Rural Forest Areas Research Articles

How to maximize the joint benefits of timber production and carbon sequestration for rural areas? A case study of larch plantations in northeast China

BackgroundImplementing large-scale carbon sink afforestation may contribute to carbon neutrality targets and increase the economic benefits of forests in rural areas. However, how to manage planted forests in China to maximize the joint benefits of timber production and carbon sequestration is still unclear. Therefore, the present study quantified the effects of different rotation lengths, thinning treatments, site quality (SCI), stand density (SDI), and management costs on the joint benefits of carbon sequestration and timber production based on a stand-level model system developed for larch plantations in northeast China.ResultsThe performances of the different scenarios on carbon stocks were satisfactory, where the variations in the outcomes of final carbon stocks could be explained by up to 90%. The joint benefits increased significantly with the increases of SDIs and SCIs, regardless of which rotation length and thinning treatments were evaluated. Early thinning treatments decreased the joint benefits significantly by approximately 131.53% and 32.16% of middle- and higher-SDIs, however longer rotations (60 years) could enlarge it by approximately 71.39% and 80.27% in scenarios with and without thinning when compared with a shorter rotation length (40 years). Discount rates and timber prices were the two most important variables affecting joint benefits, while the effects of carbon prices were not as significant as expected in the current trading market in China.ConclusionsThe management plans that promote longer rotations, higher stand densities, and no thinning treatments can maximize the joint benefits of carbon sequestration afforestation and timber production from larch plantations located in northeast China.Graphical

Analysis of carbon and nitrogen from atmospheric sources by bulk deposition sampling at various locations in Germany

Atmospheric deposition of particulate matter is an important indicator of air pollution and a significant factor in material surface fouling. The elemental composition of this nutrient-containing dust depends largely on the exposure region and time as well as on climate. Therefore, in this paper we report an analysis of atmospheric pollutions with a self-made low-cost bulk deposition sampler directed at sampling deposition via air transport and rainfall. We used the device in diverse environments - thus comparing an urban region, an area surrounded by forest and an area mainly dominated by agriculture. The total organic carbon (TOC) and total nitrogen (TN) amounts were selected as indicator parameters and analyzed in a biweekly rhythm for three and a half and two years, respectively. The TOC value responded to particulate matter in the urban area, especially significant were the influences of the New Year's firework in urban and pollen in the rural forest area. In contrast, the TN value was more under the influence of the nitrogen emissions in the agriculture-dominated area. However, the TN value did not correlate with the NOx values in the urban area because the atmospheric nitrogen emissions in the city might originate from various emission sources. Summarizing, the TOC and TN values of the self-made low-cost bulk deposition sampler were in good agreement with environmental events of their immediate surrounding. Moreover, the selected containers and sampling procedures are universally applicable to monitor and analyze organic as well as inorganic parameters (e.g. metal ions) of atmospheric deposition.

Urban–Rural Comparisons of Biogenic Volatile Organic Compounds and Ground-Level Ozone in Beijing

Ground-level ozone (O3) pollution has been a severe environmental and health problem for decades. The importance of biogenic volatile organic compounds (BVOCs) in the formation of tropospheric photochemistry O3 has been highlighted, especially in areas of rapid urbanization. We conducted simultaneous measurements of trace gases, including NO, NOX, O3, and BVOCs (i.e., isoprene and α-pinene), in the urban and rural forest areas of Beijing to determine the relationships between them. The results highlight the differences between the urban and rural forest areas of Beijing in terms of ambient air concentrations of BVOCs and O3, and the interrelationships between BVOCs, NOX, and ozone were quantified. Moreover, the isoprene concentration was found to be higher in the atmosphere of the urban site than of the rural site, which had higher α-pinene concentrations and higher O3 concentrations. The NOX concentration was higher at the urban site than at the rural site, and there was a significant exponential relationship between NOX and O3 at the urban site, indicating that the impact of NOx on O3 at the urban site was greater than that at the rural site. The O3 concentration increased with rising isoprene and α-pinene in both sites. In the case of substantially increased BVOC concentrations, declining NOX concentrations strongly promote the formation of O3. Consideration should be given to planting tree species with low-BVOC emissions, as they are crucial for mitigating O3 pollution in urban areas. Additionally, the relationships between BVOCs, NOX, and O3 should be considered in policymaking related to O3 control.

