Fourier Transform Infrared Test Research Articles

Elucidating the interaction of propofol and serum albumin by spectroscopic and docking methods

The interaction of human serum albumin (HSA) with propofol has been studied by fluorescence spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, circular dichroism (CD) spectroscopy and docking methods. A gradual decrease in Stern–Volmer quenching constants with the increase in temperature showed the static mode of fluorescence quenching. The obtained binding constant (KA) was 8.18×105M−1. Gibbs free energy (ΔG), enthalpy (ΔH) and entropy (ΔS) changes were calculated, which revealed that the reaction is spontaneous, exothermic and hydrophobic force driven. FT-IR test revealed conformational changes of the protein and destruction of H-bonding upon interaction. Moreover, propofol induced a decrease in α-helical contents probably with increment of random coils or/and β-sheets of HSA, as observed from the far-UV CD spectra. Molecular docking and site probing study depicted that propofol fits into the hydrophobic pocket close to Sudlow site I in domain IIA of HSA. The present study will be helpful in understanding the binding mechanism of propofol and associated stability and conformational changes.

Fourier Transform Infra-Red Spectroscopy and Chemical Resistance of Untreated and Alkali Treated Coconut Leaf Sheath Fiber Reinforced Polymer Composites

In these days, natural fiber and its composites are gaining more interest and utilization of biofibers has increased because of growing concern towards economic, environmental and conservation issues. The present study investigates, Fourier Transform Infra-red (FTIR) spectroscopy and chemical resistance properties on the coconut leaf sheath (CLS) reinforced Phenol Formaldehyde (PF) composites. Naturally woven coconut leaf sheath composites were prepared in both NaOH treated and untreated forms with volume fraction of 60% of sheath and 40% of Phenol Formaldehyde resin. CLS were chemically treated using 5% of NaOH and composite boards were made using a hydraulic hot press at 140°C. FTIR test was conducted for both treated and untreated CLS fiber composites. And it was found that, due to chemical treatment of fibers reduction in lignin and hemicellulose content was observed. The chemical resistance of alkali treated and untreated CLS composites were measured by chemical absorption and chemical thickness swelling methods. Treated and untreated composite samples were then placed in 1N of saline, Hcl, HNO3 and H2SO4 separately. It is also observed that, saline and Hcl have been absorbed more in case of untreated composites and HNO3and H2SO4 is absorbed more in treated composites. These tests were done to know that, these composites can be used in manufacturing products which gives better chemical resistance.

Synergistic effects of sepiolite on the flame retardant properties and thermal degradation behaviors of polyamide 66/aluminum diethylphosphinate composites

The effect of sepiolite as a synergistic agent on the flame retardancy and thermal degradation behavior of polyamide 6,6 (PA66)/aluminium diethylphosphinate (AlPi) composites were investigated by limiting oxygen index (LOI), vertical burning (UL-94) tests, thermogravimetric analysis/infrared spectrometry (TG-IR) and cone calorimeter tests (cone). The morphologies and the chemical compositions of char residue were investigated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectra. The results demonstrated that the specimens with the thickness of 1.6 mm passed UL-94 V-0 flammability rating and the LOI value was as high as 33.4% when the loading amount of AlPi was 14 wt %. However, when sepiolite (3 wt % based on flame retardant) incorporated into the AlPi/PA66 composites and the total loading amount of AlPi/sepiolite mixture was only 10 wt %, the samples with the thickness of 1.6 mm successfully passed UL-94 V-0 flammability rating and the LOI value reached 32.5%. It was deduced that the combination of AlPi with sepiolite possessed an evident synergistic effect and enhanced the flame retardant efficiency for PA66 materials. The TG-IR and FTIR tests of the char residues revealed that the incorporation of sepiolite changed the degradation process of the PA66/AlPi system and possibly formed diethylphosphinic acid, siliconphosphate and magnesiumphosphate in condense phase, which enhanced the thermal stability and char yield of PA66 materials at high temperature. The results of cone tests revealed that AlPi combined with sepiolite efficiently reduced the fire behavior parameters, such as heat release rate (HRR), total heat release (THR) and so on. The digital photographs and morphological structures of char residues demonstrated that sepiolite benefited to the formation of more sufficient, compact and homogeneous char layer on the materials surface during burning. The analysis of XPS indicated that sepiolite promoted the formation of flame retardant elements rich char layer on the materials surface, which increased the strength of the char layer and enhanced the flame retardancy of PA66 polymeric materials.

