Forensic Identification Research Articles

Assessing the reliability of the forensic technique for the identification study of motor gasoline using gas-liquid chromatography

A new approach to solving the problems of forensic examination of petroleum products, fuels and lubricants associated with establishing whether motor gasoline belongs to a common/different identification set is proposed. A methodology for the identification of gasoline using quantitative results of chromatographic determination of controlled parameters and subsequent evaluation of the results in pairwise compared samples according to the established criteria. The following parameters were selected as controlled indicators: values of research octane number (RON), concentrations of hydrocarbon groups (paraffins, isoparaffins, arenes, naphthenes, olefins) and oxygenates. Their determination was carried out using standardized methods. We used the hardware and software complex of a Chromatek-Crystal 5000 series, including the Chromatek-DHA data processing program. Estimated criteria or decision- making rules regarding the issues posed to an expert were determined to classify gasoline under one product name (generic set), one production technology (group set) or a common source of origin (one production batch, storage tank, etc.). The reliability of the method was assessed using a validation procedure consisted of three stages. We used a collection of AI-92 motor gasoline, selected at gas stations of four oil companies during six months of 2022 in various districts of Moscow. At the first stage, the performer analyzed 12 aliquots of each of four gasoline samples (samples that previously belonged to the common volume) at different times. It was established that the quality indicators of the proposed methodology (standard deviation of the repeatability and reproducibility, expanded uncertainty, limits of the repeatability and reproducibility) for each of the determined controlled indicators did not exceed the permissible errors established by standardized methods. At the second stage of the experiment, the performer combined 12 test samples into four groups from each manufacturer (one production technology) with three samples of different production times (different batches). By comparing the discrepancies in the measured values of the same controlled indicators between pairs of samples within groups and the discrepancy in average indicators between pairs of groups, the contractor identified gasolines manufactured using the same technology; gasolines produced by the same technology, but at different times (different batches) and, with a probability of 95%, gasolines having a common source of origin (previously belonging to a common volume). The conclusions of the validation study matched the original data on the samples, which confirmed the correctness of the developed comparison criteria. At the third stage (blind test), the performer examined seven gasoline samples of, information about the composition and properties of which was not provided to him. The results of the blind test were considered satisfactory. Thus, the validation results indicate the suitability of the methodology for solving forensic identification problems in relation to motor gasoline and the competence of the performer sufficient for implementation of the methodology.

Blood alcohol concentration in the clinical laboratory: a narrative review of the preanalytical phase in diagnostic and forensic testing.

The analysis of blood alcohol concentration (BAC), a pivotal toxicological test, concerns acute alcohol intoxication (AAI) and driving under the influence (DUI). As such, BAC presents an organizational challenge for clinical laboratories, with unique complexities due to the need for forensic defensibility as part of the diagnostic process. Unfortunately, a significant number of scientific investigations dealing with the subject present discrepancies that make it difficult to identify optimal practices in sample collection, transportation, handling, and preparation. This review provides a systematic analysis of the preanalytical phase of BAC that aims to identify and explain the chemical, physiological, and pharmacological mechanisms underlying controllable operational factors. Nevertheless, it seeks evidence for the necessity to separate preanalytical processes for diagnostic and forensic BAC testing. In this regard, the main finding of this review is that no literature evidence supports the necessity to differentiate preanalytical procedures for AAI and DUI, except for the traceability throughout the chain of custody. In fact, adhering to correct preanalytical procedures provided by official bodies such as European federation of clinical chemistry and laboratory medicine for routine phlebotomy ensures both diagnostic accuracy and forensic defensibility of BAC. This is shown to depend on the capability of modern pre-evacuated sterile collection tubes to control major factors influencing BAC, namely non-enzymatic oxidation and microbial contamination. While certain restrictions become obsolete with such devices, as the use of sodium fluoride (NaF) for specific preservation of forensic BAC, this review reinforces the recommendation to use non-alcoholic disinfectants as a means to achieve "error-proof" procedures in challenging operational environments like the emergency department.

