Objectives: The aim of this in-vitro study is to assess and compare the load capacity of teeth with canals prepared using progressive taper versus regressive taper. Methods: Twenty-seven extracted mandibular molars were categorized into three equal groups. The negative control group involved teeth that were accessed and restored with composite resin without canal preparation. The regressive canal preparation group used the TruNatomy file system, while the progressive canal preparation group employed the ProTaper Gold system. Subsequently, all samples underwent loading using a universal testing machine until tooth fracture occurred. The force required to fracture each tooth was recorded in Newton. Results: The highest value was found in samples without preparation 1009.68±57.28 N, followed by TN group 979.04±31.16 N, while the lowest value was found in PTG 966.47±17.51N. There was no significant difference between different groups (p=0.118). Conclusions: The root canal taper did not exert a significant influence on the longevity or fracture resistance of the tooth.