Brazilian Forest Research Articles

Uncommon Diet of the Toad Rhinella marina (Anura: Bufonidae) in a Rural Zone of the Amazon Forest in Brazil

Uncommon Diet of the Toad Rhinella marina (Anura: Bufonidae) in a Rural Zone of the Amazon Forest in Brazil

Impact of forest fire on the mercury stable isotope composition in litter and soil in the Amazon

Mercury (Hg) emissions from forest fires, especially tropical forests such as the Amazonian forest, were shown to contribute significantly to the atmospheric mercury budget, but new methods are still necessary to improve the traceability and to reduce the great uncertainties related to this emission source. Recent studies have shown that the combustion process can result in Hg stable isotope fractionation that allows tracking coal combustion Hg emissions, as influenced by different factors such as combustion temperature. The main goal of the present study was, therefore, to investigate for the first time the potential of Hg stable isotopes to trace forest fire Hg emissions and pathways. More specifically, small-scale and a large scale prescribed forest fire experiments were conducted in the Brazilian Amazonian forest to study the impact of fire severity on Hg isotopic composition of litter, soil, and ash samples and associated Hg isotope fractionation pathways. In the small-scale experiment, no difference was found in the mercury isotopic composition of the samples collected before and after burning. In contrast, the larger-scale experiment resulted in significant mass dependent fractionation (MDF δ202Hg) in soils and ash suggesting that higher combustion temperature influence Hg isotopic fractionation with the emission of lighter Hg isotopes to the atmosphere and enrichment with heavier Hg in ashes. As for coal combustion, mass independent fractionation was not observed. To our knowledge, these results are the first to highlight the potential of forest fires to cause Hg isotopic fractionation, depending on the fire severity. The results also allowed to establish an isotopic fingerprint for tropical forest fire Hg emissions that corresponds to a mixture of litter and soil Hg isotopic composition (resulting atmospheric δ202Hg, Δ200Hg and Δ199Hg were −1.79 ± 0.24‰, −0.05 ± 0.04‰ and −0.45 ± 0.12‰, respectively).

Quantifying timber illegality risk in the Brazilian forest frontier

Illegal logging remains widespread across the tropics, leading to extensive forest degradation and trade in illegal timber products. By adapting environmentally extended input–output modelling to timber originating from Brazilian native forests, we demonstrate how distinct illegality risks can be mapped and quantified at species-level across the supply chain. We focus on high-value ipê hardwood from the Amazon state of Pará, a leading producer of timber and contested forest frontier. Data on logging permits and state- and national-level Document of Forest Origin licences are used to estimate illegality risks due to missing or invalid logging permits, overstated ipê yields or discrepancies resulting from missing inflows of legal timber. We find that less than a quarter of all ipê entering supply chains between 2009 and 2019 is risk-free and highlight diversified strategies for the laundering of illegal timber across geographies. While legality does not ensure sustainability, this information can be leveraged to this end by supporting improved implementation and enforcement of forest regulations.

Biochemical characterization and mycorrhizal fungal community of plant species in the Brazilian seasonal dry forest.

Invasive alien plant species (IAPS) have the ability to change the biochemical properties and the arbuscular mycorrhizal fungal (AMF) community structure in their rhizosphere. Organic acids, microbial activity, and AMF play a key role in the invader's spread and also has interactions with the soil chemical factors. Our aim here was to assess the rhizosphere's biochemical factors, AMF community composition, and soil chemical properties associated with Cryptostegia madagascariensis (IAPS) and Mimosa tenuiflora (endemic plant species) from the Brazilian Seasonal Dry Forest. The highest values of total glomalin (5.87 mg g-1 soil), root colonization (54.5%), oxalic and malic acids (84.21 and 3.01 μmol g-1 , respectively), microbial biomass C (mg kg-1 ), Na+ (0.080 cmolc kg-1 ), Ca2+ (7.04 cmolc kg-1 ), and soil organic carbon (4.59 g kg-1 ) were found in the rhizosphere of C. madagascariensis. We found dissimilarities on AMF community structure considering the studied plant species: (i) Racocetra coralloidea, Dentiscutata heterogama, Dentiscutata cerradensis, Gigaspora decipiens, and AMF's richness were highly correlated with the rhizosphere of M. tenuiflora; and (ii). The rhizosphere of C. madagascariensis was highly correlated with the abundance of Claroideoglomus etunicatum, Rhizoglomus aggregatum, Funneliformis mosseae, and Funneliformis geosporum. The results of our study highlight the importance of considering C. madagascariensis as potential hosts for AMF species from Glomerales, and a potential plant species that increase the bioavailability of exchangeable Na and Ca at semi-arid conditions.

