Life Forms Research Articles

Bacterial Organelles in Iron Physiology.

Bacteria were once thought to be simple organisms, lacking the membrane-bound organelles found in eukaryotic cells. However, recent advancements in microscopy have changed this view, revealing a diverse array of organelles within bacterial cells. These organelles, surrounded by lipid bilayers, protein-lipid monolayers, or proteinaceous shells, play crucial roles in facilitating biochemical reactions and protecting cells from harmful byproducts. Unlike eukaryotic organelles, which are universally present, bacterial organelles are species-specific and induced only under certain conditions. This review focuses on the bacterial organelles that contain iron, an essential micronutrient for all life forms but potentially toxic when present in excess. To date, three types of iron-related bacterial organelles have been identified: two membrane-bound organelles, magnetosomes and ferrosomes, and one protein-enclosed organelle, the encapsulated ferritin-like proteins. This article provides an updated overview of the genetics, biogenesis, and physiological functions of these organelles. Furthermore, we discuss how bacteria utilize these specialized structures to adapt, grow, and survive under various environmental conditions.

Noncanonical Amino Acid Incorporation in Animals and Animal Cells.

Noncanonical amino acids (ncAAs) are synthetic building blocks that, when incorporated into proteins, confer novel functions and enable precise control over biological processes. These small yet powerful tools offer unprecedented opportunities to investigate and manipulate various complex life forms. In particular, ncAA incorporation technology has garnered significant attention in the study of animals and their constituent cells, which serve as invaluable model organisms for gaining insights into human physiology, genetics, and diseases. This review will provide a comprehensive discussion on the applications of ncAA incorporation technology in animals and animal cells, covering past achievements, current developments, and future perspectives.

Discrepancy in coordination and variation of root and leaf traits among herbaceous and shrub species in the desert, China

IntroductionAlterations in life forms could simultaneously influence the variability of fine root and leaf traits. However, our understanding of the organ-level coordination and patterns of variation in fine root and leaf traits among desert herbs and shrubs with distinct habits remains limited.MethodsConsequently, this study examined the leaf and fine root traits of 9 shrubs and 9 herbs across three desert habitats through Sperman's correlation analysis, principal component analysis (PCA), and standardized major axis (SMA) analysis as a means of identifying the hub traits and the resource trade-off strategies employed by desert plants to adapt to their environment.ResultsThe results showed that the extent of coordination between leaf and root traits, defined as similarity, was contingent upon the life form. While the traits in shrubs were completely decoupled, those in herbs exhibited a high degree of coordination. The traits related to water acquisition and storage are highly connected and do not depend on traits and life forms. Most leaves and fine roots were primarily loaded along the PC1 and PC2 axes of principal component analysis.DiscussionSuggesting that herbs and shrubs each adopt the most advantageous trait syndrome in accordance with their life form to acquire and conserve resources. The leaf economic spectrum and the root economic spectrum evolved independently, showing no dependence on the variations in life form. In conclusion, in desert plants, leaf and root variations occur independently along two axes, with traits associated with water acquisition playing a neutral role in their ecological strategy.

Looking Upstream:

“Looking upstream” refers to going beyond the negative effects of the current problems which disproportionately impacts historically marginalized families— water contamination, flooding, and scarcity—to see causation: why is the problem happening in the first place? In this article, we “look upstream” at our language and literacy practices and attempt to see how these are entangled with representations of our place in the water cycle. We examine a few curricular artifacts from our Literacy Clinic—dually focused on responsive literacy instruction and water justice—to illustrate our entanglements with linguistic systems, discourses, and narratives. Part of the way forward, we argue, is changing the ways we speak, write, and read in relation to our shared environment, including water and all forms of life.

