Fossil Samples Research Articles

Evaluating developmental shape changes in Homo antecessor subadult facial morphology

The fossil ATD6-69 from Atapuerca, Spain, dated to ca. 900 ka (thousands of years ago) has been suggested to mark the earliest appearance of modern human facial features. However, this specimen is a subadult and the interpretation of its morphology remains controversial, because it is unclear how developmental shape changes would affect the features that link ATD6-69 to modern humans. Here we analyze ATD6-69 in an evolutionary and developmental context. Our modern human sample comprises cross-sectional growth series from four populations. The fossil sample covers human specimens from the Pleistocene to the Upper Paleolithic, and includes several subadult Early Pleistocene humans and Neanderthals. We digitized landmarks and semilandmarks on surface and CT scans and analyzed the Procrustes shape coordinates using multivariate statistics. Ontogenetic allometric trajectories and developmental simulations were employed in order to identify growth patterns and to visualize potential adult shapes of ATD6-69. We show that facial differences between modern and archaic humans are not exclusively allometric. We find that while postnatal growth further accentuates the differences in facial features between Neanderthals and modern humans, those features that have been suggested to link ATD6-69's morphology to modern humans would not have been significantly altered in the course of subsequent development. In particular, the infraorbital depression on this specimen would have persisted into adulthood. However, many of the facial features that ATD6-69 shares with modern humans can be considered to be part of a generalized pattern of facial architecture. Our results present a complex picture regarding the polarity of facial features and demonstrate that some modern human-like facial morphology is intermittently present in Middle Pleistocene humans. We suggest that some of the facial features that characterize recent modern humans may have developed multiple times in human evolution.

Morphometric analysis of molars in a Middle Pleistocene population shows a mosaic of ‘modern’ and Neanderthal features

Previous studies of upper first molar (M1) crown shape have shown significant differences between Homo sapiens and Homo neanderthalensis that were already present in the European Middle Pleistocene populations, including the large dental sample from Atapuerca-Sima de los Huesos (SH). Analysis of other M1 features such as the total crown base area, cusp proportions, cusp angles and occlusal polygon have confirmed the differences between both lineages, becoming a useful tool for the taxonomic assignment of isolated teeth from Late Pleistocene sites. However, until now the pattern of expression of these variables has not been known for the SH sample. This fossil sample, the largest collection from the European Middle Pleistocene, is generally interpreted as being from the direct ancestors of Neanderthals, and thus is a reference sample for assessing the origin of the Neanderthal morphologies. Surprisingly, our study reveals that SH M(1) s present a unique mosaic of H. neanderthalensis and H. sapiens features. Regarding the cusp angles and the relative occlusal polygon area, SH matches the H. neanderthalensis pattern. However, regarding the total crown base area and relative cusps size, SH M(1) s are similar to H. sapiens, with a small crown area, a strong hypocone reduction and a protocone enlargement, although the protocone expansion in SH is significantly larger than in any other group studied. The SH dental sample calls into question the uniqueness of some so-called modern traits. Our study also sounds a note of caution on the use of M(1) occlusal morphology for the alpha taxonomy of isolated M(1) s.

Toward an accurate taxonomic interpretation ofCarexfossil fruits (Cyperaceae): A case study in sectionPhacocystisin the Western Palearctic

Despite growing interest in the systematics and evolution of the hyperdiverse genus Carex, few studies have focused on its evolution using an absolute time framework. This is partly due to the limited knowledge of the fossil record. However, Carex fruits are not rare in certain sediments. We analyzed carpological features of modern materials from Carex sect. Phacocystis to characterize the fossil record taxonomically. We studied 374 achenes from modern materials (18 extant species), as well as representatives from related groups, to establish the main traits within and among species. We also studied 99 achenes from sediments of living populations to assess their modification process after decay. Additionally, we characterized 145 fossil achenes from 10 different locations (from 4-0.02 mya), whose taxonomic assignment we discuss. Five main characters were identified for establishing morphological groups of species (epidermis morphology, achene-utricle attachment, achene base, style robustness, and pericarp section). Eleven additional characters allowed the discrimination at species level of most of the taxa. Fossil samples were assigned to two extant species and one unknown, possibly extinct species. The analysis of fruit characters allows the distinction of groups, even up to species level. Carpology is revealed as an accurate tool in Carex paleotaxonomy, which could allow the characterization of Carex fossil fruits and assign them to subgeneric or sectional categories, or to certain species. Our conclusions could be crucial for including a temporal framework in the study of the evolution of Carex.

