Fragmented Approach Research Articles

Challenges of environmental impact assessment in addressing environmental risk: insights from Brazilian practice in mining dams

ABSTRACT Environmental Impact Assessment (EIA) and Risk Assessment (RA) are critical tools for assessing and managing environmental impacts and risks of projects. However, their integration remains a challenge. This study examines how EIA practices account for risks in evaluating the environmental feasibility of mining dam projects in the state of Minas Gerais, Brazil. We analyzed licensing processes for projects approved between 2004 and 2022, using data from the state’s environmental agency. Methods included documentary and content analysis of Environmental Impact Statements (EIS) and review reports. Among the 25 cases analyzed, most focused solely on risk control without performing RA. Only two cases included RA, and two others lacked any risk-related information. Review reports revealed that only two went beyond considering risk control when assessing environmental feasibility. This suggests decision-making relies on a narrow focus on risk control. Furthermore, there is a misalignment between the EIS content and the criteria for project acceptability in review reports. Finally, we explore how politicization may undermine the EIA’s role in objective, and evidence-based decision-making. These findings underscore the superficial and fragmented approach to addressing environmental risk in mining dam assessments in Minas Gerais.

Liability and Compensation for Damage in Connection with the Carriage of Hazardous and Noxious Substances by Sea under Croatian Law

The quantity and volume of hazardous and noxious substances transported by ships as cargo have been increasing markedly. The trend is anticipated to persist and even accelerate in the Adriatic Sea due to the expansion of port infrastructure. Concomitantly with this expansion, the probability of incidents involving these substances, which have the potential to cause significant damage, is also on the rise. At present, the Republic of Croatia lacks the specific legal framework that would provide a comprehensive legal basis for liability, prompt, and adequate compensation for damage to persons and property, liability for clean-up, and reinstatement measure costs, and economic losses resulting from the transportation of such substances by sea. The objective of this paper is to provide an overview of the provisions of Croatian law that regulate liability and compensation in the event of such an accident. The analysis demonstrates the deficiencies of fragmented approach. Moreover, the paper examines the stipulations of the International Convention on Liability and Compensation for Damage in Connection with the Carriage of Hazardous and Noxious Substances by Sea of 2010, and the prospective advantages of its ratification. These include double-tier liability, a special regime of limitation of liability, compulsory insurance of liability and the right to direct action. In consideration of Croatia's maritime and tourist-oriented economy, the authors highlight the country's substantial interest in not only the ratification of the 2010 HNS Convention and paving the way to its entry into force, but also in amendments to the Croatian Maritime Code in that respect.

An Update on Australian Policy around Lamb Marking with Examination of Potential Drivers. Comment on Johnston et al. How Well Does Australian Animal Welfare Policy Reflect Scientific Evidence: A Case Study Approach Based on Lamb Marking. Animals 2023, 13, 1358.

This commentary provides an update and clarification on the legislative landscape surrounding mulesing in Australia since the publication of the 2023 study, "How Well Does Australian Animal Welfare Policy Reflect Scientific Evidence: A Case Study Approach Based on Lamb Marking". The article explores legislative changes mandating the use of pain relief for mulesing in various states, emphasising Victoria's original role, and highlighting the fragmented state-based legislative approach to animal welfare. It discusses the impact of these legislative changes on industry practices and animal welfare outcomes. The commentary highlights the complexities of policy development in this area, due in part to the diverse and often conflicting interests of stakeholders and the public. It underscores the importance of transparency, stakeholder collaboration, and scientifically informed policymaking to effectively enhance animal welfare standards.

Deciphering Stereoselectivity in Hurd-Claisen Rearrangements: A Comprehensive Study of Electrostatic Interactions from Shubin's Energy Decomposition Analysis.

