Infant Formula Milk Research Articles

Storage Duration and Added Docosahexaenoic Acid Modify the Rates of Esterified and Free Oxylipin Formation in Infant Milk Formula

Infant milk formulas often contain docosahexaenoic acid (DHA), a highly unsaturated fatty acid that is prone to oxidation. Previously, we reported in oil that the esterified lipid pool is more prone to lipid oxidation than the free pool. However, it is unknown whether added DHA modifies lipid oxidation in infant formula. In the present study, we quantified lipid oxidation rates in infant milk formula containing canola oil (F1) or canola oil supplemented with DHA-ethyl ester (F2). Lipid oxidation kinetics were determined by quantifying esterified and free oxylipins using liquid chromatography-tandem mass spectrometry (LC-MS/MS) during storage for 21 days at 4 °C. Esterified oxylipins increased in concentration within 3 and 7 days of storage in F2 (with DHA) and F1, respectively. Free oxylipins appeared 7 and 14 days later in F2 and F1, respectively. The kinetic estimates revealed that esterified oxylipins formed at a faster rate in both formulas compared to free oxylipins. Surprisingly, in F2 (which contains DHA), the rates of formation of both esterified and free linoleic acid and alpha-linolenic acid-derived oxylipins were higher than in F1. This study demonstrated that in food systems, DHA promotes the oxidation of other PUFAs, and that triacylglycerol/esterified lipids are preferentially oxidized over free fatty acids, highlighting the role of triacylglycerols in lipid oxidation.

A Study on the Analysis of Visual Elements of Infant Formula Milk Powder Brand Logo -Focus on the Top 10 Brands in the Chinese Market-

A Study on the Analysis of Visual Elements of Infant Formula Milk Powder Brand Logo -Focus on the Top 10 Brands in the Chinese Market-

Market research of infant formula milks in Spain.

There are circumstances in which breastfeeding is not possible and formula milk feeding has to be resorted to. In these cases, it is difficult to choose among the multitude of brands on the market for infant feeding. Although the composition of formula milk is largely regulated by legislation, there are certain nutrients whose presence in breast milk is beneficial. This circumstance will help us to establish some criteria to choose formula milk for a healthy infant. Among all the formula milks which can be found in different shops (pharmacies, supermarkets, etc.), market research has been carried out based on the nutritional criteria: the lipid and protein profile, certain carbohydrates, amino acids, and vitamins, as well as the presence of nucleotides, prebiotics, and probiotics. Based on these results, it can be established which formula milk we would give to a healthy infant. Thus, within the analyzed formula milks, it has been concluded that the most complete milk in this would be formula milk D and the least formula milk J. Although more studies are needed to confirm this, it is foreseeable that, as the scientific evidence is greater, the legislation will be updated and considering these nutrients for a correct formula milk.

Rapid detection of micronutrient components in infant formula milk powder using near-infrared spectroscopy.

In order to achieve rapid detection of galactooligosaccharides (GOS), fructooligosaccharides (FOS), calcium (Ca), and vitamin C (Vc), four micronutrient components in infant formula milk powder, this study employed four methods, namely Standard Normal Variate (SNV), Multiplicative Scatter Correction (MSC), Normalization (Nor), and Savitzky-Golay Smoothing (SG), to preprocess the acquired original spectra of the milk powder. Then, the Competitive Adaptive Reweighted Sampling (CARS) algorithm and Random Frog (RF) algorithm were used to extract representative characteristic wavelengths. Furthermore, Partial Least Squares Regression (PLSR) and Support Vector Regression (SVR) models were established to predict the contents of GOS, FOS, Ca, and Vc in infant formula milk powder. The results indicated that after SNV preprocessing, the original spectra of GOS and FOS could effectively extract feature wavelengths using the CARS algorithm, leading to favorable predictive results through the CARS-SVR model. Similarly, after MSC preprocessing, the original spectra of Ca and Vc could efficiently extract feature wavelengths using the CARS algorithm, resulting in optimal predictive outcomes via the CARS-SVR model. This study provides insights for the realization of online nutritional component detection and optimization control in the production process of infant formula.

