Fragmentation Events Research Articles

The origin of the endemic African grasshopper family Lentulidae (Orthoptera: Acridoidea) and its climate‐induced diversification

AbstractAimForest relicts in the mountainous regions of Africa represent one of the most diverse ecosystems on our planet, but the processes that have generated this remarkable diversity are still poorly understood. We estimate divergence times for an endemic, flightless grasshopper family and reconstruct a potential scenario for their colonization of Africa to test the hypothesis that the diversity of these mountain‐top endemics has been generated by multiple fragmentations and reconnections of tropical rain forests in parallel with climatic fluctuations.LocationSub‐Saharan Africa.TaxonLentulidae (Orthoptera).MethodsWe inferred the phylogeny of 7 genera and 28 species of the Lentulidae based on COI, 16S and Histone 3 sequences by using a Bayesian approach and we also estimated divergence dates. Based on our molecular phylogeny as well as the available information on the relationship of six additional genera and local occurrence records for 120 lentulid species across Africa, we reconstruct a potential colonization scenario for most species of this group.ResultsOur findings indicate that the forest‐bound lentulids of East Africa represent a monophyletic group that originates from South Africa. We show that major splits in the phylogeny of the Lentulidae coincide with three known fragmentation events of the African rain forests (27, 16 and 9 Ma) and that lentulids subsequently diversified rapidly in parallel with the aridification and strong geological activity in East Africa.Main conclusionsOur results corroborate the diversification patterns reported for several endemic African forest‐bound animal taxa at small scales and endemic African plant taxa at larger scales, highlighting the finding that much of the biodiversity presently found in the forest relicts of the Eastern Arc Mountains biodiversity hotspot has been generated by the interplay between humid periods that allowed the spread of forest‐bound lineages across Africa and periods of aridity‐driven isolation of forests and their associated fauna.

Using 10,000 Fragment Ions to Inform Scoring in Native Top-down Proteomics.

Protein fragmentation is a critical component of top-down proteomics, enabling gene-specific protein identification and full proteoform characterization. The factors that influence protein fragmentation include precursor charge, structure, and primary sequence, which have been explored extensively for collision-induced dissociation (CID). Recently, noticeable differences in CID-based fragmentation were reported for native versus denatured proteins, motivating the need for scoring metrics that are tailored specifically to native top-down mass spectrometry (nTDMS). To this end, position and intensity were tracked for 10,252 fragment ions produced by higher-energy collisional dissociation (HCD) of 159 native monomers and 70 complexes. We used published structural data to explore the relationship between fragmentation and protein topology and revealed that fragmentation events occur at a large range of relative residue solvent accessibility. Additionally, our analysis found that fragment ions at sites with an N-terminal aspartic acid or a C-terminal proline make up on average 40 and 27%, respectively, of the total matched fragment ion intensity in nTDMS. Percent intensity contributed by each amino acid was determined and converted into weights to (1) update the previously published C-score and (2) construct a native Fragmentation Propensity Score. Both scoring systems showed an improvement in protein identification or characterization in comparison to traditional methods and overall increased confidence in results with fewer matched fragment ions but with high probability nTDMS fragmentation patterns. Given the rise of nTDMS as a tool for structural mass spectrometry, we forward these scoring metrics as new methods to enhance analysis of nTDMS data.

Sea water shaping the freshwater biota: Hidden diversity and biogeographic history in the Paracanthocobitis zonalternans species complex (Teleostei: Nemacheilidae) in western Southeast Asia

Western Southeast Asia is hosting one of the world’s most diverse faunas, and one of the reasons for this huge diversity is the complex geologic past of the area, increasing the frequency of isolation and expansion events over evolutionary time scale. As an example case, the present study reveals the phylogeny and biogeographic history of the Paracanthocobitis zonalternans species complex, small benthic freshwater fish (Teleostei: Nemacheilidae) that are commonly occurring across western Southeast Asia (from central Myanmar through western and southern Thailand to northern Malaysia). The group is particularly interesting since it occurs in three biogeographic subdivisions (Indian, Indochinese, Malay/Sundaic) and across all of the major biogeographic barriers in the region. Basing on mitochondrial and nuclear sequence data of 93 samples from about 50 localities we found six major clades, most with exclusive geographic distribution. Divergence time dated the origin of the P. zonalternans species complex to early Miocene (17.8 MYA) and a biogeographic analysis identified the Tenasserim region as the ancestral region. From this region the fish spread during periods of lowered global sea level, particularly during late Miocene (11-8 MYA) northwards into all Burmese river basins and southwards into south Thailand and northern Malaysia. Besides lowered global sea level periods, local stream capture events allowed the complex to expand, e.g. into the Mae Klong basin. Strong fragmentations during periods with elevated sea level during the Pliocene and Pleistocene repeatedly restricted populations to refuges and shaped the observed major lineages. Our results document a higher diversity within the P. zonalternans species complex than formerly believed and a strong impact of global sea level on its evolutionary history. Low sea levels promoted dispersal and elevated sea levels fragmentation events. A very similar impact of sea level changes can be expected in all stationary fauna (freshwater and terrestrial) in all non-mountainous coastal regions worldwide.