Modelling distributions of fossil sampling rates over time, space and taxa: assessment and implications for macroevolutionary studies

Summary Observed patterns in the fossil record reflect not just macroevolutionary dynamics, but preservation patterns. Sampling rates themselves vary not simply over time or among major taxonomic groups, but within time intervals over geography and environment, and among species within clades. Large databases of presences of taxa in fossil‐bearing collections allow us to quantify variation in per‐collection sampling rates among species within a clade. We do this separately not just for different time/stratigraphic intervals, but also for different geographic or ecologic units within time/stratigraphic intervals. We then re‐assess per‐million‐year sampling rates given the distributions of per‐collection sampling rates We use simple distribution models (geometric and lognormal) to assess general models of per‐locality sampling rate distributions given occurrences among appropriate fossiliferous localities. We break these down not simply by time period, but by general biogeographic units in order to accommodate variation over space as well as among species. We apply these methods to occurrence data for Meso‐Cenozoic mammals drawn from the Paleobiology Database and the New and Old Worlds fossil mammal database. We find that all models of distributed rates do vastly better than the best uniform sampling rates and that the lognormal in particular does an excellent job of summarizing sampling rates. We also show that the lognormal distributions vary fairly substantially among biogeographic units of the same age. As an example of the utility of these rates, we assess the most likely divergence times for basal (Eocene–Oligocene) carnivoramorphan mammals from North America and Eurasia using both stratigraphic and morphological data. The results allow for unsampled taxa or unsampled portions of sampled lineages to be in either continent and also allow for the variation in sampling rates among species. We contrast five models using stratigraphic likelihoods in different ways to summarize how they might affect macroevolutionary inferences.

The Cuban crocodile (Crocodylus rhombifer) from late Quaternary fossil deposits in the Bahamas and Cayman Islands

Late Quaternary fossil deposits from The Bahamas, Cayman Islands, and Cuba contain fossils of the Cuban crocodile Crocodylus rhombifer. This species survives today only in Cuba and Isla de la Juventud (formerly Isla de Pinos); the populations in The Bahamas and Cayman Islands are locally extinct. Large fossil samples, including skulls, are known from underwater caves (blue holes) on Abaco in the northern Bahamas and organic peat deposits on Grand Cayman. Diagnostic cranial characters shared by the fossil crocodile skulls from Abaco and Grand Cayman and recent skulls of C. rhombifer from Cuba are: short, broad, and deep rostrum; prominent orbit; concave interorbital region and cranial roof; strong ridge on internal margin of the orbit and lateral margin of the cranial table; large, rounded protuberance on the posterolateral corner of the squamosal; premaxillary-maxillary suture on the palate transverse at the level of the first maxillary tooth; and 13 teeth in the maxilla. Using a ratio derived from living crocodylians of head length (premaxilla to parietal) to total length of about 1:7.2, approximate total lengths for fossil specimens of C. rhombifer from Abaco range from 1.3–2.3 m (mean 1.9 m) and Grand Cayman from 1.6–2.0 m (mean 1.7 m). Quaternary crocodylian remains are known from many other islands in The Bahamas, including Acklins, Crooked Island, Eleuthera, Grand Bahama, Mayaguana, New Providence, and San Salvador; however, most of these fossils are not complete enough for a species identification. Shells of an extinct species of the land tortoise Chelonoidis from Abaco and Mayaguana with crocodylian bite marks, jaws and teeth of capromyid rodents from Grand Cayman that appear to have been digested by a crocodylian, and data from carbon (13C/12C) isotopes derived from crocodile bones, as well as the lack of large freshwater vertebrates, suggest that C. rhombifer in The Bahamas and Cayman Island had a diet primarily consisting of terrestrial vertebrates. Evidence from stomach contents and behavioral ecology of extant Cuban crocodiles from Cuba further supports the terrestrial feeding habits of C. rhombifer. Radiocarbon (14C) dates on crocodile postcranial bones from Sawmill Sink and Dan’s Cave on Abaco range from 2,780–3,680 years Before Present (BP). Radiocarbon dates on peat associated with fossils of C. rhombifer from the Crocodile Canal site on Grand Cayman range from 375–860 years BP. These dates confirm that Cuban crocodiles survived into the late Holocene on Abaco and into the historic period on Grand Cayman. Evidence from a variety of sources, including radiocarbon dates, fossil and archaeological sites, and historical records confirms that the local extinction of crocodiles in The Bahamas and Cayman Islands occurred within the past 500 years, probably resulting from overhunting by humans. Fossils of C. rhombifer on Grand Cayman and Abaco, and probably throughout The Bahamas, verify that the Cuban crocodile was considerably more widespread in the West Indies during the Late Quaternary.