Forest conservation and food production: cookies based on jatoba flour

The tropical forests in Brazil are going through a deforestation cut every year due to its replacement for activities mainly linked to livestock and modern agriculture, so for forest conservation it is necessary the valorization and valuation of forest products. In this way, this research was based on the utilization of the jatoba (Hymenaea courbaril L.) flour to produce "cookie-like" cookies. After the chemical characterization of the jatoba flour, cookies were elaborated with fortified wheat and jatoba flours. The cookies were evaluated in their physical, chemical, and sensorial characteristics. The moisture content of all the cookies (traditional, chocolate, coconut, and coffee) was not below 5%. The protein values of the coconut and chocolate flour obtained the highest contents (5.9%), followed by the jatoba flour with coffee (5.8%) and with contents (5.5%) for thetraditional jatoba flour. The pH values of the jatoba flours ranged from 6.48% for coconutflour, to 7.23% for traditional flour. The pH values of the flours presented differences among themselves, the jatoba flour fortified with coffee presented the lowest pH value. Concluding that the elaboration of the products allowed to prove that a level of addition of up to 5% of jatoba flour in cookies which was well accepted by the evaluators, obtaining sensorial acceptance. Thus, the results show an innovative product using the jatoba to produce foods rich in fiber and protein, which values the species and its conservation in natural environments.

Brazilian Forest Code: evolution of environmental standards between 1934 and 2012

Legislative evolution in Brazil in the field of Environmental Law took place after the year 1972. In that year, one of the most important global conferences on the environment was held in Stockholm, and as a result of international pressure, new directions were taken in Brazilian environmental policy. Objective: The objective of this study is to analyze the evolution of environmental protection through constitutional legislation, presenting a comparative study among the Forest Codes that have been enacted in the country, namely those of 1934, 1964, and the current one from 2012. Materials and Methods: This study is of an applied and descriptive nature, employing methodological procedures such as bibliographic and documentary research.

Using land inequality to inform restoration strategies for the Brazilian dry forest

Forest and landscape restoration aims not only to restore ecosystems but to improve people's livelihoods. However, mapping of restoration often neglects key socioeconomic aspects such as land-access inequalities. In this article, we quantify and describe the distribution of vegetation deficits (areas that demand mandatory restoration) across small and large rural properties across 1,204 municipalities of the Brazilian Caatinga biome. We mapped 313,537 ha of vegetation deficit, i.e. areas previously cleared that will need to be restored for legal compliance. This vegetation deficit is almost equally shared between 141,144 smallholders and only 2,986 in large properties. Around half of this vegetation deficit is spatially clumped mainly in the East and along the main river basin of the region dominated by rich large-holders. On the other hand, spatially scattered and poor small landowners hold around another half of the vegetation deficit across several municipalities. Because of such an unequal distribution of native vegetation deficit, we propose three different restoration strategies to account for land-access inequalities and socioeconomic differences at the municipality level. This is one of the first studies to clearly address land inequality and socioeconomic profile of landowners to inform adapted restoration strategies. Restoration planning that ignores land concentration and socioeconomic contexts may reproduce inequalities known to be one of the main causes of ecosystem degradation across Global South countries.

Environmental management assessment in state forest concessions in the Brazilian Amazon

The environmental sustainability of Brazilian public forests under a concession regime has been the subject of controversy, highlighting the need for effective and continuous monitoring. This study evaluated the performance of stakeholders and efficiency of environmental monitoring of state forest concessions in the Eastern Amazon. Monitoring was evaluated based on compliance with the rules by public and private stakeholders and on their satisfaction perception. Forest monitoring efficiency and governance was analysed using international guidelines for concessions adapted to the local reality. Monitoring and forest governance were evaluated as partially satisfactory, with asymmetric performance among stakeholders. The robust legal and procedural framework in Brazil confers security to the forest concession regime. However, key elements such as field inspections, communication, transparency, and accountability need to be improved for effective environmental monitoring in state concessions. Safeguarding public forests under concessions, typically in remote areas and with weak institutions, would at least require the use of remote monitoring technologies and standardized administrative and accountability procedures.