In this study, we explore the stereoselectivity of Hurd-Claisen Rearrangements, focusing on the influence of two electron-withdrawing groups and eight diverse substituents. Utilizing the Curtin-Hammett principle, we performed energy calculations for reactions, products, and transition states using the M062X/def2TZVPP compound model. Our analysis reveals that kinetic factors predominantly dictate the reaction equilibrium. A key aspect of our research is the application of Shubin's energy decomposition analysis to optimized transition states, highlighting the significant role of electrostatic interactions in determining stereoselectivity. We further dissected each transition state into four fragments: the electron-withdrawing groups ( , ), the Hurd group ( ), various substituents ( , , , , , , , ), and the central fragment. This fragmentation approach enabled an in-depth analysis of group dipole moments, providing insights into the electrostatic forces at play. Our findings shed light on the intricate mechanisms driving stereoselectivity in Hurd-Claisen Rearrangements and enhance the understanding of molecular interactions, offering valuable implications for organic synthesis.

Quantitative evaluation of noncovalent interactions with negative fragmentation approach including basis set superposition error correction

Abstract We propose a negative fragmentation approach (NFA), including counterpoise (CP) correction to basis set superposition error (BSSE) for quantitatively evaluating intra- and intermolecular noncovalent interactions. Noncovalent interactions are widely found in chemistry and biology and are regarded as essential interactions. However, there are few general methods for evaluating these individual intra- and intermolecular interaction energies because of two issues: (i) difficulty in the evaluation of intramolecular interactions due to the interacting sites connected with covalent bonds and (ii) BSSE affecting the quantitative accuracy of interaction analysis. In our scheme, we overcome the issue (i) using the NFA scheme, which can evaluate intra- and intermolecular interactions as a fragment–fragment interaction of interacting sites, and address the issue (ii) using the CP method. Here, NFA including the CP correction was applied to various molecular systems, providing comparable results for intermolecular interactions to supermolecule calculations with the CP correction and also succeeding in the evaluation of intramolecular interactions and its BSSEs. It is notable that our NFA scheme does not require any particular program or a modification of the program codes. These indicate that many researchers can apply our NFA scheme to various molecular systems.

An organisation working mainly reactively instead of proactively: a qualitative study of how frail users of home care services and their next of kin experience crises

BackgroundFrail people receiving home care services face an increased risk of developing crisis, which can result in adverse events, coercive measures, and acute institutionalisation. The prevalence of frailty is expected to increase due to the ageing population in most countries. However, our knowledge of the process leading to crises among frail community-dwelling patients remains limited. The aim of our study was to explore how users of home care services and their next of kin experienced crises and how these crises were approached by home care services.MethodsA qualitative explorative design with 21 interviews was conducted. We explored crises within the last year that had led to an acute institutionalisation (hospital or nursing home) or to an unstable situation with high risk of institutionalisation. Systematic text condensation (STC) was used to analyse the data.ResultsOur findings are summarised into one overarching theme; an organisation working mainly reactively instead of proactively, which is supported by four subthemes: (1) insufficient communication—a determinant of crises, (2) the lack of a holistic approach, (3) a sense of being a burden, and (4) the complexity of crises. The reactive approach is demonstrated in the participant’s experience of insufficient communication and the lack of a holistic approach from the service, but also in the user’s sense of being a burden, which seems to be reinforced by the experienced busyness from the staff in the home care services. This reactive approach to crises seems to have contributed to difficulties in detecting the various stressors involved in the complex process leading to crisis.ConclusionsOur findings suggest that home care services tend to be characterised by a reactive approach to rising instability and the development of crises for users. This can be interpreted as an emergent property of the organisation and the adaptation towards exceeding demands due to insufficient capacity in health care services. We recommend the use of multicomponent care programmes comprising interdisciplinary case conferences in home care services to implement a cultural change that can shift the service from a reactive, fragmented, and task-oriented approach to a more proactive approach.