Influence of Farmer’s Income Level on Adoption of Agricultural Water Management Practices among Smallholder Farmers in Rongai Sub-county, Kenya

Water is a resource that cannot be replaced and can only be renewed if it is well managed. It is basic for all forms of life, for every aspects of socio-economic development, and for the maintenance of a healthy ecosystem. Agriculture is the biggest water user globally, accounting for 70 percent of total water withdrawals on average. There are different practices that can aid in managing water used for agriculture. Some of these agricultural water management practices are; rainwater harvesting, irrigation, organic farming, and use of drought resistant crops. Farmer’s decision to either adopt or reject these practices can be affected by several factors, some of which are socio-economic. Some of the socio-economic factors that may influence adoption of these practices include farmer’s educational level, income level and farm size. This study examined the influence of farmer’s income level on adoption of agricultural water management practices (Rainwater harvesting, Irrigation Drought resistant crops). Cross-sectional survey design was adopted, while proportionate and simple random sampling technique was used to obtain the respondents. The accessible population was 6,230 smallholder farmers from the target population of 26,804 smallholder farmers in Rongai sub county Kenya. The study was done in August 2023 to November 2023.The study included 120 smallholder farmers in Rongai Sub County. The study used questionnaire to collect data while binary logistic regression was used to analyze the data. The results indicated that adoption of agricultural water management practices is low, only 36% of smallholder farmers had adopted agricultural water management practices. The P value calculated for the 120 smallholder farmers was P=.033 which is < than .05 and therefore the null hypothesis was rejected concluding that in this study income level had statistically significant influence on adoption of agricultural water management practices among smallholder farmers in Rongai sub-county in Nakuru ,Kenya. The findings may help in emphasizing the necessity of assisting farmers in removing financial obstacles that may hinder adoption of agricultural water management practices.

Rosaceae in the Mountain-Xerophyte and Steppe Vegetation of Shahbuz District, Current Status of the Woody Species

Rosaceae spreading in mountain-xerophytic and steppe vegetation of Shahbuz district flora, is presented in the article. Information is given on the taxonomic composition, life forms, ecological groups, geographical area types and classes of woody species of the family. According to the researches conducted by us, when we characterized the woody species of the mountain-xerophyte and steppe zone of the Shahbuz district flora belonging to the Rosaceae family by genera, it was found that Rosa — 21 (37.5%), Crataegus — 7 (12.5%), Pyrus — 7 (12.5%), Prunus — 6 (10.71%), Sorbus — 5 (8.92%), Cotoneaster — 4 (7.14%), Rubus and Spiraea — 2 (3.57%), Malus and there is — 1 (1.78%) species of the Pyracantha genus. Shrub plants, especially woody species of the Rosaceae family, occupy an important place in the structure of Shahbuz district flora. They are part of the bush steppes, form independent groups and form unique formations.

Challenging a decades-old paradigm: ProB and ProA do not channel the unstable intermediate in proline synthesis after all

The pathway for synthesis of proline in most forms of life produces a highly unstable intermediate, γ-L-glutamyl 5-phosphate (GP). For nearly 70 y, channeling of this intermediate from the active site of glutamate 5-kinase to the active site of GP reductase has been believed to protect GP from cyclization to a dead-end product. However, the evidence presented in support of this idea is not conclusive. We show that changes in the structures of the kinase or reductase that should preclude a protein-protein interaction do not compromise proline synthesis in Escherichia coli, demonstrating that channeling does not occur. We calculate that the half-life of GP is 320 ms. Although GP is indeed unstable, it should diffuse the length of an E. coli cell in less than 3 ms. Thus, most GP produced by glutamate 5-kinase should encounter the active site of GP reductase before cyclization occurs.