Linking above and belowground carbon sequestration, soil organic matter properties, and soil health in Brazilian Atlantic Forest restoration

Forest restoration mitigates climate change by removing CO2 and storing C in terrestrial ecosystems. However, incomplete information on C storage in restored tropical forests often fails to capture the ecosystem's holistic C dynamics. This study provides an integrated assessment of C storage in above to belowground subsystems, its consequences for greenhouse gas (GHG) fluxes, and the quantity, quality, and origin of soil organic matter (SOM) in restored Atlantic forests in Brazil. Relations between SOM properties and soil health indicators were also explored. We examined two restorations using tree planting (‘active restoration’): an 8-year-old forest with green manure and native trees planted in two rounds, and a 15-year-old forest with native-planted trees in one round without green manure. Restorations were compared to reformed pasture and primary forest sites. We measured C storage in soil layers (0–10, 10–20, and 20–30 cm), litter, and plants. GHG emissions were assessed using CH4 and CO2 fluxes. SOM quantity was evaluated using C and N, quality using humification index (HLIFS), and origin using δ13C and δ15N. Nine soil health indicators were interrelated with SOM attributes. The primary forest presented the highest C stocks (107.7 Mg C ha−1), followed by 15- and 8-year-old restorations and pasture with 69.8, 55.5, and 41.8 Mg C ha−1, respectively. Soil C stocks from restorations and pasture were 20% lower than primary forest. However, 8- and 15-year-old restorations stored 12.3 and 28.3 Mg ha−1 more aboveground C than pasture. The younger forest had δ13C and δ15N values of 2.1 and 1.7‰, respectively, lower than the 15-year-old forest, indicating more C derived from C3 plants and biological N fixation. Both restorations and pasture had at least 34% higher HLIFS in deeper soil layers (10–30 cm) than primary forest, indicating a lack of labile SOM. Native and 15-year-old forests exhibited higher soil methane influx (141.1 and 61.9 μg m−2 h−1). Forests outperformed pasture in most soil health indicators, with 69% of their variance explained by SOM properties. However, SOM quantity and quality regeneration in both restorations approached the pristine forest state only in the top 10 cm layer, while deeper soil retained agricultural degradation legacies. In conclusion, active restoration of the Atlantic Forest is a superior approach compared to pasture reform for GHG mitigation. Nonetheless, the development of restoration techniques to facilitate labile C input into deeper soil layers (>10 cm) is needed to further improve soil multifunctionality and long-term C storage.

Screening Amazon rainforest plant extracts for antimicrobial activity: a 15-year commitment to the Brazilian biodiversity

IntroductionThe need for new tools to treat infections is constantly growing due to the possibilities of emerging diseases related to environmental changes, climatic catastrophes, microorganism resistance, and human and animal aging, leading to an evident unbalance in the planet’s health. Brazil contains the most significant portion of world biodiversity, a potential source of new antimicrobial natural products. Nonetheless, its environment, particularly its forests, and rainforests, is under threat, meaning that rapidly conducted, comprehensive research into the potential of antimicrobial activity to address this threat is urgently needed.MethodsIn this study, plants from the Amazon rainforest and the Atlantic forests were collected and tested against several pathogenic microbes relevant to humans, animals, and the environment, and subjected to large-scale susceptibility assays, bioautography, and Artemia salina toxicity assays. From the plants, 2,280 organic and aqueous extracts were obtained from different organs, namely leaves, barks, flowers, fruits, and seeds, and subjected to a large-scale susceptibility screening assay against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus mutans, Streptococcus sanguinis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Malassezia pachydermatis, Malassezia furfur, and Listeria monocytogenes. Results and discussionThe selected extracts were subjected to antimicrobial susceptibility tests to determine their inhibition zone diameters and minimum bactericidal concentrations, to bioautography, and to an Artemia salina toxicity assay, which resulted in 154 active extracts. Moreover, 111 out of 154 extracts were ranked based on scores established by the p-values and the mean rank differences in each set of test results. The final ranking identified which extracts should be studied in further phytochemical research using thin-layer chromatography techniques as a priority. The extracts obtained from plants belonging to Combretaceae, Connaraceae, Convolvulaceae, Fabaceae, Malpighiaceae, Moraceae, Piperaceae, Polygonaceae, and Salicaceae were selected as the most promising ones and used to support the identification of plant-based antimicrobial active compounds from the immense biodiversity of Brazilian forests.

