Low Molecular Weight Fraction Research Articles

Phytoplasma DNA Enrichment from Sugarcane White Leaves for Shotgun Sequencing Improvement

Sugarcane white leaf (SCWL) disease, caused by Candidatus Phytoplasma sacchari, poses a significant threat to sugarcane cultivation. An obligate parasite, phytoplasma is difficult to culture in laboratory conditions, making the isolation of its DNA from the massive amount of plant host DNA extremely challenging. Yet, the appropriate amount and quality of plant microbiome-derived DNA are key for high-quality DNA sequencing data. Here, a simple, cost-effective, alternative method for DNA isolation was applied using a guanidine-HCl-hydroxylated silica (GuHCl-Silica)-based method and microbiome DNA enrichment based on size-selective low-molecular-weight (LMW) DNA by PEG/NaCl precipitation. qPCR analysis revealed a significant enrichment of phytoplasma DNA in the LMW fraction. Additionally, the NEBNext Microbiome DNA enrichment kit was utilized to further enrich microbial DNA, demonstrating a remarkable increase in the relative abundance of phytoplasma DNA to host DNA. Shotgun sequencing of the isolated DNA gave high-quality data on the metagenome assembly genome (MAG) of Ca. Phytoplasma sacchari SCWL with completeness at 95.85 and 215× coverage. The results indicate that this combined approach of PEG/NaCl size selection and microbiome enrichment is effective for obtaining high-quality genomic data from phytoplasma, surpassing previous methods in efficiency and resource utilization. This low-cost method not only enhances the recovery of microbiome DNA from plant hosts but also provides a robust framework for studying plant pathogens in complex plant models.

Impact of molecular architecture and draw ratio on enhancement of targeted mechanical properties of machine direction oriented polyethylene films produced after blown film extrusion

Conventional multi-material multi-layer flexible packaging offers excellent properties. However, it has recycling challenges, necessitating a shift to mono-material multi-layer flexible packaging for example all-polyethylene (PE) packaging which can be tailored through various synthesis and processing methods for different layers. In this work, we study how the key molecular properties (number-average molecular weight ( Mn), weight-average molecular weight ( M w), molecular weight distribution (MWD), comonomer content (short chain branching)) and machine direction orientation (MDO) process draw ratio (MDX) influence the final morphology and mechanical properties of MDO-PE films which are intended as the outer layer of mono-material all-polyethylene multi-layer flexible packaging. Five PE grades and various blends were extruded and blown into films. Selected blown films were machine direction oriented to obtain the final MDO-PE films. Furthermore, one selected PE blown film was processed at different MDO process draw ratios while keeping other process parameters constant. From the molecular properties point of view, the higher molecular weight fractions provide a higher possibility for uniform stretching whereas lower molecular weight fractions provide a higher natural draw ratio and therefore higher modulus and stiffness enhancement. Further, the results show that increasing MDO process draw ratio leads to more fibrillation and increased crystallinity. Consequently, the tensile modulus and stiffness at the higher draw ratios increase as well and are comparable to conventionally used polymers in outer layers of multilayer flexible packaging. Thus, this work demonstrates that MDO-PE films with enhanced modulus can provide sufficient stiffness for the design of outer layer of mono-material multi-layer all-PE packaging which presents higher potential for mechanical recyclability.

Scallop farming impacts on dissolved organic matter cycling in coastal waters: Regulation of the low molecular weight fraction

To elucidate the impacts of scallop farming on the biogeochemical characteristics of low molecular weight (LMW, <1 kDa) dissolved organic matter (DOM), samples collected from a bay scallop mariculture area (MA) and its surrounding areas were determined for absorption and fluorescence spectroscopy after microfiltration and centrifugal ultrafiltration. The values of absorption coefficient a350 showed a spatial variation trend of inshore area (IA) > MA > non-mariculture area (NMA) for both bulk (<0.7 μm) and LMW fractions. Four fluorescent components, namely two protein-like components (tryptophan-like C1 and tyrosine-like C2) and two humic-like components (microbial humic-like C3 and terrestrial humic-like C4), were identified. Scallop farming influenced DOM transformation by altering phytoplankton abundance and promoting microbial degradation. In July, the net contributions of phytoplankton to the spectroscopy parameters of LMW-DOM in the surface seawater were 11.0% for a350, 4.3% for C1, 0.8% for C2, 0.6% for C3 and 3.0% for C4, respectively; the corresponding values of bulk DOM in the surface seawater were 24.3% for a350, 20.1% for C1, 5.9% for C2, 2.0% for C3, 2.9% for C4, respectively. Compared with NMA, the contributions of microbial degradation to a350 in MA's surface seawater increased by 9.0% for LMW-DOM and 6.9% for bulk DOM in July; however, the effects on different fluorescent components varied. In August, compared with NMA, the contributions of microbial degradation to spectroscopy parameters in the bottom water of MA decreased by 35.7% for a350, 6.3% for C2, 1.3% for C3, and 4.4% for C4 for LMW-DOM fraction; for bulk DOM, the corresponding contribution decreased by 10.8% for C1. These variations indicate that protein-like substances from scallop aquaculture are easily degraded into LMW substances, while humic-like substances degradation diminishes over time.