Multivariate analysis of modern and fossil pollen data from the central Tianshan Mountains, Xinjiang, NW China

To interpret past vegetation and climate changes from pollen data, we need to reveal the degree of similarity between modern analogues and fossil pollen spectra, which would help us predict the future climate and vegetation. Ninety surface pollen samples across six vegetation zones along an altitudinal gradient from 460 to 3510 m and 44 fossil samples at Caotan Lake were collected in the central Tianshan Mountains, northern Xinjiang, China. Discriminant analyses results, fossil pollen and phytolith assemblages were then used to reconstruct palaeovegetation and palaeoclimate in the area. The 90 surface samples were divided into six pollen zones (alpine cushion, alpine and subalpine meadow, montane Tianshan spruce forest, forest-steppe ecotone, Artemisia desert, typical desert), corresponding to the major vegetation types in the area. These zones follow a climatic gradient of increasing precipitation with increasing elevation. Paleovegetation reconstructed from 44 fossil pollen assemblages through discriminant analysis reflects the regional vegetation shifted from typical desert to Artemisia desert since 4640 cal. year BP in the Caotan Lake wetland. The fossil pollen and phytolith record also reveal the arid climate has not fundamentally changed in the period. But a dry-wet-dry local climate oscillation since 2700 cal. year BP has a fundamental influence on local wetland vegetation dynamics and peat accumulation of the Caotan wetland. Modern wetland landscape and surface pollen assemblages from the Ebinur Lake Wetland Nature Reserve provide further evidence for ferns and Betula growing in the Caotan Lake wetland during the historical period.

Waking the Dead: Morphological and Molecular Characterization of Extant †Posoniella tricarinelloides (Thoracosphaeraceae, Dinophyceae)

The Thoracosphaeraceae are dinophytes that produce calcareous shells during their life history, whose optical crystallography has been the basis for the division into subfamilies. To evaluate the validity of the classification (mainly applied by palaeontologists), living material of phylogenetic key species is necessary albeit frequently difficult to access for contemporary morphological and molecular analyses. We isolated and established five living strains of the rare fossil-taxon †Posoniella tricarinelloides from different sediment samples collected in the South China Sea, Yellow Sea and in the Mediterranean Sea (west coast off Italy). Here, we provide detailed descriptions of its morphology and conducted phylogenetic analyses based on hundreds of accessions and thousands of informative sites on concatenated rRNA datasets. Within the monophyletic Peridiniales, †P. tricarinelloides was reliably nested in the Thoracosphaeraceae and exhibited two distinct morphological types of coccoid cells. The two morphologies of coccoid cells would have been assigned to different taxa at the subfamily level if found separately in fossil samples. Our results thus challenge previous classification concepts within the dinophytes and underline the importance of comparative morphological and molecular studies to better understand the complex biology of unicellular organisms such as †P. tricarinelloides.

Homo floresiensis Contextualized: A Geometric Morphometric Comparative Analysis of Fossil and Pathological Human Samples

The origin of hominins found on the remote Indonesian island of Flores remains highly contentious. These specimens may represent a new hominin species, Homo floresiensis, descended from a local population of Homo erectus or from an earlier (pre-H. erectus) migration of a small-bodied and small-brained hominin out of Africa. Alternatively, some workers suggest that some or all of the specimens recovered from Liang Bua are pathological members of a small-bodied modern human population. Pathological conditions proposed to explain their documented anatomical features include microcephaly, myxoedematous endemic hypothyroidism (“cretinism”) and Laron syndrome (primary growth hormone insensitivity). This study evaluates evolutionary and pathological hypotheses through comparative analysis of cranial morphology. Geometric morphometric analyses of landmark data show that the sole Flores cranium (LB1) is clearly distinct from healthy modern humans and from those exhibiting hypothyroidism and Laron syndrome. Modern human microcephalic specimens converge, to some extent, on crania of extinct species of Homo. However in the features that distinguish these two groups, LB1 consistently groups with fossil hominins and is most similar to H. erectus. Our study provides further support for recognizing the Flores hominins as a distinct species, H. floresiensis, whose affinities lie with archaic Homo.