Arbuscular mycorrhizal fungi associated with the rhizosphere of an endemic terrestrial bromeliad and a grass in the Brazilian neotropical dry forest.

Arbuscular mycorrhizal fungi form symbiotic associations with 80-90% of all known plants, allowing the fungi to acquire plant-synthesized carbon, and confer an increased capacity for nutrient uptake by plants, improving tolerance to abiotic and biotic stresses. We aimed at characterizing the mycorrhizal community in the rhizosphere of Neoglaziovia variegata (so-called `caroa`) and Tripogonella spicata (so-called resurrection plant), using high-throughput sequencing of the partial 18S rRNA gene. Both plants are currently undergoing a bioprospecting program to find microbes with the potential of helping plants tolerate water stress. Sampling was carried out in the Caatinga biome, a neotropical dry forest, located in northeastern Brazil. Illumina MiSeq sequencing of 37 rhizosphere samples (19 for N. variegata and 18 for T. spicata) revealed a distinct mycorrhizal community between the studied plants. According to alpha diversity analyses, T. spicata showed the highest richness and diversity based on the Observed ASVs and the Shannon index, respectively. On the other hand, N. variegata showed higher modularity of the mycorrhizal network compared to T. spicata. The four most abundant genera found (higher than 10%) were Glomus, Gigaspora, Acaulospora, and Scutellospora, with Glomus being the most abundant in both plants. Nonetheless, Gigaspora, Diversispora, and Ambispora were found only in the rhizosphere of N. variegata, whilst Scutellospora, Paraglomus, and Archaeospora were exclusive to the rhizosphere of T. spicata. Therefore, the community of arbuscular mycorrhizal fungi of the rhizosphere of each plant encompasses a unique composition, structure and modularity, which can differentially assist them in the hostile environment.

Antitumoral photoinduced effects of crude extract, fractions, and naphthoquinones from Sinningia magnifica (Otto & A. Dietr.) Wiehler (Gesneriaceae) in a bioguided study.

Photodynamic therapy (PDT) has been used for various purposes, including as an antitumor resource in a noninvasive therapy with minimal side effects. Sinningia magnifica (Otto & A. Dietr.) Wiehler is a rupicolous plant found in rock crevices in Brazilian tropical forests. Initial studies indicate the presence of phenolic glycosides and anthraquinones in species of the genus Sinningia (Generiaceae family). It is known that anthraquinones are natural photosensitizers with potential PDT applications. This led us to investigate the potential compounds of S. magnifica for use as a natural photosensitizer against the melanoma (SK-MEL-103) and the prostate cancer (PC-3) cell lines in a bioguided study. Our results showed that singlet oxygen production by the 1,3-DPBF photodegradation assay greatly increased in the presence of crude extract and fractions. The biological activity evaluation showed photodynamic action against melanoma cell line SK-MEL-103 and prostate cell line PC-3. These results suggest the presence of potential photosensitizing substances, as demonstrated in this in vitro antitumor PDT study by the naphthoquinones Dunniol and 7-hydroxy-6-methoxy-α-dunnione for the first time. Naphthoquinones, anthraquinones and phenolic compounds were identified in the crude extract by UHPLC-MS/MS analysis, motivating us to continue with the bioguided phytochemical study aiming to discover more photochemically bioactive substances in Gesneriaceae plants.

Stomatal conductance models in Brazilian forest plantations: genotype and environmental effects on eucalypt and pine forests

Stomatal conductance models in Brazilian forest plantations: genotype and environmental effects on eucalypt and pine forests

Trends of potentially illegal deforestation over protected areas of Rio das Velhas basin, Brazil