In-depth analysis of natural organic matter fractions in drinking water treatment performance: Fate and role of humic substances in trihalomethanes formation potential

In this study we investigate the compositional changes in dissolved organic matter (DOM) fractions across diverse water sources and treatment processes in three Drinking Water Treatment Plants (DWTPs). High-Performance Size Exclusion Chromatography coupled with Diode Array Detection and Organic Carbon Detection (HPSEC-DAD-OCD) was employed to characterize DOM fractions, offering insights into treatment optimization. We examine bulk water parameters, DOM distributions, and the efficiency of treatment trains in reducing DOM fractions. Results reveal distinct DOM composition profiles in river-sourced versus reservoir-sourced waters, with implications for treatment processes. Coagulation, Granular Activated Carbon (GAC) adsorption, Electrodialysis Reversal (EDR), and Ion Exchange (IEX) were evaluated for their efficacy in removing DOM fractions. The analysis highlights the effectiveness of coagulation in reducing high molecular weight (MW) fractions, while GAC filtration targets lower MW fractions. EDR shows significant removal of anions and aromatics, while IEX demonstrates high removal efficiencies for removing humic substances (HS) fractions. Spectroscopic analysis further elucidates changes HS sub-fractions and their role in disinfection by-products (DBP) formation. To quantitatively assess the relationship between HS sub-fractions and trihalomethane formation potentials (THMFP), Pearson correlation analysis were conducted, unveiling robust associations between HS sub-fractions and THM-FP that can be predicted by surrogate parameters such as A254.

Antimicrobial, Antioxidant and Anti-Inflammatory Activities of the Mucus of the Tropical Sea Slug Elysia crispata.

Elysia crispata (Sacoglossa, Gastropoda) is a tropical sea slug known for its ability to incorporate functional chloroplasts from a variety of green macroalgae, a phenomenon termed kleptoplasty. This sea slug, amenable to laboratory cultivation, produces mucus, a viscous secretion that serves diverse purposes including protection, locomotion, and reproduction. In this study, we profiled the antimicrobial, antioxidant, and anti-inflammatory activities of the mucus of this sea slug. Results revealed inhibitory activity against several bacterial strains, more pronounced for Gram-negative bacteria. Particularly interesting was the strong inhibitory effect against Pseudomonas aeruginosa, a bacterial species classified by the WHO as a high-priority pathogen and associated with high-risk infections due to its frequent multidrug-resistant profile. Similar inhibitory effects were observed for the mucus native protein extracts, indicating that proteins present in the mucus contributed significantly to the antimicrobial activity. The mucus also showed both antioxidant and anti-inflammatory activities. The latter activities were associated with the low molecular weight (<10 kDa) fraction of the mucus rather than the native protein extracts. This study opens the way to further research on the biotechnological applications of the mucus secreted by this unique marine organism, particularly as an antimicrobial agent.

Effect of low molecular weight phenols on the in-mouth sensory perception of high molecular weight phenols by analyzing reconstituted wines

This research aimed to assess the contribution of monomeric phenols to the perception of bitterness and astringency by means of wine reconstitution experiments. Six wines with distinct total polyphenol indexes were selected and fractioned by preparative liquid chromatography into two fractions. Thirty-one reconstituted wines were prepared by combining distinct low molecular weight fractions (L) with different high molecular weight fractions (H). In-mouth attributes of reconstituted and reference wines were described by a trained sensory panel. Fractions were chemically characterized by UPLC-MS. No significant differences among sensory results were found between reference and reconstituted wines. Bitterness and astringency perception were strongly influenced by total proanthocyanidins content. Flavan-3-ol monomers and dimers were only responsible for astringency in wines with a low content of proanthocyanidins, as was the case in rosé wine. Quercetin-3-O-glucuronide and several anthocyanins presented correlations with bitterness in the reconstituted wines; notwithstanding, several interactions with this attribute were observed.