Sequenced-based Rwanda population provides insights into demographic history.

Rwanda is known as the heart of Africa, reflecting the history of the world. Colonization and genocide have led to Rwanda's existing genetic structure. Herein, we used massively parallel sequencing to analyze 296 loci in 185 Rwandans and constructed a database for Rwandan forensic data for the first time. We found the following results: First, forensic parameters demonstrated that all loci were highly informative and could be used for forensic identification and paternity tests in Rwandans. Second, we found that the differences in genetic background between Rwandans and other African populations were similar but slight, as indicated by the massively parallel sequencing panel. Rwandans belonged to the African population and were inseparable from populations from neighboring countries. Also, Rwandans were closer to the European and American populations because of colonization, war, and other reasons. There was no scientific basis for racial classification established by colonization. Further research still needs to be carried out on more loci and larger Rwandan samples.

A practical workflow for forensic species identification using direct sequencing of real-time PCR products

A practical workflow for forensic species identification using direct sequencing of real-time PCR products

Evaluating the patella bone for sex estimation in Northwest Indian subjects: A radiological study

ObjectivesIn forensic identification, pelvis, post cranial bones especially long bones and skull are considered best for sex estimation. But there are circumstances where these bones cannot be recovered for establishing the correct biological profile of unknown human skeletal remains. In those cases, other skeletal bones should be investigated for sex estimation. The goal of the present study was to validate whether the patellar bone measurements through CT scan can be used for the sex estimation in Northwest Indian subjects. Materials and methodsThis study comprises of 344 subjects (179 males and 165 females) between the age group of 18- 80 years. Eight variables of patella were selected in the study for sex estimation using CT scan images of patella. ResultsAll the variables were statistically significant with p-value< 0.05 for indicating the sex. Univariate and multivariate discriminant function analysis (direct and stepwise method) were applied for sex estimation. The overall accuracy rate of classifying sex was 88.4% for multivariate stepwise discriminant function analysis and 87.8% for multivariate direct discriminant function analysis. Total perimeter of patella (TPP) was found to be the best variable for sex estimation with overall accuracy rate of 84.9%. ConclusionThe present study emphasized on the most dimorphic variables of patella in Northwest Indian subjects. New variables of patella namely Patellar Femoral Distance (PFD), Patellar Apex Angle (PAA), Frontal Surface Area of patella (FSA) and Total Perimeter of Patella (TPP) were added in the study of Indian subjects. This brought an overall accuracy rate of classification above 85%. The study confirmed that patellar bone's linear and angular measurements can be used for forensic identification casework.

The effect of tea catechins on the forensic identification of urine: Urine camouflage to evade drug tests

BackgroundWe encountered a urine sample suspected of being mixed with tea, submitted by a suspect attempting to camouflage illegal drugs. Although urine should turn reddish-pink during a urea test with p-Dimethylaminocinnamaldehyde (DAC), this suspect’s sample exhibited a blue coloration when tested with DAC. AimOur aim was to examine the influence and mechanism of green tea on various urine identification tests. ResultsOur examination revealed that DAC forms a compound with the urea in urine, resulting in a reddish pink coloration with a molecular weight of 217. However, it has been reported that DAC binds to polyphenols such as catechin. In the case of catechin, DAC binds to the C8 position, forming a compound that exhibits the highest absorption at 640 nm and appears blue. we investigated the effect of urine from volunteers who had consumed a large amount of catechin on the urea test with DAC. Additionally, we carried out quantitative analysis of catechin in urine by LC-MS/MS after enzymatic treatment with β-glucuronidase. The concentration of urinary excreted catechin reached its peak approximately 3 to 4 h after ingestion. During the DAC test, urine samples collected 3 to 4 h after catechin ingestion displayed a bluish pink color, but not the blue color observed in the original suspect sample. ConclusionThis study investigated the impact of catechin on urine tests, revealing that a blue color in the DAC test indicates a high likelihood of camouflage by the suspect.