AbstractDespite the promise to increase control and fight against deforestation, the new Brazilian Forest Code (BFC) allowed more flexible dimensioning and land use rules in areas of private rural properties intended for native vegetation protection, namely the Legal Reserve (LR) and Areas of Permanent Preservation (APP). Following a decade of BFC implementation, the promise was somewhat flawed, while oversight and monitoring of private rural properties were not as expected. Considering this worrying scenario, the objective of this study was to quantify and map the forest balance of private rural properties listed in the Environmental Rural Registry of Rio das Velhas hydrographic basin (Minas Gerais, 2002), to assess compliance with the BCF and detect potentially illegal deforestation. The total deforested area in 6216 (28.7%) registered private rural properties reached approximately 119,800 ha after 2008, 61,500 ha of which were suppressed without surplus forest of LR. Besides, nearly half of the private rural properties presented forest deficits in the APPs. In a vast majority of cases, the deforestation was potentially illegal, but only a small number of private rural property owners was made responsible for that. The deforested areas were concentrated in small sectors within the rural properties, which would facilitate the control of illegal native vegetation suppression if oversight and law enforcement had been effective. Our results are an alert and a subside to public institutions and rural property owners while developing and executing plans, programs, and projects focused on environmental regularization of rural properties, with the ultimate goal of preventing further land degradation in the basin.

ECOLOGY OF TROPICAL FOREST SMALL MAMMAL POPULATIONS: PATTERNS AND PROCESS REVEALED BY THE LONGEST LONG-TERM MONITORING STUDY IN BRAZIL

Long-term monitoring studies have spearheaded the development of population ecology as a discipline. Today, the vast amount of information obtained from these studies is concentrated at higher-latitude ecosystems. Here we present the advances achieved by the largest long-term population monitoring study of small mammals carried out in a tropical forest in Brazil. Started in 1997 and with a total duration of 22 years, the Garrafão projectwas developed in one of the biggest Atlantic Forest remnants, in the southern limit of the Serra dos Órgãos National Park. Most studies developed with this valuable dataset have focused on the most abundant species of marsupials and rodents. In general, this long-term study brought important advances in the understanding of the population ecology of mammals in tropical forests, such as: (1) the predominance of population self-regulation in one of the most species-rich environments; (2) the importance of survival for population growth and regulation; (3) seasonal and continuous reproduction in marsupial rodents, respectively; (4) the positive relationship between body size and space use in marsupials weighing up to 100g (opposite relationship in species weighing less than 100g); (5) differences in space use between sexes and age classes, and positive (and negative) effects of density; and (6) differences in habitat selection in species living in different forest strata. Several other questions remain to be answered with this dataset and this review aims to stimulate the creation of new long-term monitoring studies in different ecosystems in the tropical region.

Cactus height increases the modularity of a plant–frugivore network in the Caatinga dry forest

AbstractCacti fruits are key resources to many frugivorous animals in Neotropical arid and semiarid regions. However, most studies have focused on a particular animal group or cacti species, but few have explored the overall interactions of such species at the community level. Here we monitored frugivory on five cacti species using camera traps that sampled diurnal and nocturnal interactions. We investigated the structure of interactions with bird, mammal, and reptile frugivores in the Brazilian Caatinga dry forest. We hypothesized that the height of cacti limit interactions with different types of frugivores, which would result in highly structured and modular interaction networks. In 2929 camera‐days, we recorded 23 vertebrate species feeding on cacti fruits, including seven new records, all determined to be primary seed dispersers. As predicted, the cacti‐frugivore network was modular and non‐nested, with the two shortest cacti species grouped in a module dominated by interactions with reptiles and non‐flying mammals. The tallest cacti species were dominated by frugivory interactions with birds and had comparatively less interaction diversity than shorter cacti species. Our results support the contention that cacti are keystone species in semiarid ecosystems where they produce small‐seeded fleshy fruits year‐round.

Wings are not perfect: increased wing asymmetry in a tropical butterfly as a response to forest fragmentation.

Habitat fragmentation and ecosystem changes have the potential to affect animal populations in different ways. To effectively monitor these changes, biomonitoring tools have been developed and applied to detect changes in population structure and/or individual traits that reflect such changes. Fluctuating asymmetry (FA) represents random deviations from perfect symmetry in bilateral traits from perfect symmetry in response to genetic and/or environmental stresses. In this study, we evaluated the use of FA as a tool to monitor stress caused by forest fragmentation and edge formation, using the tropical butterfly M. helenor (Nymphalidae) as a model species. We collected adult butterflies from three fragments of Atlantic Forest in Brazil encompassing both edge and interior habitats. Four wing traits (wing length, wing width, ocelli area, and ocelli diameter) were evaluated. Butterflies captured at edge sites exhibited higher FA values for wing length and wing width compared to those captured at interior sites, whereas traits related to ocelli did not show differences between the two habitat types. Our results suggest that the differences in abiotic and biotic conditions between forest interior and edges can act as a source of stress, impacting the symmetry of flight-related traits. On the other hand, as ocelli are crucial for butterfly camouflage and counter-predator strategies, our results indicate that this trait may be more conserved. By employing FA, we identified trait-specific responses to habitat fragmentation, thus suggesting its potential as a biomarker for environmental stress that can be used in butterflies to monitor habitat quality and change.