Development of sustainable Lignin-Based coatings for layered double Hydroxides: Enhancing synergistic Anti-Aging properties in bitumen

Layered Double Hydroxides (LDHs) have poor dispersibility in bitumen and limited effectiveness in enhancing its thermal oxidative aging resistance. To address these issues, this study aims to purify lignin to improve its radical scavenging efficiency and use the purified lignin to coat LDHs (Φ@LDHs), thereby increasing their lipophilicity and radical scavenging efficiency for use in bitumen. sodium lignosulfonate (SL) was fractionated into five parts using a stepwise water/ethanol fractionation method, and these fractions were then applied to coat the LDHs to produce Φ@LDHs. The morphology, antioxidant activity, and chemical structure of Φ@LDHs were analyzed. Subsequently, the effect of Φ@LDHs on the aging properties of bitumen was evaluated. The test results indicated that SL and its fractions effectively coated the surfaces of LDHs, thereby enhancing their interlayer spacing and hydrophobicity. Among all fractions, lower molecular weight fractions, F4 and F5, retained most of the phenolic hydroxyl groups in SL, demonstrating significant antioxidant activities. The incorporation of F4@LDHs and F5@LDHs effectively mitigated changes in rheological properties of bitumen and oxidation product formation during thermal oxidation and UV aging, with F5@LDHs showing the best performance. Furthermore, a synergistic anti-aging mechanism was elucidated for the F4@LDHs and F5@LDHs composites. The purified F4 and F5 fractions not only improved the compatibility of LDHs with bitumen but also delayed the oxidative reactions initiated by free radicals to achieve a synergistic anti-aging effect.

Periodic membrane fractionation of freshwater organic matter reveals various reactivity patterns during chlorine/chloramine disinfection

Although drinking water disinfection has significantly reduced mortality from waterborne diseases, the formation of potentially harmful disinfection by-products (DBPs) remains a concern today. These DBPs emerge from the reaction of dissolved organic matter (DOM) with chlor(am)ine during disinfection. Identifying these DBP precursors from heterogeneous and complex DOM mixtures is challenging and to address this, a novel membrane fractionation protocol was developed to isolate and identify the organic DBP precursors from fresh water sources. A tight ultrafiltration and nanofiltration membrane were carefully selected to partition DOM into three molecular weight (MW) fractions: high (>20 kDa), medium (0.3–20 kDa) and low (<0.3 kDa) as defined by high performance-size exclusion chromatography- total organic carbon analysis. A mathematical tool was developed to optimize the fractionation protocol. Therefore, ultrafiltration was executed before nanofiltration and the concentration factor was maximized without inducing fouling to obtain the purest fractions. The mathematical tool was also set in place to predict the necessary diafiltration factor for each individual fractionation experiment based on the initial organic matter composition of the surface water allowing the fractionation protocol to be effective at all times. The protocol was applied to surface water samples collected six times across three seasons yielding a fraction enriched in high MW compounds (up to 50 %), a fraction having more than 80 % of medium MW compounds and a fraction only containing low MW compounds. Although the medium MW fraction showed the highest reactivity for the majority of the investigated DBPs, the low MW fraction showed high reactivity for iodinated trihalomethanes during chlorination and for haloacetonitriles during chloramination. The high MW fraction had the lowest reactivity towards DBPs, which can have important implications for a drinking water treatment since this fraction is generally the most effectively removed by e.g. coagulation, while the more important fractions for DBP formation such as the medium and low MW compounds remain in the water until the disinfection step.