Unveiling the Ecological and Pharmacological Perspectives of Lithophytic Life Form

Environmental extremes such as high temperature, cold, alkalinity, drought, and rocky substrate modulate plant growth and promulgate adaptive characteristics in plants. The same plants can be found inhabited in different life forms, namely epiphytic, lithophytic, terrestrial, halophytic, and psammophytic, based upon adaptive characteristics essential for their survival. Microorganisms residing on the surface or within rocks make a unique habitat referred to as “lithobiontic habitat,” which further facilitates weathering phenomena and accumulates water and nutrients from the ecosystem to form a lithobiontic ecological niche. Lithobionts are divided into two groups, namely epiliths and endoliths, based on their presence on rock surfaces. Lithophytes, the rock-inhabiting plants, thriving under extreme stress conditions adapted successfully and their extraordinarily challenging atmosphere encouraged them to make new forms of life. Many biotic and abiotic factors are responsible for dwelling in such environments. Lithophytes interact with their surroundings to obtain vital elements necessary for their metabolism and, because of natural selection, their morphology and physiology help them with better adaptation. Another factor that facilitates such adaptation in lithophytes is the association of a fungus with lithophytic roots to ensure nutrient supply for their survival and facilitate growth in extreme conditions. Arbuscular mycorrhizal (AM) root fungal associations commonly found among plants under stressed conditions help hosts adapt and promote plant life. In lithophytes, AM association aids in nutrient uptake from soil and facilitates plant survival under harsh conditions. The present review provides a comprehensive account of lithophytic life forms and covers different aspects of rock-dwelling plants concerning their historical background, classification, habitat, distribution in various families, root adaptive mechanisms, and phytopharmacological status. This review focused on plant species that can be considered among the most common representatives of lithophytes. This manuscript furnishes insights into different perspectives on the ecological and biological properties of the most common lithophytic species adapted to extreme rocky structures and explains their wide array of applications for pharmacological use.

The Tragedy of the confrontation: the religious factor in the Russian Civil War

The article analyzes a collective monograph edited by A. S. Puchenkov and devoted to the consideration of the role and significance of the religious factor during the Civil War in Russia. According to the reviewer, the authors, based on the unique material collected in 22 Russian and foreign archives, were able to show and prove the need to take into account the phenomenon of the religious factor, rightly defining it as «a set of many components affecting the social, socio-political, spiritual, cultural and national life of society, determining the order of functioning of religion and its institutions in the system of relations between the state and society, as well as the influence of religion as a social institution on non-religious forms of public life». According to S. L. Firsov, the authors have made a successful attempt to draw a detailed picture of the state of religious denominations (primarily the Orthodox Russian Church) during the Civil War. In his opinion, the reviewed monograph can be called a kind of encyclopedia of the spiritual life of Russia during the Russian Troubles of the early 20-th century. The authors of the monograph made a worthy contribution to the study of the moral and moral state of Russian society by writing a deep and useful work for all those interested in both the history of the Orthodox Russian Church of the era of Revolution and Civil War, and the history of the Russian Troubles of the early twentieth century as a whole.

Wisdom of age is the main psychological geroprotector in older people (analytical review)

The article presents an analytical review of the problem of aging from the standpoint of social and spiritual forms of human life. The intensive growth in the number of elderly people has sharply reduced the quality of public attitude towards them. The foundations of social relations between elderly people and the state, intergenerational, family, and other relations have acquired a new colouring, in connection with which the importance of wisdom in solving these problems increases. The wisdom in an elderly person is potent of keeping his inner world in a state of balance. A wise person perceives his presence in life as a paradigm of integration, which determines the coexistence of all age phases during his life. It helps to cast aside regret about past failures, unfulfilled hopes, to find a lot of good even in a far from easy life. Wisdom is the universe to which a person should come in old age. Wisdom will always find something that will please and strengthen the spiritual strength of a person, make his life positions stable, lead away from despondency and a sense of helplessness. The wisdom of age creates self-confidence in an elderly person, pride in the years lived with dignity, in the benefits created, which are used by the young. The concept of “conscious aging” is based on inner strength and spiritual stability over old age. Of course, old age is full of sorrows, but life experience gives it prudence to endure them with dignity. Wisdom serves as a support for an aging person for the normal perception of all life changes, it constantly reminds him that life does not close its doors to him.