Preparation of Al2O3/PU/PVDF composite membrane and performance comparison with PVDF membrane, PU/PVDF blending membrane, and Al2O3/PVDF hybrid membrane

In this study, Al2O3/polyurethane/polyvinylidene fluoride (Al2O3/PU/PVDF) composite membrane was prepared via the thermally induced phase separation process. Pure PVDF membrane, PU/PVDF blending membrane, and Al2O3/PVDF hybrid membrane were also prepared for comparison. These membranes were characterized by means of contact angle test, mechanical properties test, Fourier transform infrared (FT-IR) spectroscopy, atomic force microscopy (AFM), and differential scanning calorimetry (DSC). In addition, the separation performance of membranes was evaluated in terms of water flux and rejection ratio. The results showed that the Al2O3/PU/PVDF composite membrane had better hydrophilicity and mechanical properties compared with pure PVDF membrane, PU/PVDF blending membrane, and Al2O3/PVDF hybrid membrane. FT-IR test confirmed the introduction of the hydrophilic groups, such as hydroxyl and carbonyl, which are responsible for the enhancement of the hydrophilicity of Al2O3/PU/PVDF composite membrane. The AFM results showed that the Al2O3/PU/PVDF composite membrane had the lowest roughness, manifesting its improved anti-fouling properties. Furthermore, DSC results revealed that the crystallinity of PVDF polymer matrix decreased with the introduction of the functionalized Al2O3 nanoparticles.

Preparation of porous nylon 6 fiber via electrospinning

Porous nylon‐6 fibers were obtained by electrospinning of ultra‐high molecular polyamide 6 (UHMW‐PA6). First, UHMW‐PA6/calcium formate composite nanofibers were prepared as precursors by electrospinning UHMW‐PA6 solutions containing different contents of calcium formate particles. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the surface morphology and inner structure of composite nanofibers. It was found that calcium formate particles were distributed both inside and on the surface of nanofibers. Fourier transform infrared (FTIR), differential scanning calorimetry, and thermal gravimetric analysis (TGA) were used to study the structure and properties of these nanofibers. Then, porous UHMW‐PA6 nanofibers were obtained by soaking the electrospun web in water for 24 h, to remove calcium formate particles. The removal of calcium formate particles was confirmed using FTIR and TGA tests. SEM and TEM observations revealed the formation of porous structure in these nanofibers. In addition, CaCl2 was used instead of calcium formate to prepare the UHMW‐PA6 nanoporous fiber. POLYM. ENG. SCI., 55:1133–1141, 2015. © 2014 Society of Plastics Engineers

A novel polybenzoxazine containing styrylpyridine structure via the Knoevenagel reaction

ABSTRACTIn this article, a kind of styrylpyridine‐containing polybenzoxazine was obtained via the Knoevenagel reaction between benzaldehyde and methylpyridine groups. The benzoxazine monomer (MPBC) containing the benzaldehyde and methylpyridine groups was synthesized firstly and its structure was characterized by Fourier transform infrared (FTIR) spectra, 1H NMR and 13C NMR. With the aid of differential scanning calorimetry, FTIR, and photoluminescent tests, the interesting curing behaviors were probed. The results showed that the ring‐opening polymerization occurred at lower temperature, and the Knoevenagel reaction further took place at elevated temperature. The amine and phenol moieties were bonded together to form the styrylpyridine structure. Due to these special crosslinking structures, the corresponding polybenzoxazine exhibited excellent thermal stability, and had a special high char yield of 74.5%. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40823.