In situ observation of the percolation threshold in multiphase magma analogues

Magmas vesiculate during ascent, producing complex interconnected pore networks, which can act as outgassing pathways and then deflate or compact to volcanic plugs. Similarly, in-conduit fragmentation events during dome-forming eruptions create open systems transiently, before welding causes pore sealing. The percolation threshold is the first-order transition between closed- and open-system degassing dynamics. Here, we use time-resolved, synchrotron-source X-ray tomography to image synthetic magmas that go through cycles of opening and closing, to constrain the percolation threshold ΦC at a range of melt crystallinity, viscosity and overpressure pertinent to shallow magma ascent. During vesiculation, we observed different percolative regimes for the same initial bulk crystallinity depending on melt viscosity and gas overpressure. At high viscosity (> 106 Pa s) and high overpressure (~ 1–4 MPa), we found that a brittle-viscous regime dominates in which brittle rupture allows system-spanning coalescence at a low percolation threshold (ΦC~0.17) via the formation of fracture-like bubble chains. Percolation was followed by outgassing and bubble collapse causing densification and isolation of the bubble network, resulting in a hysteresis in the evolution of connectivity with porosity. At low melt viscosity and overpressure, we observed a viscous regime with much higher percolation threshold (ΦC > 0.37) due to spherical bubble growth and lower degree of crystal connection. Finally, our results also show that sintering of crystal-free and crystal-bearing magma analogues is characterised by low percolation thresholds (ΦC = 0.04 – 0.10). We conclude that the presence of crystals lowers the percolation threshold during vesiculation and may promote outgassing in shallow, crystal-rich magma at initial stages of Vulcanian and Strombolian eruptions.

Phenomics-Based Quantification of CRISPR-Induced Mosaicism in Zebrafish.

Genetic mosaicism can manifest as spatially variable phenotypes that vary from site to site within an organism. Here, we use imaging-based phenomics to quantitate phenotypes at many sites within the axial skeleton of CRISPR-edited G0 zebrafish. Through characterization of loss-of-function cell clusters in the developing skeleton, we identify a distinctive size distribution shown to arise from clonal fragmentation and merger events. We quantitate the phenotypic mosaicism produced by somatic mutations of two genes, plod2 and bmp1a, implicated in human osteogenesis imperfecta. Comparison of somatic, CRISPR-generated G0 mutants to homozygous germline mutants reveals phenotypic convergence, suggesting that CRISPR screens of G0 animals can faithfully recapitulate the biology of inbred disease models. We describe statistical frameworks for phenomic analysis of spatial phenotypic variation present in somatic G0 mutants. In sum, this study defines an approach for decoding spatially variable phenotypes generated during CRISPR-based screens.

Discrete fragmentation systems in weighted pmb {ell ^{1}} spaces

We investigate an infinite, linear system of ordinary differential equations that models the evolution of fragmenting clusters. We assume that each cluster is composed of identical units (monomers), and we allow mass to be lost, gained or conserved during each fragmentation event. By formulating the initial-value problem for the system as an abstract Cauchy problem (ACP), posed in an appropriate weighted ell ^1 space, and then applying perturbation results from the theory of operator semigroups, we prove the existence and uniqueness of physically relevant, classical solutions for a wide class of initial cluster distributions. Additionally, we establish that it is always possible to identify a weighted ell ^1 space on which the fragmentation semigroup is analytic, which immediately implies that the corresponding ACP is well posed for any initial distribution belonging to this particular space. We also investigate the asymptotic behaviour of solutions and show that, under appropriate restrictions on the fragmentation coefficients, solutions display the expected long-term behaviour of converging to a purely monomeric steady state. Moreover, when the fragmentation semigroup is analytic, solutions are shown to decay to this steady state at an explicitly defined exponential rate.