Prediction of BMI traits in the Chinese population based on the gut metagenome

BackgroundIdentifying individual characteristics based on trace evidence left at a crime scene is crucial in forensic identification. Microbial communities found in fecal traces have high individual specificity and could serve as potential markers for forensic characterization. Previous research has established that predicting body type based on the relative abundance of the gut microbiome is relatively accurate. However, the long-term stability and high individual specificity of the gut microbiome are closely linked to changes at the genome level of the microbiome. No studies have been conducted to deduce body shape from genetic traits. Therefore, in this study, the vital role of gut bacterial community characteristics and genetic traits in predicting body mass index (BMI) was investigated using gut metagenomic data from a healthy Chinese population.ResultsRegarding the gut microbial community, the underweight group displayed increased α-diversity in comparison to the other BMI groups. There were significant differences in the relative abundances of 19 species among these three BMI groups. The BMI prediction model, based on the 31 most significant species, showed a goodness of fit (R2) of 0.56 and a mean absolute error (MAE) of 2.09 kg/m2. The overweight group exhibited significantly higher α-diversity than the other BMI groups at the level of gut microbial genes. Furthermore, there were significant variations observed in the single-nucleotide polymorphism (SNP) density of 732 contigs between these three BMI groups. The BMI prediction model, reliant on the 62 most contributing contigs, exhibited a model R2 of 0.72 and an MAE of 1.56 kg/m2. The model predicting body type from 44 contigs correctly identified the body type of 93.55% of the study participants.ConclusionBased on metagenomic data from a healthy Chinese population, we demonstrated the potential of genetic traits of gut bacteria to predict an individual’s BMI. The findings of this study suggest the effectiveness of a novel method for determining the body type of suspects in forensic applications using the genetic traits of the gut microbiome and holds great promise for forensic individual identification.

Machine Learning Models for Prediction of Sex Based on Lumbar Vertebral Morphometry.

Identifying skeletal remains has been and will remain a challenge for forensic experts and forensic anthropologists, especially in disasters with multiple victims or skeletal remains in an advanced stage of decomposition. This study examined the performance of two machine learning (ML) algorithms in predicting the person's sex based only on the morphometry of L1-L5 lumbar vertebrae collected recently from Romanian individuals. The purpose of the present study was to assess whether by using the machine learning (ML) techniques one can obtain a reliable prediction of sex in forensic identification based only on the parameters obtained from the metric analysis of the lumbar spine. This paper built and tuned predictive models with two of the most popular techniques for classification, RF (random forest) and XGB (xgboost). Both series of models used cross-validation and a grid search to find the best combination of hyper-parameters. The best models were selected based on the ROC_AUC (area under curve) metric. The L1-L5 lumbar vertebrae exhibit sexual dimorphism and can be used as predictors in sex prediction. Out of the eight significant predictors for sex, six were found to be particularly important for the RF model, while only three were determined to be important by the XGB model. Even if the data set was small (149 observations), both RF and XGB techniques reliably predicted a person's sex based only on the L1-L5 measurements. This can prove valuable, especially when only skeletal remains are available. With minor adjustments, the presented ML setup can be transformed into an interactive web service, freely accessible to forensic anthropologists, in which, after entering the L1-L5 measurements of a body/cadaver, they can predict the person's sex.

