This study investigates the low-cycle fatigue (LCF) behavior of two types of dissimilar steel welded joints (DSWJs): AISI 1035 & S550Q and AISI 1020 & S550Q. Digital image correlation (DIC) and finite element analysis (FEA) were used to analyze the heterogeneous strain distribution. The results reveal that the location of maximum strain, which is load-dependent and related to strength mismatch, corresponds to the fracture location of the LCF specimens. Specifically, the crack initiation site shifts from the weld toe to the base metal as strain increases. Moreover, the weld reinforcement of the DSWJs significantly affects the failure site transition, introducing strain concentration at lower stress levels while enhancing deformation resistance at higher stress levels, as evidenced by DIC strain measurements and FEA. These findings highlight the importance of joint reinforcement profile, strength mismatch and external load level in designing DSWJs for wind turbine towers against LCF failure.