Effect of Fly Ash in Pyrolysis of HDPE, LDPE and PP Plastic Waste

Fly ash is generally obtained as a by-product from the combustion of coal and other waste materials. It is used for making bricks, but it has few limitations. The fly ash consists of Silica, Alumina, and other metal oxide components in minor quantities. Fly ash particles are observed in the range of nanometers to micrometers and can act as a catalyst in various reactions. The use of low-cost catalysts in the pyrolysis of thermoplastic waste would achieve a high percentage of low molecular weight fractions in liquid form which increases its applicability in commercial sectors. Hence, there is a need to enhance these fractions to achieve a sustainable approach in the catalytic pyrolysis process. fly ash, being a side product, is very cheap, so its effect on the plastic waste pyrolysis process has been studied. In the present research paper, Physical &amp; chemical characterization of fly ash has been carried out. As fly ash consists of different metal oxides in proportion, its applicability in the process of pyrolysis of HDPE, LDPE, and PP waste has been studied. The different weight percent of fly ash (i.e., 5, 10, 15, 20) have been tried in all pyrolysis experiments. It has been observed that 5 wt % fly ash is effective for enhancing the yield of liquid fuel as compared to that without a catalyst. Liquid fuel obtained from catalytic pyrolysis of HDPE, LDPE, and PP waste with Fly ash consists of a high percent of low molecular weight fractions as compared to that of liquid fuel without catalyst, which has been concluded by calorific values &amp; GC-MS result.

Kinetic insights into heavy crude oil upgrading in supercritical water

Heavy crude oil upgrading in a supercritical water environment is a promising technology owing to diverse advantages such as heteroatom removal, high yields of low molecular weight fractions, and inhibition of coke production. In order to increase the performance of this process, some challenges need to be overcome by researching the reaction mechanisms and developing proper numerical models. Therefore, the kinetic studies reported in the literature for hydrothermal upgrading of heavy crude oil under supercritical water conditions and their results were systematically analyzed and discussed to achieve a better understanding of the diverse approaches considered to determine the distribution of reaction products and limitations. Additionally, fundamental aspects of the kinetic models, including experimental considerations and numerical methodologies applied for kinetic parameter estimation, are reviewed to obtain representative information about the reactant system that can be subsequently integrated into reactor design models or reservoir simulations.

Nanofiltration membranes in asymmetric flow field-flow fractionation for improved organic matter size fractionation

Size fractionation of organic matter (OM) by asymmetric flow field-flow fractionation (FFFF) with ultrafiltration (UF) is limited by solute loss and peak resolution, particularly for low molecular weight fractions (<10 kDa). Nanofiltration (NF) with a molecular weight cut-off (MWCO) of ≤1 kDa can achieve significant OM retention and may improve OM fractionation. NF membranes with MWCOs ranging from 0.3 to 3 kDa were used to evaluate the retention and fractionation of nine OMs in the humic, polyphenol, biopolymer, and low molecular weight organic classes in stirred cell NF and FFFF. Membranes with an MWCO of 0.2–0.3 kDa (with a pure water permeability of 5–16 L.m−2.h−1.bar−1) with low OM interaction exhibited high peak recovery and resolution and hence improved OM fractionation. Loose NF (MWCO 3 kDa) exhibited a poor peak recovery because of a high OM loss due to both adhesion and permeation, particularly at high ionic strengths (>10 mM). A mixture of nine types of OM and natural organic matter samples from rivers, swamps and lakes achieved good fractionation with a 0.3 kDa (NF270) membrane at low ionic strength. The performance of NF for the analysis of OM and colloids in the smallest size range (≤1 kDa MW) could be further improved by optimizing the salt composition of the mobile phase and by overcoming the pressure limitations of FFFF channels.

Ultrasound Depolymerization and Characterization of Poly- and Oligosaccharides from the Red Alga Solieria chordalis (C. Agardh) J. Agardh 1842.

Red seaweed carrageenans are frequently used in industry for its texturizing properties and have demonstrated antiviral activities that can be used in human medicine. However, their high viscosity, high molecular weight, and low skin penetration limit their use. Low-weight carrageenans have a reduced viscosity and molecular weight, enhancing their biological properties. In this study, ι-carrageenan from Solieria chordalis, extracted using hot water and dialyzed, was depolymerized using hydrogen peroxide and ultrasound. Ultrasonic depolymerization yielded fractions of average molecular weight (50 kDa) that were rich in sulfate groups (16% and 33%) compared to those from the hydrogen peroxide treatment (7 kDa, 6% and 9%). The potential bioactivity of the polysaccharides and low-molecular-weight (LMW) fractions were assessed using WST-1 and LDH assays for human fibroblast viability, proliferation, and cytotoxicity. The depolymerized fractions did not affect cell proliferation and were not cytotoxic. This research highlights the diversity in the biochemical composition and lack of cytotoxicity of Solieria chordalis polysaccharides and LMW fractions produced by a green (ultrasound) depolymerization method.