Morphometry of proximal femur and correlations of the head neck shaft assemblage

Introduction: Recent times have shown an increased incidence of proximal femur pathologies. The morphology and morphometry of the proximal femur is an important component of hip biomechanics. Altered diameters and angles affect the range of motion and have been found to be causative in hip pathologies. Understanding femoral shape and orientation can help to predict degenerative diseases, relate to hip instability, in designing hip replacement implants, in preoperative planning and for forensic identification of human remains. This study aimed at determining the morphometric parameters of proximal femur and to analyse the correlations between measured parameters. Materials and Methods: Measurements were made on 120 dry adult human femora of unknown sex (Right-60, Left-60). Transverse and vertical diameters of the head and neck and anterior and posterior neck lengths were measured using digital vernier calliper and neck-shaft angle was measured using a goniometer. Measurements were statistically analysed. Correlation coefficient was used to analyse the relationship among variables. Results: Mean vertical and transverse diameters of head were 43.23 mm and 43.16 mm and Mean Anterior and Posterior neck lengths were 28.37 mm and 35.81 mm respectively. Mean Neck shaft angle was 128.82. On comparison between sides, statistically significant differences were observed in neck shaft angle and anterior neck length measurements. Correlation between neck shaft angle and neck length as well as between neck shaft angle and head diameters revealed variable patterns on left and right sides. Conclusions: Our study has found a significant difference in anterior length of neck in left and right femur as well as a substantial and statistically significant positive correlation was observed between anterior neck length and neck shaft angle of the right femur. Keywords : Femur, Morphometry, Vernier, Goniometer, Hip, Prosthesis

Systematic evaluation of the Precision ID GlobalFiler™ NGS STR panel v2 using single-source samples of various quantity and quality and mixed DNA samples

Massively parallel sequencing (MPS) techniques were developed approximately 15 years ago. Meanwhile, several MPS kits for forensic identification, phenotypic information, ancestry, and mitochondrial DNA analysis have been developed and their use has been established. Sequencing short tandem repeats (STRs) has certain advantages over the currently used length-based genotyping methods, which are based on PCR amplification followed by capillary electrophoresis (CE). MPS is more discriminative and includes the possibility of testing high numbers of targets (> 100), different types of markers [STRs and single nucleotide polymorphisms (SNPs)], as well as the use of smaller amplicons (< 300 bp). This study evaluated in 24 experimental runs the Precision ID GlobalFiler™ NGS STR panel v2 from ThermoFisher, which targets 31 autosomal STRs, amelogenin, and three Y-markers (one STR, SRY, and Yindel). Single-source samples were used in 18 experimental runs, for systematic evaluation. These included assessing library preparation benchmark conditions, limited DNA input, as well as testing repeatability, number of samples per run, and degraded DNA samples. Full profiles were consistently obtained from as little as 50 pg DNA input. Using the optional recovery PCR method improved outcomes for samples with low DNA input. Full profiles were also obtained from severely degraded DNA samples with degradation indices (DI) of > 60. In addition, six experimental runs were performed testing various two-person mixtures with mixture ratios ranging from 1:20 to 20:1. Major and minor contributors were distinguishable by their read counts (coverage), because less DNA input yielded lower read counts, analogous to the traditional CE technology, where less DNA produces lower peak heights. Mixture ratios of approximately 1:1 were indistinguishable, while a greater imbalance, i.e., higher mixture ratios, made the mixture more distinguishable between major and minor contributors. Based on this information, the highest success rate of correctly deconvoluted four-allelic loci was from mixtures with 1:3 ratios. At higher mixture ratios, the drop-out rate of the minor contributor increased, reducing the number of four-allelic loci.

Short tandem repeat (STR) variation from 6 cities in Iraq based on 15 loci

BackgroundOne thousand sixty-one individuals were sampled from the cities of Anbar, Baghdad, Basra, Diyala, Najaf, and Wasit in Iraq and typed for 15 forensic STRs to explore the genetic structure of Iraq and develop a forensic DNA database. The total number of alleles that were identified was 203. ResultAnalyses of molecular variance (AMOVA) were then conducted Baghdad provides a good representation of the rest of the country, while Anbar is the most genetically distinct. The average heterozygosities of these loci was 0.779, homozygosities was 0.221, polymorphism information content was 0.77, power of discrimination was 0.927, and power of exclusion was 0.563. At these loci, a matching genotype will occur, on average, in 1 in 8.152 × 1017 individuals. For paternity tests, the average paternity probability for a matching profile is 99.9997%. ConclusionsThese loci are appropriate for use in forensic and paternity testing for this population. Iraq is similar to other countries in the Middle East, particularly Iran and Turkey, and is more similar to Europe than either Asia or Africa.

