversity without explicit species information and coordinated actions, particularly in urban agglomeration areas. To address this gap, we conducted a study in Southern Jiangsu to refine the habitat ranges of 25 target species within the current ecological spaces by integrating explicit species information and diversified environmental variables using the MaxEnt model. Subsequently, an eco-space network and a habitat network were established for the region with identified connectivity hotspots as strategic areas for optimizing ENs. To assess the potential impacts of divergent actions during implementation, we developed four decision-making criteria from global and local views to simulate multiple optimization scenarios. These scenarios were quantitatively evaluated based on connectivity performance using cohesive metrics derived from complex network theory. Our findings demonstrated that incorporating species distribution and their interactions with the environment into ENs establishment can strengthen biodiversity conservation planning schemes within a limited range of regional ecological spaces. Furthermore, it highlighted shortcomings such as lack of focus on landscape priorities, discrepancies in corridors, and underestimation of landscape fragmentation caused by solely relying on an eco-space network-based approach. During optimization efforts, our results revealed that adopting a criterion focused on maximizing the number of strategic areas while adhering to a total area threshold can lead to a more connected and effective habitat network strategy when coordinating individual cities from a global view. This study provides valuable insights into prioritized landscapes and offers practical contributions toward mitigating biodiversity loss and improving urban sustainability.

Exploring Morphological Population Variability: Host Plant and Habitat Dependency in the Protected Moth Gortyna borelii (Lepidoptera, Noctuidae)

In this paper, we discuss the evolutionary implications of the correlation between different species of Peucedanum plants and the distribution of Gortyna borelii moth populations in Romania. We highlight geographic separation and isolation among these populations due to anthropogenic landscape fragmentation, which hinders genetic exchange. A geometric morphometric analysis was utilized to visualize and compare the morphometric variations in relation to the environmental variables, particularly the host plant. Additionally, the distribution of G. borelii populations across Europe and in Romania that are correlated with the host plant was analyzed. The significant morphological and morphometric differences between the analyzed populations support our working hypothesis, according to which the use of different Peucedanum species by the larvae of G. borelii leads to an intraspecific diversification correlated with the host plant species. The newly discovered population of G. borelii in Romania holds substantial conservation importance, necessitating protection measures, including demarcating habitat areas and raising awareness among stakeholders. G. borelii is a protected species at the European level (Habitats Directive 92/43/EEC, Appendices II and IV), considered endangered due to the isolation of its populations and anthropogenic pressures exerted through agricultural practices. Understanding the impact of agricultural practices on their habitat is crucial for effective management strategies. Overall, this study sheds light on the complex interplay between ecological adaptation, host plant specialization, and speciation dynamics in phytophagous insects, emphasizing the importance of conservation efforts to preserve G. borelii populations and their habitats.

Contribution of Protected Areas to Mitigate the Effect of Landscape Fragmentation in Slovakia

Abstract The main aim of the research is to identify landscape fragmentation (LF) in Slovakia with special emphasis on the contribution of protected areas (PAs) to mitigate the effect of LF. Results are presented in the final raster output (10 m grid). The raster contains 490,321,151 individual 10 m raster grids, with the LF average value of 59.12 % (where 0 represents fragmented landscape, 100 represents fully connected landscape by natural or semi-natural ecosystems) on the national level. Most of the territory of Slovakia falls within the range of values 55 – 65 %, which confirms the presence of significant continuous unfragmented areas. Based on the Ordinary Least Square (OLS) statistics results, there is a positive correlation of lower LF within the PAs network (p &lt; 0.05, Table 1) in comparison to the unprotected part of Slovakia. The results of geographically weight regression (GWR) proved a medium positive correlation (r2=0.36; r2adj=0.36; n=49,003), thus confirming to a certain extent the role of PAs in the mitigation of the effect of LF. On the other hand, the level of protection does not correlate significantly with fragmentation values, where a higher level of protection is not significantly connected with a lower level of LF. For each category of PA, individual statistics of quality and quantity of LF are estimated and subsequently compared with unprotected parts of Slovakia. The comparison of all PAs with each other resulted in 1,132 unique assessments. The overall average value of LF of unprotected parts is still rather high (56.42 %) and it shows that there are still significant areas existing, which are situated in unprotected parts of the country. Spatial analysis revealed, that these important parts are covering 93,065 hectares, and are variously spread across the whole of Slovakia. The average value of LF for these newly identified areas is 68.5 %. As output, the results of this research present a comprehensive national map of the level of LF and lists of PAs ranked according to the overall assessment of LF.