From Chaos to the Absurd: Existentialism for the 21st Century

As Sartre pointed out, philosophical questions are questions that each generation must ask themselves because only this promotes the feeling of being alive, which is especially true for existential questions closely related to time–space, the moment, and our society. Sartre placed his philosophy of existentialism in wartime and the social conditions of the time at the beginning of the 20th century. We can equate these conditions with today’s conditions; we are once again facing threats of war, and once again, we are facing chaotic conditions that increasingly lead to absurdity but are also entirely different. Today, at the beginning of the 21st century, the clarity and disambiguation of the 20th century no longer exist, as the relationships between beings and the world have drastically changed. We can observe that (1) the world is not one; there are two worlds, the physical and the cyber world and (2) being is not one; there are two beings (entities), human and AI-based forms of artificial life (ALF), between which there is a permanent tension. We advocate the thesis that in the society of the future, man must still play a master role; he must still be the being who will guide this society. Also, as Sartre claimed, each era must create its philosophy and consider real time–space. Responses to changes in this time–space also relate to existentialism in the 21st century. In this context, it is necessary to redefine the view of the future and the guidelines for the development of future society.

The Sociality of Form: Camillo Sitte's Urban Morphologies

AbstractThis article examines a text by a lesser‐known figure of fin‐de‐siècle Vienna, Camillo Sitte's Der Städtebau nach seinen künstlerischen Grundsätzen (1889), tracing the relationship between urban form and social structure in Sitte's treatise. It identifies the key points of this relationship in terms of causality: the form of public spaces can determine the form of social life; surrogacy: the throngs of the places’ inanimate inhabitants, the statues and monuments, are placeholders for the missing human gatherings; and finally displacement: the thick sociality of community life is transferred onto a heightened relationality with urban forms. Sitte's text sublimates the social; it diffuses abstract social structures—density, relationality, connection—onto the spatial morphologies of the city. Ultimately, this diffusion results in a capacious yet weakened model of sociality.

Geochemical Parameters of Oil and Rock Samples from Murzuq Basin, Libya: An Application for Paleoenvironment Description

Seven sedimentary rocks and seven crude oils undertook a geochemical analysis to describe their organic matter type and then tag the Paleoenvironments based on the organic matter. The samples were collected from B1-NC151, A1-NC58, P1- NC1, E1-NC174, D1-NC151, H29-NC115, and D1-NC174 boreholes, located in Murzuq Basin. The study was established to understand life forms in the ecosystem based on biomarkers that biochemical techniques recognized. Microscopy was used for kerogen typing and Spore Color Index reference. Organic matter (EOM) was extracted from cutting samples. Furthermore, hydrocarbons were saturated and aromatic fractionated from the samples to investigate using Gas chromatograph system. Spore color index (SCI), range between 1.5-3.5, Amorphous organic matter (AOM) from 42 to 95 percentage, Phytoclasts % were 4-15.5, and Palynomorphs % range 0.5 to 7.5. The percentage of Carbon 85.66-86.29, Hydrogen 13.16-13.73, Nitrogen 0.06 -0.27, and Sulfur 0.31-0.70, besides wax were measured, ranging among 1.3 to 6.0 %. Pristine/n-C17, Phytane/n-C18, Pristine/Phytane, carbon preference index measurements (CPI) and DBT/P as saturated hydrocarbon ratios from whole oil chromatographs were established. Tricyclic terpanes, hopanes and n-alkanes were distinguishing as the most important oil biomarkers that reserved the characteristics in the Paleoenvironments.

Transcriptional responses to diets without mineral phosphorus supplementation in the jejunum of two high-yielding laying hen strains