Effects of Interaction Conditions on Internal Network Structure of Crumb Rubber–Modified Asphalts

Investigation of the effect of interaction conditions on the development of the internal network structure of crumb rubber–modified asphalt (CRMA) was carried out. Enhancements in the CRMA physical properties, such as stiffness, expressed in the complex modulus ( G*), and elasticity, expressed in the phase angle (δ), as a result of the development of three-dimensional (3-D) entangled network structure were achieved. These results came from controlling the interaction conditions of CRMA synthesis to regulate the crumb rubber–modified (CRM) components that were responsible for the formation of the 3-D entangled network structure. Dissolution tests and thermogravimetric analysis (TGA) were performed on the extracted CRM particles after inter action with asphalt. A Fourier transform infrared (FTIR) spectroscopy investigation was performed on the liquid phase asphalt after separation of CRM. FTIR was used to investigate the state of the released CRM components, previously calculated by TGA, which were responsible for the formation of the 3-D network structure. Use of rheological, TGA, and FTIR tests confirmed that the enhancement of the CRMA's G* and δ was dependent on the dissolution or release of CRM components under specific interaction conditions that led to the existence of a 3-D network structure within the liquid phase of the CRMA. The development of the 3-D entangled network structure in CRMA was found to be attributable to the release of most of the CRM natural and synthetic rubber components under specific interaction conditions. Enhancements in the CRMA G* and δ provided improved rutting resistance and alleviated permanent deformation problems.

Short Term and Long Term Aging Effects of Asphalt Binder Modified with Montmorillonite

Nowadays, most of asphalt used in pavement construction is produced from petrochemicals through refining process of crude oil. When evolves with time, asphalt become stiffer and brittle due aging process. In this paper, the rheological effect of short-term and long-term oxidative ageing of asphalt binder modified with montmorillonite (MMT) was studied using SuperpaveTM rotational viscometer (RV), dynamic shear rheometer (DSR) and Fourier transform infrared (FTIR) instruments. The results indicate the addition of MMT increased the viscosity. Based on dynamic mechanical analysis with DSR, MMT modified binder has a potential to increase rutting and fatigue resistance. In terms of FTIR test, it appears that the chemical bonding had changed in the asphalt binder before and after RTFO and PAV aging which suggest that the additional of MMT could delay the aging process.

Preparation and Application of Low Molecular Weight Chitosan Nanoparticle as a Textile Finishing Agent

Due to the advantages of both the chitosan and the nanomaterial, chitosan nanoparticle has a broad application in a lot of fields, such as medicine carrier, food process, cosmetics and agriculture protect. And there also appears a lot of research about chitosan nanoparticle in textile finishing in recent years. In former research, steady state chitosan nanoparticles were prepared by ionotropic gelation method in dispersion system. In this paper, in order to confirm the preparation of low molecular weight chitosan nanoparticle, it was also characterized by Fourier Transform Infrared (FT-IR) Spectrometry, X-ray diffraction (XRD) and Transmission Electron Microscope (TEM). Focusing on the application value, chitosan nanoparticles dispersion solution were used as one kind of textile finishing agent to modifyB.morisilk fabrics in order to realize the functionalization of silk fabrics. The wrinkle resistance and bacteria repellency of silk fabrics were tested in the paper. The results showed that chitosan nanoparticles were successfully prepared and confirmed accrording to the XRD, FT-IR and TEM tests. In addition, compared with the ordinaryB. morisilk fabric and theB. morisilk fabric treated with chitosan accordingly, theB. morisilk fabrics treated with chitosan nanoparticle dispersion system had better wrinkle resistance and bacteria repellency.

PENGARUH PENGGUNAAN LARUTAN ALKALI DALAM UJI FOURIER TRANSFORM INFRARED PADA KOMPOSIT TERMOPLASTIK BERPENGISI SERBUK SERABUT KELAPA

Composite is a combination of two different materials to obtain a material with physical and mechanical properties are better than any of its constituent parts. One of the composite types which is much produced is composite with powdered natural fiber filler. This research uses the used polypropylene matrix and powdered coconut fiber as filler treated with Sodium Hydroxide (NaOH). The ratio of the matrix and the filler is 85:15. This ratio is the optimum value obtained by the composite tensile strength with ratio 100:0, 95:5, 90:10 and 85:15. The purpose of this study was to determine the effect of the use of an alkaline solution of the resulting composite properties especially towards lignin level, with Fourier Transform Infrared (FTIR) test on composite material used polypropylene with powdered coconut fiber as filler. The method used in the manufacture of composites is the method of extrusion. The matrix form of the used polypropylene mixed with coconut fiber powder that has been soaked with NaOH, then mixed in a container, then put into the extruder operating temperature 1750C, printed using a hot press at a temperature of 1750C, and cut into pieces appropriate testing. The results showed that the optimum processing occurs with NaOH for 2 days. The FTIR test result, showed that –OH cluster switch occured, be seen from the shifting of absortion band from 3417.04 cm-1 to 3476.84 cm-1 for the powdered coconut fiber and the shifting of absortion band from 3343.75 cm-1 to 3192.33 cm-1 for the composite, which is the –OH cluster of the lignin.