Changes in Soil Fungal Diversity and Composition along a Rural–Urban Gradient

The functioning of forest ecosystems depends on the taxonomic and ecological diversity of soil fungi. Urbanization is increasing worldwide and is regarded as a key driver of environmental change altering local species assemblages in urban forest. We investigated whether the degree of urbanization and local forest characteristics affect the soil fungal community in 20 beech forests located along a rural–urban gradient in the city of Basel and its suburbs (Switzerland). We analyzed their soil fungal communities by DNA metabarcoding of the rDNA ITS2 region and related these data to local forest vegetation characteristics and soil properties. The number of fungal OTUs in the 20 forests examined ranged from 170 to 303. Richness, diversity and evenness of fungal communities were all significantly affected by the degree of urbanization, but in different ways. Soil fungal richness was highest in forests in areas with a low degree of urbanization and lowest in forests in rural areas. In contrast, the fungal community diversity increased with the increasing degree of urbanization. Different fungal phyla and fungal guilds showed distinct patterns in their relative abundance along the rural–urban gradient. The degree of urbanization reduced the relative abundance of symbiotrophic fungi, but increased that of saprotrophic and pathotrophic fungi. Our results show that urbanization changes soil fungal community, which in turn can lead to alterations in forest ecosystems.

Preventing Animals Interference using Intelligence Camera and RFID System

Animals’ interference into operation centers has become major headaches for some companies. Although it is a trivial case, the animals’ interference has disrupted major operations of the company. The article takes case of an Indonesian utility company, PN, that has challenges to distribute electricity to nationwide, especially in the rural areas, mountain, and forest areas, and thousand islands. Those areas are surrounded by animal presences that might trespass and interference into distribution sites such as transmission lines and transformers. The common presence of animals’ interference was found such as snakes, birds, and squirrels have created blackouts in some areas. The article proposes the use of intelligence cameras and RFID systems, along with preventive devices to assist animal trespassing in the distribution area. The article takes pilot of project in Payakumbuh area that is in West Sumatera, that has high animal interferences to the distribution system. Since the last decade, PN has promoted digital transformation in all her business units. The use of digital technology is expected to provide solution reference for animal interfere cases around Indonesia area, considering that each area in Indonesia has an un

Towards the invasion of wild and rural forested areas in Gabon (Central Africa) by the Asian tiger mosquito Aedes albopictus: Potential risks from the one health perspective.

Since its first record in urban areas of Central-Africa in the 2000s, the invasive mosquito, Aedes albopictus, has spread throughout the region, including in remote villages in forested areas, causing outbreaks of Aedes-borne diseases, such as dengue and chikungunya. Such invasion might enhance Ae. albopictus interactions with wild animals in forest ecosystems and favor the spillover of zoonotic arboviruses to humans. The aim of this study was to monitor Ae. albopictus spread in the wildlife reserve of La Lopé National Park (Gabon), and evaluate the magnitude of the rainforest ecosystem colonization. From 2014 to 2018, we used ovitraps, larval surveys, BG-Sentinel traps, and human landing catches along an anthropization gradient from La Lopé village to the natural forest in the Park. We detected Ae. albopictus in gallery forest up to 15 km away from La Lopé village. However, Ae. albopictus was significantly more abundant at anthropogenic sites than in less anthropized areas. The number of eggs laid by Ae. albopictus decreased progressively with the distance from the forest fringe up to 200m inside the forest. Our results suggested that in forest ecosystems, high Ae. albopictus density is mainly observed at interfaces between anthropized and natural forested environments. Additionally, our data suggested that Ae. albopictus may act as a bridge vector of zoonotic pathogens between wild and anthropogenic compartments.

Molecular Identification of Enteric Viruses in Domestic Animals in Northeastern Gabon, Central Africa.

Astroviruses (AstVs), enteroviruses (EVs), and caliciviruses (CaVs) infect several vertebrate taxa. Transmitted through the fecal-oral route, these enteric viruses are highly resistant and can survive in the environment, thereby increasing their zoonotic potential. Here, we screened for AstVs, EVs, and CaVs to investigate the role of domestic animals in the emergence of zoonoses, because they are situated at the human/wildlife interface, particularly in rural forested areas in Central Africa. Rectal swabs were obtained from 123 goats, 41 sheep, and 76 dogs in 10 villages located in northeastern Gabon. Extracted RNA reverse-transcribed into cDNA was used to detect AstVs, EVs, and CaVs by amplification of the RNA-dependent RNA polymerase (RdRp), or capsid protein (VP1) gene using PCR. A total of 23 samples tested positive, including 17 goats for AstVs, 2 goats, 2 sheep, 1 dog for EVs, and 1 dog for CaVs. Phylogenetic analyses revealed that AstV RdRp sequences clustered with sheep-, goat-, or bovine-related AstVs. In addition, one goat and two sheep VP1 sequences clustered with caprine/ovine-related Evs within the Enterovirus G species, and the CaV was a canine vesivirus. However, human-pathogenic Evs, EV-B80 and EV-C99, were detected in goats and dogs, raising questions on the maintenance of viruses able to infect humans.