NOTCH3 is non-enzymatically fragmented in inherited cerebral small-vessel disease

The small-vessel disorder cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) arises from mutations in the human gene encoding NOTCH3 and results in vascular smooth muscle cell degeneration, stroke, and dementia. However, the structural changes in NOTCH3 involved in CADASIL etiology are unclear. Here, we discovered site-specific fragmentation of NOTCH3 protein in pathologically affected vessels of human CADASIL-affected brains. EM-based experiments to pinpoint NOTCH3 localization in these brains indicated accumulation of NOTCH3 fragmentation products in the basement membrane, collagen fibers, and granular osmiophilic material within the cerebrovasculature. Using antibodies generated against a disease-linked neo-epitope found in degenerating vascular medium of CADASIL brains, we mapped the site of fragmentation to the NOTCH3 N terminus at the peptide bond joining Asp80 and Pro81 Cleavage at this site was predicted to separate the first epidermal growth factor (EGF)-like domain from the remainder of the protein. We found that the cleavage product from this fragmentation event is released into the conditioned medium of cells expressing recombinant NOTCH3 fragments. Mutagenesis of Pro81 abolished the fragmentation, and low pH and reducing conditions enhanced NOTCH3 proteolysis. Furthermore, substitution of multiple cysteine residues of the NOTCH3 N terminus activated proteolytic release of the first EGF-like repeat, suggesting that the elimination of multiple disulfide bonds in NOTCH3 accelerates its fragmentation. These characteristics link the signature molecular genetic alterations present in individuals with CADASIL to a post-translational protein alteration in degenerating brain arteries. The cellular consequences of these pathological NOTCH3 fragments are an important area for future investigation.

Cryptic Speciation of a Deep-Sea Demersal Fish of the Genus Bothrocara in the Japan Sea.

Deep-sea demersal fishes of the Bothrocara hollandi species complex are distributed in the Japan Sea, the Okhotsk Sea, and the northwestern Pacific Ocean. Based on the nucleotide sequences of the nuclear Internal Transcribed Spacer 1 (ITS1) region and the microsatellite analysis, cryptic speciation resulting in the existence of two species (sp. 1 and sp. 2) in the Japan Sea was indicated for this species complex. In the Japan Sea off the San-in district, the westernmost part of the Japanese mainland, the frequency of sp. 2 individuals was highest at a depth of ∼400 m and decreased at both greater and lesser depths. Complete genetic deviation was observed between the individuals of the Japan Sea and the other sea areas, with the exception of a single sp. 2 individual, which shared an ITS1 sequence with an individual from the Pacific Ocean. Furthermore, a microsatellite analysis showed that the individuals of the other sea areas were more closely related to sp. 2 individuals. Two species were thought to have deviated from each other after their isolation from the individuals of the sea areas outside of the Japan Sea, through the occurrence of habitat fragmentation and bottleneck events in the Japan Sea during the glacial periods. Group A, one of two mitochondrial haplotype groups that were reported for the Japan Sea individuals in the previous studies, may have evolved within the lineages of sp. 2.

Investigating the CO trimer geometry using Coulomb imaging technique

SynopsisThe Coulombic explosion of multi-charged (CO)3q+ molecular ions produced in low energy collisions with Ar9+ projectiles was investigated using recoil ion momentum spectrometry (RIMS). A preliminary analysis of the data clearly shows a dominant triangular cyclic structure. Events that may correspond to a linear chain are also observed, but the latter could as well originate from false coincidences, associated to the fragmentation of dimers. A careful analysis of this source of background is proposed to disentangle trimer fragmentation events from false coincidences.

Fragility and an extremely low shear modulus of high porosity silicic magma

The rheology and strength of bubbly magma govern eruption dynamics by determining the possibility of fragmentation of ascending magmas. They are also required parameters for understanding seismic monitoring. We measured the rheology and strength of high porosity rhyolitic magma at 500–950 °C. The measured shear modulus and strength are several orders of magnitude lower than bubble-free rhyolite melt, implying that high porosity magma cannot avoid fracturing during magma ascent. The occurrence of fractures is observed in the low-temperature magma (≤800 °C). In this temperature range, the measured attenuation is low. That is, the elastic energy originated by deformations avoids attenuation and is stored in the bubbly magma until released by fracturing (Q > 1). The newly found porosity-dependent strength based on our measurements comprehensively explains three different fragmentation criteria that have been previously proposed independently. Our measurements also show that the shear modulus becomes lower by increasing porosity, which can slow the shear wave velocity. These results suggest that knowing the attenuation of the seismic wave is useful to evaluate magma temperature and the possibility of a fragmentation event that may determine subsequent volcanic activities.