Effect of Crude Extract from the Sea Anemone Bunodeopsis globulifera on Voltage-Gated Ion Channels from Central and Peripheral Murine Nervous Systems.

Sea anemones are an important source of bioactive compounds with potential pharmacological applications. Their toxins are produced and stored in organelles called nematocysts and act on specific targets, including voltage-gated ion channels. To date, sea anemone toxins have demonstrated effects on voltage-gated sodium and potassium channels, facilitating investigations into the structure and function of these proteins. In this study, we evaluated the effect of Bunodeopsis globulifera sea anemone crude extract, and of a low molecular weight fraction, on voltage-gated sodium and calcium channels within the murine nervous system. Notably, the crude extract led to a significant reduction in total sodium current, while also triggering calcium-dependent glutamate release. Furthermore, the low molecular weight fraction, in particular, enhanced total calcium currents and current density. These findings underscore the existence of sea anemone toxins with diverse mechanisms of action beyond those previously documented.

Application of high-pressure homogenization-assisted pH-shift to enhance techno-functional and interfacial properties of lentil protein isolate

High-pressure homogenization (HPH) is a promising physical non-thermal approach to improve protein techno-functionality. This study aims to examine the effects of HPH on the lentil proteins through the perspective of the interfacial adsorption mechanism. The impact of HPH treatment on lentil protein isolate (LPI) at varying pressure levels (0–150 MPa) was determined using several analytical techniques, including SDS-PAGE, FTIR, solubility, and techno-functional properties (foaming and emulsifying properties), alongside analyses of interfacial tension and interfacial shear rheology at the o/w and a/w interfaces for two pH values (2.0 and 4.5). Results reveal that HPH treatment up to 100 MPa effectively unfolds lentil proteins by disrupting disulfide-bonded subunits into lower molecular weight fractions and unfolding highly-ordered secondary structures into random coils. LPI's capacity to produce emulsions and foams was found to be enhanced concurrently with these physicochemical changes, particularly at pressures up to 50 MPa. The findings aligned with the interfacial tension and shear rheology analyses, which show that proteins can form interfacial viscoelastic films on both o/w and a/w interfaces. Furthermore, the interfacial behavior of LPI and the effect of HPH on the interfacial behavior were found to be pH-dependent. The lower interfacial tension and the higher interfacial viscoelastic moduli (G′ and G″) were recorded at 50 MPa and 0 MPa at pH 2.0 and 4.5, respectively. These results stated that the effects of the HPH on the technofunctionality of LPI can be further enlightened by investigating the interfacial adsorption kinetics.

Comparative analysis of rice bran and sesame protein hydrolysates in inhibiting foodborne pathogens: efficacy against Escherichia coliO157:H7 and Listeria monocytogenes

SummaryThis study explores the antibacterial potential of hydrolysates derived from rice bran protein (RBP) and sesame protein (SP) against Escherichia coli O157:H7 and Listeria monocytogenes. RBP and SP were hydrolysed by protease from Bacillus licheniformis (labelled as RBPH‐B and SPH‐B) and α‐chymotrypsin (denoted as RBPH‐C and SPH‐C), and subsequently fractionated into four molecular weight (MW) categories: F1 (&gt;100 kDa), F2 (10–100 kDa), F3 (1–10 kDa), and F4 (&lt;1 kDa). The antibacterial activities of these hydrolysates and their fractions were systematically evaluated. The results indicated that RBPH‐B and RBPH‐C, along with their fractions, exhibited notable efficacy against L. monocytogenes. Conversely, both SPH‐B, SPH‐C, and their fractions showed pronounced antibacterial activities against both pathogens. Notably, lower MW fractions (SPH‐B, SPH‐B‐F2, and SPH‐B‐F3) demonstrated superior antibacterial properties, effectively reducing bacterial populations to below detection limits (≥6 log CFU mL−1) at a concentration of 100 mg mL−1. Further analysis revealed that SPH‐B‐F3 markedly reduced the reaction time required to inhibit or eliminate E. coli O157:H7 and L. monocytogenes, with comparable times of 6 and 4 h, respectively. These findings highlight the potential application of specific SPH‐B fractions as antibacterial agents in the food industry.