Assessing the Sustainability of NTFP-Based Community Enterprises: A Viable Business Model for Indonesian Rural Forested Areas

Indonesia’s vast forested areas have the potential to serve as a crucial source of livelihood for local communities. However, the current contributions of these forests to community livelihoods are significantly underutilized in comparison to their potential. This study evaluates the financial performance and sustainability of community forest-based businesses operating around the forest management area of the Lakitan-Bukit Cogong forest management unit (LBC FMU). Data were collected through semi-structured and in-depth interviews with the key informants through purposive sampling. Financial viability analysis and a qualitative approach were used to assess the feasibility of the businesses. The findings revealed that all businesses show positive values for all financial indicators. From profit estimation and value-added distribution, all products are shown to be feasible. Concerning the value and supply chain, the rubber-processing industry has a remarkable flow. Moreover, small forest enterprises (SFEs) highlight natural capital optimization through multitudinous derivatives of products that could support a substantial regenerative economy, including citronella essential oil, native honeybees, rubber-based product, biochar, skewers, and liquid smoke. In addition, the multidimensional scaling and rapid appraisal for forest (MDS-RAPForest) approach generates a result based on multiple dimensions (ecology, economics, social and human resources, and institutional and administrative dimensions) showing that overall, SFEs are categorized as sufficient/quite sustainable. Furthermore, mainstreaming adaptable forest-based enterprises, jurisdiction approaches, and cross-production system strategies are also discussed. Our findings suggest that sustainable NTFP-based activities within a community context can be facilitated through interconnected market systems, appropriate price regulations, and support from stakeholders and legal frameworks.

Resin tapping influence on maritime pine growth depends on tree age and stand characteristics

Resin is a renewable forest resource that can increase the economic value of some forests in rural areas. Resin production is associated with climatic conditions. However, its impact on trees’ growth remains unclear. Here, we studied radial growth in six Portuguese Pinus pinaster forests that had been resin tapped for 5–7 years along a latitudinal and climatic gradient to understand whether resin tapping affects tree growth, and how it is affected by climate, stand and tree traits. Tree-ring width (TRW) on tapped and untapped trunk sides was compared before and during the tapping period. Tree-ring width decreased in the three youngest populations (< 30 years), with no changes in older populations (> 40 years), while TRW increased after resin harvesting began in the oldest stand (> 55 years). Annual resin-tapping impact (RTI), calculated as the ratio between TRW during tapping years and the 5-year average TRW before tapping, was below and above 1 in the younger and older stands, respectively. Among stand characteristics and across sites, RTI was negatively correlated with tree competition and positively correlated with TRW, cambial age, and tree height. Climatic conditions had a minor role on tree growth response to resin tapping. Our main conclusion is that the effect of resin extraction on growth is age-dependent. Our results encourage the co-production of resin and wood on maritime pine stands over 40 years old where resin extraction impact on growth is negligible.

Rural tourism and evolving identities of Chinese communities in forested areas

Rural China has faced considerable challenges in recent decades, leading to the dissolution of many state-owned forest farm communities and threatening sustainable development. There remains a need to understand the drivers and characteristics associated with these changes, including the influence of rural tourism on combating forest recession. This study addresses these concerns by constructing and applying a regional sociology framework to investigate the link between tourism and the evolution of communities in rural forested areas. Focusing on forest farm communities in central China, recent findings indicate that tourism has complicated social relations, leading to a transformation of identity from a predominantly status-based, industrial, and cultural identity to a comprehensive regional identity. The study aims to enrich tourism and identity theory, providing theoretical and practical guidance for understanding and enhancing forest community development and sustainability through rural tourism in China and elsewhere.