A hybrid approach to improve microstructure and mechanical properties of cold spray additively manufactured A380 aluminum composites

This study comprehensively determined the microstructure and mechanical properties of cold spray additively manufactured A380 alloy components. In this regard, three types of Al2O3/A380 composite deposits were prepared by separately mixing spherical, irregular, and spherical + irregular shaped Al2O3 particulates with the original A380 alloy powder. The as-sprayed deposits were subsequently subjected to heat-treatment at 350 °C for 4 h. The effects of Al2O3 morphology on the microstructure and mechanical properties of A380 deposits were systematically investigated at different processing stages by electron backscattered diffraction analysis and tensile testing, respectively. Moreover, the evolution of defects (pores and dislocation) inside the deposits was comprehensively studied using X-ray computed tomography and transmission electron microscopy techniques. The results indicated that the deposit containing spherical Al2O3 exhibited significantly reduced porosity, grain refinement and a significant increase in strength (~70%) in contrast to the pure A380 alloy deposit. This could be attributed to the tamping effect of the spherical Al2O3 particles. In contrast, the irregular Al2O3 particles primarily indicated the embedding effect with recurrence of fragmentation events inside the deposit. Consequently, the deposit containing irregular Al2O3 indicated only a slight decrease in porosity and a ~40% increase in strength in contrast to the pure A380 alloy deposit. The tensile test results indicated that heat-treatment significantly improved the plasticity of deposits through fusion of nano-sized pores, reduction in dislocation density and static re-crystallization. Therefore, the hybrid approach of adding a suitable tamping aid to the original A380 alloy powder and post-spray heat-treatment optimized the microstructure of cold spray additively manufactured components to significantly improve their mechanical properties.

Physical Characterization of the 2017 December Outburst of the Centaur 174P/Echeclus

Abstract The Centaurs are the small solar system bodies intermediate between the active inner solar system Jupiter family comets and their inactive progenitors in the trans-Neptunian region. Among the fraction of Centaurs which show comet-like activity, 174P/Echeclus is best known for its massive 2005 outburst in which a large apparently active fragment was ejected above the escape velocity from the primary nucleus. We present visible imaging and near-infrared spectroscopy of Echeclus during the first week after its 2017 December outburst taken at the Faulkes North and South Telescopes and the NASA IRTF, the largest outburst since 2005. The coma was seen to be highly asymmetric. A secondary peak was seen in the near-infrared 2D spectra, which is strongly hinted at in the visible images, moving hyperbolically with respect to the nucleus. The retrieved reflectance spectrum of Echelcus is consistent with the unobscured nucleus but becomes bluer when a wider extraction aperture is used. We find that Echeclus’s coma is best explained as dominated by large blue dust grains, which agrees with previous work. We also conducted a high-resolution orbital integration of Echeclus’s recent evolution and found no large orbital changes that could drive its modern evolution. We interpret the second peak in the visible and near-infrared data sets as a large cloud of larger-than-dust debris ejected at the time of outburst. If Echeclus is typical of the Centaurs, there may be several debris ejection or fragmentation events per year on other Centaurs that are going unnoticed.

Interactions between asteroid fragments during atmospheric entry

The current work explores the interactions between asteroid fragments and the associated flow topology to motivate a physically consistent representation of the fragmentation process following a fragmentation event during atmospheric entry. Multibody aerodynamic simulations run with computational fluid dynamics (CFD) solvers were used to generate a lookup table of forces detailing the interactions of two spheres. Trajectory simulations parsing the resulting database to determine the relative motions of any two spherical fragments were then validated with hypersonic wind tunnel experiments. A following series of fragment interaction simulations yielded categorization of the fragments' final relative states and an estimate of the total time of interaction. The fragment interaction model was nondimensionalized to permit study over a wide range of possible asteroid impacts. The interaction parameters are presented with explicit semi-analytic equations, defining the asteroid fragment-flow interaction model and thereby eliminating the need to perform a separate fragment interaction simulation for each fragmentation event in an atmospheric entry model. Finally, a set of illustrative examples demonstrates the efficacy of the model in a variety of fragmentation situations.