Study of humic-like substances of dissolved organic matter using size exclusion chromatography and chemometrics

The study of dissolved organic matter (DOM) presents a significant challenge for environmental analyses and the monitoring of wastewater treatment plants (WWTPs). This is particularly true for the tracking of recalcitrant to biodegradation dissolved organic matter (rDOM) compounds, which is generated during the thermal pretreatment of sludge. This study aims to develop analytical and chemometric methods to differentiate melanoidins from humic acids (HAs), two components of rDOM that require monitoring at various stages of wastewater treatment processes due to their distinct biological effects. The developed method implements the separation of macromolecules through ultra-high-performance liquid chromatography size-exclusion chromatography (U-HPLC SEC) followed by online UV and fluorescence detection. UV detection was performed at 210, 254, and 280 nm, and fluorescence detection at six excitation/emission pairs: 230/355 nm, 270/355 nm, 240/440 nm, 270/500 nm, 330/425 nm, and 390/500 nm. Chromatograms obtained for each sample from these nine detection modes were integrated and separated into four molecular fractions: >40 kDa, 20–40 kDa, 10–20 kDa, and <10 kDa. To enhance analytical resolution and normalize the data, ratios were calculated from the areas of chromatographic peaks obtained for each detection mode. The results demonstrate the utility of these ratios in discriminating samples composed of HAs, melanoidins, and their mixtures, through principal component analysis (PCA). Low molecular weight fractions were found to be specific to melanoidins, while high molecular weight fractions were characteristic of HAs. For the detection modes specific to melanoidins, UV absorbance at 210, 254, and 280 nm were predominantly present in the numerators, with tryptophan-like fluorescence emissions in the denominators. Conversely, fluorescence emissions largely represented both numerators and denominators for HAs. This online method also enables the discrimination of pseudo-melanoidins, compounds revealing a nitrogen deficiency in their chemical structures.

Impact of biochemical properties on the gelation of EPS extracted from aerobic granules

Extracellular Polymeric Substances (EPS) extracted from aerobic granular sludge are a promising source of gel-forming biopolymers. Gaining insights on the charge distribution of these polymers was performed through a fractionation using anionic chromatography. A gelling factor (GF) based on the apparent gel volume and the reactivity of polymers to calcium was attributed to each eluted fraction. The GF values of the eluted anionic EPS correlated with their negative charge. A GF of 0.04 ± 0.03 is measured for molecules eluted at NaCl 0.25 M and a GF of 0.32 ± 0.02 for the molecules eluted at NaCl 0.75 M. However, upon reaching a very high negative charge (elution at NaCl 1 M), the gelation capacity decreases. The size distribution of gelling molecules was analyzed by Size Exclusion Chromatography. High Molecular Weight (MW) molecules are predominantly involved in gelation (between 65 % and 100 % of the high molecular weight fraction from each sample) while low MW molecules participation is relatively limited (lower than 40 % of the low molecular weight fraction from each sample). Moreover, infra-red spectroscopy analysis of the molecules before and after calcium addition showed that mostly proteins and polysaccharides contribute to the hydrogel formation. Colorimetric methods showed that around 60 % of the uronic acids present in the samples could form hydrogels whereas proteins’ participation varied between 0 % and 100 % depending on the fraction.Thus, modulating EPS extraction and purification methods to ensure the preservation of their molecular weight and the selection of appropriate negative charge could be a strategy for EPS valorization.

Molecular Weight Distribution of Humic Acids Isolated from Calcic Cryosol in Central Yakutia, Russia.

The transition of soils into fallow state has a significant impact on the accumulation and transformation of soil organic matter (SOM). However, the issue of SOM transformation as a result of soil transition to fallow state in cryolithozone conditions is insufficiently studied. The aim of this study is to investigate the molecular weight (MW) distribution of humic acids (HAs) isolated from soils of central Yakutia. Native, fallow and agricultural soils in the vicinity of Yakutsk city were studied. MW distributions of HA preparations were obtained on an AKTAbasic 10 UPS chromatographic system (Amersam Biosciences, Uppsala, Sweden) using a SuperdexTM 200 10/300 GL column (with cross-linked dextran gel, fractionation range for globular proteins 10-600 kDa). The data on the molecular-mass distribution of HAs of fallow and agricultural soils of Central Yakutia were obtained for the first time. According to the obtained data, it was found that the highest carbon content in the structure of HAs was observed in agricultural soils (52.56%), and is associated with soil cultivation and fertilizer application. Among the HAs of fallow soils, we note that those soils that are in the process of self-vegetation have a relatively high carbon content in the HAs (45.84%), but the highest content was observed in fallow soils used as hayfields (49.98%), indicating that the reinvolvement of agriculture in fallow soils leads to an increase in the carbon content of HAs. According to the data of the MW distribution of HAs, it was found that the highest content of a high MW fraction of HAs was recorded in native soil (18.8%); this is due to the early stages of humification and the low maturity of organic matter. The highest content of a low MW fraction of HAs was recorded in agricultural soil (73.3%); this is due to the formation of molecular complexes of a "secondary" nature, which are more stable in the environment than the primary transformation products of humification precursors. The molecular composition of the HAs of fallow soils in the process of self-overgrowing is characterized by values closer to the HAs of native soils, which indicates their transformation towards HAs of native soils. The obtained results indicate that the reinvolvement of fallow soils leads to the transformation of the molecular composition of HAs towards HAs of agricultural soils, and to an increase in the resistance of SOM to biodegradation.