The Production of Safety School Space from Climate Disasters in Doi Mae Salong Forest, Upland Northern Thailand

This research is conducted in Santikhiri, a hilltop village on the highest peak in the Doi Mae Salong forest, where climate change increases the intensity and frequency of natural disasters that immensely affect the local children in the mountainous area in Chiang Rai province, northern Thailand. There is only one secondary-level school in this forest landscape educating around 900 schoolchildren from various minority hill-tribe ethnic groups. This paper examines everyday life experiences recentering the village school's role as the producer of safe space for the forest children from climate disasters. School safety is a global framework for recognizing the importance of child-centered efforts in building disaster resilience for the education sector. Parameters and variables used to measure the disaster resilience of schools are adapted from the Climate Resilience Model and School Safety Model by Tong et al. (2012), covering three dimensions: 1) institutional issues, 2) physical conditions, and 3) external relationships. Lefebvre's Spatial Triad Framework is applied to dialectically interconnect dimensions to produce a safe space at the village school to protect the students from climate disaster threats. A mix-method method is applied with several techniques to collect data, including participant observation, semi-structured interviews, and content analysis. Furthermore, a scale Likert survey examined statements on school safety from educational practitioners in the rural forest area. The research argues that the production of safe space at the school is intertwined with budget allocation for disaster preparedness and response (institutional issue as l'espace concu), environmental protection campaign to create a hygienic school environment (physical conditions as l'espace percu), and support from the local community (external relations as l'espace vecu). However, the school is also two contradicting spaces of conceived and lived. Through the critical examination of the production of safe space, the school is a planned space of hierarchical power relations in institutional issues focusing on impacts from rapid-onset disasters. Concurrently, the forest children are still marginalized from external relationships and natural conditions' slow-onset climate change impacts.

An assessment of spatial distribution and atmospheric concentrations of ozone, nitrogen dioxide, sulfur dioxide, benzene, toluene, ethylbenzene, and xylenes: ozone formation potential and health risk estimation in Bolu city of Turkey.

Atmospheric pollutants including ozone, nitrogen dioxide, sulfur dioxide, and BTEX (benzene, toluene, ethylbenzene, and xylenes) compounds were evaluated concerning their spatial distribution, temporal variation, and health risk factor. Bolu plateau where sampling was performed has a densely populated city center, semi-rural areas, and forested areas. Additionally, the ozone formation potentials of BTEXs were calculated, and toluene was found to be the most important compound in ground level ozone formation. The spatial distribution of BTEXs and nitrogen dioxide pollution maps showed that their concentrations were higher around the major roads and city center, while rural-forested areas were found to be rich in ozone. BTEXs and nitrogen dioxide were found to have higher atmospheric concentrations in winter. That was mostly related to the source strength and low mixing height during that season. The average toluene to benzene ratios demonstrated that there was a significant influence of traffic emissions in the region. Although there was no significant change in sulfur dioxide concentrations in the summer and winter seasons of 2017, the differences in the spatial distribution showed that seasonal sources such as domestic heating and intensive outdoor barbecue cooking were effective in the atmospheric presence of this pollutant. The lifetime cancer risk through inhalation of benzene was found to be comparable with the limit value (1 × 10-6) recommended by USEPA. On the other hand, hazard ratios for BTEXs were found at an acceptable level for different outdoor environments (villages, roadside, and city center) for both seasons.

Diverging patterns at the forest edge: Soil respiration dynamics of fragmented forests in urban and rural areas.

As urbanization and forest fragmentation increase around the globe, it is critical to understand how rates of respiration and carbon losses from soil carbon pools are affected by these processes. This study characterizes soils in fragmented forests along an urban to rural gradient, evaluating the sensitivity of soil respiration to changes in soil temperature and moisture near the forest edge. While previous studies found elevated rates of soil respiration at temperate forest edges in rural areas compared to the forest interior, we find that soil respiration is suppressed at the forest edge in urban areas. At urban sites, respiration rates are 25% lower at the forest edge relative to the interior, likely due to high temperature and aridity conditions near urban edges. While rural soils continue to respire with increasing temperatures, urban soil respiration rates asymptote as temperatures climb and soils dry. Soil temperature- and moisture-sensitivity modeling shows that respiration rates in urban soils are less sensitive to rising temperatures than those in rural soils. Scaling these results to Massachusetts (MA), which encompasses 0.25Mha of the urban forest, we find that failure to account for decreases in soil respiration rates near urban forest edges leads to an overestimate of growing-season soil carbon fluxes of >350,000Mg C. This difference is almost 2.5 times that for rural soils in the analogous comparison (underestimate of <143,000Mg C), even though rural forest area is more than four times greater than urban forest area in MA. While a changing climate may stimulate carbon losses from rural forest edge soils, urban forests may experience enhanced soil carbon sequestration near the forest edge. These findings highlight the need to capture the effects of forest fragmentation and land use context when making projections about soil behavior and carbon cycling in a warming and increasingly urbanized world.