Peptidome comparison on the immune regulation effects of different casein fractions in a cyclophosphamide mouse model

The protein composition of human milk plays a crucial role in infant formula milk powder formulation. Notably, significant differences exist between bovine casein and human milk casein. Previous studies have shown that casein hydrolysates could enhance immune function; however, gastrointestinal dyspepsia in infants affects the type and function of peptides. Therefore, the present study used peptidomics to sequence and analyze hydrolyzed peptides from different casein fractions. Additionally, animal experiments were conducted to assess the functionality of these casein fractions and elucidate their differences. The results revealed variations in peptide composition among the different casein fractions of formula milk powder. Interestingly, milk powder formulated with both β- and κ-casein (BK) exhibited significant enrichment of peptides related to the immune system. Moreover, the BK group significantly alleviated immune organ damage in cyclophosphamide-treated mice and regulated serum levels of pro-inflammatory and anti-inflammatory factors. Furthermore, feeding different casein fractions influenced the intestinal microflora of cyclophosphamide-treated mice, with the BK group mitigating the changes caused by cyclophosphamide. In conclusion, the findings suggest that BK formula in milk powder has the potential to positively enhance immunity. This study provides a robust theoretical basis for human-emulsified formula milk powder development.

Human Milk, Infant Formula, and Other Milks Fed to Infants and Toddlers in the United States, NHANES 2007-2018.

For the first time, the 2020-2025 Dietary Guidelines for Americans provide specific guidance regarding the types of foods and beverages that should be offered in the first 2 years of life. Milk, in various forms (eg, human milk, infant formula, and cow's milk) contributes a large proportion of key nutrients to the diets of infants and toddlers in the United States. The aim of this study was to determine the types of milk (human milk, infant formula, and other milk) fed to US infants and toddlers in the past 12 years and to describe trends over time. This was a cross-sectional analysis of 2-day, 24-hour dietary recalls. Data from the 2007-2018 National Health and Nutrition Examination Survey were used for these analyses. Infants and toddlers aged 0 through 23.9 months with 2 days of dietary recall data (n= 3,079) were included. The main outcome was proportion of infants and toddlers fed different milk types. Survey-adjusted weighted percentages were used to report sociodemographic characteristics and the proportion of subjects fed each milk type category by age group and survey cycles. Binary and multinomial logistic regressions were used to assess differences in subject characteristics by age groups. Sociodemographic characteristics did not differ by age group. The proportion of infants aged 0 to <6 months fed infant formula only was 60.2% in 2007-2012 and 44.8% in 2013-2018. The proportion of infants aged 6 to <12 months fed partially hydrolyzed infant formula only was 7.3% in 2007-2012 and 13.1% in 2013-2018. In toddlers (>12 months old), cow's milk was the predominant milk type in both 2007-2012 and 2013-2018. The percentage of infants fed any human milk increased over the past decade. Unsweetened cow's milk was the most predominate milk type consumed among toddlers.

Defining the lipid profiles of human milk, infant formula, and animal milk: implications for infant feeding.