Jamming and tiling in fragmentation of rectangles.

We investigate a stochastic process where a rectangle breaks into smaller rectangles through a series of horizontal and vertical fragmentation events. We focus on the case where both the vertical size and the horizontal size of a rectangle are discrete variables. Because of this constraint, the system reaches a jammed state where all rectangles are sticks, that is, rectangles with minimal width. Sticks are frozen as they cannot break any further. The average number of sticks in the jammed state, S, grows as S≃A/sqrt[2πlnA] with rectangle area A in the large-area limit, and remarkably, this behavior is independent of the aspect ratio. The distribution of stick length has a power-law tail, and further, its moments are characterized by a nonlinear spectrum of scaling exponents. We also study an asymmetric breakage process where vertical and horizontal fragmentation events are realized with different probabilities. In this case, there is a phase transition between a weakly asymmetric phase where the length distribution is independent of system size and a strongly asymmetric phase where this distribution depends on system size.

Comparison of 3-D Raytracing and Finite Frequency Tomography

We performed 3-D seismic tomography using teleseismic arrival time at Southwest Mexico. The Mexican subduction zone results from successive fragmentation events that affected the ancient Farallon plate as various segments of the East Pacific rise approached the paleo-trench off western North America. The complexity in this region is related to two subducting oceanic plates, the Rivera and Cocos plates, that have different ages, compositions, convergence velocities and subduction dip angles. In this study, we compared the 3-D raytracing tomography model with finite frequency tomography model. Final models show the differences in amplitude and pattern between the raytracing and finite frequency. 3D raytracing models produced sharper images of fast velocity structures in the mantle. The deeper slabs are more coherent and show less broadening with depth than using 1D finite frequency kernels. However, although the finite frequency and 3-D ray tracing models show some differences in amplitude and pattern, the overall agreement of the models supports the interpretation of Yang et al. (2009) that slab rollback is occurring in South Western Mexico. One possible different interpretation between the raytracing and finite frequency theory results concerns the deep structure of the Rivera slab. The finite frequency models show that the Rivera slab is clearly observable at a depth of about 300km but fades away at greater depths. However, the 3-D ray tracing model shows a clear fast velocity band down to a depth of 400 km and thus our model does not support a slab tear of the Rivera plate above 400 km depth

High-throughput mass spectrometry scheme for screening and quantification of flavonoids in antioxidant nutraceuticals

Antioxidant nutraceuticals functional characteristic science is a challenging field for combining sensitivity and comprehensiveness. A untargeted screening and quantification method based on ultra-high performance liquid chromatography coupled to Quadrupole-Orbitrap high resolution mass spectrometry has been developed for determination of multiple classes of flavonoids in eight-three nutraceuticals samples. The data acquisition is based on a non-target approach of sequential full scan and variable data independent acquisition of twenty consecutive fragmentation events. The flavonoids include flavanols, flavones, flavanones, anthocyanidins, flavonols and isoflavones. A processing strategy is introduced to implementing filtering methods based on data feature extraction, common ion selection, shoulder peak removal, response threshold adjustment, mass shift and characteristic structural fragments evaluation. Confirmation is based on both accurate mass and isotopic assignment of standards, and further quantification is achieved by fragmentation. This scheme allows in depth characterization of flavonoids with the entire fragments.

Gold(I) Cationization Promotes Ring Opening in Lysine-Containing Cyclic Peptides.

A strategy to sequence lysine-containing cyclic peptides by MSn is presented. Doubly protonated cyclic peptides ions are transformed into gold (I) cationized peptide ions via cation switching ion/ion reaction. Gold(I) cationization facilitates the oxidation of neutral lysine residues in the gas phase, weakening the adjacent amide bond. Upon activation, facile cleavage N-terminal to the oxidized lysine residue provides a site-specific ring opening pathway that converts cyclic peptides into acyclic analogs. The ensuing ion contains a cyclic imine as the new N-terminus and an oxazolone, or structural equivalent, as the new C-terminus. Product ions are formed from subsequent fragmentation events of the linearized peptide ion. Such an approach simplifies MS/MS data interpretation as a series of fragment ions with common N- and C-termini are generated. Results are presented for two cyclic peptides, sunflower trypsin inhibitor and the model cyclic peptide, β-Loop. The power of this strategy lies in the ability to generate the oxidized peptide, which is easily identified via the loss of HAuNH3 from [M + Au]+. While some competitive processes are observed, the site of ring opening can be pinpointed to the lysine residue upon MS4 enabling the unambiguous sequencing of cyclic peptides.