Digital Twins and Civil Engineering Phases: Reorienting Adoption Strategies

Abstract Digital twin (DT) technology has received immense attention over the years due to the promises it presents to various stakeholders in science and engineering. As a result, different thematic areas of DT have been explored. This is no different in specific fields such as manufacturing, automation, oil and gas, and civil engineering, leading to fragmented approaches for field-specific applications. The civil engineering industry is further disadvantaged in this regard as it relies on external techniques by other engineering fields for its DT adoption. A rising consequence of these extensions is a concentrated application of DT to the operations and maintenance phase. On another spectrum, building information modeling (BIM) is pervasively utilized in the planning/design phase, and the transient nature of the construction phase remains a challenge for its DT adoption. In this article, we present a phase-based development of DT in the architecture, engineering, and construction industry. We commence by presenting succinct expositions on DT as a concept and as a service, and establish a five-level scale system. Furthermore, we present separately a systematic literature review of the conventional techniques employed at each civil engineering phase. In this regard, we identified enabling technologies such as computer vision for extended sensing and the Internet of things for reliable integration. Ultimately, we attempt to reveal DT as an important tool across the entire life cycle of civil engineering projects, and nudge researchers to think more holistically in their quest for the integration of DT for civil engineering applications.

Effects of Low-Dose X-Ray Exposure on Hematological Parameters In Vivo

Background: High levels of IR are known to induce cancer and disrupt biological processes through oxidative damage primarily driven by reactive oxygen species (ROS) overproduction. This study aims to investigate the effects of low-dose X-ray exposure on Hematologic parameters in Wistar albino rats, contributing to the limited literature on low-dose X-ray exposure and its impacts on liver health. Methods: Adult male Wistar albino rats, averaging 150 ± 25 g, were divided into four experimental groups. The rats underwent irradiation using a 6 MV linear accelerator, and blood samples were collected for biochemical analysis. Hematologic parameters, including Red Blood Cells (RBCs), platelets (PLTs), hemoglobin (Hb), white blood cells (WBCs), lymphocytes (LY), and granulocytes (GRAN), were assessed. Results: Significant variations in hematologic parameters were observed based on the method and dosage of radiation. The RBC count decreased from 9.15 ± 0.21 (control) to 3.32 ± 0.25 (higher dosage), a reduction of 58.93%. Platelet counts dropped by 51.43% and 32.46% for single and fragmented dosage approaches, respectively. Moreover, WBC and granulocyte counts were significantly reduced, with hemoglobin levels declining by 47.52% and 57.34% following specific irradiation rates (p < 0.001). Conclusion: The findings underscore the adverse effects of ionizing radiation on hematologic parameters, indicating that low-dose X-ray exposure does not promote an adaptive response in hematology when followed by higher radiation doses.

Economic and Management Sciences Teachers’ Experiences of Teaching Financial Literacy: Implications for Teacher Training

This paper reports on economic and management sciences (EMS) teachers’ experiences of teaching the financial literacy component in this learning area. It also provides a holistic understanding of their challenges and professional development needs in this integrated field. A qualitative case study approach was used to allow for an in-depth understanding of EMS teachers’ views and experiences. Data were collected through open-ended questionnaires and semi-structured interviews. Purposive sampling was used to select information-rich participants from selected government secondary schools in Gauteng province, South Africa. Participants in the study included grades 8 and 9 EMS teachers. A thematic analysis approach was used to analyze data collected from the open-ended questionnaires and interview responses. The findings show that, due to poor training, EMS teachers continue to experience challenges in integrating the financial literacy component. Consequently, teachers default to fragmented approaches to teaching EMS, which undermines government initiatives aimed at realizing social transformation objectives through the EMS curriculum. The study recommends an implicit approach to teacher development programs by incorporating EMS teachers’ views, experiences, and specific needs as well as contexts. The research contributes to the literature on commerce education in South Africa by offering ways in which education can be transformative and how the quality of teaching financial literacy in EMS can improve.

Excited State Bond Homolysis of Vanadium(V) Photocatalysts for Alkoxy Radical Generation.

