IntroductionNowadays in Mexico the use of short tandem repeats has increased in Forensic practice for individual identification, due to its excellent discriminative power and its widespread use in laboratories around the world. ObjectiveThe aim of this research work was to delve into the knowledge of allelic frequencies from markers such as D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA in the metropolitan area in the central region of Mexico. Material and methodsA study about the allelic frequencies obtained from 300 healthy and unrelated individuals was developed. Data were analyzed statistically. Results and conclusionsThe combined power of discrimination was 0.999999999. All loci are in Hardy-Weinberg equilibrium, and show high discrimination for paternity analysis and forensic genetic applications. Results contribute to establishing a representative database of genome admixture in the region, and show the marked genetic heterogeneity that characterizes Latin American populations.