Breastfed infants have lower disease risk compared to formula-fed infants, however, the mechanisms behind this protection are unknown. Human milk has a complex lipidome which may have many critical roles in health and disease risk. However, human milk lipidomics is challenging, and research is still required to fully understand the lipidome and to interpret and translate findings. This study aimed to address key human milk lipidome knowledge gaps and discuss possible implications for early life health. Human milk samples from two birth cohorts, the Barwon Infant Study (n = 312) and University of Western Australia birth cohort (n = 342), were analysed using four liquid chromatography-mass spectrometry (LC-MS) methods (lipidome, triacylglycerol, total fatty acid, alkylglycerol). Bovine, goat, and soy-based infant formula, and bovine and goat milk were analysed for comparison. Composition was explored as concentrations, relative abundance, and infant lipid intake. Statistical analyses included principal component analysis, mixed effects modelling, and correlation, with false discovery rate correction, to explore human milk lipidome longitudinal trends and inter and intra-individual variation, differences between sample types, lipid intakes, and correlations between infant plasma and human milk lipids. Lipidomics analysis identified 979 lipids. The human milk lipidome was distinct from that of infant formula and animal milk. Ether lipids were of particular interest, as they were significantly higher, in concentration and relative abundance, in human milk than in formula and animal milk, if present in the latter samples at all. Many ether lipids were highest in colostrum, and some changed significantly through lactation. Significant correlations were identified between human milk and infant circulating lipids (40% of which were ether lipids), and specific ether lipid intake by exclusively breastfed infants was 200-fold higher than that of an exclusively formula-fed infant. There are marked differences between the lipidomes of human milk, infant formula, and animal milk, with notable distinctions between ether lipids that are reflected in the infant plasma lipidome. These findings have potential implications for early life health, and may reveal why breast and formula-fed infants are not afforded the same protections. Comprehensive lipidomics studies with outcomes are required to understand the impacts on infant health and tailor translation.

Nano- and Microplastics Migration from Plastic Food Packaging into Dairy Products: Impact on Nutrient Digestion, Absorption, and Metabolism.

The ongoing use of plastic polymers to manufacture food packaging has raised concerns about the presence of nano- and microplastics (NMPs) in a variety of foods. This review provides the most recent data on NMPs' migration from plastic packaging into dairy products. Also discussed are the possible effects of NMPs on nutrient digestion, absorption, and metabolism. Different kinds of dairy products, including skimmed milk, whole liquid milk, powder milk, and infant formula milk, have been found to contain NMPs of various sizes, shapes, and concentrations. NMPs may interact with proteins, carbohydrates, and fats and have a detrimental impact on how well these nutrients are digested and absorbed by the body. The presence of NMPs in the gastrointestinal tract may impact how lipids, proteins, glucose, iron, and energy are metabolized, increasing the risk of developing various health conditions. In addition to NMPs, plastic oligomers released from food packaging material have been found to migrate to various foods and food simulants, though information regarding their effect on human health is limited. Viewpoints on potential directions for future studies on NMPs and their impact on nutrient digestion, absorption, and health are also presented in this review.

Sweetness Assessment of an Infant Formula Milk Powder with Added Sucralose

Sweetness Assessment of an Infant Formula Milk Powder with Added Sucralose

Omics Analysis of Extracellular Vesicles Recovered from Infant Formula Products and Milk: Towards Personalized Infant Nutrition.

Milk and milk products such as infant formula (IF) play a fundamental role in serving the nutritional needs of the developing infant. Extracellular vesicles (EVs) in human (HM) and cow milk (CM) contain molecular cargo such as proteins and micro(mi)RNAs that serve as functional messengers between cells and may be of importance to infant health. Most IF is derived from a CM protein base, however differences between HM and CM EV molecular cargo have not been extensively studied. This study develops a pipeline using advanced proteomics and transcriptomics to enable cross-species comparison of milk and IF EVs. The number of nanoparticles per mL of IF is significantly reduced compared to unprocessed CM. 130 proteins and 514 miRNAs are differentially abundant between HM and CM EVs. While 90% of CM EV miRNAs are also identified in IF EVs, only 20% of CM EV proteins are identified in IF EVs. This workflow identifies key species-specific differences that can be used to optimize IF recipes and enhance infant nutrition. Improved preservation of EV functional molecular cargo in IF products is of critical importance to retaining molecular drivers of good health and should be the focus of future investigations.

