Abstract Channel catfish Icialurus punctatus ranging from 70 to 148 mm in total length were treated with formalin at a concentration of 25 μL/L for 4 h daily on four successive days each week over a 28-week period beginning in mid-September. Coefficients of condition (weight-tolength ratios) and histological examinations of gill tissues were used to evaluate health of fish exposed to formalin. Gill tissues of both formalin-treated and untreated fish showed effects typical of intensive culture, including hypertrophy, hyperplasia, and excess mucous secretion; no differences were found in gill morphometry between the two groups. Channel catfish periodically treated with formalin had a significantly higher coefficient of condition (P < 0.05) after 28 weeks and no indication of adverse effects attributable to formalin.