Formation Of First Peptide Bond Research Articles

New evidence for the inhibition by harringtonine in the formation of the first peptide bond in protein synthesis.

New evidence for the inhibition by harringtonine in the formation of the first peptide bond in protein synthesis was obtained by means of experiments in the cell-free system. The antitumour drug strongly blocked dipeptide synthesis in the system without elongation factor G and GTP. Furthermore, it inhibited N-Acetyl-phenylalanyl-puromycin formation from N-Acetyl-phenylalanyl-tRNA, puromycin, and ribosomes.

Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond.

A simplified DNA-directed in vitro system has been developed to study the regulation of the synthesis of ribosomal protein L10 by measuring the formation of the first dipeptide, fMet-Ala. The results show that the inhibition of L10 synthesis by L10 (autoregulation) occurs at or prior to the formation of the first peptide bond.