Abstract Weasels are small mustelid carnivores that play an important role as predators of small mammals in a wide array of ecosystems. However, their response to land use, such as forest harvest for timber products, is seldom the subject of focused research and management projects. Both the American ermine, also known as the short-tailed weasel (Mustela richardsonii), and the long-tailed weasel (Neogale frenata) are native to Maine, United States, where commercial timber harvesting is widespread. The effects of this forest disturbance on weasels are poorly understood, so to contribute toward filling this knowledge gap, we conducted a 4-year, large-scale field study: specifically, our objective was to assess the effects of forest disturbance caused by timber harvest on occupancy patterns of ermines and long-tailed weasels occupancy patterns in Maine. We collected data from 197 survey sites (three camera traps each) over 4 years and analyzed over 7,000 images of weasels using dynamic false-positive occupancy models. We found that American ermines were widely distributed across the state (naïve occupancy at 54% of sites), while long-tailed weasels were rarer (naïve occupancy at 16% of sites). Both species responded positively to forest disturbance, with higher occupancy probabilities as disturbance increased, especially at the larger scales. American ermines were more likely to occupy stands with a higher percentage of conifer trees, while no such relationship was found for long-tailed weasels. We conclude that current forest harvest practices in Maine are not detrimental to weasel populations, but that the two species warrant continued monitoring.