Advancements in photocatalysis have transformed synthetic organic chemistry, using light as a powerful tool to drive selective chemical transformations. Recent approaches have focused on metal-halide ligand-to-metal charge transfer (LMCT) photoactivated bond homolysis reactions leveraged by earth-abundant elements to generate valuable synthons for radical-mediated cross-coupling reactions. Of recent utility, oxovanadium(V) LMCT photocatalysts exhibit selective alkoxy radical generation from aliphatic alcohols upon blue light (UVA) irradiation under mild conditions. The selective photochemical liberation of alkoxy radicals is valuable for applying late-stage fragmentation approaches in organic synthesis and depolymerization strategies for nonbiodegradable polymers. Steady-state and time-resolved spectroscopy were used to assign the electronic structure of three well-defined V(V) photocatalysts in their ground and excited states. We assign the excited state for this transformation at earth-abundant vanadium(V), interrogating the electronic structure using static UV-visible absorption, ultrafast transient absorption, and electron paramagnetic resonance spectroscopy coupled to computational approaches. These findings afford assignments of the short-lived excited state intermediates that dictate selective homolytic bond cleavage in metal alkoxides, illustrating the valuable insight gleaned from fundamental investigations of the molecular photochemistry responsible for light-escalated chemical transformations.

Формування інноваційної екосистеми сталого економічного відновлення і розвитку регіонів для посилення національної безпеки україни

This article delves into establishing an innovative ecosystem in Ukraine, essential for sustainable economic recovery and regional development. The significance of innovation in driving economic growth cannot be overstated, especially in a country that has faced numerous challenges, including political instability, economic downturns, and external threats. An innovative ecosystem serves as a framework that integrates various stakeholders, including scientific institutions, higher education establishments, businesses, and government entities, to foster collaboration and create an environment conducive to innovation. The article begins by defining an innovative ecosystem and its core components. It highlights the importance of synergy among different participants in the innovation process. Scientific institutions are crucial for research and development (R&D), providing the necessary knowledge and expertise to drive innovation. Higher education institutions play a pivotal role in preparing a skilled workforce equipped with the necessary competencies to meet the demands of a rapidly changing labor market. On the other hand, businesses are responsible for commercializing innovations and bringing new products and services to market. One of the critical aspects discussed is the need for effective collaboration among these stakeholders. The article emphasizes that strong partnerships between academia and industry characterize thriving innovative ecosystems. This collaboration can take various forms, including joint research projects, internships for students in companies, and knowledge transfer initiatives. These entities can leverage each other’s strengths to accelerate the innovation process by working together. Moreover, the article addresses the barriers to forming an innovative ecosystem in Ukraine. These barriers include inadequate regulatory frameworks supporting innovation, insufficient funding for R&D activities, and a fragmented approach to innovation policy. Ukraine’s current state of innovation is often hindered by bureaucratic obstacles that slow the implementation of innovative solutions. Therefore, policymakers must create a supportive environment that encourages investment in research and development. In addition to addressing systemic challenges, the article highlights the importance of fostering a culture of innovation within society. This includes promoting entrepreneurship and encouraging individuals to pursue innovative ideas. Educational programs focused on entrepreneurship can inspire young people to participate actively in the innovation ecosystem. Furthermore, public awareness campaigns can help shift societal attitudes towards risk-taking and experimentation. The conclusion of the article emphasizes Ukraine’s potential to create a robust, innovative ecosystem that can enhance its competitiveness on the global stage. By leveraging its human capital, rich natural resources, and growing technological capabilities, Ukraine can position itself as a hub for innovation in Eastern Europe. Successfully implementing state innovation policies aligned with European benchmarks will be critical. In summary, forming an innovative ecosystem is not just about fostering technological advancements but about creating a holistic environment where collaboration thrives, barriers are minimized, and creativity flourishes. The path toward establishing such an ecosystem requires concerted efforts from all stakeholders involved-government agencies must work together with educational institutions and businesses to cultivate a vibrant landscape for innovation. By doing so, Ukraine can pave the way for sustainable economic growth and regional development that benefits all citizens. Keywords: ecosystem, national security, region, economic recovery, sustainable development.

