Fractionation Of Aqueous Extract Research Articles

Proteins in Fish Muscle: 17. Fractionation of Aqueous Extracts with Zinc Acetate

Zinc acetate in low concentrations has been used to fractionate aqueous extracts of cod and haddock muscle. Approximately 18% of the cod proteins extracted remained soluble after addition of 0.1M zinc acetate and subsequent treatment with KH2PO4 buffer. This fraction consisted largely of two components of the original cod extract, and the same fraction from haddock was very similar. Fractionation on DEAE-cellulose removed the minor components, but failed to separate the main ones. The protein of the cod fraction contained an unusually large proportion of phenylalanine.