Molecular weight-dependent differences in spectral properties and metal-binding behaviors of dissolved organic matter from different lakes

Dissolved organic matter (DOM) plays an important role in governing metal speciation and migration in aquatic systems. In this study, various DOM samples were collected from Lakes Erhai, Kokonor, and Chaka, and size-fractionated into high molecular weight (HMW, 1 kDa–0.7 μm) and low molecular weight (LMW, <1 kDa) fractions for measurements of dissolved organic carbon (DOC), spectral properties, and metal binding behaviors. Our results demonstrated that samples from Lake Chaka exhibited the highest DOC concentration and fluorescence indices but the lowest percentage of carbohydrates. Regardless of sampling locations, the HMW-DOM fractions contained higher abundances of aromatic DOM, carbohydrates and protein-like substances, but lower abundance of fulvic acid-like substances compared to those in the LMW fractions. Metal titration experiments coupled with the excitation-emission matrix (EEM)-parallel factor (PARAFAC) modeling revealed that the quenching of the PARAFAC-derived fluorescent components was more pronounced in the presence of Cu(II) compared to Pb(II). Humic-like components emerged as a superior model, exhibiting higher binding affinities for Cu(II) than protein-like substances, while the opposite trend was observed for Pb(II). In samples obtained from Lakes Erhai and Kokonor, the condition stability constants (Log KM) for the binding of both Cu(II) and Pb(II) with the HMW-DOM fraction were higher than those with the LMW-DOM fraction. Conversely, a contrasting trend was observed for Lake Chaka. This study highlighted the heterogeneity in spectral properties and metal-binding behaviors of natural DOMs, contributing to an improved understanding of the molecular interactions between DOM components and metal ions and their environmental fate in aquatic ecosystems.

Investigation of the Distribution and Binding Affinity of Copper to Size-Fractioned Dissolved Organic Matter (DOM) in a Constructed Wetland

This study investigated the distribution and binding affinity of dissolved copper (Cu) and organic carbon (OC) in size-fractioned dissolved organic matter (DOM) in a constructed wetland (CW). Two sites were studied: one at the inflow (P-1) and one within the wetland (P-2). The DOMs (&lt;0.45 μm) were separated into six size fractions using a cross-flow ultrafiltration system. In the wetland (P-2), the concentrations of dissolved organic carbon (DOC) increased while the concentrations of Cu decreased. The high molecular weight fraction (1 kDa–0.45 μm, HMW) contained most of the OC mass (57.4–71.2% averages). On the other hand, Cu was almost equally distributed in HMW and low molecular weight fractions (&lt;1 kDa, LMW) with mean HMW percentages of 50.3–51.3%. The mean Cu binding affinity to DOM ratios (CuBADOM) was 74.9 ± 24.0 μmol/g-C at site P-1 and 17.3 ± 2.6 μmol/g-C at site P-2. The CuBADOM ratios were decreased in wetlands of bulk and size-fractioned DOM (p &lt; 0.001 to p = 0.073). The SUVA254 values for bulk DOM solution were 2.54 ± 0.15 and 1.68 ± 0.18 L/mg-C/m, and humidification index (HIX) values were 1.74 ± 0.16 and 2.09 ± 0.19 for sites P-1 and P-2, respectively. Optical indicators suggested that the wetland process decreased aromaticity but increased the humification degree of DOM. Furthermore, the CuBADOM ratios positively correlated with SUVA254 and HIX within the constructed wetland DOM but not in the influent DOM. Understanding the Cu distribution and binding affinity to size-fractioned DOM makes it possible to develop strategies to mitigate the potential effects of copper pollution in wetlands.