Simulation Model for a Sustainable Food Supply Chain in a Developing Country: A Case Study of the Banana Supply Chain in Malawi

Background: Sustainability in food supply chains is desired for production, logistics, and waste management. However, food supply chains (SCs) have complex systems that differ from other SCs. Managing such complexities is challenging for small and medium-sized enterprises (SMEs) due to their heightened constraints, such as limited resources and inadequate awareness. Despite the challenges, there is fragmented research, understanding, and approaches to assist SMEs. SC designs need to be adapted so that SMEs can navigate the challenges and avert high wastage. The main objective of this research was to identify the practices influencing sustainable SC designs in a developing country and create a simulation model to illustrate the potential benefits and challenges of promoting sustainability in the banana SC in Malawi. Methods: Mixed-methods research was utilised, employing a literature review, participant interviews, observations, and survey data collected from 353 participants from three districts in Malawi to gain insight into the banana SC problem, establish objectives, and develop a simulation model complemented by design science research. Results: The research identified awareness, collaboration, efficiency, governance, knowledge sharing, and resilience as sustainability practices in the case study banana SC, thus forming a sustainability model. Simulation results showed improvements in key performance indicators like shelf-life, lead-time, quality, throughput, and waste through SC operation reorganisation. Conclusions: Sustainable models must be tailored to the specific challenges inherent in developing food supply chains in developing nations. The development of the models has significant managerial implications, notably enhancing strategic planning, operational efficiency, risk management, alignment of sustainability goals, performance monitoring, stakeholder engagement, and resource optimisation.

Mixed fragmentation technique for securing structured data using multi-cloud environment (MFT-SSD)

Large data storage security is a topic of great interest to researchers, particularly in the age of big data where preserving data from theft, unauthorized access, and storage failure has become a crucial concern. To safeguard such data, encryption/decryption approaches have been employed, which are time-consuming and inefficient. The aim of this study is to develop a method, namely Mixed Fragmentation Technique for Securing Structured Data using Multi-Cloud Environment (MFT-SSD), for protecting large-scale data stored in a multi-cloud environment. This prevents insider attacks by adopting a mixed fragmentation approach to split the data into three files. For example, healthcare data is will be distributed among many clouds, each of which stores a partially unrecognized fraction of data without the need for an encryption or decryption layer. Comparing MFT-SSD to various encryption/decryption algorithms, our results show significant improvement; hence, the total performance of big data security is also improved.

‘Zero dollars and zero cents’: resourcefulness and DIY music scenes in rural and regional South Australia

ABSTRACT This article examines the role of ‘resourcefulness’ in small rural and regional music scenes. Drawing on case study fieldwork and ethnographic research in Port Lincoln, South Australia, I demonstrate the practices and tactics employed by participants in Port Lincoln’s local music scene. This research highlights the capacity of these scenes to ‘make do’ and adapt to change and disruption, while also emphasizing their agency. While rural and regional communities are often praised for their resilience, this rhetoric also effectively relieves policymakers of any urgency in addressing a lack of infrastructure and declining populations. As a result, the music industries in rural and regional Australia must adapt to the limitations of their existing infrastructure, capitalizing instead on strong community bonds to sidestep traditional, capitalist music industry structures. Music scenes in these regions are therefore characterized by a strong, grassroots but often fragmented approach to music scene production.

Prompt neutron emission calculations for the photon induced reactions of 238U and 232Th in the giant dipole resonance energy region

Prompt emission calculations for the photon induced fission of 238U and 232Th in the GDR energy region were performed by using (a) a systematic of input parameters for the most probable fragmentation approach (obtained on the basis of only spontaneous and neutron induced fission of actinides) and (b) photo-fission cross section ratios (expressing the fission probabilities at incident energies where multiple fission chances are involved) provided by nuclear reaction calculations performed with the EMPIRE code. The prompt neutron emission results describe well the experimental data recently measured at the laser Compton-scattering γ-ray source of the NewSUBARU facility, validating the use of this input parameter systematic for photon induced fission, too. The modelings and procedures described in this paper can constitute an useful tool for the prediction of prompt neutron emission data in photon induced reactions without any experimental information.

