Simple SummaryThe welfare of zoo birds kept under flight restraint is a frequently discussed topic. Therefore, this study was conducted with one of the most regularly kept types of deflighted birds in German zoos, the great white pelican, to find scientific data regarding welfare assessments of deflighted birds. The detection of corticosterone in feathers (CORTf) as a stress indicator for birds is an almost completely noninvasive form of measurement meant to evaluate the effects of deflighting birds in zoos. Three groups of animals were compared: irreversibly deflighted pelicans that were pinioned or extirpated, reversibly deflighted individuals that were feather-clipped, and airworthy pelicans that were able to fly. Combining two independent research methods, behavioral observation and the measurement of CORTf levels of great white pelicans, we aimed to obtain an objective overview of whether deflighted birds showed differences in CORTf or behavior compared to airworthy birds. As a result of the analysis, we found no significant differences in CORTf between flight-restricted and airworthy birds. However, reversibly deflighted pelicans had higher CORTf values than irreversibly deflighted and airworthy pelicans. In addition, pelicans living in groups consisting of more than 10 individuals showed lower CORTf values than pelicans in groups of less than 10 individuals. “Fluttering” behavior was significantly associated with higher CORTf values. In conclusion, the flight restriction of great white pelicans does not seem to impact the welfare indicators assessed in this study, adrenal activity, or behavior. The data show that the living conditions of pelicans (such as group size) may influence the welfare of these birds. To confirm this, further studies on other ground- and water-based birds are needed to provide more scientific data on animal welfare and living conditions in zoos.The pinioning of birds was previously one of the most-accepted forms of mutilation in zoos. Despite a lack of knowledge on the effects of deflighting procedures with regard to the well-being of deflighted birds, pelicans are often reversibly deflighted by feather-clipping to keep them in open enclosures, including those with ponds without netting. In the present study, we focused on the welfare implications of flight restraint on one of the most commonly kept types of birds in German zoos, the great white pelican. A combination of behavioral observations and feather corticosterone concentrations (CORTf) of pelicans with different deflighting statuses (i.e., irreversibly deflighted, reversibly deflighted, and airworthy) was used to evaluate the effects of deflighting status on pelican welfare. We observed 215 individuals in 21 different German zoos. The pelicans lived in differently designed exhibits. An ethogram for these species was developed and their behavior was evaluated by scan sampling. Feather samples from 182 individuals were collected to determine if different deflighting conditions influenced the CORTf and therefore stress levels. The hypothesis was that the CORTf values of airworthy pelicans differ from those of deflighted pelicans. Tendencies with regard to the flight status groups were found. Conversely, reversibly deflighted pelicans had higher CORTf levels than irreversible deflighted and airworthy pelicans. Tendencies with regard to CORTf values and the group size of the kept pelicans were observed. The CORTf values were lower in groups consisting of more than 10 animals. In addition, the frequency of fluttering behavior was positively associated with CORTf values. Pelicans that frequently showed fluttering had higher CORTf values. Therefore, fluttering behavior might be considered a sign of stress levels in pelicans. This study is one of the first important steps in assessing the impact of deflighting procedures on the welfare of great white pelicans kept in zoos.