A study on the clinical, epidemiological profile and the outcome of the snake bite victims in kashmir valley.

Background:India is estimated to have the highest snakebite mortality in the world. In India, around 250,000 incidents of snake bite are reported annually with 50,000 deaths. There are about 236 species of snakes in India, most of which are nonpoisonous. Most snakes found in Kashmir valley are nonvenomous of colubridae family (ptyas mucosus or grass snake).Materials and Methods:This Hospital-based retrospective and prospective study was conducted at Sher-i-kashmir Institute Of Medical Sciences Srinagar, a multispeciality teaching hospital having clinics for various medical and surgical specialities. All snake bite patients admitted in department of Emergency Medicine, other specialities, and critical care medicine were taken in this study over a period of 3 years.Results:Among a total of 108 cases of snake bite, majority were males (57.40%) and mostly 20 to 50 years of age (68.51%).Majority of victims were from rural areas (93.5%) and most of the bites occurred during day time (87.96%) mainly on the lower limbs (63.88%). Highest number of cases occurred from May to October (98.14%). Most of the victims were farmers. Bleeding was present in 13.88% patients and coagulopathy ( International normalized ratio (INR)>1.2) in 63.88% of patients. Neuroparalytic features were present in 8.33% patients. Mortality in our study was 2.8%.Conclusion:Snake bite in our valley is an occupational hazard affecting working class engaged in farming in fields, fruit orchards, and forests in rural areas. There is seasonal variation with most envenomations occurring in peak months of agricultural activities. Non recommended first aid measures like tourniquet and incision are commonly encountered. Regular public health programmes regarding prevention, prehospital management (first aid), and immediate transfer to hospital should be emphasized.

Addressing energy injustice in rural landscapes: Community leadership, indigenous villages, and micro-hydro diffusion in Indonesia

The rapid development of renewable energy projects provides opportunities to livelihood around protected forests in rural areas. Living remotely with limited access to electrification has compelled villagers to establish renewable energy projects based on available resources. However, few studies have investigated actors' roles at the science-policy interface of such community-based renewable energy projects. This article describes the successful implementation of renewable energy projects in an indigenous village, Kasepuhan Ciptagelar, in Mount Halimun Salak National Park, Indonesia. Expert interviews and document analysis using the theoretical framework of the research-integration-utilization model revealed the role of various actors in the development of renewable energy projects in this indigenous village. Combining scientific research with indigenous information increased the villagers' acceptance of and willingness towards sustainably managing micro-hydro projects. Furthermore, grassroots community leaders successfully introduced the use of micro-hydro projects. Based on these results, we suggest employing a collaborative approach involving actors at different levels of energy and forest governance (local, regional, national, and international) as a promising tool to tackle energy and environmental injustice.

Demand for mycotourism products in rural forest areas. A choice model approach

ABSTRACT Mycotourism is an innovative tourist product linked to rural tourism and offered as an alternative to the use of a forest resource. Here we aim to identify which tourism products have a connection to mycotourism and which mycological activities most attract people to the product. Results identify wine tourism, religious tourism, and spa tourism as the tourism products most closely connected to mycotourism. Visiting truffle festivals, mycological parks and hiring a mycological guide are the most frequent activities. Practical applications of the study include marketing that can be used by public policymakers and private companies alike to promote the area.

Potential Hurricane Wind Risk to US Rural and Urban Forests

Abstract Hurricanes cause billions of dollars in damage annually in the United States and are projected to increase in intensity in the coming years. By exploring historical patterns of hurricanes and exposure of these hurricane-force winds across the landscape, areas of potentially high threat to future hurricane winds can be revealed. Combining potential threats from hurricane winds with forest data reveal the areas with the greatest potential threats to this important resource. Not surprisingly, most of the threats to forests occur in the Southeast, but the greatest threats to urban forests overall occur along the Northeast coast and in metropolitan centers in the Southeast. Overall, 4.6% of the nation’s rural forest basal area is within zones of the highest risk from hurricane winds. Information on regional and local scale variations in hurricane risk can be used to aid policies and local forest management to improve forest health and sustainability.