Safety and quality evaluations of liquid milk and infant formula products in China in 2022

AbstractMilk serves two nutritional functions: it provides nutrition while also maintaining a healthy intestinal microenvironment. Thus, the safety and quality of milk products are critical for consumers' health. To evaluate liquid milk and infant formula products in China, we analyzed nine indices of 294 batches of pasteurized milk (PM), 92 batches of ultra‐high temperature milk (UHT), and 20 batches of infant formula milk powder (IF) collected from 21 provinces in 2022. All PM, UHT, and IF samples were satisfactory concerning aflatoxin M1 contamination, contamination by heavy metals, and residues of veterinary drugs and pesticides. The contents of lactoferrin, α‐lactalbumin, β‐lactoglobulin, furosine, and lactulose in the majority of samples met the group standards. Moreover, the contents of lactoferrin, α‐lactalbumin, and β‐lactoglobulin were higher in PM than in UHT, while furosine and lactulose contents showed the opposite trend. The findings concerning the safety and quality of milk products in China provide important insights to aid consumer preferences for milk products.

Effects of radio frequency heating on the glass transition, protein structure, and volatile compounds profile of commercial powdered infant formula milk

Radio frequency (RF) has found its potential in ensuring the safety of packaged powdered infant formula milk (PIFM). PIFM was processed by RF at different temperature-holding time treatments (70 °C-115 min, 75 °C-50 min, 80 °C-25 min, 85 °C-10 min, and 90 °C-5 min) to obtain 5-log reduction of Cronobacter sakazakii, and its physicochemical characteristics were investigated. Various RF treatments possessed insignificant effects on color difference (ΔE < 1.5), solubility (>99%), crystallinity (∼5%), surface chemical composition (>30% fat, 15%–20% protein, and 45%–50% lactose), and microstructure of PIFM. Lower temperature-longer holding time treatment as 70 °C-115 min caused 51% reduction in water activity and 71% reduction in moisture content, thereby considerably increasing the glass transition temperature of PIFM from 60.3 °C to 78.4 °C. Protein secondary structures were preserved; however, 75 °C-50 min and 80 °C-25 min treatments significantly (P < 0.05) decreased the α-helix structure. All RF heating conditions significantly (P < 0.05) altered the concentration of volatile organic compounds in PIFM and increased the relative abundance of lipid oxidation substances such as (E)-2-heptenal, 2-heptanone, and n-nonanal. PIFM can be treated at 90 °C for 5 min for better quality and processing efficiency.

Dried Baby Formula as a Source of Opportunistic Members of Enterobacteriaceae for Infants

For evaluating the prevalence of Enterobacteriaceae in baby formulas, we examined 60 different samples of powdered infant milk formula and milk-cereal based infant formula that is recommended for first-day birth and from 6-month age, respectively, and collected from various supermarkets, shops, and pharmacies. Six (20.0%) and thirteen (43.3%) powdered infant milk formulas and milk-cereal based infant formulas, respectively, were contaminated by different Enterobacteriaceae involved Enterobacter cloacae ssp. cloacae, Pantoea spp., Enterobacter hormaechei, and Raoultella ornithinolytica, which were identified using traditional biochemical tests and the vitek2 compact system. Only five samples of investigating milk-cereal based infant formula type have coliforms count of 4 MPN/g. All species were susceptible to gentamycin, while all were resistant to cephalothin antibiotics. Eventually, 20.0 % of powdered infant milk formula samples were unacceptable referred to Egyptian standards (2072/2006) and most of the comparable international standards. In the study, we proved the contamination of powdered milk baby formula by Enterobacteriaceae, which mainly causes health problems in immature and immunocompromised infants. The dried infant formula factories are more needful for good hygiene and safety systems applications. This study is one of the fewest studies conducted in Egypt to isolate Enterobacteriaceae members from dried foods; especially milk-cereal based infant formula.

Comparative Evaluation of Maintenance of Cell Viability of an Experimental Transport Media "Ringer's Lactate" with Dextrose Normal Saline ORS Egg White and Infant Milk Formula for Transportation of an Avulsed Tooth.