Phosphorus (P) is an essential mineral for all forms of life including laying hens, playing a crucial role in growth and efficient egg production. Recent studies suggest that current P recommendations might exceed the physiological demand, leading to unnecessarily high P excretions. This study on Lohmann Brown (LB) and Lohmann Selected Leghorn (LSL) laying hens (n=80; 10 replicates per strain, production period, and dietary group) investigates transcriptional changes in the jejunum, a critical intestinal segment for mineral absorption, in response to a diet either without (P-) or with (P+) a mineral supplement from monocalcium phosphate, administered over a 4-week period during the transition (15–19 weeks) or onset of laying (20–24 weeks). DESeq2 analysis of RNA sequencing data revealed that most differentially expressed genes (DEGs) varied between strains and age groups, with less pronounced effects from dietary mineral P content. The 19-week-old LB hens showed a stronger response to dietary mineral P removal, with transcripts affiliated with increased adaptation of the metabolism and decreased immune pathway activation. The identified pathways such as folate biosynthesis and p53 signaling, potentially link altered energy and amino acid metabolism (2-oxocarboxylic acid and arginine). Interestingly, genes involved in calcium transport (CALB1) and cellular signaling (PRKCA, STEAP4) along with tight junctions (CLDN2) were affected by complete removal of mineral P supplements, suggesting a promoted intestinal mineral uptake. Transcriptional regulation in the jejunum in response to low dietary mineral content is strain-specific when the laying phase begins, which may contribute to a physiological Ca:P ratio.

Animation, Automata, Biomimesis

The animation-effect of the movement-image constitutes not only a form of life but also a machinic analogue for Husserl's and Kant's accounts of temporality, engendering what Bernard Stiegler calls ‘a cinematography of consciousness’. If autonomous movement is the hallmark of the living being as well as the inanimate mechanism, the movement-image likewise subtends scenes of human and artificial intelligence. Pairing a cybernetic reading of Lacan's mirror stage essay with reflections on the self-recognition dance of Machina Speculatrix (an early example of a biomimetic robot), this essay considers how exosomatic feedback loops enable a kind of ipseity for machinic life as well as the human being as technical being. Despite adopting Derrida's ideas of originary prostheticity and automaticity, rendering living beings as always-already machinic life, Stiegler sets arche-cinema against arche-writing.

Suppression of a transcriptional regulator, HexA, is essential for triggering the bacterial virulence of the entomopathogen, Xenorhabdus hominickii

A nematode-symbiotic bacterium, Xenorhabdus hominickii, exhibits two distinct lifestyles. Upon infection of its host nematode into a target insect, X. hominickii is released into the insect hemocoel and becomes pathogenic. This study examines the critical transformation in bacterial life forms concerning the activity of a transcriptional regulator, HexA. When X. hominickii was cultured in tryptic soy broth, HexA was expressed during the stationary phase of bacterial growth. Conversely, HexA was expressed in the early growth stage within the insect host, Spodoptera exigua, when infected with X. hominickii. The transient expression of HexA was succeeded by the expression of another transcriptional regulator, Lrp, which led to the production of bacterial virulent factors. Expression of HexA was manipulated by replacing its promoter with an inducible promoter controlled by the inducer, l-arabinose. In the absence of the inducer, the mutant bacteria expressed HexA at a low level, resulting in a bacterial culture broth that was more effective at suppressing insect immune responses than the wild type. When the inducer was added, HexA was expressed at high levels, rendering the culture broth ineffective in immunosuppression. Interestingly, expression of HexA inhibited the expression of another transcriptional regulator, Lrp, which in turn induced the expression of a non-ribosomal peptide synthetase, gxpS, leading to the production of an immunosuppressive metabolite, GXP. Suppression of HexA expression in mutant bacteria augmented GXP levels in secondary metabolites. This indicates that infection of X. hominickii into the insect host represses HexA expression and upregulates Lrp expression, leading to GXP production. The GXP metabolites inhibit insect immunity, thus protecting the bacteria-nematode complex. Therefore, the suppression of HexA expression in the insect hemocoel is crucial for the bacteria’s transition from a symbiotic to a pathogenic life form.