Multidimensional foot measurements-based sex estimation for Taiwanese population in foot length overlapping interval

ABSTRACT Estimating the sex from unknown bodies or fragmented human remains is essential for forensic identification. For sex estimation from foot features, previous studies highly depend on the foot length, resulting in a single-dimensional threshold and thusly poor performance for data falling into the foot length overlapping interval. Moreover, available information about the sex estimation from foot dimensions among Taiwan population is lacking. Hence, this study proposes a novel method to achieve multidimensional foot measurements-based sex estimation for the human falling into the overlapping foot interval. In total, 780 men and 670 women with same foot lengths ranging from 23.0 to 25.5 cm were recruited. A three-dimensional foot scanner was used to measure 10 dimensions of both feet. Bilateral differences were detected in several foot dimensions under the same foot length categories. The support vector machine method provided better results in terms of accuracy (86.05%), recall (86.44%), specificity (85.59%), and precision (83.77%). This study indicates the importance of other variables including foot length, ball girth, instep girth, ball of foot length, and outside ball of foot length in sex estimation. The findings will provide helpful information for forensic practice for Taiwanese and extend the application range of machine learning methods.

Characterization of DNA Quality, TPOX Locus, TH01 Locus in Seawater Soaked Nails in Forensic Identification

Forensic science is multiplying. One of them is the identification of events at sea. Of course, the victim’s body can be submerged in seawater for days so that components in seawater can affect the victim’s body. This research aimed to see the effect of storage time on the level and purity of DNA in nail samples. Identification of nail DNA using the Polymerase Chain Reaction (PCR) method. The DNA purity test examines nail samples using a spectrophotometer read at 260 and 280 nm wavelengths. Measurement of DNA levels using the PCR instrument on the STR TPOX locus, the result was 32.02 ug/uL on positive control, 36.73 ug/uL in seawater immersion on the 2nd day, 35.35 ug/uL in seawater immersion on the 7th day, and 34.5 ug/uL in seawater immersion on the 20th day. At the STR TH01 locus, the result was 32.155 ug/uL on positive control, 38.05 ug/uL on the 2nd day of immersion in seawater, 35.18 ug/uL on the 7th day, and 33.88 ug/uL on the 20th day. Results Examination of the Total Plate Number of seawater on the 2nd day, 7th day, and 20th day of immersion found no colonies of germ colony growth. This study obtained sufficient DNA purity from the 2nd day, 7th day and 20th day. So that the samples could be analyzed for DNA levels, the result obtained from the study showed a decrease in DNA levels at the TPOX Locus and TH01 Locus from the 2nd day, 7th day, and 20th day.

Gender Determination Based on CBCT Maxillary Sinus Analysis: A Systematic Review

Gender determination is an essential element for human identification in forensic medicine, to which the maxillary sinuses may contribute as they remain intact even after severe damage to the skull and other structures. Aim: To evaluate scientific evidence published over the last decade to determine whether maxillary sinus dimensions and volume may constitute useful parameters for forensic identification and gender determination, based only on cone-beam computed tomography images (CBCT). Methods: This review adhered to the PRISMA statement’s criteria. Four databases were searched for articles published between January 2010 and April 2023. Results: Initially, 1719 records were identified. After screening, there were 2475 participants in the included studies. Of the fifteen articles selected, five reported data assessing only volumetric measurements of the maxillary sinus, seven reported data calculating only linear measurements and three reported data by combining findings of both linear and volumetric measurements of the maxillary sinus. Maxillary sinus volume was significantly higher in male participants. Maxillary sinus height was the best discriminating parameter for forensic identification with an overall accuracy ranging from 70% to 80%. Conclusions: Maxillary sinus measurements revealed anatomic variability between genders, and this approach can be applied as a complementary method for human identification.