The viability of the periodontal ligament (PDL) cells on the root surface of the avulsed tooth determines the prognosis of the replanted tooth, which in turn is determined by a suitable transport medium in which the tooth was stored. The aim of the present study is to evaluate and compare the effectiveness of Ringer's lactate (RL) as a storage medium for an avulsed tooth in maintaining the PDL cell viability with dextrose normal saline (DNS), oral rehydration salt (ORS), egg white (EW), and infant milk formula (IMF). A total of 85 freshly extracted human teeth were divided into five experimental groups and two control groups. The positive and negative controls corresponded to 0-minute and 8-hour dry time, respectively. The experimental teeth were stored dry for 30 minutes and then immersed in one of five experimental media (RL, DNS, ORS, EW, and IMF) for 45 minutes. The teeth were then treated with collagenase type III and trypsin for 10 minutes. The number of viable PDL cells was counted with a hemocytometer and analyzed. Statistical analysis showed that IMF, RL, and EW had no statistically significant differences among them in maintaining the viability of the PDL cells but were significantly better than DNS. No statistically significant difference between RL, EW, and ORS in the number of viable PDL cells. Infant milk formula (IMF), RL, and EW showed similar results within the parameters of the study; they can be used as alternative storage media for avulsed teeth. DNS showed poor results, and ORS could serve as short-term storage media if the other solutions are not readily available. The search for an appropriate storage media with favorable pH and osmolality along with easy availability is the basic thought behind this study. Kumar P, Kotumachagi SS, Fabi AJ, et al. Comparative Evaluation of Maintenance of Cell Viability of an Experimental Transport Media "Ringer's Lactate" with Dextrose Normal Saline ORS Egg White and Infant Milk Formula for Transportation of an Avulsed Tooth. Int J Clin Pediatr Dent 2023;16(3):453-458.

Application of a novel phage LPCS28 for biological control of Cronobacter sakazakii in milk and reconstituted powdered infant formula

Contamination of infant formula with Cronobacter sakazakii (C. sakazakii) can cause fatal infections in neonates. Phages have emerged as promising antibacterial agents for food safety, but their effectiveness may be limited by thermal processing. In this study, we isolated 27 C. sakazakii phages from environmental water samples and selected LPCS28 due to its broad lysis spectrum. The titer of LPCS28 will not be significantly affected by heating at a temperature of 60 °C for one hour. In both reconstituted powdered infant formula (RPIF) and liquid milk, the pre-added LPCS28, after the thermal processing at 63 °C for 30 min, significantly inhibited the post-contaminated C. sakazakii (103 CFU/mL) and eventually reduced the number of C. sakazakii to below the limit of detection (<10 CFU/mL) within 9 h at 37 °C and significantly delayed the increase of bacterial concentration in the samples at 23 °C. The phylogenetic analysis revealed that LPCS28 belonged to a new genus, we proposed as Nanhuvirus, under the family Straboviridae. These findings suggest that phage LPCS28 is a promising biological control agent for pathogenic C. sakazakii in the dairy industry.

Development of Risk Management Mitigation Plans for the Infant Formula Milk Supply Chain Using an AHP Model

Infant formula milk (IFM) is critical in the diet of many babies and must be of high-quality. Unfortunately, IFM has been a target of adulteration by those attempting to make illegal profits and has suffered from contamination-related issues. This study’s main objective was to identify the most critical risks affecting IFM quality in the supply chain and determine mitigation strategies to improve IFM performance measurement. We developed a model to reduce adulteration and contamination rates in the infant formula milk supply chains (IFMSCs) and maximize safety. The steps to achieve the study’s objectives included: (1) identifying the importance of IFMs for infant nutrition and their risks; (2) establishing mitigation criteria for evaluating IFMSC’s performance to maximize quality; and (3) analyzing each mitigation criterion to maximize IFM safety. Based on pairwise comparisons by professionals in the food supply chain (FSC) of decision-making, the Analytic Hierarchy Process (AHP) model was used to analyze and prioritize mitigation alternatives. According to the contamination quality risk agent, mitigation alternative (QR.M2) ranked highest. This study’s findings illustrate how vital avoiding risk is when dealing with public health, especially infants’ health, and how IFM must undergo precise testing and quality checks at every supply chain stage to ensure quality.

