Assimilation experiments are performed with the Weather Research and Forecasting (WRF) models’ three-dimensional variational data assimilation (3D-Var) scheme to evaluate the impact of directly assimilating the Advanced Television and Infrared Observation Satellite Operational Vertical Sounder (ATOVS) radiance, including AMSU-A, AMSU-B and HIRS, on the analysis and forecasts of a mesoscale model over the Indian region. The present study is, to our knowledge, the first where the impact of ATOVS radiance has been evaluated on the analysis and forecasts of a mesoscale model over the Indian region. The control (without ATOVS radiance) as well as experimental (which assimilated ATOVS radiance) run were made for 48 h starting at 0000 UTC during the entire July 2008. The impacts of assimilating the radiances from different instruments (e.g., AMSU-A, AMSU-B and HIRS) were measured in comparison to the control run. The assimilation experiments for July 2008 (30 cases) demonstrated a positive impact of the assimilated ATOVS radiance on both the analysis state as well as subsequent short-range forecasts. Relative to the control run, the moisture analysis was improved with the assimilation of AMSU-B and HIRS radiance, while AMSU-A was mainly responsible for improved temperature analysis. The comparison of the model-predicted temperature, moisture and wind with NCEP analysis indicated that a positive forecast impact is achieved from each of the three instruments. HIRS and AMSU-A radiance yielded only a slight positive forecast impact, while AMSU-B radiance had the largest positive forecast impact for moisture, temperature and wind. The comparison of model-predicted rainfall with observed rainfall indicates that ATOVS radiance, particularly AMSU-B and HIRS, impacted the rainfall positively. This study clearly shows that the improved analysis of mid-tropospheric moisture, due to the assimilation of AMSU-B radiances, is a key factor to improve the short-term forecast skill of a mesoscale model.