The stress threshold of deep reservoir shale subjected to fracturing fluid immersion is an important factor affecting fracture initiation and propagation during fracturing. However, little information has been reported on the effect on shale of soaking at high temperature and high pressure (HTHP). In this study, immersion tests and triaxial compression tests were carried out at reservoir temperature and in-situ stress on the downhole cores with different mineral compositions. The characteristics of stress thresholds, i.e., crack initiation stress (σci), crack damage stress (σcd), and peak deviator stress (σp), of shale affected by the different times of soaking with low-viscosity fracturing fluid (a) and the different viscosity fracturing fluids (a, b, and c) were investigated. The results show that hydration at HTHP has a significant softening effect on the stress thresholds (σci, σcd, σp) of reservoir shale, but the softening rate varies for samples with different mineral compositions. The crack initiation stresses of quartz-rich and clay-rich shales treated with different soaking times and different soaking media remain almost unchanged in the range of 47 to 54% of the corresponding peak strength, while the crack initiation stresses of carbonate-rich shales are significantly affected. The ratio σcd/σp of quartz-rich shale is significantly affected by the different viscosity fracturing fluids (a, b) and the different times of soaking with low-viscosity fracturing fluid (a), while clay- and carbonate-rich shales are less affected. The results of this study can provide a reference for the fracturing design of deep shale gas development.