Propolis exhibits a high content of flavonoids and phenolic acids, hence demonstrating significant antioxidant properties, which have received considerable attention as antimicrobial agents. This study aimed to assess the total phenolic and flavonoid contents of Turkish brown propolis from Gaziantep and Adana provinces, as well as their in-vitro antioxidant, antibacterial, and antifungal activities alone and in combination with Arthrospira platensis. The purification and extraction of propolis were done using 80% ethyl alcohol. The results showed that there was no significant difference in total flavonoid content and antimicrobial activity between the two different propolis samples, but significant differences in their antioxidant activity and total phenolic content were observed (p < 0.05). Both propolis and A. platensis showed good antimicrobial activity against the tested microorganisms alone and in combination. The fractional inhibitory concentration index values demonstrated a synergistic effect between propolis and A. platensis against Staphylococcus aureus, Escherichia coli, and Candida albicans. The results of this study provide empirical evidence that endorses the conventional use of propolis and A. platensis as agents with antibacterial properties. Furthermore, these findings propose that the combination of propolis and A. platensis has promise as a prospective approach for the development of natural antibiotics in forthcoming research endeavors.