FOLIC ACID-FUNCTIONALIZED GOLD AND SILVER NANOPARTICLES: THEIR CYTOTOXIC EFFECT ON CANCEROUS MYELOID CELLS WITH MICROWAVE IRRADIATION

Introduction: Metal nanoparticles such as gold and silver nanoparticles have attracted much interest during the last decades for their special chemical and physical properties. Gold and silver nanoparticles can be functionalized with active biologic moieties like antibodies, drugs and chemicals, enabling them to react with specific cells. Furthermore, penetration and cytotoxic effects of nanoparticles can be increased by electromagnetic waves such as infrared, ultraviolet, radiofrequency and microwave. Aim: The aim of this study was to evaluate the rate of cell cytotoxicity induced by folic acid-functionalized gold and silver nanoparticles with and without microwave irradiation on cancer cells from patients with acute myeloid leukemia (AML). Method: Patients with known AML (M1, M2, M3 and M4), all recently diagnosed by histopathology, special stains and immunohistochemistry, and 4 normal persons were enrolled in the study. The blood mononuclear cell fraction was separated, so that the final concentration of neoplastic myeloid cells and normal mononuclear cells in each tube was adjusted to about 400 cells/μL. For preparation of folate-functionalized gold and silver nanoparticles, folic acid was dissolved in deionized water, added to 1 mM HAuCl4 and 1 mM AgNO3 solution, and incubated at 50°C for 8 h. Scanning electron micrographs, ultraviolet-visible spectrophotometer and Fourier transform infrared (FTIR) were used for confirmation of the synthesis of functionalized nanoparticles. After preparation, nanoparticles were added to cancerous and normal cell suspensions, and then incubated at 37°C for 1 h. Another experiment was carried out in the same way but with exposure to microwave irradiation for 10 s so that its temperature reached at 50°C, and then incubated at 37°C for 1 h, after which cell cytotoxicity was evaluated with MTT test. All of the tests were duplicated, and paired t-test was used to compare the mean absorbance read-out in each of the above-mentioned groups of wells. Results: The sizes of functionalized gold and silver nanoparticles were approximately 25 nm to 32 nm. After synthesis of functionalized nanoparticles, the tubes containing HAuCl4 turned to red color, and the peak absorbance for gold nanoparticles was at 520 nm. For AgNo3 , it turned to yellow color with a peak absorbance at 420 nm. FTIR test showed connection of folic acid moieties to gold and silver surfaces. This study showed that functionalized gold nanoparticles were more toxic than functionalized silver nanoparticles on cancer and normal cells. Also, microwave irradiation was more synergic with functionalized gold nanoparticles. Furthermore, the most effectiveness score was 2.87 for functionalized silver nanoparticles without microwave irradiation and the minimum effectiveness score was 2.20 for functionalized silver nanoparticles with microwave. Conclusion: This study clearly demonstrated that although functionalized gold nanoparticles have high toxicity to cells, but silver nanoparticles without microwave irradiation are more effective because of less cytotoxic effect on normal cells.

Mass Transfer Through Microwave-Treated Fir Wood (Abies alba L.): A Gymnosperm Species with Torus Margo Pit Membrane

In this research, the effect of microwave pretreatment on mass transfer coefficients of Abies alba L. was investigated. For this purpose, wood specimens with dimensions of 340 × 100 × 50 mm and average moisture content of 50% were exposed to a microwave with 2450 MHz frequency for time durations of 7 and 10 minutes. After drying, the air permeability and water vapor diffusion coefficients were measured through the longitudinal and radial directions of the specimens and results were analyzed using chemical composition measurement, FT-IR (Fourier Transform Infrared) test, and SEM (Scanning Electron Microscope) studies. The FT-IR study of pretreated samples showed no specific change, although SEM photographs proved the hydrolyzation of bordered pits as a result of microwave heating. Results revealed that microwave pretreatment had no effect on the longitudinal permeability and diffusion coefficients. In contrast, the radial mass transfer rates were improved due to the microwave pretreatment. Although the microwave pretreatment had no noticeable influence on the chemical compositions of the treated specimens, the torus of some bordered pits was hydrolyzed due to microwave radiation.

