Amomum putrescens D. Fang is a member of the genus Amomum Roxb. (Zingiberaceae), which is found in Vietnam and China. To date, the chemical components and bioactivities of A. putrescens essential oils (EOs) have not been investigated. In this work, the rhizome and pseudo-stem EOs of A. putrescens from Northern Vietnam were extracted by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). These EOs were evaluated for their biological effects by inhibiting α-amylase and tyrosinase enzymes. Regarding the chemical composition profile, thirty-two and thirty-six volatile components accounted for 97.88% and 98.37% of the rhizome and pseudo-stem EOs, respectively. The main constituents in these EOs were β-pinene, myrtenyl acetate, α-pinene, and trans-pinocarvyl acetate. Notably, β-phellandrene (5.87%) was found only in the pseudo-stem EO but absent in the rhizome EO. In the enzyme inhibitory test, both EOs revealed inhibitory activity against α-amylase (IC50 = 122.37 ± 26.55 μg/mL for rhizomes and IC50 = 108.48 ± 0.44 μg/mL for pseudostems) and tyrosinase (IC50 = 910.85 ± 70.40 μg/mL for pseudo-stems), while rhizome EO had no inhibitory effect on the tyrosinase enzyme. The evidence explored in the present work will lay a scientific foundation for further research on the bioactivities and the extracted components of different parts of A. putrescens, especially the volatile oil components.
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