Pollen, phytoliths, arthropods and high-resolution 14C sampling from Rano Kau, Easter Island: evidence for late Quaternary environments, ant (Formicidae) distributions and human activity

A 20-m sediment core from Rano Kau, Easter Island provides plant microfossil, arthropod fossil and high-resolution 14C sampling evidence for late Quaternary environments, ant (Formicidae) distributions and human activity. The record commences prior to or during the LGM, providing the two oldest dates for Rano Kau thus far, 20,340 ± 160 and 34,260 ± 440 BP. The vegetation at the time was mostly Arecaceae (palm)-dominated grassland-woodland, suggesting cooler/drier conditions than present. Near the start of the Holocene c. 12,500 cal BP, climate alleviation is indicated by Asteraceae shrubland increasing at the expense of grassland. There is an early to mid-Holocene sediment hiatus. The record recommences c. 3,500 cal BP, with in-washed clayey soils bearing charcoal and phytoliths of Polynesian-introduced Musa (banana) and mixed with wetland detritus, reflecting slumping as a result of forest clearance and gardening. Dates of material of other plants from the clay/detritus layers containing the Musa phytoliths are older than expected, within the range 3,680–2,750 cal BP, well before settlement of Eastern Polynesia. This could reflect horticultural material settling in an older part of the sediment column or age increase by reservoir effects. A long section of overlying unconsolidated detritus provides a progressively younger upward sequence from 2,840–2,870 to 930–810 cal BP, but dates are variable after 1,290–1,180 cal BP, where charcoal, disturbance-related pollen and older than expected ant exoskeletons demonstrate the inclusion of pre-aged material from the floating vegetation mat or upslope erosion in the sediment column. Arecaceae pollen declines sharply after 930–810 cal BP. Arthropod analysis reveals two new weevil species for Easter Island, and two ant taxa, Tetramorium bicarinatum and Pheidole sp., the latter of which is also new for the island. Three of the four age determinations on ant remains suggest that ants were present prior to c. 2,500 cal BP, and probably associated with the period following the resumption of sediment accumulation c. 3,500 cal BP. Unless the apparent ant 14C ages have been increased by reservoir effects, these results question the long-accepted assumption that all Eastern Polynesia’s ant species have been introduced.

A deep dig––hindsight on Holocene vegetation composition from ancient environmental DNA

Want a glimpse at past vegetation? Studying pollen and other plant remains, which are preserved for example in lake sediments or mires for thousands of years, allows us to document regional occurrences of plant species over radiocarbon-dated time series. Such vegetation reconstructions derived from optical analyses of fossil samples are inherently incomplete because they only comprise taxa that contribute sufficient amounts of pollen, spores, macrofossil or other evidences. To complement optical analyses for paleoecological inference, molecular markers applied to ancient DNA (aDNA) may help in disclosing information hitherto inaccessible to biologists. Parducci et al. (2013) targeted aDNA from sediment cores of two lakes in the Scandes Mountains with generic primers in a meta-barcoding approach. When compared to palynological records from the same cores, respective taxon lists show remarkable differences in their compositions, but also in quantitative representation and in taxonomic resolution similar to a previous study (Jørgensen et al. 2012). While not free of assumptions that need critical and robust testing, notably the question of possible contamination, this study provides thrilling prospects to improve our knowledge about past vegetation composition, but also other organismic groups, stored as a biological treasure in the ground.