Influence of Composite Antioxidants to Asphalt Anti-Aging Performance

The durability of the asphalt is of great significance to its pavement performance, so more and more effort has been addressed on improving the asphalt anti-aging performance. In this work, the effects of antioxidant type and formula on the asphalt anti-aging performance were investigated through various measurements before and after aging process including needle penetration, softening point and ductility. Furthermore, the aging mechanism was analyzed via the FTIR (Fourier Transform Infrared) test. As a result, composite antioxidant can observably improve aging resistance of the asphalt. When the formula is 1.2%B+1.5%C+1.2%D, the needle penetration, ductility, softening point are as follows: 39.4×10-1mm, 148.1cm, 52.1°C. FTIR shows that carbonyl absorption peak reduced. Moreover, composite antioxidant can be more stable improvement of asphalt aging effect due to the cooperativity.

A Study of FTIR, Thermal Properties and Natural Weathering Test on NBRVirgin/Recycled with SBR Blends

The effects of styrene butadiene rubber/virgin acrylonitrile butadiene rubber (SBR/NBRv) blends and styrene butadiene rubber/recycled acrylonitrile butadiene rubber (SBR/NBRr) blends on properties such Fourier transform infrared (FTIR), thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC) were carried out. Results indicated that, based on intensity of amine peak from FTIR at 85/15 blend ratio (R15) revealed optimum formation of crosslink between SBR and NBR either using virgin or NBRr. TG thermograms of SBR/NBRv blends of all ratios showed better onset thermal stability than SBR/NBRr blends. The change in the horizontal baseline from high to low energy level occurred in virgin NBR blends because the amount of reactive sites available in virgin NBR is higher compared to NBRr. Meanwhile NBRr blends showed Tc because the amount of crosslink occurred in these blends were slightly lower than NBRv blends. Up to 25 phr of NBRr, the tensile strength and elongation at break (Eb) retention of SBR/NBRv blends was better than SBR/NBRr blends after 6 months' weathering test except for M100. The scanning electron microscopy on the surface of both blends after 6 months exposure indicated that the severity of the crack was minimal for SBR/NBRr blends compared to SBR/NBRv particularly at 50/50 blend ratio designated the SBR/NBRr blends that contained more NBRr particles could reduce the degradation towards natural weathering.

Preparation and properties of polyamide 6 fibers prepared by the gel spinning method

AbstractPolyamide 6 (PA6) fibers were prepared by CaCl2 complexation and the gel spinning technique. PA6 was partially complexed with CaCl2 for the purpose of suppressing interchain amide group hydrogen bonding. The fibers were characterized with scanning electron microscopy, X‐ray diffraction (XRD), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR) spectroscopy. In the gel spinning process, a mixed tetrachloroethane and chloroform solution was chosen as the coagulation bath after a comparison of different types of solutions. From our investigation of the morphology, structure, and mechanical properties of gel‐spun and hot‐drawn fibers, it was indicated that the modulus and tensile strength increased with increasing draw ratio, the orientation of the fibers was improved, and the cross section of the PA6 gel fibers became more smooth and tight. The results from the XRD, DSC, and FTIR tests indicated that calcium metal cations complexed with the carbonyl oxygen atoms of PA6. The maximum modulus and tensile strength values obtained in this study were 28.8 GPa and 413 MPa, respectively, at a draw ratio of 8. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Investigation of catalytic mechanism of formaldehyde oxidation over three-dimensionally ordered macroporous Au/CeO2 catalyst