Fine alignment, flexible fusion: A novel framework of multi-model score fusion in face identification

Multi-model score fusion was considered a bottleneck problem in forensic face identification. While the score distribution of different face models varies greatly, the existing score processing methods cannot achieve accurate alignment. This paper proposed a score fusion framework named fine alignment and flexible fusion framework (FAFF). In FAFF, we took score-based likelihood ratios as the reference values to align the similarity scores generated by different face models. First, we set up a unified calibration test workflow based on the forensic likelihood ratio test. Then, 3 LLR anchor-based methods (LLRBA1, LLRBA2, and LLRBA3) and LLR curve-based methods (LLRBC) were proposed. Finally, we conducted fusion experiments on four face models (VGGface, Facenet, Arcface, and SFace). The experimental results show that on the CelebA dataset, compared with the existing MOEBA and PAN methods, LLRBC increased the TPR@ 10−7 FPR by 175.4 % and 162.9 %, and LLRBA increased by 55.6 % and 48.5 %.

Age-Related Changes to Frontal Sinus Traits and Implications for Forensic Identification

Forensic studies on frontal sinus identification are often limited to adult samples or utilize static, simulated antemortem images, which overlook any potential temporal changes in sinus morphology. Further, studies on frontal sinus growth typically utilize lateral radiographs and/or are cross-sectional. The current study utilizes a longitudinal sample of frontal radiographs to determine the age at which forensically relevant frontal sinus traits stabilize during growth and development. The sample includes 1500 radiographs of 141 individuals (66F/75M) ranging from three to 56 years of age (yoa). For each individual, trait age-of-stabilization was recorded by identifying the year at which each coded trait became consistent across images.Our results demonstrate that frontal sinus traits stabilize on average 10–15yoa, with sinus presence being the first to stabilize and arcade counts the last. Females generally stabilized earlier (9–14yoa) versus males (10–15yoa). However, sex differences were generallynot statistically significant. Further, traits displayed a high degree of variation with wide standard deviations (~3 years). However, by 21yoa almost all individuals displayed stabilization in all traits, suggesting that little change should be expected with later-aged postmortem radiographs. Still, given the amount of variation, forensic practitioners should be cautious using frontal sinus identification methods in subadults, especially when years may have elapsed between images. When conducting a radiographic comparison that involves a subadult antemortem image, the results of this study may help the practitioner interpret whether the differences between antemortem and postmortem radiographs can be explained by age and time elapsed between radiographs.

Allelic frequencies of 22 short tandem repeats loci and tri-allelic patterns of Penta D and TPOX identified in Gabonese population

Short tandem repeats (STRs) are repeating DNA sequences used in forensic human identity testing and the diagnosis of aneuploidies. Many STRs like Penta D and TPOX are used routinely for paternity tests, but these tests are not widely used in sub-Saharan Africa. In this study we recruited individuals from Gabonese families seeking a paternity test. After DNA extraction from buccal swabs, we genotyped samples using a panel of 22 STRs. A total of 115 unrelated subjects from 39 families were included. Allele frequencies of the 22 STR loci were determined in unrelated Gabonese subjects. The most polymorphic loci were D21S11 (16 alleles) and FGA (17 alleles), while D3S1358 and TH01 loci were less polymorphic, with five alleles each. Deviation from Hardy–Weinberg equilibrium was observed for TPOX, D3S1358, CSFPO and D7S820 loci. We reported tri-allelic patterns that indicate aneuploidies at a combined frequency of 4% (4/115) with 3% for Penta D (1/35) and 3% for TPOX (3/102). Furthermore, we identified a new tri-allelic genotype 5-8-16 for the Penta D locus located on chromosome 21 in a healthy subject. In addition, we observed three tri-allelic variants of TPOX, located on chromosome 2, in healthy subjects, namely 8-10-11, 8-9-10, and 8-8-10. Our study revealed unsuspected polymorphic variations in Penta D and TPOX for the first time in Gabon, raising several questions about chromosomal disorders. Further population genetics studies are needed in Gabon to better characterize these variations, both qualitatively and quantitative.