Facilitating new financial services in Latin America: an exploratory analysis of the sandbox model

This paper presents an end-to-end analysis of Fintech-driven sandboxes, with a particular focus on Latin America. Contrary to the procedural approach characterizing sandbox research, our analysis adopted the managerial perspective, and resulted in a theoretical and applicable framework. For this purpose, we initially characterized the motives, the conditions, and expected outcomes that sustain sandbox experimentation. Subsequently, based on the analysis of web resources, we drew comparisons between cases and the literature. Third, an inverted Gioia method is utilized to guide the characterization of sandbox models within our framework. The results indicate the presence of similar patterns in sandbox experimentation, providing justification for the choices made by each model to enable Fintech entrepreneurship. These patterns include the following: Latin America’s fragmented and gradual approach to engagement; the search for market efficiencies around payments, investment, and lending; the primacy of capital flow intermediation, and the participation of both incumbent and new ventures. These findings contribute to the advancement of our knowledge about the influence of sandbox model designs on Fintech development, with practical implications for entrepreneurs and regulators. Furthermore, the framework proposed in this paper may prove beneficial in understanding institutional experimentation and advancing Fintech in other world regions beyond Latin America.

(Innovative) Teaching Models in Doctoral Dissertations at the Faculty of Teacher Education in the Republic of Serbia

Starting from conceptual definitions and theoretical framework of the research problem, this paper offers the findings of empirical non-experimental research on the global and fragmented approach: a) assessment of the representation of (innovative) models of class teaching within the university repository of defended doctoral dissertations in the Republic of Serbia, and b) assessment of the innovative characteristics of selected doctoral dissertations through the analysis of reports from professional committees evaluating completed doctoral dissertations. All 109 PhD dissertations defended at Serbia’s six state faculties of teacher education between 2005 and 2022 are included in the overall sample. The second component of the research specifies a fragmented or deliberate/ stratified sample that consists of 36 defended PhD dissertations and 36 evaluation reports for the approval of PhD dissertations that inherently include novel teaching models. Both quantitative and qualitative content analysis were used in the research. The units of analysis include: the title of the defended doctoral dissertation, keywords presented in the doctoral thesis (quantitative analysis), and the content of the Evaluation Report of the doctoral dissertation, specifically the segment on scientific contribution (qualitative analysis). Based on the analysis of dissertation titles and keywords, it was found that one-third of doctoral dissertations focus on research in the field of (innovative) teaching models. The analysis revealed that not all models are equally represented in different subject methodologies of class teaching, nor within individual methodologies. Content analysis showed that the selected theses are dedicated to the specified problem, they support the issue of the innovation of the teaching process and indicate that there is room for innovation in the teaching process in a creative sense. Future PhD dissertations with a thematic focus on innovative teaching models should be directed towards the affirmation of research on different work models (of the entire corpus) in subject methodologies, the synthesis of innovative teaching models within the same methodology of class teaching, exploration of learning material most suitable for implementing a specific innovative work model within various methodologies of class teaching...

A Novel Fragmented Approach for Securing Medical Health Records in Multimodal Medical Images

A Novel Fragmented Approach for Securing Medical Health Records in Multimodal Medical Images

Determinants of Tax Evasion in the Perspective of Enforcement, Psychology, Trust, and Services

ABSTRACT Indonesia’s scant tax ratio underscores a grave issue of tax evasion. Past studies have scrutinized tax evasion through distinct lenses, yet this fragmented approach is beset with limitations, hampering its efficacy in preempting tax evasion conduct. This study endeavors to amalgamate these perspectives to scrutinize the determinants of tax evasion comprehensively. Employing a quantitative methodology, a questionnaire was disseminated between April and August 2023, yielding 236 valid responses from individual taxpayers. Data analysis encompassed descriptive analysis, classical assumption testing, multiple linear regression analysis, Moderated Regression Analysis (MRA), and hypothesis testing. Findings reveal an interplay among the four dimensions of tax evasion in curbing its incidence. Nevertheless, the moderating influence of demographics on this relationship proved nonsignificant. Conversely, the integration of Information and Communication Technology (ICT) bolsters the efficacy of the service perspective in mitigating tax evasion. Thus, a nuanced understanding of tax evasion necessitates a multifaceted approach. Furthermore, ICT adoption emerges as a potent instrument in fortifying the impact of the service perspective on curtailing tax evasion, urging governmental and tax regulatory bodies to consider leveraging ICT to bolster tax compliance.