Teknik Pemanfaatan Biji Buah Pohon Raja (Pangium edule) Sebagai Bahan Makanan Oleh Masyarakat Kampung Kumurkek, Distrik Aifat, Maybrat-Papua Barat

In rural forest areas, plants and animals provide communities’ daily needs as well as marketable products that generate income for local communities. Healthy forests not only provide important ecological functions but also contribute to an indigenous population’s economy and culture. Papua is an important region in the world to study the social importance of tropical rainforests, as this vast region contains over 250 ethnic groups, most of whom depend on forest ecology for their livelihood. This research was aims to understand the process and utilization technique of Raja’s seeds (Pangium edule) as a traditional food source according to the indigenous knowledge of the Aifat people in Kumurkek, Aifat sub-District, Maybrat Regency. The study uses descriptive methods, field observations, and semi-structured interviews. Results indicate that there are six stages of Raja’s seeds processing, including seed harvesting, poaching, slicing, soaking in a river, and culminating in consumption. Tools and materials used in processing the seeds include machetes, wood, stone, and metal pans. As of now, there is no conservation scheme to preserve the Raja plant. However, the knowledge of Raja plant production is still bequeathed among relatives according to local tradition. Further research is needed in order to find other benefits of this species, such as use as a natural pesticide and other potential products that can provide additional income for the Aifat sub-District.

Diagnosing injuries caused by Indian wild boar: A case report

Wild boars are medium sized mammals commonly seen in Europe, Asia, North Africa and Greater Sun-da Island and India. Sus scrofa cristatus species is commonly seen in India. They are primarily nocturnal animal and are generally shy in nature, so incidences of attack by wild boar are rare. They are dangerous due to their razor sharp tusks, which can cause serious injuries. Wild boar attacks are under reported in the medical litera-ture. Attacks on humans by wild animals causing fatal injuries are not uncommon in rural and forest areas of India. This is the case of a 55-year-old male farmer who was assaulted from by an adult wild boar. Autopsy ex-amination revealed multiple penetrating injuries, bilateral rib fractures and an anorectal injury. Unlike the inju-ries inflicted by wild cats, canines and bulls, the hallmark of boar attack is the infliction of multiple penetrating injuries to the lower part of the body. As the victims of wild boar attack are usually recovered from rural forest areas, the investigating officers could be misled as to the nature of infliction of these multiple, fatal penetrating injuries to a possible homicide. This case is reported for its rarity, for the awareness of the possible injuries in such unnatural deaths, and for the factors predisposing to a boar attack. It is concluded that fatal penetrating injuries caused by this type of attack can be associated with extensive soft-tissue damage despite externally appearing to be simple puncture wounds. Keywords: Wild boar, penetrating injuries, anorectal injury

High HIV burden and recent transmission chains in rural forest areas in southern Cameroon, where ancestors of HIV-1 have been identified in ape populations

We studied HIV prevalence and genetic diversity in rural forest areas in Cameroon, where chimpanzee and gorilla populations infected with the ancestors of the different HIV-1 groups have been identified and transmitted to humans during the 20th century.A total of 2812 individuals were studied, 924 from south-central, 1116 from south-east and 772 from south-west Cameroon. Of 208 (7.4%) samples that were confirmed for HIV-1 infection all belong to HIV-1 group M. In all sites and in all age categories, HIV-1 prevalence was higher in women (160/1599 (10.0%)) as compared to men (48/1213 (4.0%)) with the highest prevalence in women aged between 25 and 34 years (>17%). For 188/208 (92.3%) HIV-1 positive individuals, a fragment of the pol gene was successfully amplified and sequenced. Phylogenetic analysis showed predominance of CRF02_AG (58%), a large diversity of subtypes (A, D, F2 and G), nine different CRFs and more than 12% URFs. Interestingly, 35/188 (18.6%) HIV-1 strains form 12 recent transmission chains. The majority of the clusters are composed of two (n = 8) or three (n = 3) sequences but one cluster included ten HIV-1 strains from women living in four different villages on a major road for logging concessions in the south-east (60 km distance).In the three regions of Cameroon where the ancestors of the four HIV-1 groups have been transmitted to humans, we observed a high HIV prevalence, especially in the southeast where HIV-1 M originated. Many factors allowing rapid establishment in the human population and subsequent rapid spread to urban areas of a new retrovirus or other pathogens of zoonotic origin are now present. Our study shows clearly that some rural areas should also be considered as hot-spots for HIV infection. Prevention efforts together with growing access to HIV diagnosis and antiretroviral treatment are urgently needed in these remote areas.