Recent results indicate that charm quarks are intrinsic components of the proton wave function, and that the charm and anticharm distributions for a given value of the Bjorken-x variable can be different. In this paper, we will investigate the impact of this asymmetric intrinsic charm on the production of D0 and D¯0 mesons for fixed target p+Ne20 collisions at the LHCb. In our calculations, we include the contribution of the gluon-gluon fusion, gluon-charm and recombination processes and assume distinct models for the treatment of the intrinsic charm component. We demonstrate that the presence of an intrinsic charm improves the description of the current data for the rapidity and transverse momentum distributions of D mesons. However, such models are not able to describe the LHCb data for the D0−D¯0 asymmetry at large transverse momentum, which point out that the description of the intrinsic charm needs to be improved and/or new effects should be taken into account in the production of heavy mesons at forward rapidities in fixed-target collisions. Published by the American Physical Society 2024