A CONOP9 composite taxon range chart for Ordovician conodonts from Baltoscandia: a framework for biostratigraphic correlation and maximum-likelihood biodiversity analyses

The Middle and Upper Ordovician rocks of Baltoscandia have been divided into spatially distinct, composite litho- and biofacies units called confacies belts. The precise regional correlation of outcrops and boreholes, which is necessary for biodiversity analyses, has always been problematic due to the pronounced biogeographical differentiation of macrofossils and horizontal lithofacies changes. We used a computer-assisted numerical sequencing program (CONOP9) to construct a correlation model and composite range chart from the stratigraphic range data of 159 conodont species in 24 boreholes and outcrops in Baltoscandia. We converted the composite section into a timescale in which to calculate the biodiversity, extinction, origination and fossil sampling probabilities through the Ordovician Period. Rates of extinction and origination were calculated using both simple approaches which do not incorporate estimates of sampling probability, and also more complex maximum-likelihood approaches based on Capture–Mark–Recapture (CMR) models. Our data show that overall biodiversity increases steadily from the base of the Paltodusdeltifer Zone to the uppermost Baltoniodusnorrlandicus Zone and then maintains an uneven diversity plateau until the earliest Sandbian. Diversity then declines dramatically throughout the remainder of the Ordovician. CMR analyses suggest that extinction rates remain constant throughout most of the Ordovician indicating that the dramatic late Middle and Late Ordovician decline in conodont diversity in Baltoscandia is attributable to depressed origination.

Mandibular shape analysis in fossil hominins: Fourier descriptors in norma lateralis

Biological shape can be defined as the boundary of a form in 2-space (ℝ2). An earlier study (Lestrel et al., 2010, HOMO-J. Comp. Hum. Biol.) of the cranial vault found that there were statistically significant differences between each of the three groups: H. erectus, H. heidelbergensis, and H. neanderthalensis compared with H. sapiens. In contrast, there was no statistically significant difference among the first three groups. These results suggest that these three groups may have formed single evolving lineage while H. sapiens represents a separate evolutionary development. The purpose of the current research was to discern if the mandible reflected a similar pattern as the cranial vault data. This study used lateral jpeg images of the mandible. Five fossil samples were used: A. robustus (n=7), H. erectus (n=12), H. heidelbergensis (n=4), H. neanderthalensis (n=22) and H. sapiens (n=61). Each mandible image was pre-processed with Photoshop Elements. Each image was then submitted to a specially written routine that digitized the 84 points along the mandible boundary. Each mandible was fitted with elliptical Fourier functions (EFFs). Procrustes superimposition was imposed to insure minimum shape differences. The mandible results largely mirrored the earlier cranial vault study with one exception. Statistically significant results were obtained for the mandible between the H. erectus and H. neanderthalensis samples in contrast to the earlier cranial vault data. F-tests disclosed that the statistical significance was limited to the anterior symphysis of the mandible. This mosaic pattern may be explained by the reduction in prognathism with the concomitant if rudimentary development of the chin as seen in H. neanderthalensis compared to H. erectus.

More Genomes From Denisova Cave Show Mixing of Early Human Groups

Analyses of three fossil samples using a powerful new method paint a complex picture of mingling among different ancient human groups.

PLEISTOCENE LANDSNAILS FROM THE COASTAL PLAIN OF ISRAEL

ABSTRACT The landsnail fauna of five fossil layers from two sites in Israel's Coastal Plain, Shefayyim and Netanya, was analyzed. The layers date from about 45,000 years ago to Subrecent. Four species were found: The Jaminia ovularis-sulcidens complex, Trochoidea (Xerocrassa) davidiana, Xeropicta vestalis and Monacha syriaca—all present in the Coastal Plain today. Remarkable is the absence from all fossil layers of Theba pisana, the landsnail most abundant today in the Coastal Plain. As T. pisana has also not been found in any other Pleistocene sites in Israel, it is suggested that this species was introduced into Israel during historic times. Thereafter, competition with Xeropicta vestalis was inevitable, as both occupy a similar habitat. Rich morph diversity occurs in recent Xeropicta as compared with the fossil samples; it is tempting to suggest that this outburst of diversity is related to some ecological displacement into which Xeropicta was perhaps forced, due to competition with Theba. Within Jamin...