Elevational patterns of species richness and phylogenetic diversity in a Mediterranean island

Understanding the abiotic factors influencing biodiversity patterns on Earth is a crucial task for conservation scientists. At the regional level, meso-climate factors, primarily associated with elevational gradients, are of great importance. However, disentangling these factors can be challenging due to the influence of other variables, such as geological substrata. To address this issue and better understand elevational gradients, it is essential to study geologically homogeneous terrains, particularly in Mediterranean islands where such research is lacking. In this study, we investigated the distribution of plant species richness along the elevational gradient of the Limbara massif, which consists predominantly of granite rocks and ranks as the third-highest peak in Sardinia at 1,359 meters a.s.l. We employed generalized linear models to analyze richness patterns, considering various factors, including all plant species, functional species groups categorized by Raunkiær life forms, chorological groups of species, alien species and phylogenetic diversity. Our findings revealed a hump-shaped model of species richness along the elevational gradient, with lower elevations exhibiting the highest species richness. Additionally, endemic species richness increased with higher elevations, while alien species were predominantly found at lower elevations. These results indicate that the Limbara massif possesses a significant elevational gradient in species composition, likely reflecting a unique plant evolutionary history. Furthermore, we emphasize the importance of published floras as valuable sources of biodiversity data for such studies.

Does succulence in woody plants delay desiccation, and is stored water used to maintain physiological function during drought conditions?

Succulence is a trait that describes water storage in plant organs and tissues regardless of life form. Plants use the stored water to maintain physiological function and delay desiccation. However, it is unclear whether succulence in leaves, stems and roots of woody plants delays desiccation, whether it provides 'utilizable water' to maintain physiological function, or buffers changes in water status in drying soils through capacitance. We conducted a pot dry-down experiment with nine shrub species to determine whether woody plants with greater leaf, stem, or root succulence have greater shoot utilizable water or capacitance. We also investigated whether greater succulence delays desiccation, represented by cumulative VPD, until evapotranspiration ceased or until utilizable water was exhausted. Greater leaf and stem succulence were strongly related to greater shoot utilizable water and capacitance. However, desiccation time was not delayed in plants with greater total shoot succulence, utilizable water, or capacitance. Instead, woody plants with greater root succulence had longer desiccation times. This suggests that woody plants use aboveground succulence to maintain physiological function and water status during drought, whereas root succulence extends desiccation time. Our study improves the mechanistic understanding of how woody plants use stored water to survive in dryland ecosystems.

When the world gets worse: Form and feeling in Australian climate fiction

In a political context of climate inaction, Australian fiction writers have increasingly focused on the emergency; drawing attention to how the political and environmental processes underway might play out and exploring attendant emotions. The novel form – with its foregrounding of private feelings and individual agency, and its historical alignment with the development of the extractive capitalism it may wish to critique – occupies an ambivalent position as a tool to bring about change. If the task is to refigure the social imaginary towards interconnection with all forms of life, is the novel an inadequate means of social engagement? I hope to make the case – by referencing debates in climate change literature and through discussion of Scary Monsters by Michelle de Kretser (2021) and This Devastating Fever by Sophie Cunningham (2022) – that writers are using the distinctive capacities of the novel to think through contemporary challenges. These novels, distinct in tone and narrative content, both treat climate change as inextricable from other forces – such as colonialism and misogyny – and emphasise interconnectedness with other beings. Through a commitment to play, via formal experimentation and humour, they help readers to engage with the intellectual and emotional challenges of a changing world.

Protocells by spontaneous reaction of cysteine with short-chain thioesters.

All known forms of life are composed of cells, whose boundaries are defined by lipid membranes that separate and protect cell contents from the environment. It is unknown how the earliest forms of life were compartmentalized. Several models have suggested a role for single-chain lipids such as fatty acids, but the membranes formed are often unstable, particularly when made from shorter alkyl chains (≤C8) that were probably more prevalent on prebiotic Earth. Here we show that the amino acid cysteine can spontaneously react with two short-chain (C8) thioesters to form diacyl lipids, generating protocell-like membrane vesicles. The three-component reaction takes place rapidly in water using low concentrations of reactants. Silica can catalyse the formation of protocells through a simple electrostatic mechanism. Several simple aminothiols react to form diacyl lipids, including short peptides. The protocells formed are compatible with functional ribozymes, suggesting that coupling of multiple short-chain precursors may have provided membrane building blocks during the early evolution of cells.