English

Camel milk has numerous nutraceutical and therapeutical properties due to camel s desert habitat and grazing habits on wild vegetation. However limited literature was available about the milk composition of camel breeds of south Punjab in Pakistan. The current study aims to depict variations in nutritional aspects of milk among camel breeds of South Punjab Pakistan. The various parameters of milk i.e. physicochemical analysis fatty acids composition protein contents amino acid profiling and mineral concentrations were evaluated and characterized to provide fundamental knowledge to researchers interested in working in its medicinal role. Minerals i.e. Na K Zn and Pb in camel milk were found in considerable concentrations such as 405.94?0.02 204.16?0.10 2.45? 0.02 and 0.25?0.02 ppm accordingly. During physicochemical analysis pH and protein were present in higher amounts. Casein of camel breeds contains higher amounts of amino acids i.e. Leucine (0.25?0.039 %) Lysine (0.12?0.019 %) Aspartic acid (0.18?0.017 %) and Glutamic acid (0.56?0.087 %). Fatty acids were present in significant concentrations in all camel breeds i.e. Butyric acid (0.760?0.012 g/100g) Lauric acid (0.770?0.120 g/100g) Myristic acid (12.420?1.163 g/100g) and Linoleic acid (2.390?0.112 g/100g). Camel milk is less prone to allergies and can be used in dairy-based food products infant milk formulas and medicinal industries.

Simultaneous Determination of Neonicotinoid Insecticides and Metabolites Residues in Milk and Infant Formula Milk Powder by EMR-Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of Neonicotinoid Insecticides and Metabolites Residues in Milk and Infant Formula Milk Powder by EMR-Liquid Chromatography-Tandem Mass Spectrometry

Infant burn injuries related to water heating for powdered infant formula preparation.

Guidance for preparing powdered infant formula (PIF) helps to ensure it meets the nutritional needs of infants and is safe to consume. Among safety concerns is Cronobacter sakazakii contamination which can lead to serious infections and death. PIF preparation guidance varies; there is a lack of consensus on whether there is a need to boil water to inactivate potential Cronobacter and for how long to let the water cool before reconstitution. We sought to quantify the burden of burn injuries among infants related to water heating for PIF preparation. Estimating this burden may help inform preparation recommendations. Burn injuries among infants <18 months of age were identified from 2017 to 2019 National Electronic Injury Surveillance System data collected from sampled hospital emergency departments. Injuries were classified as related to PIF water heating, potentially related to PIF water heating but with undetermined causation, related to other infant feeding aspects, or unrelated to infant formula or breast milk feeding. Unweighted case counts for each injury classification were determined. Across sampled emergency departments, 7 PIF water heating injuries were seen among the 44,395 injuries reported for infants <18 months. No reported PIF water heating injuries were fatal, but 3 required hospitalization. Another 238 injuries potentially related to PIF water heating but with undetermined causation were also seen. Preparation guidance should consider both the potential risk for Cronobacter infection and the potential risk for burns.

Investigation on the addition of microbial food cultures in infant and follow-up formula in China from 2017 to 2022

To understand the addition of microbial food cultures in infant and follow-up formula milk powder in China. The product information of infant and follow-up formula milk powder approved in China from 2017 to 2022 was investigated, including the query platform and packaging label information, and the strains, addition rates and addition amount of microbial food cultures were statistically analyzed. From 2017 to 2022, a total of 1438 infant and follow-up formula milk powder products were approved in China, of which 434 products were added with microbial food cultures, 6 types of strains were used, namely Bifidobacterium animalis Bb-12, Bifidobacterium lactis HN019, Bifidobacterium lactis Bi-07, Lactobacillus fermentum CECT5716, Lactobacillus rhamnosus HN001 and Lactobacillus acidophilus NCFM, the top three addition rates were Bifidobacterium animalis Bb-12, Bifidobacterium lactis HN019 and Bifidobacterium lactis Bi-07, the addition rate were 79.72%, 18.43% and 12.67%, respectively. The addition amount of the strains ranged from 1×10~6 to 6×10~7 CFU/g, the median value was 1×10~6 CFU/g. There is insufficient scientific evidence on the feeding effect, types and amounts of microbial food cultures added to infant and follow-up formula in China.