Wind farm noise and anuran diversity patterns: a case study in Brazilian seasonal dry tropical forest

ABSTRACT Noise pollution contributes to the global biodiversity crisis, however the consequences of this pollution on anuran diversity patterns are poorly understood. This is especially true of less evident sources of noise like wind farms and highly exploited areas, as in the Brazilian semi-arid region. Here, we evaluated the influence of wind farm noise on anuran assemblages’ diversity at the Caatinga, a seasonal dry tropical forest in Brazil. We tested the hypothesis that wind farm noise negatively affects the diversity of anuran assemblages in terms of abundance, species richness and composition. Anurans were sampled in 19 temporary ponds along a noise gradient in two wind farms over a rainy season (March to August). A total of 2,047 individuals belonging to 20 species were recorded. Our results suggest that wind farm noise has a non-significant relationship with anuran diversity patterns (species richness, composition and abundance). To our knowledge, this is the first investigation into the effects of wind farm noise on anuran assemblages in the Caatinga dry forest. Despite our results suggesting that anuran diversity is insensitive to noise pollution caused by wind farms, identification of emerging threats is essential to mitigate impacts on anuran populations which are declining globally.

Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation.

Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae), an edible species found in Brazilian Forest, possesses leaves that are traditionally used for the treatment of gastrointestinal disorders in Brazil. Extracts of C. lineatifolia are rich in phenolics and exhibit antioxidant, and gastric antiulcer properties. Furthermore, Campomanesia spp. have been described to possess anti-inflammatory properties, but studies related to chemical constituents of C. lineatifolia are scarce in the literature. This work aims to identify the chemical composition of the phenolic-rich ethanol extract (PEE) from C. lineatifolia leaves and evaluate the anti-inflammatory activity that could be related to its ethnopharmacological use. The high-speed countercurrent chromatography (HSCCC), using an isocratic and a step gradient elution method, and NMR, HPLC-ESI-QTOF-MS/MS were used to isolate and identify the chemicals of PEE, respectively. Lipopolysaccharide-(LPS)-stimulated THP-1cells were used to evaluate the anti-inflammatory activities from PEE and the two majority flavonoids isolated by measure TNF-α and NF-κB inhibition assays. Fourteen compounds were isolated from the PEE, further identified by NMR and HPLC-ESI-QTOF-MS/MS, twelve of them are new compounds, and two others are already known for the species. The PEE, quercitrin and myricitrin promoted a concentration-dependent inhibition of TNF-α, and PEE promoted an inhibition of NF-κB pathway. PEE from C. lineatifolia leaves demonstrated significant anti-inflammatory activity that may be related to the traditional use to treat gastrointestinal disorders.

Chemical Diversity and Redox Values Change as a Function of Temporal Variations of the Essential Oil of a Tropical Forest Shrub

This study investigated the chemical phenotypical variability of Piper lhotzkyanum Kunth (Piperaceae), a shrub found in Brazilian tropical forests, over time (different periods of the day and seasons) and under natural conditions. For this, essential oils (EOs) from the leaves were collected in different seasons and times of the day and analyzed by gas chromatography coupled with mass spectrometry, and gas chromatography coupled with a flame ionization detector. The indices were applied to evaluate the chemical diversity as well as the dynamics of redox of the mixtures. The results showed that the EOs were dominated by non-oxygenated sesquiterpenes, with β-elemene, E-caryophyllene, and α-zingiberene being the main compounds identified in all collections. Temporal and seasonal analyses revealed important fluctuations in the chemical composition, redox, and chemical diversity indices of the species. A correlation between climatic factors and the variation in redox and chemical diversity was observed, highlighting the chemical phenotypic plasticity P. lhotzkyanum. This study resolves a previously unanswered question by confirming that natural light does not produce interconversion of major compounds. The adaptation capacity of this species to the environmental changes suggests new cultivation strategies to maximize the quality of EO and promote a more sustainable future in partnership with nature.