Forensic analysis of the Turkey 2023 presidential election reveals extreme vote swings in remote areas

Concerns about the integrity of Turkey’s elections have increased with the recent transition from a parliamentary democracy to an executive presidency under Recep Tayyip Erdoğan. Election forensics tools are used to identify statistical traces of certain types of electoral fraud, providing important information about the integrity and validity of democratic elections. Such analyses of the 2017 and 2018 Turkish elections revealed that malpractices such as ballot stuffing or voter manipulation may indeed have played a significant role in determining the election results. Here, we apply election forensic statistical tests for ballot stuffing and voter manipulation to the results of the 2023 presidential election in Turkey. We find that both rounds of the 2023 presidential election exhibit similar statistical irregularities to those observed in the 2018 presidential election, however the magnitude of these distortions has decreased. We estimate that 2.4% (SD 1.9%) and 1.9% (SD 1.7%) of electoral units may have been affected by ballot stuffing practices in favour of Erdoğan in the first and second rounds, respectively, compared to 8.5% (SD 3.9%) in 2018. Areas with smaller polling stations and fewer ballot boxes had significantly inflated votes and turnout, again, in favor of Erdoğan. Furthermore, electoral districts with two or fewer ballot boxes were more likely to show large swings in vote shares in favour of Erdoğan from the first to the second round. Based on a statistical model, it is estimated that these shifts account for 342,000 additional ballots (SD 4,900) or 0.64% for Erdoğan, which is lower than the 4.36% margin by which Erdoğan was victorious. Our results suggest that Turkish elections continue to be riddled with statistical irregularities, that may be indicative of electoral fraud.

‘Strands in a cable’: effective investigator decision-making using forensic identification evidence in volume crime investigations

ABSTRACT Police investigators increasingly make use of forensic science in the investigation of crime. While there is considerable research on case outcomes following the use of forensic identification evidence (fingerprint and DNA evidence), few studies have explored how police investigators use these evidence types in their investigations. This study aimed to examine police investigators’ reasoning processes about the use of forensic identification evidence in volume crimes, such as burglary, to develop a decision-making framework that can be applied to the investigation of such crimes. Twenty-four police officers from three Australian police jurisdictions participated in semi-structured interviews that centred around a case scenario. Data were transcribed verbatim and analysed thematically. The findings highlight that police investigators’ decision-making is influenced by the requirement to meet the rules of evidence. Further, participants’ own experience and mentoring by more experienced colleagues influenced not only the decisions made in a case, but also the development of decision-making skills in the use of forensic evidence more broadly. A decision-making framework is proposed to explain and guide the use of forensic evidence in volume crime investigations. Overall, the findings suggest that the effective use of forensic identification evidence in volume crime investigations requires that police investigators engage actively in the decision-making process. Further research can explore ways to integrate the findings from this research into police practices.

ANATOMICAL STUDY OF NUTRIENT FORAMINA IN LONG BONES OF HUMAN UPPER AND LOWER LIMBS

Aims and Objectives: The objectives of the study are as follows: (1) To determine the number, location, position, and direction of nutrient foramina in the shaft of long bones. (2) To determine the foraminal index of the long bones. Methods: This was a cross-sectional study, undertaken on dry cadaveric human long bones of unknown age and sex from the Department of Anatomy, M.S. Ramaiah Medical College, Bangalore. The duration of study was 2 years. In this study, 350 human long bones which include the clavicle, humerus, radius, and ulna from the upper extremity; femur, tibia, and fibula from the lower extremity were examined in detail for the number, position, location, and directions of the nutrient foramen. For statistical purposes, p&lt;0.05 was taken as significant. Results: All the bones had single nutrient foramina and a higher percentage of double nutrient foramina was seen in femur. The most common position was the middle one-third of the shaft and the surface distribution was different in different bones. All the bones had the nutrient foramina, which were directed away from the growing end. The mean foraminal index for clavicle, humerus, radius, and ulna was 52.85±9.24, 56.92±6.57, 34.80±6.07, and 36.0±5.85, respectively. Mean foraminal index for femur, tibia, and fibula was 43.54±10.32, 32.37±3.1, and 51.68±9.77. Conclusion: Knowledge of nutrient foramina of long bones is crucial for orthopedic surgery, forensic identification, obtaining vascularized bone grafts, and treating trauma or malignant bone conditions.