P-791 The 4-step ASCOT technique modifies the plasma proteome of patients with premature ovarian insufficiency to resemble that of normoresponder women

Abstract Study question Does the 4-step ASCOT technique induce any modification in the plasma proteomic profile of women with premature ovarian insufficiency (POI)? Summary answer The 4-step ASCOT technique elicits substantial changes in plasma proteome of POI patients, leading to a more similar composition to that observed in normoresponder women. What is known already Nowadays, treatment of POI women is still challenging and different approaches have been proposed to improve their reproductive potential, including stem cells (SC) and platelet-rich plasma (PRP). Previous studies showed that plasma treatments containing a combination of both SC-secreted and platelet-enclosed factors are the most promising regenerative treatment in mouse and human ovarian tissues. Therefore, we designed the 4-step ASCOT technique, consisting in: 1) SC mobilization from bone marrow to peripheral blood to enrich plasma with SC secreted factors, 2) Platelet concentration, 3) platelet activation to deliver additional growth factors and 4) direct ovarian injection. Study design, size, duration Experimental study with plasma samples of 10 POI women undergoing the 4-step ASCOT reactivation technique (NCT04475744) and 5 normoresponder fertile women. Peripheral plasma samples from POI women were collected at recruitment (Pre) and three months after SC-PRP ovarian injection (Post). Proteomic profiles of Pre and Post plasma samples were compared between them and with the normoresponder samples. Participants/materials, setting, methods Proteins were isolated from all plasmas and quantified by the data-independent acquisition parallel accumulation serial fragmentation (diaPASEF) approach. Principal component analysis was performed to compare the plasma proteomic profile of pre- and post-treatment samples, applying the vip function to determine which proteins explained the differences between both time-points (vip>1.5). Then, ElasticNet and Limma methods were employed to determine the statistically significant changes and functional analysis performed to establish the enriched KEGG pathways (false discovery rate<0.05). Main results and the role of chance Plasma proteome of POI patients at recruitment exhibited a distinctly different proteomic pattern from that of normoresponder women. However, principal component analysis revealed a clear distinction between plasma samples obtained from POI patients at recruitment and three months after the 4-step ASCOT technique (principal component 1 (PC1) accounting for 20% of explained variance, and PC2 for a 15.4%). Differences between both time-points were mainly determined by 56 proteins involved in processes with a key role in ovarian function, such as complement and coagulation cascades, platelet activation, Hippo pathway, cell cycle, oocyte meiosis and PI3K-Akt signaling pathway. Of these, fifteen proteins exhibited significantly differential expression between both time points, including the age-related APOF protein and the GC vitamin D binding protein, which was the most prominently changed protein after the treatment. Interestingly, the 4-step ASCOT technique allowed the plasma proteome of patients with POI to reach a similar profile to that observed in normoresponder women. In fact, a detailed analysis uncovered 72 differentially expressed proteins between pre-treatment and normoresponder samples, with only 28 in post-treatment vs. normoresponders. Moreover, the magnitude of expression changes, when compared to normoresponder women, was more significant in pre-treatment than in post-treatment samples. Limitations, reasons for caution Further research will be necessary to determine the extent of the regenerative effects of the identified differentially expressed proteins within the ovaries, and their direct implications in ovarian aging and function. Wider implications of the findings Our findings highlight that the 4-step ASCOT technique induces changes in plasma proteome of POI patients, reaching similar values to normoresponders. These changes, that remain for few months, might contribute to ovarian reactivation, being the stepping stone to design more effective and personalized treatments for patients with impaired ovarian reserves. Trial registration number NCT04475744