Assessing the performance of a diatom transfer function on four Minnesota lake sediment cores: effects of training set size and sample age

Paleolimnological information is often extracted from diatom records using weighted averaging calibration and regression techniques. Larger calibration sample sets yield better inferences because they better characterize the environmental characteristics and species assemblages of the sample region. To optimize inferred information from fossil assemblages, however, it is worth knowing if fewer calibration samples can be used. Furthermore, confidence in environmental reconstructions is greater if we consider the relative importance of (A) similarity between fossil and calibration assemblages and (B) how well fossil taxa respond to the environmental variable of interest. We examine these issues using ~200-year sediment profiles from four Minnesota lakes and a 145-lake surface sediment training set calibrated for total phosphorus (TP). Training set sample sizes ranging from 10 to 145 were created through random sample selection, and models based on these training sets were used to calculate diatom-inferred (DI) TP data from fossil samples. Relationships between DI-TP variability and sample size were used to determine the minimum sample size needed to optimize the model for paleo-reconstruction. Similarly, similarities between fossil and modern assemblages were calculated for each size training set. Finally, fossil and modern assemblages were compared to determine whether older fossil samples had poorer similarity with modern analogs. More than 50–80 samples, depending on lake, were needed to stabilize variability in DI-TP results, and >110 training set samples were needed to minimize modern-fossil assemblage dissimilarities. Dissimilarities appeared to increase with sample age, but only one of the four studied cores displayed a significant trend. We have two recommendations for future studies: (1) be cautious when dealing with smaller training sets, especially if they are used to interpret older fossil assemblages and (2) understand how well fossil taxa are attuned to the variable of interest, as it is critical to evaluating the quality of the diatom-inferred data.

Pollen‐based palaeoclimate reconstructions over long glacial–interglacial timescales: methodological tests based on the Holocene and MIS 5d–c deposits at Sokli, northern Finland

AbstractPalaeoclimatic reconstructions over long glacial–interglacial timescales present major methodological challenges, as calibration methods based on modern climate and vegetation patterns may yield biased reconstructions from non‐analogue palaeoclimates, such as severely continental glacial periods. In this work, we present pollen‐based summer temperature reconstructions based on Holocene and early Weichselian (Marine Isotope Stages (MIS) 5d–c) deposits at Sokli, northern Finland. We attempt to improve the robustness of reconstructions of continental palaeoclimates by using two calibration sets, with one located in the region surrounding the fossil site and another in a more continental region in northwestern Russia. We assess the reliability of the reconstructions by estimating the compositional fit between fossil and calibration samples and by comparison with independent, plant macrofossil‐based reconstructions. We find the fossil samples to fit the extra‐regional, high‐continentality calibration set better during the early Holocene and MIS 5d–c. Especially in MIS 5d–c, however, even the high‐continentality calibration set fits the fossil samples poorly compared to the fit achieved in the mid and late Holocene. Thus our results highlight the problem of finding applicable calibration data for pre‐Holocene periods. We finally discuss the relative strengths and vulnerabilities of different calibration methods in reconstruction of non‐analogue palaeoclimates. Copyright © 2013 John Wiley & Sons, Ltd.

Taxonomic diversity, stratigraphic range, and exceptional preservation of Juro-Cretaceous salamanders from northern China

Since the late 1990s, eight localities in volcanic shale-rich lacustrine deposits of Middle Jurassic through Early Cretaceous age in northern China (western Liaoning Province, northern Hebei Province, and southern Inner Mongolia) have yielded thousands of exceptionally well-preserved salamander specimens. With 10 species published and several new taxa yet to be named and described, the fossil samples from northern China represent the most species-diverse, individually abundant, and exquisitely preserved salamander fossil assemblage known from the Mesozoic Era. The stratigraphic range of the fossil record covers a geologic time span of roughly 40–45 million years from the Middle Jurassic (Bathonian) through the Early Cretaceous (Aptian). In contrast to the well-known stem-group salamanders Karaurus and Kokartus from the Middle to Late Jurassic of Middle Asia, the Chinese record contains the earliest known crown-group salamanders, including Jurassic representatives of both Cryptobranchoidea and Salamandroidea. The Chinese Mesozoic record includes numerous examples of virtually complete larval, juvenile, young adult, and fully grown adult individuals that collectively provide key information on the life histories and developmental patterns of the earliest known crown-group salamanders. Many specimens show preservation of soft tissue structures, including body outline, eye, liver, and external gill filaments. This kind of soft tissue preservation is unusual for fossil salamanders, so the Chinese Mesozoic specimens are important for furnishing otherwise unavailable information on the life history, diversity, and ecological adaptations of early crown-group salamanders.

