Eucalyptus plantation forests in southern China provide not only the economic value of producing timber, but also the ecological value service of absorbing carbon dioxide and releasing oxygen. Based on the theory of spatial colonial modeling, this paper proposes a new method for 3D reconstruction of tree terrestrial LiDAR point clouds for determining the aboveground carbon stock of eucalyptus monocotyledons, which consists of the main steps of tree branch and trunk separation, skeleton extraction and optimization, 3D reconstruction, and carbon stock calculation. The main trunk and branches of the tree point clouds are separated using a layer-by-layer judgment and clustering method, which avoids errors in judgment caused by sagging branches. By optimizing and adjusting the skeleton to remove small redundant branches, the near-parallel branches belonging to the same tree branch are fused. The missing parts of the skeleton point clouds were complemented using the cardinal curve interpolation algorithm, and finally a real 3D structural model was generated based on the complemented and smoothed tree skeleton expansion. The bidirectional Hausdoff distance, average Hausdoff distance, and F distance were used as evaluation indexes, which were reduced by 0.7453 m, 0.0028 m, and 0.0011 m, respectively, and the improved spatial colonization algorithm enhanced the accuracy of the reconstructed tree 3D structural model. To verify the accuracy of our method to determine the carbon stock and its related parameters, we cut down 41 eucalyptus trees and destructively sampled the measurement data as reference values. The R2 of the linear fit between the reconstructed single-tree aboveground carbon stock estimates and the reference values was 0.96 with a CV(RMSE) of 16.23%, the R2 of the linear fit between the trunk volume estimates and the reference values was 0.94 with a CV(RMSE) of 19.00%, and the R2 of the linear fit between the branch volume estimates and the reference values was 0.95 with a CV(RMSE) of 38.84%. In this paper, a new method for reconstructing eucalyptus carbon stocks based on TLS point clouds is proposed, which can provide decision support for forest management and administration, forest carbon sink trading, and emission reduction policy formulation.
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