Kinetic regimes in aggregating systems with spontaneous and collisional fragmentation

We analyze systems of clusters and interacting upon colliding—a collision between two clusters may lead to merging or fragmentation—and we also investigate the influence of additional spontaneous fragmentation events. We consider both closed systems in which the total mass remains constant and open systems driven by a source of small-mass clusters. In closed systems, the size distribution of aggregates approaches a steady state. For these systems the relaxation time and the steady state distribution are determined mostly by spontaneous fragmentation while collisional fragmentation plays a minor role. For open systems, in contrast, the collisional fragmentation dominates. In this case, the system relaxes to a quasi-stationary state where cluster densities linearly grow with time, while the functional form of the cluster size distribution persists and coincides with the steady state size distribution of a system which has the same aggregation and fragmentation rates and only collisional fragmentation.

Lowland tapirs facilitate seed dispersal in degraded Amazonian forests

AbstractThe forests of southeastern Amazonia are highly threatened by disturbances such as fragmentation, understory fires, and extreme climatic events. Large‐bodied frugivores such as the lowland tapir (Tapirus terrestris) have the potential to offset this process, supporting natural forest regeneration by dispersing a variety of seeds over long distances to disturbed forests. However, we know little about their effectiveness as seed dispersers in degraded forest landscapes. Here, we investigate the seed dispersal function of lowland tapirs in Amazonian forests subject to a range of human (fire and fragmentation) and natural (extreme droughts and windstorms) disturbances, using a combination of field observations, camera traps, and light detection and ranging (LiDAR) data. Tapirs travel and defecate more often in degraded forests, dispersing much more seeds in these areas [9,822 seeds per ha/year (CI95% = 9,106; 11,838)] than in undisturbed forests [2,950 seeds per ha/year (CI95% = 2,961; 3,771)]. By effectively dispersing seeds across disturbed forests, tapirs may contribute to natural forest regeneration—the cheapest and usually the most feasible way to achieve large‐scale restoration of tropical forests. Through the dispersal of large‐seeded species that eventually become large trees, such frugivores also contribute indirectly to maintaining forest carbon stocks. These functions may be critical in helping tropical countries to achieve their goals to maintain and restore biodiversity and its ecosystem services. Ultimately, preserving these animals along with their habitats may help in the process of natural recovery of degraded forests throughout the tropics.Abstract in Portuguese is available with online material.

Asteroid cratering families: recognition and collisional interpretation

Aims.We continue our investigation of the bulk properties of asteroid dynamical families identified using only asteroid proper elements to provide plausible collisional interpretations. We focus on cratering families consisting of a substantial parent body and many small fragments.Methods.We propose a quantitative definition of cratering families based on the fraction in volume of the fragments with respect to the parent body; fragmentation families are above this empirical boundary. We assess the compositional homogeneity of the families and their shape in proper element space by computing the differences of the proper elements of the fragments with respect to the ones of the major body, looking for anomalous asymmetries produced either by post-formation dynamical evolution, or by multiple collisional/cratering events, or by a failure of the hierarchical clustering method (HCM) for family identification.Results.We identified a total of 25 dynamical families with more than 100 members ranging from moderate to heavy cratering. For three families (4, 15 and 283) we confirm the occurrence of two separate cratering events, while family (569) Misa is a mixed case, with one cratering event and one fragmentation event. The case of family 3 remains dubious, in that there could be either one or two collisions. For family 20, we propose a double collision origin, not previously identified. In four cases (31, 480, 163 and 179) we performed a dedicated search for dynamical resonant transport mechanisms that could have substantially changed the shape of the family. By using a new synthetic method for computation of secular frequencies, we found possible solutions for families 31, 480, and 163, but not for family 179, for which we propose a new interpretation, based on a secular resonance contaminating this family: the family of 179 should be split into two separate clusters, one containing (179) itself and the other, family (9506) Telramund, of fragmentation type, for which we have computed an age.