The occurrence of an abdominal fauna in an articulated tapir (Tapirus polkensis) from the Late Miocene Gray Fossil Site, northeastern Tennessee

The analysis of samples recovered from the abdominal area of an articulated tapir (Tapirus polkensis) from the Late Miocene (4.5-7 million BP) Gray Fossil Site (GFS) revealed a rich palyno-fauna comprised of about 94% egg/oocyst-like structures and 6% pollen and other palynomorphs. In addition, a group of 6 hickory nuts (Carya) was recovered from the same area suggesting that the samples represent the abdominal contents. The analysis of a sample from immediately outside the tapir produced a sample with 98% pollen and less than 0.5% egg/oocyst-like structures. The size, shape, and general morphology of egg/oocyst-like structures were analyzed with light and scanning electron microscopy and were compared to a variety of intestinal parasites found in extant ungulates, and the Perissodactyla in particular. We also compared fossil structures to the numbers and kind of intestinal parasites recovered from fecal samples from the Baird's tapir (T. bairdii) in Costa Rica and from samples collected from the lowland tapir (T. terrestris) from Ecuador to assess their similarity to our fossil sample. Based on these data, we discuss what role parasites may have played in the biology of T. polkensis during the Late Miocene-Early Pliocene.

Impoverishment of recent floodplain forest mollusc fauna in the lower Ohře River (Czech Republic) as a result of prehistoric human impact

Analyses of fossil mollusc successions have rarely been used to study the development of floodplain forests during the Holocene. The Ohře River, located in a prehistorically settled chernozem area in the Czech Republic, is partly situated in Cretaceous marlstones, yielding sediments suitable for fossilization directly in floodplain deposits. We analysed five fossil mollusc successions situated in the lower stretch of the Ohře River and compared the results with recent mollusc assemblages studied along the entire 256 km of the river. Fossil samples were composed mostly of open‐country species throughout the Holocene or the whole preserved succession. Only some samples also contained woodland assemblages, but these were always greatly impoverished, with a very low frequency of strictly woodland species. Although the natural‐looking appearance of the present‐day floodplain forests of the lower river stretch has resulted in its being declared a nature reserve, modern floodplain forest mollusc assemblages there are also impoverished. This reduction in the distribution of strictly woodland species compared with modern assemblages in the upper stretch of the river seems to be the result of an ancient human settlement and continuous disturbances of the floodplain forest development since the Neolithic. Thus, fully developed floodplain forest assemblages occur recently only in the upper non‐impacted stretch of the river. Based on the studied fossil successions we can conclude that the lower Ohře River floodplain was probably a mosaic of open and disturbed forest habitats throughout the Holocene. This area is part of a central European landscape island, where forests probably never fully developed and open patches from the early Holocene continually developed into an agricultural landscape.

What constitutes a fossil mammal community in the early Miocene Santa Cruz Formation?

ABSTRACT The late early Miocene Santa Cruz Formation of southern Argentina is one of the most productive fossil mammal-bearing formations in South America. Hundreds of species have been named from this formation, resulting in a clear overestimation of Santa Cruz mammal diversity. Recent paleoecological studies have attempted to minimize this problem by analyzing more restricted faunal lists based only on recently collected fossil samples. In some cases, this approach has excluded clearly recognizable Santa Cruz taxa. In this study, I use a family-level rarefaction analysis of the Yale Peabody Collection of Santa Cruz fossils to test whether such absences are likely due to smaller sample size or to other factors. I further attempt to reconcile these absences using distributional data from recent and historical Santa Cruz Formation collections. The rarefaction analysis indicates that most single-locality samples from the Santa Cruz Formation have lower familial diversity than expected based on the taxonomic distribution of specimens in the Princeton University Collection at Yale Peabody Museum (YPM-PU) as a whole. However, I conclude that single-locality samples are not necessarily more appropriate than multi-locality samples for paleoecological analyses of ancient Santa Cruz Formation mammal communities given the large regional extent of the Santa Cruz Formation and the large geographic area encompassed by most modern communities used for comparison. This study highlights the need for more precise stratigraphic correlations among Santa Cruz localities and integration of important historical collections into a modern stratigraphic framework.