A colloidal crystal template method coupled with a precursor complexion process was developed to create three-dimensionally ordered macroporous (3DOM) Au/CeO2 catalyst. The resultant Au/CeO2 catalyst possesses well-defined 3DOM structure, and shows enhanced catalytic performance for formaldehyde (HCHO) oxidation with 100% HCHO conversion at ∼75°C. The catalytic mechanism of HCHO catalytic oxidation over 3DOM Au/CeO2 catalyst was systematically investigated by means of gas chromatograph (GC), H2-temperature programmed reduction (H2-TPR), temperature programmed surface reaction (TPSR), CO2-temperature programmed desorption (TPD), and Fourier transform infra-red (FT-IR) spectroscopy. GC results indicate that HCOOH intermediate is generated during HCHO catalytic oxidation. TPD and TPSR tests show that the weak absorption ability of CO2 over 3DOM Au/CeO2 catalyst and the existence of Au active species in ionic and metallic states in 3DOM Au/CeO2 catalyst largely improve the catalytic activity, favoring the enhanced HCHO catalytic oxidation. FT-IR tests prove that the carbonate and hydrocarbonate formed on the surface of 3DOM Au/CeO2 catalyst during HCHO catalytic oxidation may account for its deactivation. Based on the above investigation, a new catalytic mechanism of enhanced HCHO catalytic oxidation over 3DOM Au/CeO2 catalyst is proposed. The mechanism may afford the scientific guidance for preparing high efficiency oxide supported noble metal catalysts and present a solution for solving their deactivation problem.

Synthesis and Structural Characterization of Graphene-Based Membranes

A stable hydrosol of graphene was synthesized by oxidation reduction and then a flow assembly of this graphene was used to form a graphene-based membrane by vacuum extraction filtering method. X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, particle size analysis, and scanning probe microscopy (SPM) were used to characterize the crystal structure, granularity, and characteristic change of the molecular spectrum of the samples in the reaction. FTIR tests show that the structural layer of graphite during the oxidation process bonds to a large number of functional groups and parts of these stable functional groups remain on the reduced structural layer of graphene. X-ray diffraction results show that the peaks of the graphite oxide shift to lower angles, become broader and the original graphite peak disappears. Suspensions of graphene oxide form condensed matter and graphene flocculating constituent during film deposition. Particle size analysis and SPM tests show that the particle sizes of the graphene oxide sheets that are dispersed in water show a tailing peak and a broad distribution while the graphene sheets show a singlet, narrower distribution, and smaller dimensions. Raman results show that during oxidation and reduction, the D peak and G peak of the samples gradually extend, ID/IG increases gradually and the degree of sample disorder increases. On the basis of the above analyses, the structural characteristics of the samples in the reaction are summarized.

A facile approach to fabricate porous nylon 6 nanofibers using silica nanotemplate

AbstractPorous Nylon 6 nanofibers were prepared using silica nanoparticles as the template. Firstly, Nylon 6/silica composite nanofibers were prepared as precursors by electrospinning Nylon 6 solutions containing different contents of silica nanoparticles. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the surface morphology and the inner structure of composite nanofibers; where it was found that silica nanoparticles were distributed both inside and on the surface of nanofibers. Analytical techniques [Fourier transform infrared (FTIR), differential scanning calorimetry, thermal gravimetric analysis (TGA), and wide‐angle X‐ray diffraction) were used to study the structure and properties of these composite nanofibers. The glass transition, melting, and crystallization processes of the fibers were affected by the addition of silica nanoparticles. Secondly, porous Nylon 6 nanofibers were obtained by removing silica nanoparticles via hydrofluoric acid treatment. The removal of silica nanoparticles was confirmed using FTIR and TGA tests. SEM and TEM observations revealed the formation of the porous structure in these nanofibers. After the formation of the porous structure, Brunauer–Emmett–Teller specific surface areas of nanofibers were increased as compared to solid Nylon 6 and composite nanofibers. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011

Separation and identification of multicomponent mixture by thin-layer chromatography coupled with Fourier transform–infrared microscopy

A fast and convenient method based on coupled thin-layer chromatography (TLC) with Fourier transform infrared (FTIR) microscopy has been established for separation and identification of multicomponent mixtures. In this study, the method was developed and consummated with more perfect TLC spots transferral process and consistent FTIR testing conditions. A newly developed technique, solid-phase extract (SPE) was introduced for sample pre-treatment instead of using traditional column chromatography. It is a new field for SPE that has already been widely applied in many other fields. It not only overcomes the backwards (low separation efficiency, time consuming and solvent consumption) of column chromatography but also makes it much easier to choose an optimum TLC sheet and to set suitable TLC loading. With all the above-mentioned modifications and supplements, the analytical method of coupled TLC with FTIR microscopy for separation and identification of multicomponent mixtures becomes more convenient and more efficient. In addition, a very complex sample (a die-cast release agent) was used as an example to demonstrate the technique.